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Effect of active immunization with peptides corresponding to the second extracellular loop of alpha1-adrenoceptor on vascular function in rats

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Effect of active immunization with peptides corresponding to...

中国生理学会第23.全国会员代表大会暨生理学学术大会

作者： Li YAN~1,Zhong-Hua DAI~1,Guang-Zhao YANG~2,Su-Li ZHANG~2,Hui-Rong LIU~(2,3.,Ji-Min CAO~1 1 Department of Physiology and Pathophysiology,Institute of Basic Medical sciences,Chinese Academy of Medical sciences,School of Basic Medicine,Peking union Medical {3.,beijing 100005,china 2 Department of Physiology,Shanxi Medical university, Taiyuan,Shanxi,china 3.Department of Pathophysiology,School of Basic Medical sciences,Capital Medical university,beijing, 100069,china

Backgroud:Autoantibody against the second extracellular loop ofα-adrenoceptor(α-AA) which had been found in hypertensive patients possess agonist-like activity as phenylephrine evidenced by previous studies, however,long-term stimulation ofα-AA did not modify the mean arterial pressure in spite of the occurrence of cardiac and vascular remodeling,and the mechanism was not fully ***:In this study,the effect of acuteα-AA administration on mean arterial blood pressure was observed;the models with antibodies against theα-adrenoceptor were established by immunizing Wistar rats with the synthesized peptide corresponding to the second extracellular loop ofα-adrenoceptor(α-AR-EC).The age-matched male Wistar rats received a mixture of saline and immunological adjuvant as sham immunized *** the rats were raised for 8 *** blood pressure and heart rate were measured every *** reactivity experiments were performed in segments of thoracic ***-1(ET-1) concentration and iNOS expression were determined by ELISA and immunochemistry ***:The mean arterial pressure significantly increased after the administration ofα- AA in *** the systolic blood pressure of immunized rats having no difference with sham group,the isolated thoracic arteries showed hyperresponsiveness to phenylephrine and decreased endothelium-dependent relaxation at 6 and 8 months after initial ***,the plasma endothelin-1(ET-1) concentration and iNOS expression in vascular endothelium from the thoracic aorta increased,indicating that the long-term stimulation might contribute to endothelium injury,which plays an important role in the vasomotor dysfunction and enhanced pressor ***:The results demonstrated thatα-AA could contribute to vasomotor dysfunction in rats by vascular endothelial damage and increase iNOS expression,and might play a particular role in the pathological changes of hypertension.

关键词： receptor,adrenergic,alpha1 autoantibody vascular dysfunction hypertension

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比较诗学的名与实

深圳大学学报（人文社会科学版） 2005年第1期22卷 108-113页

作者：郁龙余刘朝华深圳大学文学院广东深圳518060 北京大学外国语学院北京100871

比较诗学是当代中国比较文学研究的最大热点,但普遍存在对安田朴的比较诗学概念的“误解”。从学科建设的实际看,比较诗学之实在前,名在后,所谓1963.安田朴预言产生比较诗学,是一个现代学术童话。因此,要搞好比较诗学研究,必须破除西... 详细信息

比较诗学是当代中国比较文学研究的最大热点,但普遍存在对安田朴的比较诗学概念的“误解”。从学科建设的实际看,比较诗学之实在前,名在后,所谓1963.安田朴预言产生比较诗学,是一个现代学术童话。因此,要搞好比较诗学研究,必须破除西方中心主义;“左手必须知道右手在做什么”;必须从“知其二”迈向“察其三”,坚持“第三者原则”。

关键词：比较诗学名与实思考

Endothelial modulation of the alpha1 adrenoceptor autoantibody-induced arterial contraction in spontaneously hypertensive rats

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Endothelial modulation of the alpha1 adrenoceptor autoantibo...

