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Tyroserleutide tripeptide affects calcium homeostasis of human hepatocarcinoma BEL-74.2 cells

Science China(Life Sciences) 2005年第5期48卷 523-530页

作者： FU Zheng1, LU Rong2, LI Guoli1, ZHAO Lan1, GAO Weizhen3. CHE Xuchun1, JIAN Xu4. ZHOU Chunlei1 & YAO Zhi1 1. department of Immunology, tianjin medical university, tianjin 300070, China 2. Shenzhen Kangzhe Pharmaceutical Co. Ltd., Shenzhen, 518029, China {3., tianjin medical university, tianjin 300070, China 4. Central Laboratory of tianjin medical university Hospital, tianjin 300070, China department of Immunology Tianjin Medical University Tianjin China Shenzhen Kangzhe Pharmaceutical Co. Ltd. Shenzhen China department of pharmacy Tianjin Medical University Tianjin China Central Laboratory of tianjin medical university Hospital Tianjin China

This study aimed to observe the effects of tyroserleutide (tyrosyl-seryl-leucine, YSL) on the growth of human hepatocarcinoma BEL-74.2 that was transplanted into nude mice, and explore its anti-tumor mechanism preliminarily. YSL, at doses of 80 μg·kg?1·d?1, 160 μg·kg?1·d?1 and 3.0 μg·kg?1·d?1 significantly inhibited the growth of the human hepatocarcinoma BEL-74.2 tumor in nude mice, producing inhibition of 21.66%, 4..3.%, and 3..78%, respectively. Ultra structure of BEL-74.2 tumor in nude mice showed that YSL could induce tumor cells apoptosis and necrosis, cell organelle mitochondria and endoplasmic reticulum damage, and calcium over-load. By confocal laser scanning microscopy and flow cytometry, we found that 10 μg/mL YSL rapidly induced an increase of the concentration of cytoplasmic free calcium in BEL-74.2 cells in vitro, and maintained high concentrations of cytoplasmic free calcium for 1 h. Then the calcium concentration began to decrease after 2 h, and was lower than that of the control group at 4.h and 24.h (p4.2 cells in vitro, but had no effect on the calcium homeostasis or mitochondrial transmembrane potential of Chang liver hepatocytes. So affecting calcium homeostasis, then inducing apoptosis and necrosis may be a mechanism by which YSL inhibits the tumor growth in animal model.

关键词： tyroserleutide, YSL, human hepatocarcinoma BEL-74.2, cytoplasmic free calcium, mitochondrial transmembrane potential.

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The Citron homology domain of MAP4.s improves outcomes of traumatic brain injury

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Neural Regeneration Research 2025年第11期20卷 3233-3244页

作者： Xiaoling Zhong Wenjiao Tai Meng-Lu Liu Shuaipeng Ma tianjin Shen Yuhua Zou Chun-Li Zhang department of Molecular Biology University of Texas Southwestern Medical CenterDallasTXUSA Hamon Center for Regenerative Science and Medicine University of Texas Southwestern Medical CenterDallasTXUSA Peter O’Donnell Jr.Brain Institute University of Texas Southwestern Medical CenterDallasTXUSA

The mitogen-activated protein kinase kinase kinase kinases(MAP4.s)signaling pathway plays a pivotal role in axonal regrowth and neuronal degeneration following *** targeting this pathway is beneficial to brain injury remains *** this study,we showed that adeno-associated virus-delivery of the Citron homology domain of MAP4.s effectively reduces traumatic brain injury-induced reactive gliosis,tauopathy,lesion size,and behavioral *** inhibition of MAP4.s replicated the ameliorative effects observed with expression of the Citron homology ***,the Citron homology domain acted as a dominant-negative mutant,impeding MAP4.-mediated phosphorylation of the dishevelled proteins and thereby controlling the Wnt/β-catenin *** findings implicate a therapeutic potential of targeting MAP4.s to alleviate the detrimental effects of traumatic brain injury.

