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Effect of Soil Moisture on Release of Low-Molecular Weight Organic Acids in Root Exudates and the Accu-mulation of Iron in Root Apoplasm of Peanut

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Pedosphere 2000年第4期10卷 333-338页

作者： ZHENG YI and ZHANG FUSUO (Department of Plant Nutrition, china agricultural university, beijing,100094 china) 中国农业大学北京 100094

A three-compartments rhizobox was designed and used to study the low-molecular-weight organic acids in root exudates and the root apoplastic iron of "lime-induced chlorosis" peanut grown on a calcareous soil in relation to different soil moisture conditions. Results showed that chlorosis of peanuts developed under condition of high soil moisture level (250 g kg-1), while peanuts grew well and chlorosis did not develop when soil moisture was managed to a normal level (150 g kg-1). The malic acid, maleic acid and succinic acid contents of chlorotic peanut increased by 108.723. 0.029 and 22.446 ig cm-2, respectively, compared with healthy peanuts. The content of citric acid and fumaric acid also increased in root exudates of chlorotic peanuts. On Days 28 and 42 of peanut growth, the accumulation of root apoplastic iron in chlorotic peanuts was higher than that of healthy peanuts. From Day 28 to Day 42, the mobilization percentages of chlorotic peanuts and healthy peanuts to root apoplastic iron were almost the same, being 52.4% and 52.8%, respectively, indicating that the chlorosis might be caused by the inactivation of iron within peanut plant grown on a calcareous soil under high soil moisture conditions.

关键词： apoplastic iron peanut root exudates soil moisture

Screening of Highly Effective Sinorhizobium meliloti Strains for 'Vector' Alfalfa and Testing of Its Competitive Nodulation Ability in the Field

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Pedosphere 2007年第2期17卷 219-228页

作者： ZENG Zhao-Hai CHEN Wen-Xin HU Yue-Gao SUI Xin-Hua CHEN Dan-Ming College of agronomy and biotechnology China Agricultural University Beijing 100094 （China） College of Biology/Key laboratory of Agro-Microbial Resource and Application Ministry of Agriculture China Agricultural University Beijing 100094 （China）

Seventeen Sinorhizobium meliloti strains from seven provinces in china were used to screen highly effective strains for alfalfa cultivar in a greenhouse study and their symbiotic relationship and competitive ability were studied in the field. CCBAU3.13. was the most effective strain, as evidenced by increase in dry weights. A field experiment showed that the inoculation of alfalfa with CCBAU3.13. resulted in increases of 11.9% and 19.6% of dry matter production and crude protein production, respectively, in forage of monocultured plants. The total dry matter yields of alfalfa and tall fescue in binary culture were increased by 16.3. by inoculation of alfalfa with this strain. These results showed that S. rneliloti strain CCBAU3.13. was an effective inoculant both in the greenhouse and in the field. The analysis of randomly amplified polymorphic DNA （RAPD） by polymerase chain reaction （PCR） from nodule extracts showed that the strain CCBAU3.13. had high competitiveness in the field. It occupied 47.5% of nodules in alfalfa monoculture and 44.4% of nodules in alfalfa-tall fescue binary culture after 20 weeks of growth. In conclusion, a simple system to select highly effective and competitive symbiotic strains specific to alfalfa was established. Using this system, a *** suitable for the alfalfa cultivar ‘Vector＇ grown in Wuqiao County of Hebei Province was obtained.

关键词： alfalfa inoculant selection Sinorhizobium meliloti tall fescue

Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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Science china(Life Sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3.ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3.&ZHAO MaoLin 1 1 beijing Agro-biotechnology Research Center,beijing Academy of Agriculture and Forestry Science,beijing 100097,china 2 Department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,china 3.College of Life Science,Capital Normal university,beijing 100037,china 4 College of Life Science,Zhongkai university of Agriculture and Technology,Guangzhou 510225,china 5 College of Grassland Science,Gansu agricultural university,Lanzhou 73.070,china 6 Department of Biology,Henan Institute of Science and Technology,Xinxiang 453.03.china 1. beijing Agro-biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan 411104 China 3. College of Life Science Capital Normal University Beijing 100037 China 4. College of Life Science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. College of Grassland Science Gansu Agricultural University Lanzhou 730070 China 6. Department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 23.000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3.5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 3.0 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3.3.pattern using a GeneTAC?G3.arraying robot after being transferred manually into three 3.4-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 3.4-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

Infection of Wolbachia in Trichogramma cacoeciae

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agricultural Sciences in china 2003年第6期2卷 634-638页

作者： WANG Wei-hua, GONG Peng and SHEN Zuo-rui(College of agronomy and biotechnology , china agricultural university, {3. 100094 , P. R. china) College of agronomy and biotechnology China Agricultural University Beijing 100094 P. R. China

Wolbachia is a symbiotic bacterium that alters reproductive behavior of numerous arthropods. It is the most advanced way to study phytogeny and classification based on sequences of the wsp gene. The presence of Wolbachia in a lab strain of the thelytokous Trichogramma cacoeciae was firstly identified based on amplification and sequencing part of the wsp gene. Aligned the resulting sequence with the published ones, the phylogenetic relationships among Wolbachia which was found in T. cacoeciae, T. dendrolimi and other insects were established. The Wolbachia that found in T. cacoeciae and in T. dendrolimi belong to the Kue group and the Pip group, respectively.

