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Analysis of gene expression profile induced by EMP-1 in esophageal cancer cells using cDNA Microarray

World Journal of Gastroenterology 2003年第3期9卷 392-398页

作者： Hai-Tao Wang Jian-Ping Kong Fang Ding Xiu-Qin Wang Ming-Rong Wang Lian-Xin Liu Min Wu Zhi-Hua Liu national laboratory of molecular Ontology, Cancer institute, chinese academy of medical science and peking union medical college, beijing 100021, china national laboratory of molecular Oncology Cancer Institute Chinese Academy of Medical Science and Peking Union Medical College Beijing 100021 China.

AIM: To obtain human esophageal cancer cell EC9706 stably expressed epithelial membrane protein-1 (EMP-1) with integrated eukaryotic plasmid harboring the open reading frame (ORF) of human EMP-1, and then to study the mechanism by which EMP-1 exerts its diverse cellular action on cell proliferation and altered gene profile by exploring the effect of ***: The authors first constructed pcDNA3.1/mychis expression vector harboring the ORF of EMP-1 and then transfected it into human esophageal carcinoma cell line EC9706. The positive clones were analyzed by Western blot and RT-PCR. Moreover, the cell growth curve was observed and the cell cycle was checked by FACS technique. Using cDNA microarray technology, the authors compared the gene expression pattern in positive clones with control. To confirm the gene expression profile, semi-quantitative RT-PCR was carried out for 4 of the randomly picked differentially expressed genes. For those differentially expressed genes,classification was performed according to their function and cellular ***: Human EMP-1 gene can be stably expressed in ECg706 cell line transfected with human EMP-1. The authors found the cell growth decreased, among which S phase was arrested and G1 phase was prolonged in the transfected positive clones. By cDNA microarray analysis, 35 genes showed an over 2.0 fold change in expression level after transfection, with 28 genes being consistently up-regulated and 7 genes being down-regulated. Among the classified genes, almost half of the induced genes (13 out of 28 genes) were related to cell signaling, cell communication and particularly to ***: Overexpression of human EMP-1 gene can inhibit the proliferation of EC9706 cell with S phase arrested and G1 phase prolonged. The cDNA microarray analysis suggested that EMP-1 may be one of regulators involved incell signaling, cell communication and adhesion regulators.

关键词：上皮膜蛋白-1 食管癌 Western blot法 RT-PCR 基因表达 cDNA微阵列

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Selenite Induces Oxidative Stress and Apoptosis in Colon Cancer Cells

Selenite Induces Oxidative Stress and Apoptosis in Colon Can...

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2008年全国生物化学与分子生物学学术大会

作者： Fang Huang~1 Quan Chen~2 Cai-min Xu~1 1 national laboratory of medical molecular biology,institute of basic medical sciences,chinese academy of medical sciences and peking union medical college,beijing,china 2 Joint laboratory of Apoptosis and Cancer biology,college of Life sciences,Nankai University,Tianjin 300071,china and The State Key laboratory of Biomembrane and Membrane Biotechnology,institute of Zoology,chinese academy of sciences,beijing 100101,china.

As a chemotherapeutic principle,tumor cells are often sensitive to oxidative stress,there are emerging evidences showing the difference in sensitivity between tumor and normal cells in response to selenite,including p...

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MyoD-regulated long noncoding RNA MCD mediated muscle cell differentiation by regulating myogenin expression via FGF/Erk1/2 signaling pathway

MyoD-regulated long noncoding RNA MCD mediated muscle cell d...

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2012全国发育生物学大会

作者： Xiaohua Yu~*,Fengqi Cao~*,Tingting Li~*,Changyin Li,Ran Zhong,Yuchang Zhou,YongZhang Dahai Zhu national laboratory of medical molecular biology,Department of Biochemistry and molecular biology,institute of basic medical sciences,chinese academy of medical sciences & peking union medical college,5 Dong Dan San Tiao,beijing 100005,PR china.