关键词： adeno-associated virus Citron homology Citron homology domain gene therapy mitogen-activated protein kinase kinase kinase kinases traumatic brain injury

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Effect of CO_2 laser sclerectomy with iridectomy on ocular hypertension in rabbits

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International Journal of Ophthalmology(English edition) 2011年第1期4卷 30-32页

作者： Zhi-Xiao Xue 1,3.Ying-Xin Li 2,3.Yan-Shan Xu 4.Jiu-Min Yang 1 1 Laser Medicine Laboratory of tianjin medical university,tianjin {4.,China 2 Laser Medicine Laboratory,Bio{3. Engineering Institute of Chinese Academy of {3. Sciences & Peking Union {3. College,tianjin 3.0192,China 3.Laser Medicine Technology Engineering Center of tianjin,tianjin 3.0192,China 4.Eye Center of tianjin medical university,tianjin 3.03.4.China Laser Medicine Laboratory of tianjin medical university Tianjin 300070 China.

AIM:To evaluate the efficiency and safety of an optimized CO2 Laser glaucoma surgery system for laser sclerectomy with ***:Rabbit trials were performed to evaluate the efficiency and ***:IOP was significantly decreased in laser group compared with trabeculectomy group(P<0.05) from 7th postoperative day to 60th *** with trabeculectomy group,histopathology studies confirmed fewer complications and better effects were found in laser ***:CO2 laser sclerectomy with iridectomy is effective and safe in terms of IOP lowering.

关键词： CO2 laser glaucoma sclerectomy with irid-ectomy intraocular pressure histopathological examination

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Two-photon spectroscopic behaviors and photodynamic effect on the BEL-74.2 cancer cells of the new chlorophyll photosensitizer

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Science China Chemistry 2008年第6期51卷 529-538页

作者： ZHAO PeiDe1,2,CHEN Ping1,TANG GuoQing1,LIU JinWei2,LIN Lie1,GUO Peng1,YU Qing2, YAO JianZhong3.MA DongMing4.ZHANG GuiLan1 & CHEN WenJu1 1 Key Laboratory of Optoelectronic Information Science and Technology,Ministry of Education,Institute of Modern Optics,Nankai university,tianjin 3.0071,China 2 {4. of Physics,Hebei university of Technology,tianjin 3.013.,China 3.College of pharmacy,Second Military medical university,Shanghai 2004.0},China 4.tianjin College of the Traditional Chinese Medicine,tianjin 3.0193.China Key Laboratory of Optoelectronic Information Science and Technology Ministry of Education Institute of Modern Optics Nankai University Tianjin China department of Physics Hebei University of Technology Tianjin China College of pharmacy Second Military Medical University Shanghai China tianjin College of the Traditional Chinese Medicine Tianjin China

The spectroscopic properties of a new chlorophyll derivate photosensitizer (CDP) are studied under the excitation wavelengths at 800 and 4.0 nm using femtosecond pulses from a Ti: sapphire laser. The damaging effect of CDP on the BEL-74.2 cancer cells is also investigated upon two-photon illumination at 800 nm. The normalized fluorescence spectra of CDP in tetrahydrofuran (THF) show that two-photon and one-photon spectra have the same distributions and the same emission bands (675 nm). The lifetimes of two- and one-photon induced fluorescence of this molecule are of the order of 5.0 ns. By comparing the data it is shown that there is some difference between the two lifetimes, but the difference is less than one nanosecond. The two-photon absorption cross section of the molecule is also measured at 800 nm and estimated as about σ'_2 ≈ 3..5x10^(-50)cm^4.s·photon^(-1). The results of two-photon photodynamic therapy (TPPDT) tests show that CDP can kill all of the tested cancer cells according to the usual Eosine assessment. Our results indicate that the two-photon-induced photophysical, photochemical and photosensitizing processes of CDP may be basically similar to those of one-photon excitation. These behaviors of the sample suggest that one may find other possible methods to estimate some photosensitizers' effects in details such as their distribution in cells and the reactive targets of the sub-cellular parts of some tumor cells via two-photon excitation techniques.