关键词： Wolbachia Trichogramma wsp gene Phylogeny

Seasonal influence on the development of parthenotes and cloned embryos in pigs

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Progress in Natural Science:Materials International 2007年第10期17卷 1172-1176页

作者： Zhang Kun1, Zhang Yunhai3.4, Pan Dengke5, Ma Yufang1, Wei Hengxi1, Zhang Jian6, Li Xuyang7, Wang Shaohua1, Li Yan2, Dai Yunping1,2 and Li Ning1(1. State Key laboratory for Agrobiotechnology, College of Biological Science, china agricultural university, {3. 100094, china 2. JiPulin Biotech Ltd., {3. 100094, china 3. Danish Institute of agricultural Sciences, 883. Tjele, Denmark 4. College of Animal Science and Technology, Anhui agricultural university, Hefei 23.03., china 5. Institute of Animal Sciences, Chinese Academy of agricultural Sciences, {3. 100094, china 6. Animal Clinical Hospital, china agricultural university, {3. 100094, china 7. Hebei Minghui Group of Pigs Breeding, Sanhe 065200, china) State Key laboratory for Agrobiotechnology College of Biological Science China Agricultural University Beijing 100094 China JiPulin Biotech Ltd. Beijing 100094 China Danish Institute of agricultural Sciences 8830 Tjele Denmark College of Animal Science and Technology Anhui Agricultural University Hefei 230036 China Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing 100094 China Animal Clinical Hospital China Agricultural University Beijing 100094 China 7. Hebei Minghui Group of Pigs Breeding Sanhe 065200 China

Seasonal influence on the developmental ability of porcine embryos was studied by comparisons of the number of cumulus-oocytes-complexes (COCs) per ovary pair and meiotic maturation ability of oocytes in different seasons; and by the observations on developmental competence of the parthenogenetic oocytes and cloned embryos collected from different seasons. We found that the number of COCs per ovary pairs was significantly higher in spring compared to the other seasons (P<0.05). However, no significant difference was found in the rate of oocytes progressing to MⅡ in four seasons (P<0.05). In addition, the rate of blastocyst formation of parthenogenetic embryos in summer was obviously declined compared to the other seasons (P<0.05), and an increased blastocyst rate of cloned porcine embryos was found in spring compared to autumn and winter (P<0.05). The results suggest that there should be a seasonal influence on the developmental competence of porcine embryos.

关键词： pig cloned parthenogenetic season

Phototoxic effect of UVR on wild type, ebony and yellow mutants of Drosophila melanogaster: Life Span, fertility, courtship and biochemical aspects

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Science china(Life Sciences) 2008年第10期51卷 885-893页

作者： WANG ZhePeng1,2, LIU RuiFang2, WANG AnRu3. DU LiLi1 & DENG XueMei1 1 College of Animal Science and Technology, china agricultural university, beijing 100094, china 2 Vocational and Technologic College, Inner Mongolia Agriculture {3., Baotou 014109, china 3.College of Veterinary Medicine, china agricultural university, beijing 100094, china 1. College of Animal Science and Technology China Agricultural University Beijing 100094 China 2. Vocational and Technologic College Inner Mongolia Agriculture University Baotou 014109 China 3. College of Veterinary Medicine China Agricultural University Beijing 100094 China

Melanin plays an important role in protecting organisms from ultraviolet radiation (UVR). Therefore, it is possible that differently colored strains can show different sensitivities to UVR. In the present work, life span, fertility and courtship behavior of wild type (w), ebony (e) and yellow (y) strains of Drosophila melanogaster were studied to evaluate their sensitivity to ultraviolet (UV). Because a range of photo- toxic effects of UVR are mediated through generation of free radicals, levels of free radicals, lipid per- oxide (malondialdehyde, MDA) and superoxide dismutase (SOD) activity of three strains were examined to indicate their antioxidant defending ability and oxidative status. It was shown that w always had the highest lifespan and fertility not only in the control but also in UV-exposed groups. Moreover, lifespan and fertility of e were significantly higher (P0.05). MDA levels were increased in the UV dose-dependent manner (P=0.0495). In con- clusion, our results suggested that UVR can decrease life span and fertility of flies and do harm to courtship, which may be due to oxidative damage to flies tissues (e.g. central nervous system) induced by free radicals. w had the highest tolerance to UVR, which may be ascribed to its advantage of survival under the natural condition and at high level of SOD activity. Then differences of pigment between e and y in absorbing UV, shielding agai

