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Insulin-sensitizing effects of a novel α-methyl-α-phenoxylpropionate derivative in vitro

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Acta Pharmacologica Sinica 2007年第3期28卷 417-422页

作者： Hao-shu WU~(2.4) Juan-hong YU~(3.4) Ying-yin LI~2.Yu-she YANG~3.Qiao-jun HE~2.Yi-jia LOU~(2.5) Ru-yun JI~3.~2.epartment of Pharmacology and Toxicology,College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 3.0058,china ~3.tate Key Laboratory of Drug research,{2. Institute of Materia Medica,{2. Institutes for Biological Sciences,Graduate School of the Chinese Academy of Sciences,{2. 2.12.3.china Department of Pharmacology and Toxicology College of Pharmaceutical Sciences Zhejiang University Hangzhou China Department of State Key Laboratory of Drug research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Graduate School of the Chinese Academy of Sciences Shanghai China

Aim:To examine the insulin sensitizing effects of a novel α-methyl-α-phenoxylpropionate derivative YY2. in insulin-sensitive cell ***:Theperoxisome proliferator-activated receptor γ（PPARγ）agonist bioactivities of YY2.were detected by a preadipocyte differentiation ***-PCR and Westernblotting analysis were used to detect the expression of the target gene or *** effects of YY2. on insulin-mediated glucose consumption were determined inthe HepG2.human hepatocellular carcinoma ***:YY2. could enhancethe differentiation of preadipocytes to adipocytes and upregulate the gene ex-pression of PPARγ2.as well as the protein expression of insulin receptor sub-strate-1（IRS-1）,glucose transporter-4（GLUT4）,and adiponectin（ACRP3.）.Theeffects on GLUT4 and ACRP3. could be reversed by the PPARγ inhibitor ***,YY2. efficiently reduced glucose consumptions in HepG2.cells after2. h culture,and the effects were related to insulin and YY2. ***:YY2.,a potential insulin-sensitizing agent like rosiglitazone,couldenhance glucose consumption in HepG2.cells in a concentration-and insulin-dependent *** may improve the insulin resistance associated with type 2.iabetes.

关键词： YY2. peroxisome proliferator-activated receptor y insulin sensitivity glucose consumption

Effect of heat-treatment on the surface properties of gallium phosphide nanosolids by Raman spectroscopy

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Rare Metals 2006年第3期25卷 253-259页

作者： ZHANG Zhaochun YUE Longyi GUO Jingkang School of Materials Science and Technology Shanghai University Shanghai 200072 China Bioinformatics research center Shanghai University Shanghai 200072 China

Raman spectra of gallium phosphide （GAP） nanosolids （unheated and heat-treated at 598 and 72.0}K, respectively） were investigated. It was observed that both the longitudinal optical mode （LO） and the transverse optical mode （TO） displayed an asymmetry on the low-wavenumber side. The scattering bands were fitted to a sum of four Lorentzians which were assigned to the LO mode, surface phonon mode, TO mode, and a combination of Ga-O-P symmetric bending and sum band formed from the X-point TA ＋ LA phonons, respectively. Analysis of the characteristic of surface phonon mode revealed that the surface phonon peak of the GaP nanosolids could be confirmed. In the infrared spectrum of the GaP nanoparticles, we observed the bands on account of symmetric stretching and bending of PO2. as well as stretching of Ga-O The Raman scattering intensity arising from the Ga-O-P linkages increased as increasing the heat-treatment temperature.

关键词： semiconductor materials nanosolids Raman spectrum gallium phosphide

Biological networks to the analysis of microarray data

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Progress in Natural Science:Materials International 2006年第12期16卷 1242-1251页

作者： FANG Zhuo~1, LUO Qingming~1**, ZHANG Guoqing~1 and LI Yixue~2.*(1. Hubei Bioinformatics and Molecular Imaging Key Laboratory,Huazhong University of Science and Technology, Wuhan 43.074, china 2. {2. {2. for Bioinformation and Technology, {2. 2.02.0}, china) Hubei Bioinformatics and Molecular Imaging Key Laboratory Huazhong University of Science and Technology Wuhan 430074 China shanghai center for Bioinformation and Technology Shanghai 200235 China

Microarray technology, which permits rapid and large-scale screening for patterns of gene expressions, usually generates a large amount of data. How to mine the biological meanings under these data is one of the main challenges in bioinformatics. Compared to the pure mathematical techniques, those methods incorporated with some prior biological knowledge generally bring better interpretations. Recently, a new analysis, in which the knowledge of biological networks such as metabolic network and protein interaction network is introduced, is widely applied to microarray data analysis. The microarray data analysis based on biological networks contains two main research aspects: identification of active components in biological networks and assessment of gene sets significance. In this paper, we briefly review the progress of these two categories of analyses, especially some representative methods.

关键词： biological networks microarray data analysis subnetwork gene set.

