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Hypoxia and inflammatory factor preconditioning enhances the immunosuppressive properties of human umbilical cord mesenchymal stem cells

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World Journal of Stem Cells 2023年第11期15卷 999-1016页

作者： Hang Li Xiao-Qing Ji Shu-Ming Zhang ri-Hui Bi department of Histology and Embryology Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina School of Pharmaceutical science Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina department of Hepatobiliary Surgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Hospital Affiliated to Shanxi Medical UniversityThe Third Hospital of Shanxi Medical UniversityTaiyuan 030002Shanxi ProvinceChina

BACKGROUND Mesenchymal stem cells(MSCs)have great potential for the treatment of various immune diseases due to their unique immunomodulatory ***,MSCs exposed to the harsh inflammatory environment of damaged tissue after intravenous transplantation cannot exert their biological effects,and therefore,their therapeutic efficacy is *** this challenging context,an in vitro preconditioning method is necessary for the development of MSC-based therapies with increased immunomodulatory capacity and transplantation *** To determine whether hypoxia and inflammatory factor preconditioning increases the immunosuppressive properties of MSCs without affecting their biological *** Umbilical cord MSCs(UC-MSCs)were pretreated with hypoxia(2%O_(2))exposure and inflammatory factors(interleukin-1β,tumor necrosis factor-α,interferon-γ)for 24 *** cytometry,polymerase chain reaction,enzyme-linked immunosorbent assay and other experimental methods were used to evaluate the biological characteristics of pretreated UC-MSCs and to determine whether pretreatment affected the immunosuppressive ability of UC-MSCs in coculture with immune *** Pretreatment with hypoxia and inflammatory factors caused UC-MSCs to be elongated but did not affect their viability,proliferation or *** addition,pretreatment significantly decreased the expression of coagulationrelated tissue factors but did not affect the expression of other surface ***,mitochondrial function and integrity were *** pretreatment promoted UC-MSC apoptosis and senescence,it increased the expression of genes and proteins related to immune *** increased peripheral blood mononuclear cell and natural killer(NK)cell proliferation rates and inhibited NK cell-induced toxicity to varying *** In summary,hypoxia and inflammatory factor preconditioning led to higher immunosuppressive effects of MSCs without damaging their b

关键词： Mesenchymal stem cells Umbilical cord Preconditioning Hypoxia,Inflammatory factors Immune regulation

Robust optical flow estimation based on brightness correction fields

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Journal of Zhejiang university-science C(Computers and Electronics) 2011年第12期12卷 1010-1020页

作者： Wei WANG Zhi-xun SU Jin-shan PAN Ye WANG ri-ming SUN School of Mathematical sciences Dalian University of Technology Dalian 116024 China department of Mathematics Harbin Institute of Technology Harbin 150006 China

Optical flow estimation is still an important task in computer vision with many interesting ***,the results obtained by most of the optical flow techniques are affected by motion discontinuities or illumination *** this paper,we introduce a brightness correction field combined with a gradient constancy constraint to reduce the sensibility to brightness changes between images to be *** advantage of this brightness correction field is its simplicity in terms of computational complexity and *** analyzing the deficiencies of the traditional total variation regularization term in weakly textured areas,we also adopt a structure-adaptive regularization based on the robust Huber norm to preserve motion ***,the proposed energy functional isminimized by solving its corresponding Euler-Lagrange equation in a more effective multi-resolution scheme,which integrates the twice downsampling strategy with a support-weight median *** experiments show that our method is more effective and produces more accurate results for optical flow estimation.

关键词： Optical flow field Variational methods Brightness correction fields Median filter Multi-resolution

Telomerase Activity and Telomerase Reverse Transcriptase Expression Induced by Selenium in Rat Hepatocytes

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Biomedical and Environmental sciences 2009年第4期22卷 311-317页

作者： ri-AN YU HUA-JIE CHEN LING-FEI HE BING CHEN XUE-MIN CHEN department of Occupational and Environmental Health School of Public Health Guangdong Pharmaceutical University Guangzhou 510310 Guangdong China department of Occupational and Environmental Health MOE Key Laboratory of Environment and Health School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China department of Dental Medicine The First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou 510080 Guangdong China

