检索结果-南通市图书馆

Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo

Acta Pharmacologica Sinica 2006年第12期27卷 1608-1615页

作者： Lin LIN~2 Xin FU~2 Xin ZHANG~2 Lian-xu CHEN~2 Ji-ying ZHANG~2 Chang-long YU~(2,4)Kang-tao MA~3 Chun-yan ZHOU~(3,4)2 Institute of Sports Medicine,peking university Third Hospital,beijing 100083,china 3 department of biochemistry and molecular biology,peking university school of basic medical science,beijing 100083,china Institute of Sports Medicine Peking University Third Hospital Beijing 100083 China.

Aim:Bone morphogenetic protein 4(BMP4)is one of the main local contributingfactors in callus formation in the early phase of fracture ***-derivedstromal cells(ADSC)are multipotent *** present study was conducted toinvestigate the osteogenic potential of ADSC when exposed to adenovirus con-taining BMP4 cDNA(Ad-BMP4).Methods:ADSC were harvested from Sprague-Dawley *** exposure to Ad-BMP4,ADSC were assessed by alkaline phos-phatase activity(ALP)assay,RT-PCR and yon Kossa ***4 expressionwas assessed by RT-PCR,immunofluorescence and Western blot analysis,ADSCtransduced with Ad-BMP4 were directly injected into the hind limb muscles ofathyrnic *** Ad-EGFP(enhanced green fluorescence protein)served *** animals were examined by X-ray film and histological ***:The expression of BMP4 was confirmed at both mRNA and protein *** expression of the osteoblastic gene,ALP activity and yon Kossa stainingconfirmed that ADSC transduced with Ad-BMP4 underwent rapid and markedosteoblast differentiation,whereas ADSC transduced with Ad-EGFP and cells leftalone displayed no osteogenic differentiation.X-ray and histological examinationconfirmed new bone formation in athymic mice transplanted with ADSC trans-duced with ***:Our data demonstrated successful osteo-genic differentiation of ADSC transduced with Ad-BMP4 in vitro and in *** may be an ideal source of mesenchyme lineage stem cells for gene therapyand tissue engineering.

关键词： adipose-derived stromal cells BMP4 gene therapy bone formation

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Calcitonin gene-related peptide inhibits interleukin-1β-induced interleukin-8 secretion in human type Ⅱ alveolar epithelial cells

引用

Acta Pharmacologica Sinica 2006年第10期27卷 1340-1345页

作者： Wen-jing LI Teng-ke WANG Xian WANG~2 department of Physiology,school of basic medical sciences,peking university,Key Laboratory of molecular Cardiovascular science,Ministry of Education,beijing 100083,china department of Physiology School of Basic Medical Sciences Peking University Key Laboratory of Molecular Cardiovascular Science Ministry of Education Beijing 100083 China

Aim:Our previous data have shown that type Ⅱ alveolar epithelial(AEⅡ)cellsexpress neuropeptide calcitonin gene-related peptide(CGRP),and that pro-in-flammatory factor interleukin 1-β(IL-1β)induces CGRP secretion in the A549 hu-man AEⅡ cell *** the present study,we investigated the effect of endogenousand exogenous CGRP on IL-1β-induced chemokine interleukin-8(IL-8)***:We used enzyme-linked immunosorbent assay(ELISA)and RT-PCR todetect IL-8 protein and mRNA levels,respectively,siRNA and the stably trans-fected cell line were used to knock down and overexpress the CGRP gene,respectively,and chemiluminescence assay was used to detect reactive oxygenspecies(ROS)***:CGRP-1 receptor antagonist hCGRP8-37(0.1-1nmol·L-1)greatly amplified IL-1β-induced IL-8 *** inhibition of CGRPexpression by siRNA significantly increased IL-8 secretion upon IL-1β ***,cell clones stably transfected with CGRP showed significantly inhibitedmRNA and protein levels of IL-8 induced by 1L-1β.Conclusion:These data implythat AEⅡ cell-derived CGRP suppress IL-1β-induced IL-8 secretion in an autocrine/paracrine *** investigation showed that CGRP attenuated IL-1β-arousedROS formation,which is an early indication of pro-inflammatory factor signaling.

