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Treatment with Vector-expressed Small Hairpin RNAs against Ki67 RNA-induced Cell Growth Inhibition and Apoptosis in Human Renal Carcinoma Cells

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 254-261页

作者： Jun-Nian ZHENG~(1,3*) Ya-Feng SUN~(2#) Dong-Sheng PEI~2 Jun-Jie LIU~1 Teng-Xiang MA~3 Rui-Fa HAN~3 Wang LI~1 Dian-Bao ZHENG~1 Jia-Cun CHEN~1 Xiao-Qing SUN~1 1 Laboratory of urology,Affiliated Hospital of Xuzhou medical College,Xuzhou 221002,china 2 Research Center for Biochemistry and Molecular Biology,Xuzhou medical College,Xuzhou 221002,china 3 institute of urology,medical university of tianjin,tianjin 3002.1,china Laboratory of urology Affiliated Hospital of Xuzhou Medical CollegeXuzhou 221002 China institute of urology Medical University of TianjinTianjin 300211 China Research Center for Biochemistry and Molecular Biology Xuzhou Medical CollegeXuzhou 221002 China

Short hairpin RNAs (shRNAs) transcribed *** polymerase Ⅲ promoters can triggersequence-selective gene silencing in mammalian *** virtue of their excellent function in knocking downexpression of cancer-associated genes,shRNAs could be used as new therapeutic agents for *** of Ki67 in renal cancer has been correlated to a more aggressive tumor phenotype,inhibitionof Ki67 protein expression by means of shRNAs seems to be a promising approach for the therapy of *** this study,we constructed an expression plasmid encoding shRNAs against the Ki67 gene,namedpSilencerKi67,and transfected it into human renal carcinoma *** pSilencerKi67 was shown to signifi-cantly knock down the expression of the Ki67 gene in human renal carcinoma cells,resulting in inhibitingproliferation and inducing apoptotic cell death that can be maintained for at least *** findings offer thepromise of using vector-based shRNAs against Ki67 in renal cancer gene therapy.

关键词： Ki67 gene shRNAs renal cell carcinoma proliferation apoptosis

Inhibition of Proliferation and Induction of Apoptosis in Human Renal Carcinoma Cells by Anti-telomerase Small Interfering RNAs

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 500-506页

作者： Jun-Nian ZHENG~(1,3#*)Ya-Feng SUN~(2#)Dong-Sheng PEI~2 Jun-Jie LIU~1 Jia-Cun CHEN~1 Wang LI~1 Xiao-Qing SUN~1 Qi-Duo SHI~3 Rui-Fa HAN~3 Teng-Xiang MA~3 1 Laboratory of urology,Affiliated Hospital of Xuzhou medical College,Xuzhou 221002,china 2 Research Center for Biochemistry and Molecular Biology,Xuzhou medical College,Xuzhou 221002,china 3 institute of urology,tianjin medical university,tianjin 300070,china Laboratory of urology Affiliated Hospital of Xuzhou Medical CollegeXuzhou 221002 China institute of urology Tianjin Medical UniversityTianjin 300070 China Research Center for Biochemistry and Molecular Biology Xuzhou Medical CollegeXuzhou 221002 China

Telomerase is an attractive molecular target for cancer therapy because it is present in mostmalignant cells but is undetectable in most normal somatic *** telomerase consists of two subunits,an RNA component(hTR)and a human telomerase reverse transcriptase component(hTERT).Smallinterfering RNA(siRNA),one kind of RNA interferences,has been demonstrated to be an effective methodto inhibit target gene expression in human *** investigated the effects of siRNA targeting at both hTRand hTERT mRNA on the inhibition of telomerase activity in human renal carcinoma cells(HRCCs).Theproliferation and apoptosis of HRCCs were *** treatment of HRCCs using hTR and hTERTsiRNAs resulted in significant decrease of hTR mRNA,hTERT mRNA and hTERT *** siRNA canalso inhibit the telomerase activity and the proliferation of ***,they can induce apoptotic celldeath in a dose-dependent *** these findings,we propose that the inhibition of telomerase activityusing siRNA targeting hTR and hTERT might be a rational approach in renal cancer therapy.

