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Study on low-temperature lipase of psychrophilic bacterium 2.5-10-1 isolated from deep sea of Southern ocean

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Acta oceanologica Sinica 2003年第4期22卷 643-649页

作者： Lin Xuezheng1,Yang Xiuxia2.Bian Ji1,Huang Xiaohang1 1. First Institute of oceanography,State oceanic Administration,{2. 2.6061,{3. 2. Fishery College, ocean university of china,{2. 2.6003.{3. State ocean Adm Inst Oceanog 1 Qingdao 266061 Peoples R China ocean Univ china Fishery Coll Qingdao 266003 Peoples R China

A strain of psychrophilic bacterium, 2.5-10-1, which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern ocean. The highest lipase secretion of this strain is observed at 5 degreesC and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 3. degreesC and 7.5 degreesC respectively. At 0degreesC, the lipase still has 3.% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 degreesC for 15 min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2. and Zn2..

关键词： Southern ocean deep sea psychrophilic bacteria low-temperature lipase

NUMERICAL SIMULATION OF SEA SURFACE DIRECTIONAL WAVE SPECTRA UNDER TYPHOON WIND FORCING

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Journal of Hydrodynamics 2008年第6期20卷 776-783页

作者： ZHOU Liang-ming WANG Ai-fang GUO Pei-fang Physical oceanography Laboratory Ocean University of China Qingdao 266100 China Engineering Reconnaissance and Design Institute Ocean University of China Qingdao 266071 China

Numercial simulation of sea surface directional wave spectra under typhoon wind forcing in the South china Sea （SCS） was carreid out using the WAVEWATCH-Ⅲ wave model. The simulation was run for 2.0 h until the Typhoon Damrey （2.05） approached Vietnam. The simulated data were compared with buoy observations, which were obtained in the northwest sea area of Hainan Island. The results show that the significant wave height, wave direction, wave length and frequency spetra agree well with buoy observations. The spatial characteristics of the signifciant wave height, mean wave period, mean wave length, wave age and directional spectra depend on the relative position from the typhoon center. Also, the misalignment between local wind and wave directions were investigated.

关键词： typhoon directional spectra significant wave height South china Sea (SCS)

Meso-scale circulation at the intermediate-depth east of Mindanao observed by Argo profiling floats

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Science china Earth Sciences 2010年第3期53卷 432-440页

作者： ZHOU Hui1, YUAN DongLiang1, GUO PeiFang2. SHI MaoChong2.& ZHANG QiLong 1 Key Laboratory of ocean Circulation and Wave Studies, Chinese Academy of Sciences, qingdao 2.6071,{2. 2.Physical oceanography Laboratory, ocean university of china, qingdao 2.6100,{2. 1. Key Laboratory of ocean Circulation and Wave Studies Chinese Academy of Sciences Qingdao 266071 China2. Physical Oceanography Laboratory Ocean University of China Qingdao 266100 China

The meso-scale circulation at the intermediate depth east of Mindanao is studied using Argo profiling floats observations. The trajectories and the parking-depth velocities of Argo floats show that the intermediate-depth circulation east of Mindanao contains significant meso-scale features that are highly variable both in space and in time. Both cyclonic and anticyclonic eddies at the intermediate depth (1000–2.00 m) are indicated by the trajectories east of Mindanao. The mean tangential velocities of these eddies are about 10 cm/s at 2.00 m and over 2. cm/s at 1000 m, which indicates that the geostrophic calculation may contain large errors due to the vigorous eddy activity at the reference levels. The analyses also suggest that these eddies might play an important role in mass and vorticity balances of the intermediate-depth circulation east of Mindanao.

关键词： Mindanao Current intermediate-depth circulation meso-scale eddy Argo profiling float

Codes From Strongly Regular (α,β)-reguli

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Journal of Shanghai Jiaotong university(Science) 2008年第4期13卷 491-494页

作者：李秀丽 Department of mathematics Shanghai Jiaotong University

Strongly regular (α,β)-reguli are a class of incidence structures with given conditions which were introduced by Hamilton and Mathon. We introduce two classes of codes constructed from strongly regular (α,β)-regul... 详细信息

关键词： two-weight codes strongly regular (α,β)-reguli

On Browkin's conjecture about the elements of order five in K_2.Q)

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Science china mathematics 2007年第1期50卷 116-120页

作者： Ke-jian XU Department of mathematics, qingdao {2.,{3., china 1. Department of mathematics Qingdao University Qingdao 266071 China

Based on the work of Lenstra, a succinct proof of Browkin’s conjecture about the elements of order five in K2.Q) is given.

关键词： K2 group cyclotomic polynomial lattice binary quartic form.

Compressing Dynamic Range of Wind Lidar Signals by Gate-control

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Chinese Journal of Lasers 2001年第6期10卷 40-46页

作者： WU Songhua LIU Zhishen LIU Jintao HE Yan SONG Xiaoquan (ocean Remote Sensing Laboratory of Ministry of Education of china, ocean {3. of qingdao, qingdao 2.6003. china) 青岛海洋大学海洋遥感所教育部海洋遥感重点实验室山东青岛 266003

Laser backscattering signals in the atmosphere have a dynamic range of 10 5 or 10 6, with the intensity varying from near-field to far-field, such as one to ten kilometers. The attenuation varies with the distance as exponential and inverse square law. Therefore, it is necessary to improve the dynamic range with a gate-control to compress laser backscattering signals. This paper presents a description of gate-control applications in a wind lidar which can compress the dynamic range of laser backscattering signals from the near field atmosphere. In addition, a photomultiplier with gate-controlling can improve the detection distance and signal-to-noise. The study on Gate-controlled photomultiplier EMI 92.4 has been carried out.

