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Speciation of the elements and compositions on the surfaces of dust storm particles:The evidence for the coupling of iron with sulfur in aerosol during the long-range transport

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Chinese Science Bulletin 2005年第8期50卷 738-744页

作者： ZHANG Xingying1,2, ZHUANG Guoshun1,2,3. CHEN Jianmin2 & XUE Huaxin2 1. Center for Atmospheric environment Study, Department of Chemistry, Beijing Normal University, Beijing 100875, china 2. Center for Atmospheric Chemistry Study, Department of Environ- mental Science and engineering, Fudan University, Shanghai 20043., china 3. LAPC/NZC, institute of Atmospheric Physics, NZC/LAPC, Chinese Academy of Sciences, Beijing 100029, china Center for Atmospheric environment Study Department of Chemistry Beijing Normal University Beijing 100875 China Center for Atmospheric Chemistry Study Department of Environmental Science and Engineering Fudan University Shanghai 200433 China LAPC/NZC Institute of Atmospheric Physics NZC/LAPC Chinese Academy of Sciences Beijing 100029 China

The speciation of the elements on the surface of the particles collected during dust storm and non-dust storm in Beijing and Inner Mongolia was studied by XPS. The ma- jor species of iron on the surface were oxides, sulfate, silicate, FeOOH and minor part sorbed on SiO2/Al2O3. Sulfate is the dominant species of sulfur on the surface. SiO2 and Al2O3.are the main components of Si and Al on the surface respectively. One of the most important findings was that the Fe(II) (FeS and FeSO4) produced could account for up to 44.3. and 45.6% of the total Fe on the surface in the aerosol sample collected at that night and next day of the “peak” time of the dust storm occurring on March 20, 2002, while Fe2(SO4)3. one of the Fe(III) species on the surface decreased from 67.1% to 49.5% and 48.0% respectively. Both S and Fe en- riched on the surface of aerosol particles. Fe(II) accounted for 1.3.?5.3. of total Fe in bulk aerosol samples during dust storm. These results provided strong evidence to sup- port the hypothesis of the coupling between iron and sulfur in aerosols during the long-range transport, which would have important impact on the global biogeochemical cycle.

关键词：物种形成原理 XPS 大气悬浮物 Fe-S结合粒子集合

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Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon

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Chinese Science Bulletin 2006年第17期51卷 2059-2064页

作者： LIU Bin1,2,3.4, WANG Kemin1,2,3.4, XIAO Zhiqiang5,6, WANG Wei1,3.4, TAN Weihong1,2,3.4, SUN Yi5,6, TANG Hongxing1,3.4 & YANG Xiaohai1,3.41. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hu- nan University, Changsha 410082, china 2. Life Science and Biotechnology institute, Hunan University, Chang- sha 410082, china 3. Chemistry and chemical Engineer College, Hunan University, Changsha 410082, china 4. Hunan engineering Research Center for Bio-Nanotechnology, Hunan University, Changsha 410082, china 5. Medical Experiment Research Center, Xiangya Hospital, Central South University, Changsha 410008, china 6. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Changsha 410008, china State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha China Life Science and Biotechnology institute Hunan University Changsha China Chemistry and chemical Engineer College Hunan University Changsha China Hunan engineering Research Center for Bio-Nanotechnology Hunan University Changsha China Medical Experiment Research Center Xiangya Hospital Central South University Changsha China Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Changsha China

The effects of drug treatment, gene transfection, and p53.RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5-FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p53.with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 53.4× 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigat- ing the interaction of multigenes on signal transduc- tion pathway.