Objective To investigate the effects of sodium selenite on telomerase activity, apoptosis and expression of TERT, c-myc and p53 in rat hepatocytes. Methods Selenium at doses of 2.5, 5.0, and 10 μmol/kg was given to SD rats by garage. In rat hepatocytes, telomerase activity was measured by the telomeric repeat amplification protocol （TRAP）, apoptosis was detected by flow cytometry, and expressions of telomerase reverse transcriptase （TERT）, c-myc and p53 were analyzed by reverse transcription-polymerase chain reaction （RT-PCR）. c-Myc and P53 proteins were detected by immunochemistry. Results Selenium at doses of 2.5, 5.0, and 10 μmol/kg significantly increased hepatocellular telomerase activity and induced apoptosis in a dose-dependent manner. Although selenium at doses of 2.5, 5.0, and 10 μmol/kg displayed no obvious enhancing effect on the TERT mRNA expression in rat hepatocytes （P〉0.05）, it significantly increased the c-myc mRNA and p53 mRNA expression at the dose of 10 μmol/kg （P〈0.05）. Selenium at doses of 5.0 and 10 μmol/kg obviously increased the content of P53 protein in rat hepatocytes, but only at the dose of 10 μmol/kg, it significantly promoted the value of c-Myc protein in them. Conclusion Selenium can slightly increase telomerase activity and TERT expression, and significantly induce apoptosis and over-expression of c-myc and p53 at relatively high doses. The beneficial effects of selenium on senescence and aging may be mediated by telomerase activation and expression of TERT, c-myc, and p53 in rat hepatocytes.

关键词： Selenium Telomerase Telomerase reverse transcriptase c-myc p53

Haar wavelet discretization method for free vibration study of laminated composite beam under generalized boundary conditions

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Journal of Ocean Engineering and science 2021年第1期6卷 1-11页

作者： Sung-Ryol So Hoyong Yun Yongho ri Ryongsik O Yong-Il Yun Institute of Advanced science Kim Il Sung UniversityPyongyangDemocratic People’s Republic of Korea Institute of science Kimchaek University of TechnologyPyongyangDemocratic People’s Republic of Korea department of Engineering Machine Pyongyang University of Mechanical EngineeringPyongyangDemocratic People’s Republic of Korea department of Transport Mechanical Engineering Pyongyang University of Mechanical EngineeringPyongyangDemocratic People’s Republic of Korea

Investigation on vibration of laminated composite beam(LCB)is an important issue owing to its wide use as fundamental *** the present work,we study the free vibration of arbitrarily LCB with generalized elastic boundary condition(BC)by using Haar wavelet discretization method(HWDM).Timoshenko beam theory is utilized to model the free vibration of *** LCB is first split into several segments,and then the displacement for each segment is obtained from the Haar wavelet series and their ***’s principle is applied to construct governing equations and the artificial spring boundary technique is adopted to obtain the general elastic boundary and continuity conditions at two ends of *** vibration characteristics of beam with different fiber orientations and lamina numbers is investigated and its displacements are compared with those in previous *** results are shown graphically and demonstrate the validation of our method.

关键词： Laminated beam Haar wavelet discretization method Elastic boundary condition Free vibration Artificial spring boundary technique

Effects of puerarin on burn injury pain mediated by P2X3 receptor

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Effects of puerarin on burn injury pain mediated by P2X3 rec...

Busemann functions on （?）ri（m,n）and（?）IV（n）

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Chang-Shui XU,Jia-ri LIN,Shuang-Mei LIU,Shang-Dong LIANG~* department of Physiology,School of Basic Medical sciences of Nanchang university,Nanchang 330006,China

Objective Peripheral administration of P2X agonists rapidly causes nocifensive behavior in experimental animals and pain sensation in *** of the nociceptive response to peripheral ATP is mediated by P2X *** shows that Puerarin may have anti-nociceptive response and the role of *** effects of Puerarin on rat burn injury pain mediated by P2X receptor was investigated at the present *** Superficial second degree foot/back burn injury models were ***-Dawley rat were randomly divided into sham paw burn group(SPB),puerarin-treated paw unburned control group(PPC),untreated superficial second degree paw burn group(UPB),puerarin-treated superficial second degree paw burn group(PPB),blank paw control group(BPC group),sham back burn group(SBB),puerarin-treated back unburned control group(PBC),untreated superficial second degree back burn group(UBB),puerarin-treated superficial second degree back burn group(PBB) and blank back control group(BBC).Mechanical withdrawal threshold(MWT)and thermal withdrawal latency (TWL)were measured and the expressions of P2X receptor in dorsal root ganglion from test rats were detected by immunohisto-chemistry,RT-PCR,Western blot and in situ *** MWT and TWL in group UPB and PPB were lower than those in group SPB,PPC and BPC(P0.05),and there also was no difference in MWT and TWL between PPC and BPC(P>0.05).The MWT and TWL in group UPB were still decreased,there was difference between group UPB and group SPB(P3 post burn,the expressions of P2X protein and mRNA in dorsal root ganglion from burn injury model rat in group UPB were increased significantly compared with those in other gro

关键词： P2X3 receptor puerarin burn pain

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Progress in Natural science:Materials International 2003年第3期 15-20页

作者： LI Qingzhong , SU Jianbing and JIANG Xiaofa (1. department of Mathematics, Capital Normal university, Beijing 100037, China 2. department of Mathematics, Xuzhou Normal university, Xuzhou 221116, China)

The Busemann function plays a very important role in studying topology and geometry of a complete riemannian mani-fold. In this paper, the Busemann functions on the real classical domain of the first type and the Cart... 详细信息

关键词： Bnsemann function geodesic ray Cartan doroain of the fourth type.

