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Absolute radiometric calibration of CBERS-02.IRMSS thermal band

Science china(Technological Sciences) 2005年第S2期48卷 72-90页

作者： ZHANG Yong1,2.3. GU Xingfa1,3.4, YU Tao1,3. ZHANG Yuxiang5, CHEN Liangfu1,3. LI Xiaoying1,2.3. LI Xiaowen1,3.& HE Liming6 1. State Key Laboratory of Remote Sensing Science, Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University, Beijing 100101, china 2. Graduate University of Chinese Academy of Sciences, Beijing 10003., china 3. The center for National Spaceborne Demonstration, Beijing 100101, china 4. Institut National de la Recherche Agronomique, Climat Sol Environnement, 84914 Avignon, France 5. National Satellite Meteorological center, Beijing 100081, china 6. State Environmental Protection Administration, Beijing 10002., china State Key Laboratory of Remote Sensing Science Beijing Normal University Chinese Academy of Sciences Beijing 100101 China Graduate University Chinese Academy of Sciences Beijing 100039 China center for National Spaceborne Demonstration Beijing 100101 China National Satellite Meteorological center Beijing 100081 China Institut National de la Recherche Agronomique Climat Sol Environnement 84914 Avignon France State Environmental Protection Administration Beijing 100029 China

Based on the laboratory calibration before launch of CBERS-02.IRMSS thermal infrared channel, the onboard blackbody calibration, the radiometric cross- calibration against TERRA MODIS corresponding channel and the in-flight field calibration at Lake Qinghai: water surface radiometric calibration test site of china on Aug. 17, 2.04 are carried out in this research. When making onboard blackbody calibration of CBERS-02.IRMSS, it is necessary to understand inside structures and light path in IRMSS camera and receive and process calibration blackbody signals at normal and high temperatures. Onboard blackbody calibration results of each detector are very important to relative radiometric calibration of images processing and absolute radiometric calibration of each detector. In-flight field calibration mainly contains field experiments, obtaining synchronous images, spectrum marching and MODTRAN radiative transmi- ssion computation. Radiometric cross-calibration of CBERS-02.IRMSS thermal band against TERRA MODIS selected 6 times synchronous images of two sensors passing through Lake Qinghai and Lake Taihu from August to December, 2.04 to compute the cross calibration data. Combining the in-flight field calibration and radiometric cross calibration data, the absolute radiometric calibration coefficients are calculated by the linear regression method. This new calibration model can obviously control the radiometric calibration uncertainties aroused by the single point method used in the in-flight calibration of CBERS-02.IRMSS, this kind of sensors cannot receive the cosmic background radiation. In this research, the radiometric calibration coefficients obtained through the linear regression model are 8.5{2.(gain, unit: DN/W m?2.r?1μm?1) and 44.92.(offset, unit: DN).

关键词： CBERS-02 IRMSS thermal band in-flight field calibration onboard blackbody calibration cross calibration.

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Potential control of horseradish peroxidase immobilization on gold electrode

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中国科学：化学英文版 2004年第2期47卷 134-141页

作者： GE Cunwang1,2. YU Wei1, LI Changan1, WANG Nanping2.& GU Ning1 1. Key Laboratory of Molecular and Biomolecular Electronics (Southeast University), Ministry of Education, Research center for Nano-scale Science and Technology, Southeast University, Nanjing 2.0096, china 2. Department of Chemistry, Nantong Teachers College, Nantong 2.6007, china Key Laboratory of Molecular and Biomolecular Electronics (Southeast University) Ministry of Education Research Center for Nano-scale Science and Technology Southeast University Nanjing China Department of Chemistry Nantong Teachers College Nantong China

A new approach based on potential control was firstly used for the immobilization of horseradish peroxidase (HRP) as the model protein. The self-assembly monolayer (SAM) was prepared with 2.aminoethanethiol (AET) on the gold electrode. The charge on HRP was adjusted by means of the acidity of the phosphate buffer solution (PBS) for dissolving the HRP. The in-fluence of electric potential on HRP immobilization was investigated by means of colorimetric immunoassay of enzyme-substrate interaction (CIESI) using an automatic plate reader. The HRP modified electrodes were characterized with X-ray photoelectron spectroscopy (XPS) as well as atomic force microscope (AFM) on template-stripped gold surface. The potential for maximum immobilization of HRP was near the zero charge potential. The result indicates that controlled potential can affect the course of HRP immobilization without the loss of enzymic activity. It is of great significance for the control of biomolecular self-assembly and the intrinsic electric device.