关键词： photomultiplier (PMT) dynode gate-control (gating)

Lattice dynamical simulation of methane hydrate

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Journal of Natural Gas Chemistry 2010年第1期19卷 43-46页

作者： Lin Wang, Shunle Dong Physics Department, College of Information Science and Engineering, ocean university of china, qingdao 2.6100, Shandong,{2. [a] Physics Department College of Information Science and Engineering Ocean University of China Qingdao 266100 Shandong China

INS （Inelastic Neutron Scattering） spectrum of methane hydrate was measured on MARI （a direct-geometry chopper spectrometer） at Ruther-ford Appleton Laboratory, UK. Compared with ice Ih, it is found that the whole spectrum of methane hydrate moves toward high-energy by about 1.5 meV. Using lattice dynamical （LD） technique, computer simulations of methane hydrate were carried out. In the simulations, four potential models （BE TIP3., TIP4P, MCY） were employed to calculate the phonon density of states （PDOS）. Comparing the calculated PDOS spectrum with the experimental spectrum, it is found that BF, TIP4P, and TIP3. potential lattices give out well-separated translational and librational bands while MCY potential lattice is unstable to do so and this model is not suitable to describe hydrate system.

关键词： methane hydrate inelastic neutron scattering lattice dynamical simulation

Observing seasonal variations of sea surface wind speed and significant wave height using TOPEX altimetry

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Chinese Science Bulletin 2000年第14期45卷 1323-1328,1345-1346页

作者： CHEN Ge & LIN Hui1. Laboratory of Remote Sensing Information Science, Institute of Remote Sensing Applications, Chinese Academy of Sciences, Beijing 100101, china 2. ocean Remote Sensing Institute, ocean {3. of qingdao, qingdao 2.6003. china 3. CAS/CUHK Joint Laboratory for Geo-Information Science and Department of Geography, the Chinese {3. of Hong Kong, Shatin, New Territories, Hong Kong, chinaCorrespondence should be addressed to Chen Ge Laboratory of Remote Sensing Information Science Institute of Remote Sensing Applications Chinese Academy of Sciences Beijing China ocean Remote Sensing Institute Ocean University of Qingdao Qingdao China CAS/CUHK Joint Laboratory for Geo-Information Science and Department of Geography The Chinese University of Hong Kong Shatin New Territories Hong Kong China

One year of ocean topography experiment (TOPEX) altimeter data are used to study the seasonal variations of global sea surface wind speed and significant wave height. The major wind and wave zones of the world oceans are precisely identified, their seasonal variability and characteristics are quantitatively analyzed, and the diversity of global wind speed seasonality and the variability of significant wave height in response to sea surface wind speed are also revealed.

关键词： satellite altimeter sea surface wind speed significant wave height seasonal variation.

Orthogonal design for optimization of pigment extraction from surface sediments of the Changjiang River Estuary

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Acta oceanologica Sinica 2011年第4期30卷 33-42页

作者： ZHAO Jun YAO Peng YU Zhigang Thomas S Bianchi Key Laboratory of Marine Environment and Ecology Ministry of Education Ocean University of China Qingdao 266100 China Department of oceanography Texas A ＆ M University College Station TX 77843 USA Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education Ocean University of China Qingdao 266100 China Institute of Marine Organic Geochemistry College of Chemistry and Chemical Engineering Ocean University of China Qingdao 266100 China

Using a suitable solvent for extracting pigments from sediment for high performance liquid chromatography（HPLC） analysis is critical for obtaining qualitative and quantitative estimates of phytoplanktonic and benthic algal biomass,as well as community composition. Five methodological factors（sample dehydration,extraction solvent,extraction duration,number of extractions,and ratio of solvent volume：sample weight） were studied using an L 9（3.4 ） orthogonal design in a sedimentary pigment extraction experiment on samples collected from the Changjiang large-river delta-front estuary（LDE）,using HPLC analysis. The results show that the optimal extraction method for sedimentary pigments should include freeze-drying samples prior to extraction. The effects of different factors on sedimentary pigment extraction were separated by the L 9（3.4 ） orthogonal design experiments and showed that the extraction solvent was the most important,with extraction duration the second most important,and numbers of extraction and ratio of solvent volume：sample weight was the least important. The mixed solvent treatment comprised of acetone,methanol and water（80：15：5,by volume） was best for polar pigment extraction,with 100% acetone better for apolar pigments. For most pigments employed in this study（i.e.,peridinin,fucoxanthin,alloxanthin,diatoxanthin,zeaxanthin,pheophytin-a and β-carotene） ,3.h was found to be enough time for extraction from these deltaic sediments. However,for chlorophyll-a,the most important pigment used for estimating algal biomass,12.h was needed. A small amount of solvent（3.ml） with duplicate extractions obtained the greatest amount and diversity of pigments. Unfortunately,no extraction method was found to be suitable for all pigments in sediments. The choice of extraction procedure should be made in accordance with the objective of each study,taking into consideration the properties of sediments and pigments in question.

关键词： sedimentary pigments extraction orthogonal design HPLC Changjiang River Estuary