Although a large number of long non-protein coding RNAs(IncRNAs) have been currently identified in various organisms,functional role of those IncRNAs is largely *** this study we aimed to identify IncRNAs which play determinating role in myogenic cell lineage by bioinformatic analysis with the published data of ChlP-Seq obtained from MEF cells expressing MyoD for discovering MyoD regulated genes and RNA-Seq generated for seeking differentially expressed genes between proliferating and differentiated C2C12 *** discovered 45 IncRNAs which are not only differentially expressed between proliferation and differentiation but also regulated by *** we found that some of the identified IncRNAs are specific to myogenic cell lineage,suggesting unique role of those IncRNAs in regulating developmental program of skeletal *** -Muscle Cell Differentiation(IncR-MCD) is particularly interesting because it meets our scenario in terms of its skeletal muscle-specific and MyoD-regulated expression in C2C12 cells and primary muscle stem cells during ***-MCD is a 447 nucleotide RNA with very lower coding potential and is predominantly localized in *** knocking down or overexpressing of IncR-MCD in C2C12 cells,we showed that IncR-MCD plays critical role in regulating muscle cell *** significance of IncR-MCD in mediating muscle cell differentiation is further corroborated by overexpressing IncR-MCD in MyoD knockdown C2C12 ***-MCD could still be able to initiate muscle cell differentiation in the absence of functional *** significantly,IncR-MCD could functionally convert fibroblasts into myoblasts by regulating myogenin gene expression, uncovering the critical role of an IncRNA in myogenic cell lineage *** further address the underlying mechanism of IncR-MCD action,we revealed that MyoD-regulated IncR-MCD regulates muscle cell differentiation via bFGF-Mek-Erk1/2 signaling pathway. Co

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MicroRNA-128 Inhibits Glioma Cells Proliferation by Targeting Transcription Factor E2F3a

MicroRNA-128 Inhibits Glioma Cells Proliferation by Targetin...

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2008年全国生物化学与分子生物学学术大会

作者： Zhi-qiang Zhao Yuan-gang You Yu Zhang Yan-hua Gong Jian-gang Yuan Xiao-zhong Peng Bo-qin Qiang (national laboratory of medical molecular biology,institute of basic medical sciences,chinese academy of medical sciences and peking union medical college,beijing 100005)

MicroRNAs are～21nt single-stranded RNAs and function as regulators of gene *** stud- ies have shown that microRNAs play crucial roles in tumorigenesis by targeting the mRNAs of oncogenes or tumor *** we show that bra...

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Myostatin induces dermomyotome EMT in somitogenesis

Myostatin induces dermomyotome EMT in somitogenesis

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2012全国发育生物学大会

作者： Yuchang Zhou,Changyin Li,Ran Zhong,Yong Zhang Dahai Zhu national laboratory of medical molecular biology,Department of Biochemistry and molecular biology,institute of basic medical sciences,chinese academy of Mecical sciences andpeking union medical college,5 Dong Dan San Tiao,beijing 100005,PR china.

Myostatin is a potent negative regulator in regulating skeletal muscle *** muscle mass was dramatically increased as evidenced by hyperplasia and hypertrophy in both animals and human due to loss of Myostatin ***,the functional role of Myostatin in early embryonic myogenesis is completely *** this study, we explored myostatin function in regulating embryonic myogenesis by temporospatially examining myostatin expression in both chick and mouse *** found that Myostatin initiates its expression at the centre of dermomyotome,from which muscle progenitors derive,and progresses medially and laterally as developmental *** dynamic expression profiling of Myostatin is highly correlated with the epithelial-mesenchymal transition(EMT) of *** using in ovo electroporation approach,we showed that overexpressing Myostatin promotes EMT of dermomyotome and consistently EMT of dermomyotome is delayed while Myostatin is knocked down with the Myostatin-specific siRNAs, indicating for the first time that Myostatin functions in regulating somitogenesis by promoting EMT of *** functional role of Myostatin in promoting EMT is also supported by treating cancer cells with recombinant Myostatin. Myostatin could induce EMT of A549 and MDCK cancer cells in *** TGF-β1,Myostatin treatment morphologically converts A549 and MDCK cells to mesenchymal cells,which are characterized by downregulated expression of the epithelial markers,such as E-cadherin and ZO-1,and upregulated expression of mesenchymal markers,Vimentin and Snail. Taken together,our data demonstrated that Myostatin could induce EMT both in vitro and in vivo,implying that Myostatin could functionally determine cell fate of muscle progenitors by promoting EMT of dermomyotome to negatively regulate myogenesis during early embryonic myogenesis.

