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Science china Earth sciences 2010年第5期53卷 700-709页

作者： YU JunBao1,2, LIU JingShuang2, SUN ZhiGao1, SUN WeiDong3, WANG JinDa2, WANG GuoPing2 & CHEN XiaoBing1 1 Key Lab of Coastal Envionment Processed, Yantai institute of Coastal Zone research for Sustainable Development, Chinese academy of sciences, Yantai 264003, china 2 Key Lab of Wetland Ecology and Environment, Northeast institute of Geography and agricultural Ecology, Chinese academy of sciences, Changchun 130012, china 3 Key Laboratory of Isotope Geochronology and Geochemistry, guangzhou institute of Geochemistry, Chinese academy of sciences, guangzhou 510640, china 1. Key Lab of Coastal Envionment Processed Yantai Institute of Coastal Zone Research for Sustainable Development Chinese Academy of Sciences Yantai 264003 China2. Key Lab of Wetland Ecology and Environment Northeast Institute of Geography and Agricultural Ecology Chinese Academy of Sciences Changchun 130012 China3. Key Laboratory of Isotope Geochronology and Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China

Nitrous oxide (N2O) and methane (CH4) emissions were measured using a static chamber method in two adjacent plots of freshwater marsh predominated by Calamagrostis angustifolia, one is seasonal waterlogged (SW) and the other without surface water accumulation (NW), in Sanjiang Plain wetland (47°35′N, 133°31′E), northeast china, during 2002-2004. The diurnal and seasonal flux variations of both gases were significantly correlated with 5-cm-soil temperature. The NW marsh is a source of N2O and sink of CH4, while the SW marsh is sink of N2O and source of CH4. Remarkably, we observed a N2O emission peak under Eh of +300 to +100 mV, and CH4 emission peak under Eh of +300 to +400 mV, which indicate additional sources of N2O and CH4 for the freeze-thaw induced emission peaks of N2O and CH4 observed between late July and early August. Further study shows that the additional N2O and CH4 were emitted under the frozen soil after thawing.

关键词： freeze-thaw region freshwater marsh methane nitrous oxide controlling factors

Physiological Mechanism of High and Stable Yield of No-tillage Cast-transplanted rice

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agricultural sciences in china 2002年第4期1卷 404-409页

作者： LIU Jun, HUANG Qing, FU Hua, LU Xiu-ming, LIU Huai-zhen and LI Kang-huo( rice research institute , guangdong academy of agricultural sciences , guangzhou 510640 , P.R. china) rice research institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 P. R . China

Four years' successive comparative experiments showed that no-tillage cast-transplanted rice (NTCTR), compared with conventional tillage cast-transplanted rice (CK), grew slower and produced less tillers at the early growing stage; but, it had shorter ineffective tillering time, less nutrition consumption, stronger individual growth and more uniform growth between individuals and the colony. These characteristics contribute to the increase not only in the productive tiller percentage but also in the ear quality. Furthermore, the flag leaf of NTCTR had higher photosynthetic rate during the filling stage and no early senescence phenomenon at the late stage, which facilitated the accumulation and the transportation of carbohydrates and improved grain setting rate.

关键词： rice No-tillage cast-transplanted rice(NTCTR) Physiology

Engineering infectious foot-and-mouth disease virus in vivo from a full-length genomic cDNA clone of the A/AKT/58 strain

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Science china(Life sciences) 2009年第2期52卷 155-162页

作者： BAI XingWen1, LI PingHua1, CAO YiMei1, LI Dong1, LU ZengJun1, GUO JianHong1, SUN DeHui1, ZHENG HaiXue2, SUN Pu1, LIU XiangTao1, LUO JianXun1 & LIU ZaiXin1 1 Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory of china, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary research institute of Chinese academy of agricultural sciences, Lanzhou 730046, china 2 Veterinary research institute, guangdong agricultural academy of sciences, guangzhou 510640, china 1. Key Laboratory of Animal Virology of Ministry of Agriculture National Foot-and-Mouth Disease Reference Laboratory of China State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences Lanzhou 730046 China 2. Veterinary research institute Guangdong Agricultural Academy of Sciences Guangzhou 510640 China

