检索结果-南通市图书馆

Effect of leukemia inhibitory factor on embryonic stem cell differentiation:implications for supporting neuronal differentiation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2006年第1期27卷 80-90页

作者： Zhao HE~(2,3. Jing-jing LI~(2,3. Chang-hong ZHEN~(3.4) Lin-ying FENG~3.Xiao-yan DING~(2,5)2 Laboratory of Molecular and Cell Biology and Laboratory of Stem Cell Biology,Institute of Biochemistry and Cell Biology:shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 20003.,china 3.shanghai Institute of Materia Medica.Chinese Academy of Sciences.shanghai 201203.china 4 Graduate School of the Chinese Academy of Sciences,shanghai 20003.,china. Laboratory of Molecular and Cell Biology and Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai China Graduate School of the Chinese Academy of Sciences Shanghai China

Aim:Leukemia inhibitory factor (LIF),a pleiotropic cytokine,has been used ex-tensively in the maintenance of mouse embryonic stem cell *** thiscurrent work,we examined the effect of the LIF signaling pathway in embryonicstem (ES) cell differentiation to a neural ***:In the presence of LIF(1000U/mL),the production of neuronal cells derived from embryoid bodies (EB)was tested under various culture *** of the LIF pathway wasexamined with specific *** effects of cell apoptosis and proliferationon neural differentiation were *** cell differentiation into three-germlayers was ***:Under various culture conditions,neuronal differ-entiation was increased in the presence of *** the LIF-activated STAT3.ignaling pathway with specific inhibitors abolished the neuronal differentiationof ES cells,whereas inhibition of the LIF-activated MEK signaling pathway im-paired the differentiation of ES cells toward a glial *** suppressed cellapoptosis and promoted cell proliferation during ES cell *** inhib-ited the differentiation of ES cells to both mesoderm and extraembryonic endo-derm fates,but enhanced the determination of neural ***:These results suggest that LIF plays a positive role during the differentiation ofES cells into neuronal cells.

关键词： leukemia inhibitory factor embryonic stem cells neural fate embryoid bodies

Progress in the detection of human genome structural variations

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china(Life Sciences) 2009年第6期52卷 560-567页

作者： WU XueMei1,3.& XIAO HuaSheng1,2 1 Center of Functional Genomics,Key Laboratory of System Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 20003.,china 2 National Engineering Center for Biochip at shanghai,shanghai 201203.china 3.Graduate School of the Chinese Academy of Sciences,shanghai 20003.,china Center of Functional Genomics Key Laboratory of System Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate School of the Chinese Academy of Sciences Shanghai China National Engineering Center for Biochip at shanghai Shanghai China

The emerging of high-throughput and high-resolution genomic technologies led to the detection of submicroscopic variants ranging from 1 kb to 3.Mb in the human *** variants include copy number variations(CNVs),inversions,insertions,deletions and other complex rearrangements of DNA *** paper briefly reviews the commonly used technologies to discover both genomic structural variants and their potential ***,we highlight the array-based,PCR-based and sequencing-based assays,including array-based comparative genomic hybridization(aCGH),representational oligonucleotide microarray analysis(ROMA),multiplex amplifiable probe hybridization(MAPH),multiplex ligation-dependent probe amplification(MLPA),paired-end mapping(PEM),and next-generation DNA sequencing ***,we discuss the limitations and challenges of current assays and give advices on how to make the database of genomic variations more reliable.

关键词： structural variation cytogenetic microarray PCR paired end mapping(PEM) next-generation sequencing

THE SCHWARZ CHAOTIC RELAXATION METHOD WITH INEXACT SOLVERS ON THE SUBDOMAINS

在线全文

学校读者我要写书评

暂无评论

Journal of Computational Mathematics 1999年第2期17卷 125-132页

作者： Jian-guo Huang(Department of Applied Mathematics, shanghai Jiao Tong University,shanghai 200240, china) 上海交通大学应用数学系上海

In this paper, a S-CR method with inexact solvers on the subdomains is presented, and then its convergence property is proved under very general conditions. This result is important because it guarantees the effective... 详细信息

关键词： S-CR method chaotic algorithm inexact solvers

Application of EGFP-EGF fusions to explore mechanism of endocytosis of epidermal growth factor

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第1期28卷 111-117页

作者： Hua JIANG~2 Jie ZHANG~(2,3. Bi-zhi SHI~(2,3. Yu-hong XU~3.Zong-hai LI~(2,3.4) Jian-ren GU~(2,3.4)2 State Key Laboratory of Oncogenes and Related Genes,shanghai Cancer Institute,Medical College of Fudan University 3.School of Pharmacy,shanghai Jiaotong University,shanghai 20003.,{3. State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute Medical College of Fudan University Shanghai China School of Pharmacy Shanghai Jiaotong University Shanghai China

Aim:To develop a simple method for monitoring protein localization of epidermalgrowth factor(EGF)in living ***:Enhanced green fluorescent protein(EGFP)was used as an autofluorescent tag to label EGF ***-PAGE andWestern blot analysis were used to detect the expression of the EGFP-tagged EGF(EGFP-EGF)*** cell-binding and internalization activity of EGFP-EGFwere analyzed by fluorescence-activated cell sorting(FACS)and confocal ***:EGFP-EGF protein was expressed in Escherichia coli and purified.A cell-binding assay demonstrated that the EGFP-EGF protein could bind effi-ciently to the cells expressing *** binding and internalization of EGFP-EGFcan be visualized even at a very low concentration under confocal *** FACS-based assay for internalization activity indicated the accumulation ofinternalized EGFP-EGF over ***,the results of the competitionassay indicated its EGFR binding *** such a method,it does notneed to label EGF with chemicals and avoid light in the experimental ***:The fusion protein EGFP-EGF has several characters including highsensitivity,stability and convenience for manipulation,and is a powerful tool forthe study of EGF endocytosis.