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应用原位双膜法快速筛选人Flt3配体甲醇酵母高表达转化子

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生物化学与生物物理进展 2002年第2期29卷 307-310页

作者：张艳丽陈松森杨克恭狄旭熊安琪张友鸿中国医学科学院基础医学研究所中国协和医科大学基础医学院医学分子生物学国家重点实验室北京100005

原位双膜法是一种基于免疫原理的快速筛选高表达甲醇酵母转化子的方法 ,即首先将固体培养基上的菌落转印至醋酸纤维素薄膜上 ,再利用硝酸纤维素薄膜原位捕获穿过醋酸纤维素薄膜的菌落外泌蛋白 ,然后用免疫方法检测与硝酸纤维素薄膜结合... 详细信息

原位双膜法是一种基于免疫原理的快速筛选高表达甲醇酵母转化子的方法 ,即首先将固体培养基上的菌落转印至醋酸纤维素薄膜上 ,再利用硝酸纤维素薄膜原位捕获穿过醋酸纤维素薄膜的菌落外泌蛋白 ,然后用免疫方法检测与硝酸纤维素薄膜结合的蛋白 .利用此法筛选到人Flt3配体 (hFL)的甲醇酵母高表达转化子 ,液体诱导表达量约 2 0mg L .ELISA结果证明 ,原位双膜法所得的菌落染色强度与该菌落液体诱导表达水平正相关 .蛋白质印迹结果显示 ,培养上清在 2 5ku处有明显杂交条带 ,而对照组杂交呈阴性 ,且表达量随诱导天数增加 .原位双膜法是一种良好的筛选方法 ,可以快捷、准确地筛选高表达酵母转化子 .

关键词：原位双膜法人Flt3配体甲醇酵母高表达转化子表达巴斯德毕赤酵母

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Intergrin gene expression profiles of human hepatocellular carcinoma

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World Journal of Gastroenterology 2002年第4期8卷 631-637页

作者： Lian-Xin Liu Hong-Chi Jiang Wei-Hui Zhang An-Long Zhu Department of Surgery,the First Clinical college,Harbin medical University,Harbin 150001,Heilongjiang Province,china Lian-Xin Liu Zhi-Hua Liu Jing Zhou Xiu-Qin Wang Min Wu national laboratory of molecular Oncology,Department of Cell biology,Cancer institute,chinese academy of medical science & peking union medical college,beijing 100021,china Lian-Xin Liu Hong-Chi Jiang Wei-Hui Zhang An-Long Zhu Department of Surgery the First Clinical College Harbin Medical University Harbin 150001 Heilongjiang Province China Lian-Xin Liu Zhi-Hua Liu Jing Zhou Xiu-Qin Wang Min Wu National Laboratory of Molecular Oncology Department of Cell Biology Cancer Institute Chinese Academy of Medical Science & Peking Union Medical College Beijing 100021 China

AIM: To investigate gene expression profiles of intergringenes in hepatocellular carcinoma (HCC) through theusage of Atlas Human Cancer Array membranes, semi-quantitative reverse transcription polymerase chainreaction (RT-PCR) and Northern ***: Hybridization of cDNA array membrane wasperformed with α 32P-labeled cDNA probes synthesizedfrom RNA isolated from hepatocellular carcinoma andadjacent non-cirrhotic liver. AtlasImage, which is asoftware specific to array, was used to analyze theresult. RT-PCR of 24 pairs specimen and Northern blotof 4 pairs specimen were used to confirm the expressionpattern of some intergrin genes identified by Atlasarrays ***: Among 588 genes spotted in membrane, 17genes were related to intergrin. Four genes were up-regulated, such as intergrin alpha8, beta1, beta7 andbeta8 in HCC. Whereas there were no genes down-regulated in HCC. RT-PCR and Northern blot analysisof intergrin beta1 gene gave results consistent withcDNA array ***: Investigation of these intergrin genesshould help to disclose the molecular mechanism of thecell adhesion, invasive and metastasis of HCC. A fewgenes are reported to have changed in HCC for the firsttime. The quick and high-throughout method of profilinggene expression by cDNA array provides us overviewof key factors that may involved in HCC, and may findthe clue of the study of HCC metastasis and moleculartargets of anti-metastasis therapy. The preciserelationship between the altered genes and HCC is amatter of further investigation.

关键词：肝癌整合素基因表达 RT-PCR 作用机制

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Improving the Prediction of Human MicroRNA Target Genes by Using Ensemble Algorithm

Improving the Prediction of Human MicroRNA Target Genes by U...

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2008年全国生物化学与分子生物学学术大会

作者： Wei Liu Li Zhao Zhi-qiang Zhao Xiao-zhong Peng Jian-gang Yuan Bo-qin Qiang (national laboratory of medical molecular biology,institute of basic medical sciences,chinese academy of medical sciences and peking union medical college,national Human Genome Center,beijing 10000)

MicroRNAs are a class of small endogenous noncoding RNAs which play important regulatory roles mainly by post-transcriptional *** miRNA target genes will help a lot to understand their biological *** developed an ense...