关键词： electric potential control, horseradish peroxidase, immobilization, gold electrode, self-assembly.

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In flight MTF monitoring and compensation for CCD camera on CBERS-02

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Science china(Technological Sciences) 2005年第S2期48卷 29-43页

作者： GU Xingfa1,2.3. LI Xiaoying1,2.4, MIN Xiangjun5, YU Tao1,2. SUN Jijuan5, ZENG Yong1,2.4, XU Hua1,2.& GUO Ding2.6 1. State Key Laboratory of Remote Sensing Science, Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University, Beijing 100101, china 2. The {2. for National Spaceborne Demonstration, Beijing 100101, china 3. Institut National de la Recherche Agronomique, Climat Sol Environnement, 84914 Avignon, France 4. Graduate University of Chinese Academy of Sciences, Beijing 10003., china 5. china {2. for Resource Satellite Data and Application, Beijing 10083., china 6. University of Electronic Science and Technology of china, Chengdu 610054, china State Key Laboratory of Remote Sensing Science Beijing Normal University Chinese Academy of Sciences Beijing 100101 China center for National Spaceborne Demonstration Beijing 100101 China Institut National de la Recherche Agronomique Climat Sol Environnement 84914 Avignon France Graduate University Chinese Academy of Sciences Beijing 100039 China china center for Resource Satellite Data and Application Beijing 100830 China University of Electronic Science and Technology of china Chengdu 610054 China

In this article, the approach of simulating ideal tarp scene was proposed to determine the MTF for CCD camera on CBERS-02. The MTF acquired from this technique was compared to those from some common methods. MTFs achieved from different approaches were employed to compensate the CCD images based on three restoration algorithms: the iterative method, the Wiener filter and the modified inverse filter (MIF). The two-dimensional MTF was established with the value at frequency 0.5 for 45° directional MTF as the value at its four corners. This article addressed the variations of the restored CCD images with different techniques of constructing two-dimensional MTF, restoring algorithms and determining the in-flight MTFs. Results showed images restored with the MTF determined from the technique of simulating ideal tarp scene represent the real scene best. And the restoration technique based on the MIF would produce better restored images than the other two techniques. This research also demonstrated that the two-dimensional MTF used for restoration should be interpolated under the control of the MTF for 45° axis, rather than by multiplying the cross-orbit MTF with along-orbit MTF.

关键词： CBERS-02 CCD MTF the technique of simulating ideal tarp scene.

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Expression and Comparative Analysis of Genes Encoding Outer Membrane Proteins LipL2.,LipL3. and OmpL1 in Epidemic Leptospires

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Acta Biochimica et Biophysica Sinica 2005年第10期37卷 7+650-656页

作者： Xiang-Yan ZHANG~(1#) Yang YU~(2.3.) Ping HE~1 Yi-Xuan ZHANG~(2.3. Bao-Yu HU~1 Yang YANG~1 Yi-Xin NIE~4 Xiu-Gao JIANG~4 Guo-Ping ZHAO~(2.3. Xiao-Kui GUO~(1*) 1 Department of Medical Microbiology and Parasitology,Shanghai Jiaotong University,Shanghai 2.002.,china 2.Research center of Biotechnology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 2.003.,china 3.Chinese National Human Genome center at Shanghai,Zhangjiang High Tech Park,Shanghai 2.12.3.china 4 Institute for Infectious Disease control and Prevention,Chinese center for Disease control and Prevention,Beijing 102.06,china Department of Medical Microbiology and Parasitology Shanghai Jiaotong UniversityShanghai 200025 China Research center of Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Chinese National Human Genome center at Shanghai Zhangjiang High Tech ParkShanghai 201203 China Institute for Infectious Disease control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206 China