关键词： receptor epidermal growth factor endocytosis purification expression

A uniformly valid series solution to the unsteady stagnation-point flow towards an impulsively stretching surface

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china(Physics,Mechanics & Astronomy) 2010年第3期53卷 521-526页

作者： CHENG Jun & DAI ShiQiang shanghai Institute of Applied Mathematics and Mechanics, shanghai University, shanghai 200072, {3. shanghai Institute of Applied Mathematics and Mechanics Shanghai University Shanghai China

The paper studies the problem of the unsteady two-dimensional stagnation-point flow of an incompressible viscous fluid over a flat deformable sheet. The flow is started impulsively from rest and the sheet is suddenly stretched in its own plane with a velocity proportional to the distance from the stagnation point. An analytical series solution is obtained by means of the homotopy analysis method (HAM). Also, the homotopy-Pade′ technique is employed. An explicit formula for the local friction coefficient is provided. The present formula, different from the perturbation solution, is accurate and uniformly valid for all dimensionless time in the whole spatial region and for all possible values of physical parameter λ, defined as the ratio of the potential flow velocity to the sheet sudden stretching velocity. Numerical tests are done to verify the present formula for its validity and accuracy.

关键词： unsteady boundary-layer flows stagnation point stretching sheet homotopy analysis method

An Iterative Formula for the Reflection Coefficient of Multi-layer Thin Film and Its Application in the Design of Optical All Pass Filter

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Lasers 2002年第2期11卷 66-70页

作者： MENG Yichao TAN Weihan HUANG Zhaoming( Institute of Communication and Information, shanghai University, shanghai 200072, china Department of Physics, shanghai University, shanghai 20043., china) Institute of Communication and Information Shanghai University 上海 200072 Department of Physics Shanghai University 上海 200436

In this paper, an iterative formula for the reflection coefficient of the multi-layer thin film is deduced and the design of multi-layer thin film gires-tournois interferometer optical all pass filter(GTI-OAPF) is discussed. The group delay τm ranges from 0. 06 to 460 ps and the bandwidth △ω ranges from 0.068 to 0.0000079 (1015 rad/s). By changing the incident angle θ0, the multi-channel dispersion compensation may be achieved.

关键词： iterative formula for the reflection coefficient optical all pass filter GTI-OAPF

Mass spectrometry analysis of melanoma related O-glycans in sera

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china Chemistry 2010年第4期53卷 807-811页

作者： BAI Chen1,4, WU Gang1 & ZHAO Liang2,3.1Department of Life Science, Fudan University, shanghai 20043., china 2Department of Chemistry, Fudan University, shanghai 20043., china 3.nstitutes of Biomedical Sciences, Fudan University, shanghai 20043., china 4Department of Food Science, shanghai Business School, shanghai 20023., china Department of Life Science Fudan University Shanghai China Department of Food Science Shanghai Business School Shanghai China Department of Chemistry Fudan University Shanghai China Institutes of Biomedical Sciences Fudan University Shanghai China

Glycomics comparison was carried out by screening melanoma serum biomarkers between C57 mice with and without B16 implanted. O-glycans were released from 10 μL sera by β-elimination, purified by Graphitized Carbon Cartridge Solid Phase Extraction (GCC-SPE) and analyzed by MALDI-QIT-TOF-MS. MS raw data were acquired and exported by Launchpad software. MATLAB was then applied for further data analysis. 10 Glycans were considered to have stable changes after B16 implantation and 5 of them were under structural analysis via MS/MS.

关键词： mass spectrometry glycomics melanoma biomarkers

Protein Secondary Structure Prediction Using Dynamic Programming

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第3期37卷 167-172页

作者： Jing ZHAO Pei-Ming SONG Qing FANG Jian-Hua LUO School of Life Science & Technology,shanghai Jiaotong University,shanghai 200240,china shanghai Center for Bioinformation and Technology,shanghai 20023.,china Logistical Engineering University,Chongqing 400016,china School of Life Science & Technology Shanghai Jiaotong UniversityShanghai 200240 China shanghai Center for Bioinformation and Technologyshanghai 20023. China Logistical Engineering UniversityChongqing 400016 China

In the present paper,we describe how a directed graph was constructed and then searched forthe optimum path using a dynamic programming approach,based on the secondary structure propensity ofthe protein short sequence derived from a training data *** protein secondary structure was thus pre-dicted in this *** average three-state accuracy of the algorithm used was 76.70%.

关键词： directed graph dynamic programming approach protein secondary structure

A note on the 2-connectivity in one-dimensional ad hoc networks

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china(Information Sciences) 2011年第1期54卷 123-128页

作者： SHANG YiLun Department of Mathematics,shanghai Jiao Tong University, shanghai 200240,china Department of Mathematics Shanghai Jiao Tong University Shanghai China

2-connectivity is the basic graph theoretical metric for fault tolerance to node failures,and it is an extremely desirable property in network *** a one-dimensional finite ad hoc network formed by n nodes uniformly and independently distributed in a closed interval[0,z](z∈R+),we give a closed form expression for the probability that a connected one-dimensional finite ad hoc network becomes disconnected after removing a *** also sketch a procedure of finding the exact formula for 2-connectivity in a one-dimensional ad hoc network.

关键词： ad hoc network one-dimensional network 2-connectivity