Two full-length genomic cDNA clones, pTA/FMDV and pCA/FMDV, were constructed that contained three point-mutants [A174G and A308G (not present in pTA/FMDV); T1029G] in the genome compared with the wild type A/AKT/58 strain of foot-and-mouth disease virus. These two viruses were rescued by co-transfection of pCA/FMDV with pCT7RNAP, which can express T7 RNA polymerase in BHK-21 cell-lines, or by transfection of the in vitro transcribed RNA. Their biological properties were analyzed for their antigenicity, virulence in suckling-mice (LD50) and growth kinetics in BHK-21 cells. The in vivo rescued viruses showed high pathogenicity for 3-day-old unweaned mice (LD50=10?7.5). However, the in vitro transcribed RNA derived from pTA/FMDV had lower pathogenicity for suckling-mice (LD50=10?6), and the in vivo transcribed RNA recovered from pCA/FMDV co-transfected with pCT7RNAP showed no significant differences from the wild type virus. These data showed that recovery of the infectious foot-and-mouth disease virus directly from the use of in vivo techniques was better than from in vitro methods. Furthermore, the reverse genetic procedure technique was simplified to a faster one-step procedure based on co-transfection with pCT7RNAP. These results suggest that in vivo RNA tran- scripts may be more valuable for engineering recombinant foot-and-mouth disease virus than in vitro RNA transcripts, and may contribute to further understanding of the biological properties, such as replication, maturation and quasispecies, of the foot-and-mouth disease virus.

关键词： foot-and-mouth disease virus infectious cDNA clones in vivo transcription A/AKT/58 strain

Studies on high-yielding cultivation of rice in guangdong by diagnosis and control of chlorophyll content and LAI

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rice Science 1991年第3期 4-6页

作者： LIN Xiuzhen HUANG Qiumei TU Zengping rice research institute,guangdong academy of agricultural sciences(GAAS),guangzhou 510640,china

During the 1970’s and 1980’s studies on the rela-tion between LAI and yield had been carried out *** main results were as follows:(1)Usingthe accumulated LAI value at three stages,*** initiation(PI),heading(H)and 20 d afterheading(H),to analyze the relation between LAIand yield was more accurrate than only using theLAI value at H stage(see table);(2)Reaching cer-

关键词： Studies on high-yielding cultivation of rice in guangdong by diagnosis and control of chlorophyll content and LAI high

Studies on high-yielding cultivation of rice in guangdong by diagnosis and control of chlorophyll content and LAI

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Chinese rice research Newsletter 1991年第2期 4-6页

作者： LIN Xiuzhen HUANG Qiumei TU Zengping rice research institute,guangdong academy of agricultural sciences(GAAS),guangzhou 510640,china

关键词： Studies on high-yielding cultivation of rice in guangdong by diagnosis and control of chlorophyll content and LAI high

Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 589-596页

作者： Jian-Zhong YI1, Ming-Qiu LIU1, Cai-Zhu ZHU2, Qiang ZHANG1, Zu-Tian SHENG3, Qing-Yun DU3, Wei-Yao YAN1, and Zhao-Xin ZHENG1* State Key Laboratory of Genetics Engineering, Life Science School, Fudan University, Shanghai 200433, china 1The Veterinary institute of Chinese academy of agricultural sciences, Lanzhou 730046, china 2The Virus research institute of Zhejiang academy of agricultural sciences, Hangzhou 310021, china State Key Laboratory of Genetics Engineering Life Science School Fudan University The Veterinary institute of Chinese academy of agricultural sciences

In this study, two DNA fragments encoding amino acid (141–160)-(21–40)-(141–160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138–160)-(21–40)-(138–160) of the serotype A FMDV were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A. The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine- IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses. FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 μg of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.

关键词： foot-and-mouth disease FMDV serotype O FMDV serotype A VP1 protein bivalent vaccine guinea pig

Potential land for plantation of Jatropha curcas as feedstocks for biodiesel in china

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Science china Earth sciences 2010年第1期53卷 120-127页

作者： WU WeiGuang 1,2,3,HUANG JiKun 1,3 & DENG XiangZheng 1,3 1 institute of Geographic sciences and Natural Resources research,Chinese academy of sciences,Beijing 100101,china 2 Department of Economics and Management,Zhejiang Forestry University,Linan 311300,china 3 Center for Chinese agricultural Policy,Chinese academy of sciences,Beijing 100101,china 1. institute of Geographic sciences and Natural Resources research Chinese Academy of Sciences Beijing 100101 China2. Department of Economics and Management Zhejiang Forestry University Linan 311300 China3. Center for Chinese Agricultural Policy Chinese Academy of Sciences Beijing 100101 China

