Myostatin induces dermomyotome EMT in somitogenesis
会议名称：《2012全国发育生物学大会》
会议日期：2012年
学科分类：0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖]
摘 要：Myostatin is a potent negative regulator in regulating skeletal muscle *** muscle mass was dramatically increased as evidenced by hyperplasia and hypertrophy in both animals and human due to loss of Myostatin ***,the functional role of Myostatin in early embryonic myogenesis is completely *** this study, we explored myostatin function in regulating embryonic myogenesis by temporospatially examining myostatin expression in both chick and mouse *** found that Myostatin initiates its expression at the centre of dermomyotome,from which muscle progenitors derive,and progresses medially and laterally as developmental *** dynamic expression profiling of Myostatin is highly correlated with the epithelial-mesenchymal transition(EMT) of *** using in ovo electroporation approach,we showed that overexpressing Myostatin promotes EMT of dermomyotome and consistently EMT of dermomyotome is delayed while Myostatin is knocked down with the Myostatin-specific siRNAs, indicating for the first time that Myostatin functions in regulating somitogenesis by promoting EMT of *** functional role of Myostatin in promoting EMT is also supported by treating cancer cells with recombinant Myostatin. Myostatin could induce EMT of A549 and MDCK cancer cells in *** TGF-β1,Myostatin treatment morphologically converts A549 and MDCK cells to mesenchymal cells,which are characterized by downregulated expression of the epithelial markers,such as E-cadherin and ZO-1,and upregulated expression of mesenchymal markers,Vimentin and Snail. Taken together,our data demonstrated that Myostatin could induce EMT both in vitro and in vivo,implying that Myostatin could functionally determine cell fate of muscle progenitors by promoting EMT of dermomyotome to negatively regulate myogenesis during early embryonic myogenesis.