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Human nectin-like 1, a novel membrane protein interacting with protein 4.1 R

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Progress in Natural science:Materials International 2005年第2期 94-97页

作者： YANG Hongbo1, ZHOU Yan1, HU Xiaoyan1, XU Yaqin1, YIN Bin1, ZHU Shengtao1, FAN Wenhong2, FAN Ming2, QIANG Boqin1, YUAN Jiangang1 and PENG Xiaozhong1*(1. national laboratory of medical molecular biology, institute of basic medical sciences, chinese academy of medical sciences, peking union medical college, beijing 100005, china 2. Department of Neurobiology, institute of basic medical science, academy of Military medical sciences, beijing 100850, china) national laboratory of medical molecular biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesPeking Union Medical CollegeBeijing 100005China Department of Neurobiology Institute of Basic Medical ScienceAcademy of Military Medical SciencesBeijing 100850China

Human nectin-like 1 (NECL1) full-length cDNA was cloned by bioinformatics method when searching for candidate membrane proteins interacting with members of protein 4.1 family. The cytoplasmic and extracellular regions of NECL1 were expressed in and purified from ***, and the polyclonal antibody was produced. Interaction between the cytoplasmic region of NECL1 and the 30 kD membrane binding domain of protein 4.1 on red blood cell (4.1R) was demonstrated by IAsys-biosensor system and GST pull-down experiment. Results of biotin-labeled peptide ELISA further demonstrated the key amino acids for the binding. The interaction research of NECL1's cytoplasmic domain provides basis for further study of the functions of NECL1 in nervous system.

关键词： nectin-like 1 protein-protein interaction protein 4.1 R.

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Screening of GJB2 mutations in chinese population

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Journal of Otology 2007年第1期2卷 18-22页

作者： HAN Ming-kun1, HAN Dong-yi1, GUO Yu-fen2, LI Qing-zhong3, ZHAO Ya-li1, 4, RAO Shao-qi1, 5, 6, YUAN Hu1, ZONG Liang1, GUAN Jing2, XU Bai-cheng2, WANG Da-yong1, LAN lan1, WANG Qiu-ju1, 7 1 Department of Otorhinolaryngology/ Head and Neck Surgery, and institute of Otolaryngology, china People Liberation Army General Hospital, beijing, china 2 Department of Otorhinolaryngology/ Head and Neck Surgery, the Second Affiliated Hospital of LanZhou University, Lanzhou, china 3 Deparment of Otlolaryngology, Eye and ENT Hospital of Fudan University, Shanghai, china 4 Department of Biochemistry and molecular biology, institute of basic medical sciences, chinese academy of medical sciences and peking union medical college, beijing, china 5 Departments of Cardiovascular Medicine and molecular Cardiology, the Cleveland Clinic Foundation, Cleveland, Ohio, USA 6 Department of Bioinformatics, Harbin medical University, Harbin, china 7 china national Human Genome Center, beijing, china Department of Otorhinolaryngology/ Head and Neck Surgery and Institute of Otolaryngology China People Liberation Army General Hospital Beijing China Department of Otorhinolaryngology/ Head and Neck Surgery the Second Affiliated Hospital of LanZhou University Lanzhou China Deparment of Otlolaryngology Eye and ENT Hospital of Fudan University Shanghai China Department of Biochemistry and molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China Departments of Cardiovascular Medicine and molecular Cardiology the Cleveland Clinic Foundation Cleveland Ohio USA Department of Bioinformatics Harbin Medical University Harbin China china national Human Genome Center Beijing China

The GJB2 gene(connexin 26) has been shown to be responsible for DFNB1 and DFNA3. We screened the GJB2 gene in 488 patients with prelingual deafness(Group 1), 124 with postlingual deafness(Group 2), and 117 normal hearing subjects(Group 3). We found that, in Group 1, 65 patients(13.32%) were homozygotes or compound heterozygotes and 51 patients (10.45%) carried a single pathogenic mutation. The 235delC mutation was the most frequent mutation, accounting for 73.22% of the known pathogenic alleles in Group 1. No homozygotes or compound heterozygotes were detected in Group 2 or Group 3. Some postlingual deaf patients (2.42%) and normal hearing subjects(4.27%) were 235delC carriers. Our preliminary data indicate that 235delC, the most frequent mutation identified in this study, is a major cause for prelingual deafness.

关键词： prelingual deafness postlingual deafness, GJB2 235delC chinese

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