关键词： type Ⅱ alveolar epithelial cells interleukinl interleukin-8 calcitonin gene-related peptide anti-inflammation lung reactive oxygen species

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat

引用

Cell Research 2004年第2期14卷 161-168页

作者： Kai CHEN~(1,*), Dan LI~(1,*), Yu Hui JIANG~1, Wei Juan YAO~1, Xin Juan WANG~2, Xiao Chao WEI~2, Jing GAO~3, Li De XIE~1, Zong Yi YAN~4, Zong Yao WEN~(1**), Shu CHIEN~5 ~1Hemorheology Center, department of Biophysics, school of basic medical sciences, peking university, beijing, 100083, china. ~2department of Biochemical and molecular biology, school of basic medical sciences, peking university, beijing, 100083, china. ~3department of Urology, medical Center, New York university, USA. ~4department of Mechanics and Engineering science, peking university, beijing 100871, china. ~5department of Bioengineering and Whitaker Institute of Biomedical Engineering, university of California, San Diego, La Jolla, CA 92093-0412, USA 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 2department of Biochemical and molecular biology School of Basic Medical Sciences Peking University Beijing 100083 China. 2department of Biochemical and molecular biology School of Basic Medical Sciences Peking University Beijing 100083 China. 3department of Urology Medical Center New York University USA. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 4department of Mechanics and Engineering science Peking University Beijing 100871 China. 1Hemorheology Center Department of Biophysics School of Basic Medical Sciences Peking University Beijing 100083 China. 5department of Bioengineering and Whitaker Institute of Biomedical Engineering University of California San Diego La Jolla CA 92093-0412 USA

The cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) was cloned into RevTet-On, a Tetregulated and high-level gene expression system. The gene expression system was constructed in a human leukemic cell line: Jurkat. By using RevTet-On TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the changes of cellular apoptosis before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the cellular apoptosis ratio was largely dependent on the TRAIL gene expression level. Moreover, it was found that the apoptosis-inducing TRAIL could cause significant changes in the biophysical properties of Jurkat cells. The cell surface charge density decreased, the membrane fluidity declined, the elastic coefficients K_I increased, and the proportion of α-helix in membrane protein secondary structure decreased. Thus, the apoptosis-inducing TRAIL gene caused significant changes on

关键词： TRAIL Tet gene expression system Jurkat apoptosis.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Inhibition of the activating signals in NK92 cells by recombinant GST-sHLAG1α chain

引用

Cell Research 2004年第2期14卷 155-160页

作者： Ai Yu YAO~(1,2), Hai Yang TANG~1, Yun WANG~1, Mei Fu FENG~(1,*), Rou Li ZHOU~(2,*) ~1State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of science, beijing 100080, china ~2department of Cell biology and medical Genetics, school of basic medical sciences, Health science Center, peking university, beijing 100083, china. 2department of Cell biology and medical Genetics School of Basic Medical Sciences Health Science Center Peking University Beijing 100083 China. 1State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Science Beijing 100080 China 1State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Science Beijing 100080 China 1State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Science Beijing 100080 China 2department of Cell biology and medical Genetics School of Basic Medical Sciences Health Science Center Peking University Beijing 100083 China.

The soluble HLA-G1 (sHLA-G1) isoform was found to be secreted by trophoblast cells at the matemo-fetal interface, which suggests that it may act as an immunomodulator during pregnancy. In this paper, we reported that GST-sHLA-G1α chain could bind to its receptor ILT-2 on NK92 cells and then the latter recruited Src homology 2 domaincontaining tyrosine phosphatase-1 (SHP-1), which consequently dephosphorylated some important protein tyrosine kinases and blocked the activation of downstream molecules such as MEK and ERK so that the cytotoxicity of natural killer (NK) cells was inhibited. These results indicated that GST-sHLA-G1α chain might be exploited in new immunotherapy strategies aiming at inducing immunotolerance during allograft, xenograft and autoimmune situations. In addition, we found that modification of O-linked β-N-acetylglucosamine (O-GlcNAc) was involved in NK cells’ activating and inhibitory signals. This may provide a novel molecular target for inducing immunotolerance but needs furthe

关键词： NK92 GST-sHLA-G1α chain signal transduction SHP-1 O-G1cNAc.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Adeno-associated virus-mediated bone morphogenetic protein-7 gene transfer induces C2C12 cell differentiation into osteoblast lineage cells

引用

Acta Pharmacologica Sinica 2005年第8期26卷 963-968页

作者： Min YANG~2, Qing-jun MA~(2,4), Geng-ting DANG~2, Kang-tao MA~3, Ping CHEN~3, Chun-yan ZHOU~(3,4) ~2department of Orthopedics, peking university Third Hospital, beijing 100083, china ~3department of biochemistry and molecular biology, school of basic medical sciences, peking university Health science Center, beijing 100083, china departmentofOrthopedics PekingUniversityThirdHospitalBeijing100083China