中国生理学会第23.全国会员代表大会暨生理学学术大会

作者： Zhong-Hua DAI~1,Li YAN~1,Guang-Zhao YANG~2,Su-Li ZHANG~2,Hui-Rong LIU~(2,3.,Ji-Min CAO ~1 1 Department of Physiology and Pathophysiology,Institute of Basic Medical sciences,Chinese Academy of Medical sciences,School of Basic Medicine,Peking union Medical {3.,beijing 100005,china 2 Department of Physiology,Shanxi Medical university, Taiyuan,china 3.Department of Pathophysiology,School of Basic Medical sciences,Capital Medical university,beijing,100069, china

Background:Growing evidence indicates that primary or secondary activation of immune mechanisms have been implicated in the pathogenesis of many forms of *** previous studies have demonstrated that the autoantibodies against the second extracellular loop ofα-adrenoceptor(α-AA) existed in the sera from patients with primary hypertension and possessed agonist-like vasoconstrictive *** study was designed to observe that whetherα-AA exhibited the similar vasocontractive characteristics on isolated thoracic artery rings from normotensive and hypertensive *** so,we further explore whether the endothelium and nitric oxide play the altered negative regulation onα-AA-induced contraction in hypertension and the possible ***: IgGs were purified from sera ofα-AA positive hypertensive patients and 20α-AA negative normotensives respectively,and its purification,concentration and titer were detected by SDS-PAGE,BCA assay and ELISA, respectively;vascular reactivity experiments were performed in segments of thoracic aorta from normotensive Wistar Kyoto(WKY) rats and spontaneously hypertensive rats(SHR).The levels of protein nitration expressions in aorta were measured by ***:The contractile responses toα-AA(0.1-100 mmol/L) were greater in aortic segments from SHR than WKY(P3. mmol/L).Both the removal of endothelium and the non-selective NOS inhibitor, L-NAME(100μmol/L),further increased both phenylephrine andα-AA contraction in both the WKY and SHR segment(P0.05).In addition,nitrotyrosine formation was greater in aorta from SHR than that from WKY(P<0.05).Conclusion:The results indicated that reduced endothelium inhibition onα-AA-induced vasoconstriction and decreased bioavailability of NO,which co

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MPP+ decreased BDNF expression in PC12 cells

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MPP+ decreased BDNF expression in PC12 cells

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Jian-Dong SUN,Nai-Hong CHEN~* Department of Pharmacology,Institute of Materia Medica,Chinese Academy of Medical sciences and Peking union Medical college,{3. 100052,china

The aim of this study is to investigate the neurotoxic effect and mechanism of l-methyl-4-phenyl- pyridinium (MPP) on PC12 *** assay was used to investigate cell viability,Western blotting assay was performed to observe the protein level and phosphorylation,and dual-luciferase assay was used to study the *** experiment showed that MPP could decrease cell viability significantly in a dose-dependent manner and could decrease BDNF protein level,depress the phosphorylation of ERK,and attenuate the phosphorylation and transactivation of CREB,which is one of transcription factors of BDNF,but did not affect the activity of CaMK II in PC12 *** MPP might decrease BDNF protein level through MAPK/ERK signal pathway.

关键词： MPF BDNF ERK CREB

Overexpressedα-synuclein regulated the nuclear factor-κB signal pathway

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Overexpressedα-synuclein regulated the nuclear factor-κB s...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yu-He YUAN,Nai-Hong CHEN~* Department of Pharmacology,Institute of Materia Medica,Chinese Academy of Medical sciences and Peking union Medical college,{3. 100052,china

oc-Synuclein is a presynaptic protein which is implicated in some neurodegenerative disorders including Parkinson’s disease,dementia with Lewy bodies,multiple systems atrophy,and Hallervorden-Spatz disease,and its overexpression contributes to the loss of dopaminergic *** the role ofα-synuclein in these paradigms has been widely documented,its exact function is still *** the dysfunction of the transcription factor nuclear factor (NF-κB) also exists in many neurodegenerative *** this reason the purpose of this study was to investigate the molecular mechanism ofα-synuclein’s toxicity and its association with NF-κB by MTT assay,Western blot method,and luciferase *** showed that overexpressedα-synuclein and 1-methyl-4-phenylpyridinium(MPP suppressed the SH-SY5Y cell viability and attenuate NF-κB-mediated luciferase expression ***,the impairment function was enhanced with the increase ofα-synuclein protein *** also found that overexpressedα-synuclein localized both in the cytoplasms and nuclei,down-regulated the anti-apoptotic Bcl-2 expression and up-regulated the proapoptotic glycogen synthase kinase 3.(GSK3.) protein *** conclusion,all these findings mentioned above suggested thatα-synuclein shared some toxic functional homology with neurotoxin MPP,and the proapoptotic effects of alpha-synuclein might be mediated at least in part by the impairment of NF-κB signaling pathway which involves GSK3..

