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Mass Spectrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of A Novel Endolytic Heparinase

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 840-844页

作者： Yapeng CHAO1,3. Shaoxiang XIONG2. Xiulan CHENG1, and Shijun QIAN1* 1Institute of Microbiology, chinese {2. of Sciences, beijing 100080,{3. 2.enter of Mass Spectrometry, Institute of Chemistry, chinese {2. of Sciences, beijing 100080,{3. 3.8} School of the chinese {2. of Sciences, beijing 10003.,{3. Institute of Microbiology Chinese Academy of SciencesBeijing 100080 China graduate School of the chinese {2. of Sciences{3. 10003. China Center of Mass Spectrometry Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was de- graded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccha- ride components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 3.9 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak III represented an N- acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate- substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin.

关键词： electrospray ionization-mass spectrometry heparin disaccharides heparinase high perfor- mance liquid chromatography Sphingobacterium sp.

The role of aerial algae in the formation of the landscape of the Yunnan Stone Forest,Yunnan Province, china

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Science china Earth Sciences 2004年第9期47卷 846-864页

作者： TIAN Youping1,3. ZHANG Jie2. SONG Linhua1 & BAO Haosheng2.1. Institute of Geographic Sciences and Natural Resources Research, chinese {2. of Sciences, beijing 100101,{3. 2. Department of Geography, Nanjing university, Nanjing 2.0093.{3. 3. School of Earth Sciences and Resources,{3. university of Geosciences, beijing 100083.{3. Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China Department of Geography Nanjing University Nanjing 210093 China School of Earth Sciences and Resources China University of Geosciences Beijing 100083 China

Aerial algae on the surface of carbonate rocks at the Stone Forest, Shilin County, Yunnan Province, china, and their bioerosion were investigated in the field and studied in the laboratory in detail. Through the observation, identification and statistics of more than one hun- dred algal samples and rock samples with the optical microscopes (stereomicroscope, biological microscope) and the scanning electronic microscope (SEM), the relationships between erosional forms on the surface of the Stone Forest and algae and/or algal communities and the genetic mechanism for the formation of erosional forms were analyzed. It is suggested that aerial algae play an active role in bioerosive processes that may affect the formation of karst erosional forms. These effects include both direct and indirect ones. The direct effect is the initiative control (algal shape-controlling role) of algae on the formation of karst forms of various scales, mostly mi- cro-scale (3.) and minor-scale (10-3.10-1m) erosional forms. The algal shape-controlling roles can be divided into the algal individual shape-controlling role and the algal community shape-controlling role. The former mostly controls the formation of micro-scale erosional forms, while the latter mostly controls the formation of micro-scale and smaller minor-scale erosional forms. The indirect effect refers to the promoting role of algae in the formation of karst forms, which may affect the formation of karst forms of all types and scales. The bioerosion of algae accelerates the weathering process of the whole Stone Forest karst landforms.

关键词： aerial algae, bioerosion, the Stone Forest, Yunnan Province, china.

An efficient chiral synthesis of (R)-N-[3.acetyl-4-(2.hydroxy-3.isopropylamino-propoxy)phenyl]-butanamide with high enantioselectivity

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Science china Chemistry 2009年第8期52卷 1216-1219页

作者： WANG NaiXing1,2.& TANG XinLiang1,3.1 Technical Institute of Physics and Chemistry,chinese {2. of Sciences,beijing 100080,{3. 2.College of Chemistry and Chemical Engineering,graduate university of chinese {2. of Sciences,beijing 10004.,{3. 3.School of Chemical Engineering and Environment,beijing Institute of Technology,beijing 100081,{3. 1. Technical Institute of Physics and Chemistry Chinese Academy of Sciences Beijing 100080 China 2. College of Chemistry and Chemical Engineering Graduate University of Chinese Academy of Sciences Beijing 100049 China 3. School of Chemical Engineering and Environment Beijing Institute of Technology Beijing 100081 China

R-Enantiomer of the β-receptor antagonist N-[3.acetyl-4-(2.hydroxy-3.isopropylamino-propoxy)phenyl] butanamide with high enantioselectivity was synthesized from cheap starting materials and enantiopure chiral reagent through an efficient,convenient and practical synthetic *** product was detected by 1H NMR,13. NMR,and MS,and the enatiomeric excess was determined by chiral HPLC analysis using a chiracel AD-H column.