关键词： photosensitizer chlorophyll derivate two-photon fluorescence two-photon absorption cross-section TPPDT BEL-74.2 cell

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Inhibition of Proliferation and Induction of Apoptosis in Human Renal Carcinoma Cells by Anti-telomerase Small Interfering RNAs

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 500-506页

作者： Jun-Nian ZHENG~(1,3.*)Ya-Feng SUN~(2#)Dong-Sheng PEI~2 Jun-Jie LIU~1 Jia-Cun CHEN~1 Wang LI~1 Xiao-Qing SUN~1 Qi-Duo SHI~3.Rui-Fa HAN~3.Teng-Xiang MA~3. 1 Laboratory of Urology,Affiliated Hospital of Xuzhou {4. College,Xuzhou 221002,China 2 Research Center for Biochemistry and Molecular Biology,Xuzhou {4. College,Xuzhou 221002,China 3.Institute of Urology,tianjin {4. {4.,tianjin 300070,China Laboratory of Urology Affiliated Hospital of Xuzhou Medical CollegeXuzhou 221002 China Institute of Urology Tianjin Medical UniversityTianjin 300070 China Research Center for Biochemistry and Molecular Biology Xuzhou Medical CollegeXuzhou 221002 China

Telomerase is an attractive molecular target for cancer therapy because it is present in mostmalignant cells but is undetectable in most normal somatic *** telomerase consists of two subunits,an RNA component(hTR)and a human telomerase reverse transcriptase component(hTERT).Smallinterfering RNA(siRNA),one kind of RNA interferences,has been demonstrated to be an effective methodto inhibit target gene expression in human *** investigated the effects of siRNA targeting at both hTRand hTERT mRNA on the inhibition of telomerase activity in human renal carcinoma cells(HRCCs).Theproliferation and apoptosis of HRCCs were *** treatment of HRCCs using hTR and hTERTsiRNAs resulted in significant decrease of hTR mRNA,hTERT mRNA and hTERT *** siRNA canalso inhibit the telomerase activity and the proliferation of ***,they can induce apoptotic celldeath in a dose-dependent *** these findings,we propose that the inhibition of telomerase activityusing siRNA targeting hTR and hTERT might be a rational approach in renal cancer therapy.

关键词： hTERT hTR telomerase siRNA renal cell carcinoma proliferation apoptosis

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人血浆脂蛋白B∶E的测定

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生物化学与生物物理进展 1997年第3期24卷 260-264页

作者：解用虹郭刚天津医科大学生物化学教研室!天津300070 天津医科大学代谢病防治中心!天津300070

用纯化的羊抗人载脂蛋白B抗体包被 96孔微量滴定板 ,与样品保温后 ,加入辣根过氧化物酶标记的羊抗人载脂蛋白E纯化抗体 ,最后用邻苯二胺底物显色 .与同时进行的标准物比较 ,可测定样品中含有载脂蛋白B脂蛋白中载脂蛋白E (简称LpB∶E )... 详细信息

用纯化的羊抗人载脂蛋白B抗体包被 96孔微量滴定板 ,与样品保温后 ,加入辣根过氧化物酶标记的羊抗人载脂蛋白E纯化抗体 ,最后用邻苯二胺底物显色 .与同时进行的标准物比较 ,可测定样品中含有载脂蛋白B脂蛋白中载脂蛋白E (简称LpB∶E )的含量 .用建立的方法对 1 2 0例血样进行了LpB∶E测定 ,并对所得结果进行了简要讨论 .

关键词：脂蛋白载脂蛋白酶联免疫吸附测定

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FKBP23.in ER binds to BiP specifically

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Science China Chemistry 2004年第1期47卷 17-23页

作者： ZHANG Wanqi, LI Hui, ZHANG Xiaobin, SHEN Ye, WANG Yinong, Ml Huaifeng & HE BinglinState Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Chemical School of Nankai university, tianjin 3.0071, China Hygienic School of tianjin medical university, tianjin 3.0071, China {4. School of Nankai university, tianjin 3.0071, China State Key Laboratory of Functional Polymer Materials for Adsorption and Separation Chemical School of Nankai University Tianjin China Hygienic School of tianjin medical university Tianjin China medical School of Nankai university Tianjin China

