Overexpressedα-synuclein regulated the nuclear factor-κB signal pathway
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学]
关 键 词：α-synuclein NF-κB MPP GSK3β Bcl-2
摘 要：oc-Synuclein is a presynaptic protein which is implicated in some neurodegenerative disorders including Parkinson’s disease,dementia with Lewy bodies,multiple systems atrophy,and Hallervorden-Spatz disease,and its overexpression contributes to the loss of dopaminergic *** the role ofα-synuclein in these paradigms has been widely documented,its exact function is still *** the dysfunction of the transcription factor nuclear factor (NF-κB) also exists in many neurodegenerative *** this reason the purpose of this study was to investigate the molecular mechanism ofα-synuclein’s toxicity and its association with NF-κB by MTT assay,Western blot method,and luciferase *** showed that overexpressedα-synuclein and 1-methyl-4-phenylpyridinium(MPP suppressed the SH-SY5Y cell viability and attenuate NF-κB-mediated luciferase expression ***,the impairment function was enhanced with the increase ofα-synuclein protein *** also found that overexpressedα-synuclein localized both in the cytoplasms and nuclei,down-regulated the anti-apoptotic Bcl-2 expression and up-regulated the proapoptotic glycogen synthase kinase 3β(GSK3β) protein *** conclusion,all these findings mentioned above suggested thatα-synuclein shared some toxic functional homology with neurotoxin MPP,and the proapoptotic effects of alpha-synuclein might be mediated at least in part by the impairment of NF-κB signaling pathway which involves GSK3β.