关键词： ING1 RT-PCR 定量分析分子信号 mRNA

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Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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Science china(Life Sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3.ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3.&ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,china 2 Department of Chemistry and chemical {3.,Hunan institute of {3.,Xiangtan 411104,china 3.College of Life Science,Capital Normal University,Beijing 10003.,china 4 College of Life Science,Zhongkai University of Agriculture and technology,Guangzhou 510225,china 5 College of Grassland Science,Gansu Agricultural University,Lanzhou 73.070,china 6 Department of Biology,Henan institute of Science and technology,Xinxiang 453.03.china 1. Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and chemical engineering Hunan Institute of Engineering Xiangtan 411104 China 3. College of Life Science Capital Normal University Beijing 100037 China 4. College of Life Science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. College of Grassland Science Gansu Agricultural University Lanzhou 730070 China 6. Department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 23.000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3.5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 3.0 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3.3.pattern using a GeneTAC?G3.arraying robot after being transferred manually into three 3.4-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 3.4-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

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Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica(Phaeophyta)

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Acta Oceanologica Sinica 2012年第6期31卷 139-148页

作者： XUAN Jinsong 1,4,FENG Yanbin 1,WENG Manli 1,ZHAO Ge 1,SHI Jinfeng 1,YAO Jianting 2,WANG Xiuliang 2,GUO Baotai 3.QIAO Lixian 3.DUAN Delin 2,WANG Bin 1 1 The State Key Laboratory of Plant Genomics,institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,china 2 The State Key Laboratory of Marine Biology,institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,china 3.College of Life Sciences,Qingdao Agricultural University,Qingdao 266109,china 4 Department of Biological Science and engineering,school of chemical and Biological engineering,University of Science and technology Beijing,Beijing 100083.china 1. The State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China 4. Department of Biological Science and engineering School of Chemical and Biological Engineering University of Science and Technology Beijing Beijing 100083 China 2. The State Key Laboratory of Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China 3. College of Life Sciences Qingdao Agricultural University Qingdao 266109 China

A high quality cDNA library was constructed from the brown alga Laminaria japonica,with the titer of 1.2×10 5 pfu/*** average insert size of the cDNA library is about 1.6 *** the cDNA library,591 cDNA clones were randomly selected and *** a result,574 EST(expressed sequence tag) sequences were *** of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942625 to *** cDNA library was screened with a α-3. p labeled 453.bp T P S gene probe,which is a partial sequence yielded from Porphyra *** positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of *** TPS cDNA *** PCR amplification,sequencing and assembling,the entire ORF(open reading frame) sequence of the T P S gene was obtained,which was named *** encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 *** LjTPS gene was successfully expressed in *** and *** LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the T P S gene function and mechanism to stress tolerance.

关键词： cDNA library comparative study expressed sequence tag(EST) analysis Laminaria japonica trehalose-6-phosphate synthase(TPS) T P S gene

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Synthesis of N^(11)-anchoring biotinylated artemisinin derivatives and their preliminary biological assessment

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Science china Chemistry 2010年第1期53卷 119-124页

作者： PAN QiYu1,2,WANG ShaoZhong1,LU JinJian3.MENG LingHua3.& YAO ZhuJun1,2 1Nanjing National Laboratory of Microstructures(NNLM),school of Chemistry and chemical engineering,Nanjing University,Nanjing 210093.{3. 2Joint Laboratory of Green Chemistry,Department of Chemistry,University of Science and technology of {3.,Hefei 23.026,{3. 3.tate Key Laboratory of Drug Research,Shanghai institute of Materia Medica,Shanghai institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 201203.{3.. 1. Nanjing National Laboratory of Microstructures (NNLM) School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China2. Joint Laboratory of Green Chemistry Department of Chemistry University of Science and Technology of China Hefei 230026 China3. State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

Unique endoperoxide moiety of artemisinin and its derivatives has been considered the functionality exhibiting highly potent antimalarial and anticancer *** investigate the mechanisms of their biological actions,development of suitable molecular probes including biotinylated derivatives is of extreme *** synthesis and preliminary biological assessment of four new biotinylated artemisinin derivatives have been reported in this work.