关键词： chiral synthesis enantioselectivity enatiomeric excess chiral HPLC analysis

Mechanism and Defense on Malicious Code

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Wuhan university Journal of Natural Sciences 2005年第1期10卷 83-88页

作者： WEN Wei-ping 1,2.3. QING Si-han 1,2.3.1. Institute of Software, the {2. academy of Sciences, beijing 100080,{3. 2.Engineering Research Center for Information Security Technology, the {2. academy of Sciences, beijing 100080,{3. 3.graduate School of the {2. academy of Sciences, beijing 100080,{3. Institute of Software the Chinese Academy of Sciences Beijing 100080 China

With the explosive growth of network applications, the threat of the malicious code against network security becomes increasingly serious. In this paper we explore the mechanism of the malicious code by giving an attack model of the malicious code, and discuss the critical techniques of implementation and prevention against the malicious code. The remaining problems and emerging trends in this area are also addressed in the paper.

关键词： malicious code attacking model mechanism defense system security network security

A trust region algorithm for bilevel programing problems

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chinese Science Bulletin 1998年第10期43卷 820-824页

作者： LIU Guoshan 1, HAN Jiye 2.and WANG Shouyang 3.1. Department of Mathematics, beijing Normal {2., beijing 100875,{3. 2. Institute of Applied Mathematics, chinese academy of Sciences, beijing 100080,{3. 3. Institute of Systems Science, Chi Department of Mathematics Beijing Normal University Beijing China Institute of Applied Mathematics Chinese Academy of Sciences Beijing China Institute of Systems Science Chinese Academy of Sciences Beijing China

A trust region algorithm is proposed for solving bilevel programming problems where the lower level programming problem is a strongly convex programming problem with linear constraints. This algorithm is based on a tr... 详细信息

关键词： bilevel programming problem trust region algorithm global convergence.

Effective solutions to a global 3. visual system in networking environments

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Science china Earth Sciences 2005年第11期48卷 2032-2039页

作者： ZHANG Liqiang1,2. ZHANG Yan3. YANG Chongjun2. LIU Suhong1, REN Yingchao2. RUI Xiaoping2.& LIU Donglin2.1. State Key Laboratory of Remote Sensing Sciences, School of Geography and Remote Sensing, beijing Normal {2., beijing 100875,{3. 2. State Key Laboratory of Remote Sensing Sciences, Institute of Remote Sensing Applications, chinese academy of Sciences, beijing 101001,{3. 3. Institute of Geographical Sciences and Natural Resources Research, chinese academy of Sciences, beijing 101001,{3. 1. State Key Laboratory of Remote Sensing Sciences School of Geography and Remote Sensing Beijing Normal University 100875 Beijing China 2. chinese academy of Sciences State Key Laboratory of Remote Sensing SciencesInstitute of Remote Sensing Applications 101001 Beijing China 3. Institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences 101001 Beijing China

The development of Web-based global 3. visual system has made progresses. However, there exist few mature and effective ways for managing, transmitting and visualizing massive spatial data. Based on the related work, the paper illustrates the ellipsoidal quadtree technique for rapid access multi-scale and multiple level geographical data, integrates the streaming with level-of-detail rendering method for transmitting the data on the network, and im-plements large-scale terrain surface simplification using M-band wavelet transforms and mul-tiresolution triangulations. We fulfil a web-based global terrain visual system using COM com-ponents on the basis of the above techniques. The system has a good prospect in the military simulation and city plans.

关键词： massive spatial data, streaming transmission, system architecture, M-band wavelet

Explicit analytical solutions of the anisotropic Brinkman model for the natural convection in porous media(Ⅱ)

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Science china(Physics,Mechanics & Astronomy) 2005年第4期48卷 422-430页

作者： CAI Ruixian1, GOU Chenhua1, 2.& ZHANG Na1 1. Institute of Engineering Thermophysics, chinese academy of Sciences, {2. 100080,{3. 2. graduate School, chinese academy of Sciences, {2. 100080,{3. 1. Institute of Engineering Thermophysics Chinese Academy of Sciences 100080 Beijing China 2. graduate School Chinese Academy of Sciences 10080 Beijing China

Some algebraically explicit analytical solutions are derived for the anisotropic Brinkman model―an improved Darcy model―describing the natural convection in porous media. Besides their important theoretical meaning (for example, in analyzing the non-Darcy and anisotropic effects on the convection), such analytical solutions can be the benchmark solutions that can promote the development of computational heat and mass transfer. Some solutions considering the anisotropic effect of permeability have been given previously by the authors, and this paper gives solutions including the anisotropic effect of thermal conductivity and the effect of heat sources.