关键词： hTERT hTR telomerase siRNA renal cell carcinoma proliferation apoptosis

The mechanism of enterprise-university-esearch institute binding to promote higher education and protection measures——For example tianjin

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学周刊 2012年第23期 29-31页

作者： Xu Zheng (tianjin Polytechnic university TJPU tianjin,china) tianjin Polytechnic university TJPU tianjin china

The Mechanism of Enterprise-university-research institute (EURI) Binding is a market-oriented strategic alliance as the government and industry associations and intermediary organs for auxiliary forces complement, in which enterprises play the leading role and which combines the efforts of enterprises, universities and research institutes. It is the advanced development of enterprise- university-research institute binding cooperation. The development of technology and social economy continue to offer new challenges for higher education. Under this situation, many universities in tianjin area actively develop their superiority and cooperate with enterprise and research department, trying their best to explore various enterprise- university-research institute binding ways to quicken the pace of technology innovation and transfer in order to make the school’s track wider and wider. According to the situation of tianjin area, this text introduces the cases of mechanism of enterprise-university- research binding about this area’s universities and comes up with safeguard measures on the base of analyzing other areas’ mechanism of enterprise-university-research institute binding, combining with educational economy and management theory.

关键词： Higher Education Enterprise-university-research institute Binding Mechanism Safeguard Measure

β→α Transformation of γ-Phase in Sintered WC-Co Cemented Carbides

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Journal of Materials Science & Technology 1996年第5期12卷 398-400页

作者： Shourong LIU (tianjin institute of Hard Alloy tianjin 300222, china)Yi LIU (Shandong university Jinan 250100, china) tianjin institute of Hard Alloy Tianjin 300222 China Shandong university Jinan 250100 China

Varying the morphology and the structure of γ-phase (Co-base Co-W-C solid solution) by means of altering the cooling rate and the preparing method of liquid sintered WC-Co cemented carbides samples, the mechanism of fcc→hcp transformation of γ-phase in WC-Co alloy has been explored. The results show that, the cooling rate is an important affecting factor on fcc→hcp transformation of γ-phase and the fcc→hcp transformation is mainly a diffusive type when cooling WC-Co samples above room temperature

关键词： WC Co Phase in Sintered WC-Co Cemented Carbides Transformation of

Effect of CO_2 laser sclerectomy with iridectomy on ocular hypertension in rabbits

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International Journal of Ophthalmology(English edition) 2011年第1期4卷 30-32页

作者： Zhi-Xiao Xue 1,3,Ying-Xin Li 2,3,Yan-Shan Xu 4,Jiu-Min Yang 1 1 Laser Medicine Laboratory of tianjin medical university,tianjin 300070,china 2 Laser Medicine Laboratory,Biomedical Engineering institute of Chinese Academy of medical Sciences & Peking Union medical College,tianjin 300192,china 3 Laser Medicine Technology Engineering Center of tianjin,tianjin 300192,china 4 Eye Center of tianjin medical university,tianjin 300384,china Laser Medicine Laboratory of tianjin medical university Tianjin 300070 China.

AIM:To evaluate the efficiency and safety of an optimized CO2 Laser glaucoma surgery system for laser sclerectomy with ***:Rabbit trials were performed to evaluate the efficiency and ***:IOP was significantly decreased in laser group compared with trabeculectomy group(P<0.05) from 7th postoperative day to 60th *** with trabeculectomy group,histopathology studies confirmed fewer complications and better effects were found in laser ***:CO2 laser sclerectomy with iridectomy is effective and safe in terms of IOP lowering.