Aim: To investigate the effects of bone morphogenetic protein-7 (BMP7)-expressing recombinant adeno-associated virus (AAV) vector on the differentiation of C2C12 cells. Methods: AAV-BMP7 was packaged by infecting the stable cell clone BHK-21 (integrated with recombinant AAV vector plasmid pSNAV-BMP7) with recombinant herpes simplex virus type 1, which expresses AAV-2 Rep and Cap and possesses AAV packaging functions. Following infection with AAV-BMP7 at multiplicities of infection of lx 10～5 vector genomes per cell and subse-quent culture, C2C12 cells were assessed qualitatively for BMP7 production, alka-line phosphatase activity, osteocalcin production and Cbfal and MyoD expression. Results: C2C12 cells transduced with AAV-BMP7 could produce BMP7 protein until d 28. Alkaline phosphatase in the cultured C2C12 cell lysate was elevated. Secreted osteocalcin in the culture medium was detectable at d 12 and Cbfal mRNA expression level was upregulated, coinciding with downregulation of MyoD in a temporal manner. Conclusion: The present in vitro study demonstrated that AAV-BMP7 could infect and efficiently convert C2C12 cells from myoblasts into osteoblast lineage cells.

关键词： C2C12 adeno-associated virus bone morphogenetic protein-7 gene therapy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Recent progress on the structure of Ser/Thr protein phosphatases

引用

science china(Life sciences) 2008年第6期51卷 487-494页

作者： WANG BaiJing1,2, ZHANG Peng1 & WEI Qun1 1 department of biochemistry and molecular biology, beijing Normal university, beijing Key Laboratory, beijing 100875, china 2 school of Life science, Changchun Normal university, Changchun 130032, china department of biochemistry and molecular biology Beijing Normal University Beijing Key Laboratory Beijing China school of Life science Changchun Normal University Changchun China

PP1, PP2A and PP2B, belonging to the PPP family of Ser/Thr protein phosphatases, participate in regulating many important physiological processes, such as cell cycle control, regulation of cell growth and division regulation, etc. The sequence homology between them is relatively high, and ter- tiary structure is conserved. Because of the complexity of the structure of PP2A and the diversity of its regulatory subunits, its structure is less well known than those of PP1 and PP2B. The PP2A holoen- zyme consists of a heterodimeric core enzyme, comprising a scaffolding subunit and a catalytic sub- unit, as well as a variable regulatory subunit. In this study, the subunit compositions, similarities and differences between the Ser/Thr protein phsphatases structures are summarized.

关键词： Ser/Thr phosphatases subunit composition similarities and differences crystal structure

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Experimental Study on the Antitumor Effect of Chicken Anemia Virus vp3 Gene against Liver Carcinoma In Vivo

引用

Journal of Huazhong university of science and Technology(medical sciences) 2003年第2期23卷 105-107,115页

作者：申志发王宇哲宗义强屈伸 department department of biochemistry and molecular biology School of Basic Medical Sciences Tongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030 department of biochemistry and molecular biology School of Basic Medical SciencesTongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030

In order to testify the antitumor effect, especially its effect against liver carcinoma in vivo, of VP3 protein, one kind of protein coded by chicken anemia virus, recombinants pcDNA-vp3 containing chicken anemia virus vp3 gene, and control vector pcDNA3 were mixed with murine liver carcinoma cell lines H22 respectively. The mixture was injected subcutaneously into Balb/C mice. Some days later, the mice were killed and the solid tumor weighed. The antitumor efficiency was evaluated. The manners of VP3 protein in vivo inducing tumor cell death were identified by using TUNEL assay. All the results suggested that the injection of pcDNA-vp3 and H22 mixture resulted in a significant reduction of tumor growth in mice when compared with the results of control groups. TUNEL assay revealed that VP3 induced apoptosis in vivo. All these indicated that CAV vp3 might be a potential new gene in reducing the growth rate of tumor cells in liver carcinoma or in other kind of solid tumors in vivo.

关键词： chicken anemia virus VP3 protein apoptosis gene therapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cloning of Chicken Anemia Virus vp3 Gene and Apoptosis Inductive Effect of vp3 Gene In Vitro

引用

Journal of Huazhong university of science and Technology(medical sciences) 2003年第4期23卷 329-331,334页

作者：孙军王宇哲宗义强屈伸 department of biochemistry and molecular biology

Using PCR technique, the vp3 gene of chicken anemia virus (CAV) was cloned into the eukaryotic expression vector pcDNA3 to construct a recombinant pcDNA vp3. Restriction enzyme digestion and sequencing analysis revealed that CAV vp3 gene was correctly inserted into the blank vector pcDNA3. After LipofectAMINE TM mediated transfection in vitro with pcDNA vp3 and pcDNA3 respectively, the total mRNA was extracted from liver carcinoma cell lines HepG2 and diploid cell line L 02, and RT PCR was performed afterward. The results of RT PCR suggested that vp3 gene was expressed in these two cell lines. At the same time, using in situ apoptotic detection assay, TUNEL kits, the apoptotic cells were found in pcDNA vp3 transfected HepG2, but not in mock transfected cell lines. VP3 could induce cell death by apoptosis in cancer cell lines, but not in diploid cell lines. All the results indicated that CAV vp3 gene, a potential therapeutic agents, has the potential of being used for cancer treatment.