As a renewable energy,biofuel has attracted great attention in china and the rest of *** with the national food security,china recently has shifted its biofuel development priority from grain-based to non-grain-based biofuels,including forest-based biodiesel,since *** curcas is one of major biodiesel ***,there is rising debate on availability of land for expanding Jatropha curcas *** overall goal of this paper is to evaluate potential land for Jatropha curcas used as feedstock for biodiesel in *** on remote sensing data on land use,data on meteorological,soil and land slope,and suitable environment for Jatropha curcas plantation,this study uses Agro Ecological Zone method and considers social-economic constraints to evaluate potential suitable land for Jatropha curcas plantation in china's major Jatropha curcas production region,Southwest *** results show that while there are some potential lands to expand Jatropha curcas areas,amount of these lands will hardly meet the government's target for Jatropha curcas-based biodiesels development in the *** may need to reconsider its long-term targets on the development of Jatropha curcas-based biodiesels.

关键词： biodiesel Jatropha curcas potential land suitability evaluation Southwest china

Effect of 5'-deletion of Arabi-dopsis profilin2 promoter on its vascular specific expression

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Chinese Science Bulletin 2001年第19期46卷 1627-1630页

作者： LIU Yuhui, WANG Zhixing & JIA ShirongBiotechnology research institute, Chinese academy of agricultural sciences, Beijing 100081, china Biotechnology research institute Chinese Academy of Agricultural Sciences Beijing China

Based on the published sequence of profilin2 promoter of Arabidopsis thaliana, a full-length promoter (1667 bp) was amplified by PCR. The 5’ -end deletion fragments with length of 1380, 1153, 969 and 597 bp were then fused with gus (uid\) gene respectively. Constructed plant expression vectors were individually transferred into Kalan-choe laciniata and transgenic plants regenerated. GUS his-tochemical assay confirmed that the full-length promoter Pfnl.7 was vascular-specific. Deletion assays showed that profilin2 promoter could be divided into three parts. Deletion of fragment 1 ( -1667 -1380 bp) resulted in constitutive expression, suggesting that element(s) responsible for vascular-specific expression might exist in this region. Fragment 2 located at -1153 - -597 bp strongly inhibited gus gene expression. Fragment 3 ( -597 - -1 bp) is considered as a basic domain of profilin2.

关键词： profilin2 promoter Kalanchoe constitutive expression vascular-specific expression.

中国特色农业农村现代化的内涵特征与工程化路径

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农业工程学报 2024年第10期40卷 298-305页

作者：农业农村部规划设计研究院课题组张辉农业农村部规划设计研究院北京100125 不详

中国特色农业农村现代化,是中国式现代化的根基。该研究从历史逻辑、理论逻辑和实践逻辑全面分析了中国特色农业农村现代化的演进逻辑,明确了内涵要义,分析了基本特征,探讨了推进路径,并提出相关政策建议。研究表明,加快中国特色农业农... 详细信息

中国特色农业农村现代化,是中国式现代化的根基。该研究从历史逻辑、理论逻辑和实践逻辑全面分析了中国特色农业农村现代化的演进逻辑,明确了内涵要义,分析了基本特征,探讨了推进路径,并提出相关政策建议。研究表明,加快中国特色农业农村现代化,是中国“三农”历史逻辑的必然选择,是党的“三农”理论逻辑的必然结果,是全面建设社会主义现代化国家实践逻辑的必然要求。从内涵特征分析,中国特色的农业农村现代化,既有农业农村现代化普遍具有的农业竞争力强、乡村多功能性强、城乡融合水平强和支持保护力度强等共同特征,也具有鲜明的国情特征、制度特征、任务特征、时代特征、路径特征和本质特征等中国特色。推进中国特色农业农村现代化,要以工程项目为抓手,实施稳产保供固本工程、乡村产业全链工程、和美乡村建设工程、乡村生态循环工程、乡村文化赋能工程、科技创新攻关工程、农民收入提升工程等。研究可为推动“三农”工作及工程化推进提供理论支撑。

关键词：中国特色农业农村现代化演进逻辑内涵特征工程化路径