关键词： CO2 laser glaucoma sclerectomy with irid-ectomy intraocular pressure histopathological examination

Transgenic mice designed to express human α-1,2-fucosyltransferase in combination of human DAF and CD59 to avoid xenograft rejection

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Science china(Life Sciences) 2008年第3期51卷 199-204页

作者： LIU BingQian1, CHENG ChuanYu1, WU YuDong1, WEI JinXing1, LI GuangSan2 & MA TengXiang3 1 Department of urology, the First Affiliated Hospital of Zhengzhou university, Zhengzhou 450052, china 2 institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing 100101, china 3 tianjin institute of urology, Second Hospital of tianjin medical university, tianjin 3002.1, china Department of urology the First Affiliated Hospital of Zhengzhou University Zhengzhou China institute of Genetics and Development Biology Chinese Academy of Sciences Beijing China tianjin institute of urology Second Hospital of Tianjin Medical University Tianjin China

The expression of human α-1,2-fucosyltransferase (HT) or complement regulatory proteins has been proved as an strategy to overcome hypercute rejection in discordant xenogeneic organ transplantation. In this study, we examined whether peripheral blood mononuclear cells (PBMCs) from polytransgenic mice expressing the human HT, and complement regulatory proteins (DAF and CD59), can provide more effective protection against xenograft rejection. Transgenic mice were produced by co-injection of gene constructs for human HT, DAF and/or CD59. Flow Cytometry (FCM) was used to screen the positive transgenic mice. PBMCs from transgenic mice were incubated with 15% human serum to evaluate natural antibody binding, complement activation and expression of adhesion molecules. Three transgenes were strongly expressed in PBMCs of transgenic mice, and HT expression signifi- cantly reduced expression of the major xenoepitope galactose-α-1,3-galactose (α-Gal). Functional studies with PBMCs showed that co-expression of HT and DAF or CD59 markedly increased their re- sistance to human serum-mediated cytolysis when compared with single transgenic PBMCs. Moreover, the combined expression of triple transgenes in PBMCs led to the greatest protection against human serum-mediated cytolysis, avoided hyperacute rejection and reduced expression of adhesion mole- cules. Strong co-expression of triple transgenes was completely protected from xenograft hyperacute rejection and partially inhibited acute vascular rejection. The studies suggest that engineering mice to express triple molecules represents an critical step toward prolonging xenograft survival and might be more suitable for xenotransplantation.

关键词： α-1 2-fucosyltransferase complement regulatory proteins galactose-α-1 3-galactose hyperacute rejection acute vascular rejection

Second mode unstable disturbance measurement of hypersonic boundary layer on cone by wavelet transform

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Acta Mechanica Sinica 2011年第4期27卷 488-493页

作者： Jian Han.Nan Jiang.Yan Tian Department of Mathematics,tianjin university,300072 tianjin,china Department of Mechanics,tianjin university,300072 tianjin,china tianjin Key Laboratory of Modern Engineering Mechanics,300072 tianjin,china State Key Laboratory of Nonlinear Mechanics,institute of Mechanics,Chinese Academy of Sciences,100190 Beijing,china Department of Mathematics Tianjin University Tianjin China Department of Mechanics Tianjin University Tianjin China tianjin Key Laboratory of Modern Engineering Mechanics Tianjin China State Key Laboratory of Nonlinear Mechanics Institute of Mechanics Chinese Academy of Sciences Beijing China

Experimental investigation of hypersonic boundary layer instability on a cone is performed at Mach number 6 in a hypersonic wind tunnel. Time series signals of instantaneous fluctuating surface-thermal-flux are measured by Pt-thin-film thermocouple temperature sensors mounted at 28 stations on the cone surface in the streamwise direction to investigate the development of the unstable disturbance. Wavelet transform is employed as a mathematical tool to obtain the multi-scale characteristics of fluctuating surfacethermal-flux both in the temporal and spectrum space. The conditional sampling algorithm using wavelet coefficient as an index is put forward to extract the unstable disturbance waveform from the fluctuating surface-thermal-flux *** generic waveform for the second mode unstable disturbance is obtained by a phase-averaging technique. The development of the unstable disturbance in the streamwise direction is assessed both in the temporal and spectrum space. Our study shows that the local unstable disturbance detection method based on wavelet transformation offers an alternative powerful tool in studying the hypersonic unstable mode of laminar-turbulent transition. It is demonstrated that, at hypersonic speeds, the dominant flow instability is the second mode, which governs the course of laminar-turbulent transition of sharp cone boundary layer.