FKBP23.was found in mouse endoplasmic reticulum (ER) in 1998. It consists of an N-terminal peptidyl-prolyl cis-trans isomerase (PPlase) domain and a C-terminal domain with Ca2+ binding sites. The fusion protein of mouse FKBP23.and glutathione S-transferase (GST), GST-FKBP23. and the fusion protein of BiP, a member of heat shock protein 70 (Hsp 70) in ER, and GST, GST-BiP, were subcloned in E. coli, expressed and purified. The fusion proteins were restrictively digested by Factor Xa (FaXa) to obtain the free cloned proteins FKBP23.and BiP. With the assay of adsorption of free FKBP23.or BiP with GST-BiP or GST-FKBP23.attached to the Glutathione-Sepharose 4., the adsorbed FKBP23.or BiP could be detected by Immunoblot. It means that FKBP23.binds to BiP. Furthermore, BiP in leukocyte ER-extract can be adsorbed with GST-FKBP23.attached to the glutathione-Sepharose 4.. It shows that FKBP23.binds to natural BiP in ER, too. These experiments show that a PPIase binds to a molecular chaperone of the Hsp70 family.

关键词： peptidyl-prolyl cis/trans isomerase, FKBP23. BiP, endoplasmic reticulum, specific binding.

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Etiology and management of hemmorrhage in spontaneous liver rupture:a report of 70 cases

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World Journal of Gastroenterology 2002年第6期8卷 1063-1066页

作者： Zhe-Yu Chen Qing-Hui Qi Zuo-Liang Dong,department of surgery,General Hospital of tianjin medical university,tianjin 3.0052,China department of surgery General Hospital of Tianjin Medical University China.

AIM: To analyze the causes and management of hemorrhage in spontaneous liver ***: Seventy cases of spontaneous liver rupture were retrospectively analyzed for causes of hemorrhage and therapeutic effects of surgical ***: It was demonstrated that the causes of spontaneous liver rupture were primary liver cancer in 60 cases (85.7 %), cirrhosis in 3.cases (4.3.%), liver angioma in 2 cases (2.9 %), liver adenoma in 4.cases (5.7 %),and secondary liver cancer in 1 case (1.4.%). Hemostasis was achieved with surgical approaches in 68 cases (97.1 %) and non-surgical approaches in 2 cases (2.9 %). Surgical interventions induded suture, ligation of hepatic artery, hepatic artery chemoembolization and partial hepatic ***: The results suggest that surgical intervention is still the main therapeutic method and the best procedure that should be selected according to causes of disease and patient's condition and history.

关键词：自发性肝破裂出血病因手术管理

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Repair effect of diabetic ulcers with recombinant human epidermal growth factor loaded by sustained-release microspheres

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Science China(Life Sciences) 2008年第11期51卷 1039-1044页

作者： DONG XiaoQing1, XU Jun2, WANG WeiCai1, Luo Hao1, LIANG XiaoFei1, Zhang Lei1, Wang HanJie1, Wang PengHua2 & CHANG Jin1 1 Institute of Nanobiotechnology, School of Materials Science and Engineering, tianjin university, tianjin 3.0072, China 2 {3. of Diabetic Foot,{4. Metabolic Disease Hospital, tianjin 300070, China Institute of Nanobiotechnology School of Materials Science and Engineering Tianjin University Tianjin China department of Diabetic Foot Tianjin Medical University Metabolic Disease Hospital Tianjin China

In this study the w/o/w extraction–evaporation technique was adopted to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres loading recombinant human epidermal growth factor (rhEGF). The micro-spheres were characterized for morphology by transmission electron microscopy (TEM) and particle size distribution. The release performances, the proliferation effects and therapeutic effects of rhEGF-loaded PLGA microspheres were all studied. The results showed that these spherical micro-spheres had a narrow size distribution and a high drug encapsulation efficiency (85.6%). RhEGF-loaded microspheres enhanced the growth rate of fibroblasts and wound healing more efficiently than pure rhEGF. The number of the proliferating cell nuclear antigen (PCNA) in the epidermis layer with the mi-crosphere treatment was significantly larger than those of the control groups. Overall locally sustained delivery of rhEGF from biodegradable PLGA microspheres may serve as a novel therapeutic strategy for diabetic ulcer repair.

关键词： rhEGF microspheres the diabetic ulcer recombinant human epidermal growth factor PLGA

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Canals in the Roof of the Pharynx :Anatomic Study and their Clinical Meaning

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生物医学工程学杂志 2005年第S1期22卷 39-40页

作者： Ying JIN Yun-Sheng LI~Δ De-Run TIAN Chang-Yue WANG(department of Anatomy, tianjin medical university, tianjin 300070,China)

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