关键词： explicit analytical solution, porous medium, natural convection, Brinkman model, anisotropic.

Modeling of elastic wave propagation on a curved free surface using an improved finite-difference algorithm

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Science china(Physics,Mechanics & Astronomy) 2004年第5期47卷 633-648页

作者： WANG Xiuming1,3.& ZHANG Hailan2.1. CSIRO Petroleum, ARRC, PO BOX 113., Technology Park, Bentley, WA 6102. Australia 2. Institute of Acoustics, {2. academy of Sciences, beijing 100080,{3. 3. Present address: Institute of Acoustics, {2. academy of Sciences, beijing 100080,{3. 1. CSIRO Petroleum ARRC Technology Park PO BOX 1130 6102 Bentley WA Australia 2. Institute of Acoustics Chinese Academy of Sciences 100080 Beijing China

Based on existing direct and imaging methods of a staggered finite-difference scheme, an improved algorithm for staggered finite-difference is proposed to implement rugged topographic free boundary conditions. This method assumes that the free surface can be implemented with horizontal and vertical free surface segments and their corners; the free surface passes through the grid points of shear stress components, instead of the normal stress components. Imaging is carried out for stress components in both horizontal and vertical directions, thus increasing the accuracy. To update particle- velocities, imaging and updating are first performed in the horizontal direction, and then in the vertical direction. The numerical results for elastic flat horizontal free surface with the imaging method and those for flat free surfaces of various slope angles with the proposed method are compared, and are shown to be in good agreement. The advantage of the proposed method is that only the stresses are dealt with in implementing the free surface into the staggered algorithm, which improves computation efficiency.

关键词： elastic wave simulation, curved free surface, staggered finite-difference, algorithm stability.

Eclogites in the interior of the Tibetan Plateau and their geodynamic implications

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chinese Science Bulletin 2009年第15期54卷 2556-2567页

作者： ZHANG KaiJun1,2.& TANG XianChun2.3.1 Department of Earth Sciences, Nanjing university, Nanjing 2.0093. china 2.Guangzhou Institute of Geochemistry, chinese {3. of Sciences, Guangzhou 510640, china 3.graduate university of chinese {3. of Sciences,{12., china Department of Earth Sciences Nanjing University Nanjing 210093 China Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China graduate university of {2. {3. of Sciences Beijing 100049 China

Eclogites have been recently reported in the interior of the Tibetan Plateau, including in the central Qiangtang metamorphic belt, in the Basu metamorphic massif of the eastern Bangong-Nujiang suture zone, and at Songdo and Pengco in the eastern Lhasa terrane. Some typical ultrahigh-pressure (UHP) metamorphic phenomena, e.g., garnet exsolution from clinopyroxene, were documented in the Basu and Pengco eclogites. The UHP metamorphism in the interior of the Tibetan Plateau marked by these eclogites generally took place in the Early Mesozoic. Along with exhumation of these eclogites, (post-) collision-related magmatism extensively occurred around the central Qiangtang belt, the eastern Ban-gong-Nujiang suture zone, and the eastern Lhasa terrane. The occurrence of these Early Mesozoic ec-logites manifests an out-of-sequence evolution of the Tethys, and they could be a product of diachro-nous collision between the eastern Qiangtang terrane and the irregular continental margin of the united western Qiangtang-Lhasa plate, along the linked eastern Bangong-Nujiang-central Qiangtang zone. The collision-related magmatic rocks could have been originated from lithospheric thickening, melting, or detachment due to the collision. The presence of UHP metamorphic rocks in central Qiangtang and Basu implies likely continental deep-subduction, and the denudation of these two metamorphic zones could have served as the source of the Triassic turbidites in the Songpan-Garzê complex and the Ju-rassic turbidites in the western Bangong-Nujiang zone, respectively. However, studies of the eclogites in the interior of the Tibetan Plateau just began, and many principal aspects still remain to be explored, such as their distributions, typical lithologies and minerals, temperature-pressure conditions, timing of formation and exhumation, protoliths and tectonic setting, and relationship with the evolution of the Tethys and large-scale basins in Tibet.

关键词：地球动力学意义青藏高原榴辉岩