Leptospiral outer membrane proteins (OMPs) are highly conserved in different species,andplay an essential role in the development of new immunoprotection and serodiagnosis *** genesencoding LipL2.,LipL3. and OmpL1 were cloned from the complete genome sequence of Leptospirainterrogans serovar lai strain Lai and expressed in *** comparison analysis revealed that the threegenes were highly conserved among distinct epidemic leptospires,including three major epidemic speciesLeptospira interrogans,Leptospira borgpetersenii and Leptospira weilii,in *** analysis wasfurther performed to scrutinize 15 epidemic Leptospira reference strains using the antisera of the *** immunoblot assay and reverse transcription-polymerase chain reaction demonstrated that thesethree OMPs were conservatively expressed in pathogenic *** strains and other pathogenic ***,the use of these recombinant OMPs as antigens in enzyme-linked immunosorbent assay (ELISA)for serodiagnosis of leptospirosis was *** recombinant LipL3. and OmpL1 proteins showed ahigh degree of ELISA reactivity with sera from patients infected with *** strain Lai and otherpathogenic *** results may contribute to the identification of candidates for broad-rangevaccines and immunodiagnostic antigens in further research.

关键词： outer membrane protein expression comparative analysis epidemic leptospire

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Monocular vision-based iterative pose estimation algorithm from corresponding feature points

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Science china(Information Sciences) 2010年第8期53卷 1682-1696页

作者： ZHANG ShiJie 1 , CAO XiBin 1 , ZHANG Fan 1 & HE Liang 2.1 Research center of Satellite Technology, Harbin Institute of Technology, Harbin 150080, china 2.Shanghai {2. {3. Engineering Institute, Shanghai 2.02.3}, china Research center of Satellite Technology Harbin Institute of Technology Harbin China Shanghai aerospace control Engineering Institute Shanghai China

To determine the relative pose between an object and a single camera using 2.-to-3. point correspondences, a kind of iterative methods based on inverse projection ray approach is proposed. An iterative algorithm which is divided into depth recovery stage and absolute orientation stage is also proposed. In the first stage, the optimal translation vector is first computed in terms of rotation matrix via least square method, then the depths of the observed points are estimated by projecting the estimated point orthogonally to the inverse projection ray defined by the image point, and finally 3. points are reconstructed using the estimated depths from previous step. In the second stage, the optimal rotation matrix is estimated by applying Umeyama algorithm to fitting of the 3. model points and 3. estimated points. The above two stages are repeated until the result converges. The global convergence of the two-stage iterative algorithm is proven based on the global convergence theorem. Finally, a spacecraft docking application is implemented to test the effectiveness and convergence of the proposed algorithm by mathematical simulation and physical simulation.

关键词： monocular vision feature point relative pose two-stage iterative algorithm global convergence

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In vitro and in vivo induction of bone formation based on adeno-associ-ated virus-mediated BMP-7 gene therapy using human adipose-derived mesenchymal stem cells

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Acta Pharmacologica Sinica 2007年第6期28卷 839-849页

作者： Yan KANG~2.Wei-ming LIAO~(2.5),Zhen-hua YUAN~4,Pu-yi SHENG~2.Long-juan ZHANG~3.Xiang-wei YUAN~2.Lei LEI~2.2.Department of Orthopedics and ~3.Laboratory of General Surgery,the First Affiliated Hospital,Sun Yat-Sen University,Guangzhou 510080,{2.,~4 National Key Laboratory of Viral Genetic Engineering,National Institute for Viral Disease control and Prevention,Chinese center forDisease control and Prevention,Beijing 100086,{2. Department of Orthopedics the First Affiliated Hospital Sun Yat-Sen University Guangzhou China National Key Laboratory of Viral Genetic Engineering National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China Laboratory of General Surgery the First Affiliated Hospital Sun Yat-Sen University Guangzhou China