Dynamic hydraulic jump and retrograde sedimentation in an open channel induced by sediment supply: experimental study and SPH simulation

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Journal of Mountain science 2019年第8期16卷 1913-1927页

作者： ZHENG Xiao-gang CHEN ri-dong LUO Min KAZEMI Ehsan LIU Xing-nian State Key Laboratory of Hydraulics and Mountain river Engineering Sichuan UniversityChengdu 610065China Zienkiewicz Centre for Computational Engineering College of EngineeringSwansea UniversitySA1 8ENUK department of Civil and Structural Engineering University of SheffieldSheffield S1 3JDUnited Kingdom

Mountainous torrents often carry large amounts of loose materials into the rivers, thus causing strong sediment transport. Experimentally it was found for the first time that when the intensive sediment motion occurs downstream over a gentle slope, the siltation of the riverbed is induced and the sediment particles can move upstream rapidly in the form of a retrograde sand wave, resulting in a higher water level along the river. To further study the complex mechanisms of this problem, a sediment mass model in the framework of the Smoothed Particle Hydrodynamics(SPH) method was presented to simulate the riverbed evolution, sediment particle motion, and the generation and development of dynamic hydraulic jump under the condition of sufficient sediment supply over a steep slope with varying angles. Because the sediment is not a continuous medium, the marker particle tracking approach was proposed to represent a piece of sediment with a marked sediment particle. The twophase SPH model realizes the interaction between the sediment and fluid by moving the bed boundary particles up and down, so it can reasonably treat the fluid-sediment interfaces with high CPU efficiency. The critical triggering condition of sediment motion, the propagation of the hydraulic jump and the initial siltation position were all systematically studied. The experimental and numerical results revealed the extra disastrous sediment effect in a mountainous flood. The findings will be useful references to the disaster prevention and mitigation in mountainous rivers.

关键词： Dynamic hydraulic jump Retrograde sedimentation Sediment supply Mountainous river Smoothed Particle Hydrodynamics Marked sediment particle

Selective Solid-phase Extraction of Aloe Emodin from Aloe by Molecularly Imprinted Polymers

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Chemical Research in Chinese Universities 2013年第4期29卷 663-666页

作者： TIAN Ming-lei LEE Yu-ri PARK Dong-wha ROW Kyung-ho College of Chemistry and Environmental Engineering Yangtze University Jingzhou 434023 P R. China department of Chemical Engineering Regional Innovation Center for Environmental technology of Thermal Plasma Inha University Ineheon 402-751 Korea

The extraction and separation of aloe emodin were optimized via selective molecularly imprinted solid-phase extraction. Molecularly imprinted polymer was prepared from the functional monomer, methacrylic acid and a mixture of ethanol/dodecanol（90/10, volume ratio） as porogen. It overcomes the common problems of imprin- ting biological polar compounds and shows high selectivity compared favorably with those of non-imprinted polymer and commercially available C18 and silica cartridges in similar aloe emodin tests. Good linearity was obtained be- tween 0.002 and 2.5 mg/mL（r2=0.998） with relative standard deviations below 3.3%.

关键词： Aloe emodin Molecularly imprinted polymer Solid-phase extraction High performance liquid chromato- graphy

Downregulation of EphB2 by RNA interference attenuates glial/fibrotic scar formation and promotes axon growth

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Neural Regeneration Research 2022年第2期17卷 362-369页

作者： Jian Wu Zhen-Yu Zhu Zhi-Wei Fan Ying Chen ri-Yun Yang Yi Li department of Histology and Embryology Medical CollegeNantong UniversityNantongJiangsu ProvinceChina

The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent interaction is a trigger for glial/fibrotic scar formation. In the present study, a new in vitro model was produced by coculture of fibroblasts and astrocytes wounded by scratching to mimic glial/fibrotic scar-like structures using an improved slide system. After treatment with RNAi to downregulate Eph B2, changes in glial/fibrotic scar formation and the growth of VSC4.1 motoneuron axons were examined. Following RNAi treatment, fibroblasts and astrocytes dispersed without forming a glial/fibrotic scar-like structure. Furthermore, the expression levels of neurocan, NG2 and collagen I in the coculture were reduced, and the growth of VSC4.1 motoneuron axons was enhanced. These findings suggest that suppression of Eph B2 expression by RNAi attenuates the formation of a glial/fibrotic scar and promotes axon growth. This study was approved by the Laboratory Animal Ethics Committee of Jiangsu Province, China(approval No. 2019-0506-002) on May 6, 2019.

关键词： astrocyte EphB2 fibroblast glial/fibrotic scar microfluidic platform RNAi spinal cord injury VSC4.1 motoneuron