关键词： artemisinin endoperoxide biotinylated derivative synthesis anticancer activity

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Microstructure of Bioglass/Hydroxynpatite Coatings on Ti Substrate

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Journal of Materials Science & technology 2001年第1期17卷 35-36页

作者： Congqin NING Qingchang .MENG and Yu ZHOU school of Materials Science and engineering, Harbin institute of {3., Harbin 150001, china Haitao LIU Department of chemical engineering, Qiqihar institute of Light Industry, Qiqihar IG1006. china Sch. of Mater. Sci. Harbin Inst. of Technol. Harbin 150001 China

Composite coatings of bioglass and hydroxyapatite (briefly named HA/BG) with different hydroxyapatite contents on titanium substrate were successfully fabricated. The fabricated coatings are characterized by rough and poriform *** densities of the coatings decrease with the increase of HA content. There is a transition layer with a 5 μm thickness between the BG coating and the substrate. During heat-treatment, hydroxyapatite crystals with hexastyle shape have precipitated from the BG.

关键词： transition layer densities of

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Mechanical and Experimental Study of Sprinkling Water Cooling System on Roof of Shanghai Expo Theme Pavilion

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湖南大学学报（自然科学版） 2009年第S1期36卷 156-159页

作者： ZHAO Hui-zhong1,HUANG Chen1,LI Wei-xiang2,SHI Jin-yue2,TAO Si-yuan1,WANG Qian1,WANG Yu-qing1,ZHANG Min3.1.College of Urban Construction and environment engineering,University of Shanghai for Science and technology,Shanghai 200093.{3. 2.Architectural Design & Research institute of Tongji University,Shanghai 200092,{3. 3.College of Food Science technology,Shanghai Ocean University,Shanghai 2013.6,{3.) College of Urban Construction and environment engineering University of Shanghai for Science and Technology Shanghai 200093 China Architectural Design and Research institute Tongji University Shanghai 200092 China College of Food Science technology Shanghai Ocean University Shanghai 201306 China

Based on the similarity theory,a scale effect model of the spraying water cooling system of Shanghai expo Theme Pavilion's roof was set *** to the typical weather conditions in Shanghai city,different models were analyzed on solar radiation,and different heat transfer amount was *** the following conclusions could be made:At the sunny day of summer in Shanghai,the temperature of inner roof with sprinkling system descended about 3.℃,and the air conditioning load of the whole Theme Pavilion descend more than 3.0 kW.

关键词： Shanghai expo large space atrium solar radiation roof sprinkling system similarity theory prototype

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Orthogonal design for optimization of pigment extraction from surface sediments of the Changjiang River Estuary

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Acta Oceanologica Sinica 2011年第4期30卷 33-42页

作者： ZHAO Jun YAO Peng YU Zhigang Thomas S Bianchi Key Laboratory of Marine environment and Ecology Ministry of Education Ocean University of China Qingdao 266100 China Department of Oceanography Texas A ＆ M University College Station TX 77843 USA Key Laboratory of Marine Chemistry Theory and technology Ministry of Education Ocean University of China Qingdao 266100 China institute of Marine Organic Geochemistry College of Chemistry and Chemical Engineering Ocean University of China Qingdao 266100 China

Using a suitable solvent for extracting pigments from sediment for high performance liquid chromatography（HPLC） analysis is critical for obtaining qualitative and quantitative estimates of phytoplanktonic and benthic algal biomass,as well as community composition. Five methodological factors（sample dehydration,extraction solvent,extraction duration,number of extractions,and ratio of solvent volume：sample weight） were studied using an L 9（3.4 ） orthogonal design in a sedimentary pigment extraction experiment on samples collected from the Changjiang large-river delta-front estuary（LDE）,using HPLC analysis. The results show that the optimal extraction method for sedimentary pigments should include freeze-drying samples prior to extraction. The effects of different factors on sedimentary pigment extraction were separated by the L 9（3.4 ） orthogonal design experiments and showed that the extraction solvent was the most important,with extraction duration the second most important,and numbers of extraction and ratio of solvent volume：sample weight was the least important. The mixed solvent treatment comprised of acetone,methanol and water（80：15：5,by volume） was best for polar pigment extraction,with 100% acetone better for apolar pigments. For most pigments employed in this study（i.e.,peridinin,fucoxanthin,alloxanthin,diatoxanthin,zeaxanthin,pheophytin-a and β-carotene） ,3.h was found to be enough time for extraction from these deltaic sediments. However,for chlorophyll-a,the most important pigment used for estimating algal biomass,12 h was needed. A small amount of solvent（3.ml） with duplicate extractions obtained the greatest amount and diversity of pigments. Unfortunately,no extraction method was found to be suitable for all pigments in sediments. The choice of extraction procedure should be made in accordance with the objective of each study,taking into consideration the properties of sediments and pigments in question.