TNF receptor-associated factor-2.binding site is involved in TNFR75-dependent enhancement of TNFR55-induced cell death

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Cell research 2001年第3期11卷 217-222页

作者： LU FANG, JIA FANG, CHANG QING CHEN shanghai research {2. of Biotechnology, shanghai Institutes for Biological Sciences the Chinese Academy of Sciences, shanghai 2.02.5}, china shanghai research {2. of Biotechnology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai China

TNF recepter-55 is the main mediator of TNF-induced apoptosis. TNF receptor-75-dependent induction or enhancement of cytotoxicity has been explained by intracellular signaling, 'ligand passing' , or induction of endogenous TNF. To study the function of human TNF receptor-75 (hTR75) and the interaction between human TNF receptor-55 (hTR55) and hTR75 in hTNFa-induced cytotoxicity, HEp-2.cells were transfected with bicistronic expression vector of hTR75 and its deletion mutants genes. hTNFa-induced cytotoxicity was determined by crystal violet colorimetric method. The expression of hTR75 and its deletion mutants in HEp-2.cells was demonstrated by RT-PCR and indirect ELISA. We found that the overexpressed hTR75 could significantly increase the susceptibility of HEp-2.cells to hTNFa which especially required TRAF2.binding site. hTR75 could not only mediate partial hTNFa-induced cytotoxicity independently but also fulfill an accessory role in enhancing or synergizing hTR55-mediated cytotoxicity

关键词： hTNFa hTNF receptors TRAF2 cytotoxcity signal transduction.

Effect of GNTI,a kappa opioid receptor antagonist,on MK-801-induced hyperlocomotion and stereotypy in mice

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Acta Pharmacologica Sinica 2006年第11期27卷 1401-1408页

作者： Chun-ting QI~2.Hong ZOU~2.Chen-hao ZHANG~2.Qing-lian XIE~2.Mei-lei JIN~(2.4)Lei YU~(3.4)2.research {2. for Biotechnology,{2. Institutes for Biological Sciences,Chinese Academy of Sciences,{2. 2.02.0},china 3.Department of Genetics and {2. of Alcohol Studies,Rutgers,the State University of New Jersey 08854-8001,USA research center for Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Department of Genetics and center of Alcohol Studies Rutgers the State University of New Jersey USA

Aim:To examine the effect of GNTI [5′-guanidinyl-17-(cyclopropylmethyl)-6,7-dehydro-4,5α-epoxy-3.14-dihydroxy-6,7-2.,3.-indolomorphinan],a selective antago-nist for the kappa opioid receptor,in the MK-801(dizocilpine maleate)-inducedbehavioral model of psychosis in schizophrenia as a way to explore the involve-ment of the kappa opioid receptor in modulating psychotic symptoms ***:Two doses of MK-801(0.3.mg/kg and 0.6 mg/kg)wereadministered by systemic injection in mice to induce psychosis-like behavior as arodent schizophrenia model,preceded by an injection of different doses of *** locomotion and stereotypy were measured as the behavioral endpoints forquantitative ***:GNTI inhibited MK-801-induced hyperlocomotionand *** particular,GNTI showed differential modulation of stereotypyinduced by 0.3.mg/kg vs 0.6 mg/kg ***:Antagonism of kappaopioid receptors attenuates MK-801-induced behavior,suggesting a potentialinvolvement of the kappa opioid receptor in psychosis-like symptoms *** appears to be a useful pharmacological tool to explore thekappa opioid receptor function in vivo.

关键词： GNTI kappa opioid receptor MK-801 schizophrenia model

Identification and Analysis of Genes Present in Leptospira interrogans serovar lai but Absent in L. biflexa serovar monvalerio

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 832-839页

作者： Ping HE, Xiang-Yan ZHANG, Xiao-Kui GUO*, Bao-Yu HU, Xiao-Tian HUANG, Yang YANG, and Guo-Ping ZHAO1,2. Department of Microbiology and Parasitology, shanghai Second Medical University, shanghai 2.002.,{2. 1 {3. center of Biotechnology, Institutes for Biological Sciences, Chinese Academy of Sciences, shanghai 2.003.,{2. 2.hinese National Human Genome center at shanghai, Zhangjiang High Tech Park, shanghai 2.12.3.{2. Department of Microbiology and Parasitology Shanghai Second Medical UniversityShanghai 200025 China research center of Biotechnology Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Chinese National Human Genome center at shanghaiZhangjiang High Tech Park Shanghai 201203 China

Genes present in virulent bacterial strains but absent in avirulent close relatives can be of great biologic and clinical interest. This project aimed to identify strain specific DNA sequences of Leptospira interrogans serovar lai, which is absent in the saprophytic L. biflexa serovar monvalerio, via suppression subtractive hybridization with the former as the tester while the latter as the driver. The mixture of PCR amplified DNA fragments from two subtractive hybridization experiments were cloned into pMD18-T vector and the positive clones were identified by dot blotting against the chromosome DNA of the two strains individually. After DNA sequencing and analysis, the distribution of these genomic fragment sequences in a panel of pathogenic and nonpathogenic leptospires was investigated employing dot blot analysis. Among the 188 positive clones randomly chosen, 2. contained the tester strain specific genomic regions, of which, 5 were non-coding fragments while the others contained 2.0}distinct protein coding sequences. Besides 9 genes encoding functional proteins, 12.genes encode unknown proteins and the rest two genes encode proteins with recognizable domain structures, one for a putative leucine-rich repeats (LRR) family protein while the other as an outer-membrane protein. Our experiment results indicated that suppression subtractive hybridiza- tion is effective for screening specific DNA sequences between two leptospiral strains, and some of these sequences might be responsible for virulence determination. Further analysis of these DNA sequences will provide important information on the pathogenesis of Leptospira.