关键词： α-synuclein NF-κB MPP GSK3. Bcl-2

The genetic load for hereditary hearing impairment in Chinese population and its clinical implication

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Journal of Otology 2009年第2期4卷 98-105页

作者： WANG Qiu-ju 1,2, RAO Shao-qi 1, 3. GUO Yu-fen 4, LI Qing-zhong 5, ZHAO Hui 1, ZHAO Li-dong 1, YUAN Hu 1, ZONG Liang 1, LIU Qiong 1, ZHAO Ya-li 6, WANG Da-yong 1, HAN Ming-kun 1, JI Yu-bin 1, LI Jian-qiang 1, LAN Lan 1, YANG Wei-yan 1, SHEN Yan 2,6, HAN Dong-yi 1 1 Department of Otorhinolaryngology-Head and Neck Surgery, and Institute of Otolaryngology, Chinese People’s Liberation Army General Hospital, beijing, 100853.china 2 Chinese National Human Genome Center, beijing, 100176 china 3.Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-Sen university, Guangzhou, 510080, china 4 Department of Otorhinolaryngology, Head and Neck Surgery, Second Hospital of Lanzhou university, Lanzhou 73.03., china 5 Department of Otolaryngology, EYE & ENT hospital of Fudan university, Shanghai, 20003.,china 6 Institute of Basic Medical sciences, Chinese Academy of Medical sciences and Peking union Medical {13., beijing, 100005 china Department of Otorhinolaryngology–Head and Neck Surgery and Institute of Otolaryngology Chinese People’s Liberation Army General Hospital Beijing 100853 China Chinese National Human Genome Center Beijing 100176 China Department of Medical Statistics and Epidemiology School of Public Health Sun Yat–Sen University Guangzhou 510080 China Department of Otorhinolaryngology Head and Neck Surgery Second Hospital of Lanzhou UniversityLanzhou 730030 China Department of Otolaryngology EYE & ENT hospital of Fudan University Shanghai 200031China Institute of Basic Medical sciences Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100005 China

Objective To understand the genetic load in the Chinese population for improvement in diagnosis, prevention and rehabilitation of deafness. Methods DNA samples, immortalized cell lines as well as detailed clinical and audiometric data were collected through a national genetic resources collecting network. Two conventional genetic approaches were used in the studies. Linkage analysis in X chromosome and autosomes with microsatellite markers were performed in large families for gene mapping and positional cloning of novel genes. Candidate gene approach was used for screening the mtDNA 12SrRNA, GJB2 and SLC26A4 mutations in population -based samples. Results A total of 2,572 Chinese hearing loss families or sporadic cases were characterized in the reported studies, including seven X-linked, one Y-linked, 28 large and multiplex autosomal dominant hearing loss families, 607 simplex autosomal recessive hereditary hearing loss families, 100 mitochondrial inheritance families, 147 GJB2 induced hearing loss cases, 23. cases with enlarged vestibular aqueduct (EVA) syndrome, 169 sporadic cases with auditory neuropathy, and 1,283.sporadic sensorineural hearing loss cases. Through linkage analysis or sequence analysis, two X-linked families were found transmitting two novel mutations in the POU3.4 gene, while another X -linked family was mapped onto a novel locus, nominated as AUNX1 (auditory neuropathy, X-linked locus 1). The only Y-linked family was mapped onto the DFNY1 locus(Y-linked locus 1, DFNY1). Eight of the 28 autosomal dominant families were linked to various autosomal loci. In population genetics studies, 2,567 familial cases and sporadic patients were subjected to mutation screening for three common hearing loss genes: mtDNA 12S rRNA 1555G, GJB2 and SLC26A4. The auditory neuropathy cases in our samples were screened for OTOF gene mutations. Conclusions These data show that the Chinese population has a genetic load on hereditary hearing loss. Establishing personalize

关键词： Hereditary hea ring loss linkage analysis DFNY1 AUNX1 auditory neuropathy enlarged vestibular aqueduct senserineural hearing loss genetic epidemiology

mTOR enhancement of STIM1-mediated store-operated Ca2+ signaling constrains tumor development

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mTOR enhancement of STIM1-mediated store-operated Ca2+ signa...