Aim:To determine whether adeno-associated virus (AAV)-2.mediated,bone mor-phogenetic protein (BMP)-7-expressing human adipose-derived mesenchymal stemcells (ADMS) cells would induce bone formation in vitro and in ***:ADMS cells were harvested from patients undergoing selective suction-assistedlipectomy and transduced with AAV carrying the human BMP-7 ***-trans-duced cells and cells transduced with AAV serotype 2.carrying the enhancedgreen fluorescence protein gene served as *** cells were qualita-tively assessed for the production of BMP-7 and osteocalcin,and subjected toalkaline phosphatase (ALP) and chinalizarin staining.A total of 2.5~ 106 cellsmixed with type Ⅰ collagen were implanted into the hind limb of severe combinedimmune-deficient (SCID) mice and subjected to a histological analysis 3.weekspost ***:Transfection of the ADMS cells achieved an effi-ciency of 99% at d *** with AAV2.BMP-7 induced the expression ofBMP-7 until d 56,which was markedly increased by d *** cells were positivelystained for *** production and matrix mineralization further con-firmed that these cells differentiated into osteoblasts and induced bone formationin vitro.A histological examination demonstrated that implantation of BMP-7-expressing ADMS cells could induce new bone formation in ***:The present in vitro and in vivo study demonstrated that human ADMS cellswould be a promising source of autologous mesenchymal stem cells for BMPgene therapy and tissue engineering.

关键词： adipose-derived mesenchymal stem ceils adeno-associated virus bone morphogenetic protein gene therapy ectopic bone formation

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Semantic theory of finite lattice-valued propositional logic

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Science china(Information Sciences) 2010年第10期53卷 2022-2031页

作者： PAN XiaoDong 1,2.& XU Yang 2.1 School of Maths, Southwest Jiaotong University, Chengdu 61003., china 2.Intelligent {2. Development center, Southwest Jiaotong University, Chengdu 61003., china School of Maths Southwest Jiaotong University Chengdu China Intelligent control Development center Southwest Jiaotong University Chengdu China

Based on the methodology of Pavelka’s fuzzy propositional logic, in this paper, by combining L-fuzzy set and classical two-value propositional logic theory, a kind of graded semantic theory in lattice-valued propositional logic based on finite lattice implication algebras is established. The notions of L-tautology and L-contradiction are introduced, and several theorems about the relations among different kinds of L-tautologies are given. From the viewpoint of uncertainty reasoning, the notion of satisfiability with certain level for a set of formulas is also defined. On this basis, the semantic consequence operation concerning a set of formulas is proposed, and the properties of semantic consequence operation and the consistency of fuzzy information are discussed.

关键词： lattice-valued propositional logic semantic theory L-tautology consistency lattice implication algebra

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Ecological rebuilding and land reclamation in surface mines in Shanxi Province, china

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Journal of Environmental Sciences 2000年第4期12卷 486-497页

作者： MIAO Ze wei 1, BAI Zhong ke 2. GAO Lin 1 (1. Research center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, china E\|mail: miaozewei@*** 2. Department of Soil Science, Shanxi Agricultural University Taigu, 03 中科院生态环境研究中心北京 1 0 0 0 85 山西师范大学山西临汾 0 3 0 80 1

It is well known that the mining industry produces a large amount of derelict land, and causes pollution of water loss and soil erosion as well as other environmental damage in china. As land is under pressure in china, it is now policy to restore or reclaim land despoiled by mining, the aim being to develop sustainable and healthy arable land ecosystems. This paper outlines the principles and approaches of ecological restoration, which have been adopted in Shanxi Province with reference to three typical surface mines. In the research, the principles of ecological engineering and ecological succession are considered as the critical theories of ecological restoration in mine degraded land. Meanwhile, the paper made a comparative research on main links of ecological rebuilding in three surface mines in Shanxi Province, which include new land construction, treatment of toxic substances, {2. of soil erosion, fertility management, irrigation, ecological planning and the establishment of legislation systems. As the research demonstrated, for successful restoration, new land construction is the fundamental framework, but it must be integrated with ecological engineering including ecological planning, the {2. of soil erosion and vegetation establishment and ecosystem creation in order to optimise land productivity and soil fertility. In addition, the establishment of the legislation systems and organization of administration are also indispensable aspects of ecological rebuilding in mined land.