关键词： Hypersonic boundary layer. Sharp cone Insta-bility Wavelet transform

Influence of p-layer on the performance of n-i-p μc-Si:H thin film solar cells

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Science china(Physics,Mechanics & Astronomy) 2010年第11期53卷 2042-2046页

作者： YUAN YuJie 1,2,ZHANG KaiLiang 1,2,WEI Zhen 1,2 & GENG XinHua 3 1 College of Electronics Information Engineering,tianjin university of Technology,tianjin 300384,china 2 tianjin Key Laboratory of Film Electronic and Communication Devices,tianjin university of Technology,tianjin 300384,china 3 institute of Photo-Electronics Thin Film Devices and Technique,Nankai university,tianjin 300071,china College of Electronics Information Engineering Tianjin University of Technology Tianjin China tianjin Key Laboratory of Film Electronic and Communication Devices Tianjin University of Technology Tianjin China institute of Photo-Electronics Thin Film Devices and Technique Nankai University Tianjin China

The high pressure radio frequency plasma enhanced chemical vapor deposition (RF-PECVD) process was adopted to prepare the n-i-p microcrystalline silicon solar cells,the influence of p-type layers on the performance of the solar cells was investigated,and the optimum p layer suited to the n-i-p microcrystalline silicon solar cells was *** experimental results demonstrate that the performance of the solar cells can be highly affected by the structural and optical properties of the p-layers,and the performance of solar cells can be greatly improved by optimizing p *** have achieved an initial active-area efficiency of 8.17% (V oc =0.49 V,J sc =24.9 mA/cm 2 ,FF=67%) for the μc-Si:H single-junction n-i-p solar cells and an initial active-area efficiency of 10.93% (V oc =1.31 V,J sc =13.09 mA/cm 2 ,FF=64%) for the a-Si:H/μc-Si:H tandem n-i-p solar cells.

关键词： microcrystalline silicon solar cells quantum confinement effect p-type layers

中国政治经济学年度发展报告（2023）（下）

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政治经济学评论 2024年第3期 90+207-208页

作者： Center for Studies of Contemporary Political Economy,Research institute for 21st-Century Marxism,Nankai university·university of Chinese Academy of Social Science Center for Teaching and Research on Capital,Renmin university of china National institute of Socialist Political Economy with Chinese Characteristics,Renmin university of china 南开大学·中国社会科学院大学21世纪马克思主义研究院当代政治经济学研究中心中国人民大学《资本论》教学与研究中心中国人民大学全国中国特色社会主义政治经济学研究中心

编者按2023年，高质量发展扎实推进，新质生产力加快形成，我国经济总体回升向好，圆满实现发展主要预期目标。为针对性地破解经济恢复发展中的难题，聚焦推进中国式现代化进程中的重点、难点问题，中国政治经济学界开展系统深入研究，...

编者按2023年，高质量发展扎实推进，新质生产力加快形成，我国经济总体回升向好，圆满实现发展主要预期目标。为针对性地破解经济恢复发展中的难题，聚焦推进中国式现代化进程中的重点、难点问题，中国政治经济学界开展系统深入研究，取得系列代表性成果。为准确刻画学界研究进展和轨迹，推动中国经济学理论体系的构建和完善，本刊精选议题如期奉上《中国政治经济学年度发展报告（2023）》。自2010年起，本刊连续十四年发布《中国政治经济学年度发展报告》，“年度发展报告”专栏已成为本刊标志性特色栏目。2023年报告聚焦12个专题，分为（上）（下）两篇，其中专题1—6于报告（上）篇刊登，专题7—12由报告（下）篇刊登。2023年报告（下）选题如下：

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