关键词： ultraviolet radiation Drosophila melanogaste free radicals pigment

Types of voltage-dependent calcium channels involved in high potassium depolarization-induced amylase secretion in the exocrine pancreatic tumour cell line AR4-2J

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Cell Research 1998年第1期8卷 23-31页

作者： CUI ZONG JIE (beijing agricultural university Faculty of Biological Sciences beijing 100094, china) beijing agricultural university Faculty of Biological Sciences Beijing 100094 China

In the perifused fura-2 loaded exocrine pancreatic acinar cell line AR4-2J pulses of high potassium induced repetitive increases in intracellular calcium. Attached cells when stimulated with high potassium secreted large amount of amylase. High potassium-induced secretion was dependent both on the concentration of potassium and duration of stimulation. High potassium induced increases in intracellular calcium were inhibited by voltage-dependent calcium channel antagonists with an order of potency as follows: nifedipine > ω-agatoxin IVA > ω-conotoxin GVIA. In contrast, the L-type calcium channel antagonist nifedipine almost completely inhibited potassium-induced amylase secretion, whereas the N-type channel antagonist ω-conotoxin GVIA was without effect. The P-type channel antagonist ω-agatoxin IVA had a small inhibitory effect, but this inhibition was not significant at the level of amylase secretion. In conclusion, the AR4-2J cell line possesses different voltage-dependent calcium channels (L, P,N) with the L-type predominantly involved in depolarization induced amylase secretion.

关键词： AR4-2J pancreatic acinar cells amylase secretion calcium channels

Overall Planning of Development of Urban-Rural Areas:A Policy Mechanism for Urban-Rural Coordinated Growth

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Chinese Journal of Population,Resources and Environment 2004年第1期2卷 57-60页

作者： Sun JinSociology Department, china agricultural university, beijing 100874, china Sociology Department China Agricultural University Beijing 100874 China

While analyzing overall planning and coordinated development of urban-rural areas, this paper shows that overall planning for the development of urban-rural areas is a requirement for the best use of productive forces. It means the setting up of a policy mechanism for coordinated growth. Recognizing this, the government should set up a way to lead and manage a unified program to increase service and decrease controlling administration power, while safeguarding and fulfilling the farmers' ownership to the means of production.

关键词： Overall planning of development of urban-rural areas Cxjordinated development of urban-rural areas

Hydrogen metabolic pathways of Rhodospirillum rubrum under artificial illumination

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Chinese Science Bulletin 2010年第1期55卷 32-37页

作者： ZHU RuiYan1,2,3.& LI JiLun1 1 College of Biological Sciences, State Key laboratory for Agrobiotechnology, china agricultural university, {3. 100193. china 2 College of Environmental and Chemical Engineering, Yanshan university, Qinhuangdao 066004, china 3.Engineering Research Center for Bioenergy of Hebei Province, Qinhuangdao 066004, china College of Biological Sciences State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100193 China College of Environmental and Chemical Engineering Yanshan University Qinhuangdao 066004 China Engineering Research Center for Bioenergy of Hebei Province Qinhuangdao 066004 China

Multiple enzymes involved in hydrogen metabolism have been detected in photosynthetic bacterium Rhodospirillum rubrum un-der various growth conditions. To investigate hydrogen metabolic pathways and the contribution of each pathway to hydrogen photoproduction in R. rubrum under the artificial illumination condition, three mutants were constructed, including nifHanfDG double mutant lacking both Fe-nitrogenase and Mo-nitrogenase, anfDG mutant lacking Fe-nitrogenase and nifHhupL double mu-tant (uptake hydrogenase deficient mutant). Comparison of the hydrogen production of these 3.mutants with R. rubrum wild type and the uptake hydrogenase deficient mutant showed that there was a third pathway involved in hydrogen production besides Mo-nitrogenase and Fe-nitrogenase, which mainly contributed to hydrogen evolution. Only a small portion of hydrogen was gen-erated by the third pathway. The hydrogen produced by Mo-nitrogenase, Fe-nitrogenase and the third pathway was about 93.5%, 4.9% and 1.5%, respectively, while the hydrogen consumed by uptake hydrogenase was about 13.3.. The investigation of the formate-linked hydrogenase activity indicated that the third pathway for hydrogen production was not mediated by the for-mate-linked hydrogenase, but probably by some unknown enzyme.

关键词：氢代谢人工照明代谢途径红螺菌固氮酶脱氢酶突变体体吸收