关键词： Leptospira interrogans serovar lai suppression subtractive hybridization (SSH) specific genes

Forcing mechanism of the Pleistocene east Asian monsoon variations in a phase perspective

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Science china Earth Sciences 2005年第10期48卷 1708-1717页

作者： TIAN Jun1,2. WANG Pinxian1, CHENG Xinrong1, WANG Rujian1 & SUN Xiangjun1,3.1. State Key Laboratory of Marine Geology, Tongji University, shanghai 2.0092.{2. 2. Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 10002.,{2. 3. Institute of Botany, Chinese Academy of Sciences, Beijing 100093.{2. Tongji University Chinese Academy of Sciences

The deep sea records from the ODP Sites 1143.and 1144 in the northern and southern South china Sea (SCS), including foraminiferal δ 18O and δ 13., Opal% and pollen percentage, reveal that the variations of the east Asian monsoon have been closely correlated with the variations of the Earth’s orbital parameters (eccentricity, obliquity and precession) and the global ice volume on orbital scale. All the monsoonal proxies show strong 100 ka, 41 ka and 2.0}ka cycles. Although G. ruber δ 13. of Site 1143.is coherent with the ETP (ETP= normalized (eccentricity + obliquity-precession) at eccentricity, obliquity and precession bands, most of the coherent relationship focuses on the precession band, and the other monsoonal proxies are coherent with the ETP only at the precession band, which indicate that precession dominates the Pleistocene tropical climate changes. The phase relationship of the monsoonal proxies with the foraminiferal δ 18O implies that the global ice volume changes have played a significant role in modulating the east Asian monsoon climate, at least dominating the winter monsoon. This forcing mechanism of the east Asian monsoon is apparently different from that of the Indian ocean mon-soon. The variations of the east Asian monsoon at the precession band, at least that of the winter monsoon, have been controlled not only by the sensible heating but also by the latent heating of the surface water in the South china Sea.

关键词： South china Sea, ODP, east Asian monsoon, orbital forcing, global ice volume.

Nanocrystallization of Cementite in 0.4C-1Cr Steel During High-Power Surface Processing

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Journal of Wuhan University of Technology(Materials Science) 2005年第3期20卷 60-63页

作者：赵新奇 School of Materials Science and Engineering Tongji University shanghai Baosteel research Institute Shanghai 201900China

The microstructures of the nanocrystalline surface layer of a quenched and high temperature tempered 0. 4C- 1Cr steel induced by high-power surface processing （HPSP） technique were characterized by scan- ning eleetron microscopy and transmission electron microscopy. The results indicate that a nanocrystalline layer was fabricated on the surface of the steel 19 using HPSP treatment. The mean grain size in the surface layer is about 11 nm. The nanocrystallization of cementite is prior to that of the matrix phase, ferrite.

关键词： cementite high-power surface processing surface nanocrystallization

Large-eddy simulations and vortex structures of turbulent jets in crossflow

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Science china(Physics,Mechanics & astronomy) 2007年第1期50卷 118-132页

作者： GUAN Hui1,2.& WU ChuiJie2.1 School of Mechanical and Power Engineering,East china University of Science and Technology,shanghai 2.02.2.,{2. 2.{{2. center for Fluid Dynamics,PLA University of Science and Technology,Nanjing 2.1101,{2. School of Mechanical and Power Engineering East China University of Science and Technology Shanghai China research center for Fluid Dynamics PLA University of Science and Technology Nanjing China

Using the method of large-eddy simulation, the 3.dimensional turbulent jets in crossflow with stream-wise and transverse arrangements of nozzle are simulated, emphasizing on the dynamical process of generation and evolution of vortex structures in these flows. The results show that the basic vortex structures in literatures, such as the counter-rotating vortex pair, leading-edge vortices, lee-side vortices, hanging vortices, kidney vortices and anti-kidney vortices, are not independent physical substances, but local structures of the basic vortex structure of turbulent jets in crossflow-the 3.D stretching vortex rings originating from the orifice of the nozzle, which is discovered in this study. Therefore, the most important large-scale structures of turbulent jets in crossflow are unified to the 3.D vortex rings which stretch and twist in stream-wise and swing in transverse directions. We also found that the shedding frequencies of vortex rings are much lower than the one corresponding to the appearance of leading-edge and lee-side vortices in the turbulent jets.

关键词： turbulent jet in crossflow coherent structure large-eddy simulation