关键词： chicken anemia virus gene cloning apoptosis vp3 gene

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reversal of P-glycoprotein-mediated Multidrug Resistance in SGC7901/VCR Cells by PPARγ Activation by Troglitazone

引用

Journal of Huazhong university of science and Technology(medical sciences) 2010年第3期30卷 326-331页

作者：陈庆周洁蒋春舫陈娟 department of Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and Technology department of biochemistry and molecular biology School of Basic Medical SciencesTongji Medical CollegeHuazhong University of Science and Technology

Over-expression of P-glycoprotein(P-gp),an ATP-dependent drug efflux pump,represents one of the major mechanisms that contribute to multidrug resistance(MDR) in cancer *** study examined the effects of troglitazone,a ligand of peroxisome proliferator-activated receptor gamma(PPARγ),on P-gp-mediated MDR in SGC7901/VCR cells(a vincristine-resistant human gastric cancer cell line).The expression of P-gp was detected by RT-PCR and Western blotting,*** SGC7901/VCR cells were treated with 0.1 mg/L vincristine(VCR) alone or in combination with 1,5,10 μmol/L troglitazone for 24 ***γ was measured by electrophoretic mobility shift assay(EMSA).The intracellular concentration of Rhodamine123(Rh123,a fluorescent P-gp substrate) was assayed to evaluate the activity of *** cell cycle and apoptosis were measured by flow *** results showed that the P-gp was increasingly expressed in SGC7901,BGC823 and SGC7901/VCR cells in turn,suggesting that MDR in the SGC7901/VCR cells was mediated by the increased expression of *** the SGC7901/VCR cells,the expression level of total PPARγ was increased,however,the protein level and activity of PPARγ in the nuclei of cells decreased *** elevated the PPARγ activity in SGC7901/VCR cells in a dose-dependent *** decreased the P-gp expression and markedly enhanced the accumulation of Rh123 in SGC7901/VCR cells in a dose-dependent *** also found that troglitazone significantly increased the percentage of SGC7901/VCR cells in the G2/M phase and decreased the cell percentage in G1 and S phase in a dose-dependent *** significantly increased the apoptotic rate of SGC7901/VCR cells treated by VCR or ADR in a dose-dependent *** was concluded that P-gp-overexpressed SGC7901/VCR cells have minor endogenous PPARγ *** of the PPARγ activity by troglitazone can reverse P-gp-mediated MDR via down-regulating the expression and activity of P-gp i

关键词： multidrug resistance peroxisome proliferator-activated receptor gamma P-glycoprotein troglitazone SGC7901/VCR cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

hhlim基因转录调控区域鉴定及表达调控的初步研究

引用

生物化学与生物物理进展 2002年第6期29卷 910-914页

作者：郑斌温进坤韩梅周爱儒河北医科大学基础医学研究所石家庄050017 河北医科大学基础医学研究所生物化学与分子生物学研究室北京大学医学部生物化学与分子生物学系北京100083

为了研究hhlim基因表达调控机制 ,对该基因 5′上游 - 2 5 37bp序列从 5′端依次进行缺失后 ,利用荧光素酶报告基因检测各种不同长度片段在C2C12细胞中驱动荧光素酶表达的活性 .结果表明 ,在hhlim基因 5′上游- 2 5 37～ - 15 37bp之间... 详细信息

为了研究hhlim基因表达调控机制 ,对该基因 5′上游 - 2 5 37bp序列从 5′端依次进行缺失后 ,利用荧光素酶报告基因检测各种不同长度片段在C2C12细胞中驱动荧光素酶表达的活性 .结果表明 ,在hhlim基因 5′上游- 2 5 37～ - 15 37bp之间存在负调控元件 ,在 - 2 5 3～ - 15 7bp之间含有增强子样序列 .用含有增强子样序列的DNA片段做探针 ,对未分化型和分化型C2C12细胞的核蛋白进行电泳迁移率改变分析 (electrophoreticmopilityshiftassay ,EMSA) .分析的结果显示 ,两种表型细胞中的核蛋白与探针结合所形成滞后带的谱型明显不同 .结果还发现内皮素 1(ET 1)和碱性成纤维细胞生长因子 (bFGF)既能显著诱导C2C12细胞对hhlim的表达 ,也能刺激含增强子样序列的调控区域所驱动的报告基因表达 .提示hhlim基因转录起始点至 - 2 5 37bp的区域内含有负调控元件及增强子样序列 ,该基因的表达受ET

关键词： hhlim基因转录调控区域鉴定表达调控调控序列成肌细胞

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：