第九届全国钙信号和细胞功能研讨会

作者： Haiyong Peng~1,Jie Liu~2,Jun Wang~2,Hongbing Zhang~1 1 Institute of Basic Medical sciences and School of Basic Medicine,Chinese Academy of Medical sciences and Peking union Medical {3.,beijing,china. 2 Department of Physiology,Capital Medical university,beijing,china

RTK-PI3.-AKT-TSC1/TSC2-mTOR pathway is frequently dysregulated in cancer. Loss-of-function mutations in either the TSCl or TSC2 tumor suppressor gene cause Tuberous Sclerosis Complex(TSC),an autosomal dominant disorder featured with benign tumors in multiple *** l and TSC2 form a protein complex as a key negative regulator of *** though hyperactive mTOR signal ing drives tumor *** critical effectors downstream of mTOR leading to tumor growth are

关键词： mTOR SOCE Tumor

Plant DNA barcoding in china

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Journal of Systematics and Evolution 2011年第03期49卷 165-168页

作者： 1De-Zhu LI 2Jian-Quan LIU 3.hi-Duan CHEN 1Hong WANG 4Xue-Jun GE 3.hi-Liang ZHOU 1Lian-Ming GAO 5Cheng-Xin FU 6Shi-Lin CHEN 1(Plant Germplasm and Genomics Centre,Germplasm Bank of Wild Species in Southwest china Key Laboratory of Biodiversity and Biogeography,Kunming Institute of Botany,Chinese Academy of sciences,Kunming 650204,china) 2(Key Laboratory of Arid and Grassland Ecology,{3. of Life sciences,Lanzhou university,Lanzhou 73.000,china) 3.State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of sciences,beijing 100093.china) 4(Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South china Botanical Garden,Chinese Academy of sciences,Guangzhou 510650,china) 5(Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education,{3. of Life sciences,Zhejiang university,Hangzhou 3.0058,china) 6(Institute of Medicinal Plant Development,Chinese Academy of Medical sciences,Peking union Medical {3.,beijing 100193.china) (Plant Germplasm and Genomics Centre Germplasm Bank of Wild Species in Southwest China Key Laboratory of Biodiversity and Biogeography Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (Key Laboratory of Arid and Grassland Ecology College of Life Sciences Lanzhou University Lanzhou 730000 China) (State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 China) (Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education College of Life Sciences Zhejiang University Hangzhou 310058 China) (Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100193 China) *Correspondence: De-Zhu LI *Correspondence: ?Author for correspondence. E-mail: dzl@*** Tel.: 86-871-5223.03 Fax: 86-871-5217791.

Identification is the keystone of biology(Bell, 1986).However,to biologists and students of biology, the total numbers of species that must be identified far outnumber the names commonly used in English,Chinese,or oth... 详细信息

关键词： DNA Plant DNA barcoding in china matK

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论数字影像及其真实性观念

上海大学学报（社会科学版） 2002年第1期9卷 31-37页

作者：潘秀通潘源上海大学影视艺术技术学院上海200072 北京科技大学外语系北京100083

数字影像对传统电影电视的巨大冲击 ,乃是在制作方式、生成方式与存储介质上的划时代的革命意义。尽管它竟可“以假乱真” ,但是依然不能改变人们对数字影像的真实性要求。影视制作者依然必须根据生活的真实、艺术的真实抑或感觉的真实... 详细信息

数字影像对传统电影电视的巨大冲击 ,乃是在制作方式、生成方式与存储介质上的划时代的革命意义。尽管它竟可“以假乱真” ,但是依然不能改变人们对数字影像的真实性要求。影视制作者依然必须根据生活的真实、艺术的真实抑或感觉的真实来生成数字化虚构影像的创造性真实。真实永远是一切艺术的生命。电影无论在中国乃至全球 ,都不会在短期内步入“后电影时代”而濒临消亡。

关键词：数字影像艺术功能美学观念真实性观念后电影时代