关键词： sedimentary pigments extraction orthogonal design HPLC Changjiang River Estuary

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Influence of p-layer on the performance of n-i-p μc-Si:H thin film solar cells

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Science china(Physics,Mechanics & Astronomy) 2010年第11期53卷 2042-2046页

作者： YUAN YuJie 1,2,ZHANG KaiLiang 1,2,WEI Zhen 1,2 & GENG XinHua 3.1 College of Electronics Information engineering,Tianjin University of technology,Tianjin 3.03.4,china 2 Tianjin Key Laboratory of Film Electronic and Communication Devices,Tianjin University of technology,Tianjin 3.03.4,china 3.institute of Photo-Electronics Thin Film Devices and Technique,Nankai University,Tianjin 3.0071,china 1. College of Electronics Information engineering Tianjin University of Technology Tianjin 300384 China2. Tianjin Key Laboratory of Film Electronic and Communication Devices Tianjin University of Technology Tianjin 300384 China3. Institute of Photo-Electronics Thin Film Devices and Technique Nankai University Tianjin 300071 China

The high pressure radio frequency plasma enhanced chemical vapor deposition (RF-PECVD) process was adopted to prepare the n-i-p microcrystalline silicon solar cells,the influence of p-type layers on the performance of the solar cells was investigated,and the optimum p layer suited to the n-i-p microcrystalline silicon solar cells was *** experimental results demonstrate that the performance of the solar cells can be highly affected by the structural and optical properties of the p-layers,and the performance of solar cells can be greatly improved by optimizing p *** have achieved an initial active-area efficiency of 8.17% (V oc =0.49 V,J sc =24.9 mA/cm 2 ,FF=67%) for the μc-Si:H single-junction n-i-p solar cells and an initial active-area efficiency of 10.93. (V oc =1.3. V,J sc =13.09 mA/cm 2 ,FF=64%) for the a-Si:H/μc-Si:H tandem n-i-p solar cells.

关键词： microcrystalline silicon solar cells quantum confinement effect p-type layers

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Synthesis of three-dimensional hierarchical cobalt hydroxide microstructures

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Science china Chemistry 2010年第4期53卷 841-845页

作者： SONG XuChun1, ZHAO Yang2, ZHENG YiFan3. YANG E1 & LIU ZhiSheng1 1institute of Physical Chemistry, Department of Chemistry, Fujian Normal University, Fuzhou 3.0007, china 2Department of Chemistry, Henan Normal University, Xinxiang 453.07, china 3.ollege of {3. engineering & Materials Science, Zhejiang University of technology, Hangzhou 3.0014, china 1. institute of Physical Chemistry Department of Chemistry Fujian Normal University Fuzhou 350007 China2. Department of Chemistry Henan Normal University Xinxiang 453007 China3. College of Chemical Engineering & Materials Science Zhejiang University of Technology Hangzhou 310014 China

β-Co(OH)2 with three-dimensional (3.D) structures was prepared by a simple hydrothermal method. It was found that the amount of cetyltrimethylammonium bromide (CTAB), the pH value, and the reaction time all had an important influence on the formation of this morphology. The products were characterized by X-ray diffraction, energy-dispersive X-ray analysis, and scanning electron microscopy. A possible mechanism of the formation of the 3.D microstructures of β-Co(OH)2 was proposed.

关键词： cobalt hydroxide microstructures hydrothermal

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