关键词： ecological restoration land reclamation open-pit mine

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Comparative proteomic analysis of human osteosarcoma and SV40-im-mortalized normal osteoblastic cell lines

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Acta Pharmacologica Sinica 2007年第6期28卷 850-858页

作者： Qian-chen GUO~2.Jing-nan SHEN~(2.5),Song JIN~2.Jin WANG~2.Gang HUANG~2.Long-juan ZHANG~3.Jun-qiang YIN~2.Chang-yeZOU~2.Ming-tao LI~4~2.Department of Orthopedic Surgery,~3.Laboratory {2. of Surgery and ~4 proteomic {2.,Sun Yat-sen University,Guangzhou 510080,china Department of Orthopedic Surgery Sun Yat-sen University Guangzhou China Laboratory center of Surgery Sun Yat-sen University Guangzhou China Proteomic center Sun Yat-sen University Guangzhou China

Aim:Comparative proteomics provide a powerful approach in screening for alter-ations in protein levels and post-translational modifications that are associatedwith *** the present study,we aimed to identify candidate biomarkers todistinguish osteosarcoma (OS) cells from normal osteoblastic ***:Weemployed 3.S cell lines (U2.S,IOR/OS9,and SaOS-2.,and used the SV40-immor-talized normal osteoblastic cell line (hFOB 1.19) as the *** differentialprotein levels in OS and osteoblastic cells were identified using 2.D gel electro-phoresis followed by matrix-assisted laser desorption/ionization-time of flightmass spectrometry *** proteins of interest,the levels of which weresignificantly increased in OS cells,were further characterized by Western ***:Twenty-six proteins were identified,the expression level ofwhich was either significantly increased or decreased in the OS cells as comparedto the control *** expression level of the activator of 90 kDa shock proteinATPase homolog 1 (AHA1),was enhanced 12.4-,2..1-,and 2.0}8-fold in SaOS-2.IOR/OS9,and U2.S cells,respectively,and the level of the stomatin-like protein 2.SLP-2. was increased by 10.4-and 7.8-fold in IOR/OS9 and U2.S cells,respectively,as compared to normal osteoblastic *** observations were confirmed byWestern blot ***:A differential proteomic analysis was suc-cessfully used to identify AHA 1 and SLP-2.that were significantly overproducedin OS cells as compared to normal osteoblastic cells,suggesting that those pro-teins among others may be effective biomarker candidates for the identification ofOS cells.

关键词： AHA1 differentially-produced protein osteosarcoma proteome SLP-2

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Risk analysis model for landslide mass of high slope in dam area

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Science china(Technological Sciences) 2008年第S2期51卷 25-31页

作者： GU ChongShi1,2.HE XianFeng3.4,WU ZhongRu1,2.& SU HuaiZhi1,2.1 College of Water Conservancy and Hydropower Engineering,Hohai University,Nanjing 2.0098,china 2.National Engineering Research {2. of Water Resources Efficient Utilization and Engineering Safety,Nanjing 2.0098,china 3.Yellow River Institute of Hydraulic Research,Zhengzhou 450003.china 4 Embankment Security Defect Prevention and control Engineering Research {2. of MWR,Zhengzhou 450003.china College of Water Conservancy and Hydropower Engineering Hohai University Nanjing China National Engineering Research center of Water Resources Efficient Utilization and Engineering Safety Nanjing China Yellow River Institute of Hydraulic Research Zhengzhou China Embankment Security Defect Prevention and control Engineering Research center of MWR Zhengzhou China

In order to identify the threat of reservoir bank landslide to dam safety and reduce the risk of dam failure,impact factors of the landslide are *** concept of landslide risk degree is put forward to measure the threat of landslide to dam *** monotony of the landslide risk assessment function which changes with different affecting factors is analyzed *** on gene automatic programming,a data mining model of the landslide risk assessment function is constructed combined with impact factors of reservoir bank landslide on dam *** the assessment function of landslide risk has been excavated and established according to practical project information,which provides a valuable reference for analyzing the impact of landslide on dam risk.

关键词： landslide dam failure risk landslide risk

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