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Lymphangiogenic and angiogenic microvessel density in human primary sporadic colorectal carcinoma

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World Journal of Gastroenterology 2008年第1期14卷 101-107页

作者： Ge Yan, Xiao-Yan Zhou, San-Jun Cai, Gui-Hong Zhang, Jun-Jie Peng, Xiang Du, Department of Pathology, Cancer Hospital of Fudan university Department of Oncology, shanghai medical college, Fudan university Colorectal Cancer Center, Fudan university, 270 Dong’an Road, shanghai 200032, china Department of Pathology Cancer Hospital of Fudan University Shanghai 200032 China.

AIM： To investigate the distribution pattern of lymphatic vessels and microvessels in sporadic colorectal carcinoma （SCRC） and their relationship to metastasis and prognosis. METHODS： The lymphatic vessel density （LVD） and microvessel density （MVD） in tumor tissue obtained from 132 patients with primary SCRC, including 74 with metastases and 58 without metastases, were evaluated by immunohistochemistry using antibodies directed against D2-40 and yon Willebrand factor （vWF）. RESULTS：（1） The lymphatic vessels and microvessels at central portions of SCRC often had a reticular architecture with numerous tiny and ill-defined lumina, while those at tumor borders had large and open lumina. The LVD and MVD were both obviously higher in colorectal cancer patients with metastases than in those without （P 〈 0.001）. （2） For each one lymphatic vessel increased, there was a 1.45-fold increase in the risk of metastasis in SCRC. The specificity and sensitivity of LVD in predicting metastasis or non-metastasis in SCRC were 71.62% and 56.90%, respectively, and the corresponding LVD was 5. For each one microvessel increased, there was a 1.11-fold increase in the risk of metastasis in SCRC. The specificity and sensitivity of MVD were 66.22% and 51.72%, respectively. （3） Double labeling immunohistochemistry showed D2-40 immunoreactivity to be specific for lymphatic vessels. （4） Univariate analysis indicated that high LVD, high MVD, as well as co-accounting of high LVD and high MVD were associated with patient＇s poor disease-free survival （Puni 〈 0.05）; multivariate analysis indicated that co-accounting of LVD and MVD was an independent prognostic factor of colorectal cancer, CONCLUSION： D2-40 is a new specific antibody for lymphatic endothelial cells. Lymphogenesis and angiogenesis are commonly seen in SCRC, especially at tumor borders. The detection of LVD and MVD at tumor borders may be useful in predicting metastasis and prognosis in patients with SCRC, and, in particular, coaccount

关键词： Lymphangiogenesis Angiogenesis Lymphaticvessel density Microvessel density Sporadic colorectalcarcinoma Metastasis

Stereoselectivity of epidermal carboxylesterase metabolism as observed in Ha-CaT keratinocytes

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Stereoselectivity of epidermal carboxylesterase metabolism a...

第十五届国际药理学大会

作者： Quan-Gang ZHU Jin-Hong HU Ji-Yong LIU Jin-Li Jing-Wang(Department of pharmacy Changhai hospital second military medical university shanghai 200433 china)

＜正＞ Aim: To study stereoselectivity and molecular mechanism of epidermal car-boxylesterase metabolism as observed in HaCaT keratinocytes. Methods: Keto-profen ethyl ester was used as a model drug, and HaCaT cell homogenates was applied for studying the stereoselectivity of carboxylesterase metabolism. The concentrations of all samples were assayed by HPLC. Human liver L02 cell strain was used as control of carboxylesterase expression, and RT - PCR was used for studying the expression of carboxylesterase. Results: The main metabolite of ke-toprofen ethyl ester in HaCaT cell homogenates was R - ketoprofen. Human craboxylesterase （hCE） - 2 was highly expressed in HaCaT keratinocytes. However, the expression of hCE- 1 was very weak or not detectable. Conclusion: hCE - 2 is more abundant carboxylesterase in HaCaT keratinocytes that may be responsible for stereoselective hydrolysis of ketoprofen ethyl esters. This pilot study reinforces the methods of improving transdermal absorption by prodrugs.

关键词： carboxylesterase HaCaT cell line ketoprofen ethyl ester stereose-lectivity

CXCL16 participates in pathogenesis of immunological liver injury by regulating T lymphocyte infiltration in liver tissue

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World Journal of Gastroenterology 2005年第32期11卷 4979-4985页

作者： Huan-Bin Xu, Yan-Ping Gong, Jin Cheng, Yi-Wei Chu, Si-Dong Xiong, Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, shanghai medical college of Fudan university Immunology Division, E-Institute of shanghai Universities, shanghai 200032, china Department of Immunology Shanghai Medical College of Fudan University Shanghai 200032 China.

AIM： To investigate the role of CXCL16 in the pathogenesis of immunological liver injury and to explore the possible mechanism ofT lymphocyte infiltration regulated by CXCL16. METHODS： Immunological liver injury in murine model was induced by Bacille Calmette-Guerin and lipopolysaccharide. Expression pattern and distribution of CXCL16 were examined by real-time quantitative RT-PCR and immunohistochemical analysis. Anti-CXCL16 antibody was administrated in vivo to investigate its effect on T-cell recruitment and acute hepatic necrosis. The survival of murine model was also evaluated. RESULTS, The murine immunological liver injury model was successfully established, CXCL16 expression increased and predominantly distributed in periportal areas and vascular endothelia in injured liver tissues. Administration of anti-CXCL16 Ab protected the mice from death and acute liver damage. Approximately 70% of the mice survived for 72 h in the anti-CXCL16 Ab treatment group, whereas 80% died within 72 h in control Ab group. The number of liver-infiltrating T lymphocytes was significantly reduced from 1.01×10^7 to 3.52×10^6/liver, compared with control Ab treatment. CONCLUSION： CXCL16 is involved in immunological liver injury by regulating T lymphocyte infiltration in liver tissue.

关键词： Chemokines CXCL16 T lymphocytes Infiltration Immunological liver injury

Effect of heparin on apoptosis in human nasopharyngeal carcinoma CNE2 cells

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Cell Research 2001年第4期11卷 311-315页

作者： LI HONG LIANG , KAI HE YE , HAI WEI ZHANG , YING RU LUO , XIAN DA REN, AI HUA XIONG, RUI SITU Department of Pharmacology ,pharmacy college, Department of Pathology medical college, Jinan university, Guangzhou 510632, china Department of Pharmacology Pharmacy College Jinan University Guangzhou China Department of Pathology medical college Jinan University Guangzhou China

In order to study the mechanism of the effect of heparin on apoptosis in carcinoma cells, the nasopha- ryngeal carcinoma cell line CNE2 was used to identify the effect of heparin on apoptosis associated with the expression of c-myc,bax, bcl-2 proteins by use of Hoechst 33258 staining, terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling (TUNEL), agarose gel electrophoresis, and flow cytometry, as well as Western blot analysis. The results showed that heparin induced apoptosis of cNE2 cens including the morphologic changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the 'ladder pattern' revealed by agarose gel electrophoresis of DNA in a concentration-dependent manner. The number of TUNEL-positive cells was dramatically increased to 33.6+1.2% from 2.8+0.3% by treat- ment with heparin in different concentrations (10~40 kU/L). The apoptotic index was increased to 32.5% from 3.5% by detecting SubG1 peaks on flow cytometry. Western blot analysis showed that levels of bcl-2, bax and c-myc were significantly overexpressed by treatment with the increase of heparin concentrations. These results suggest that heparin induces apoptosis of CNE2 cells, which may be regulated by differential expression of apoptosis-related genes.

关键词： Heparin agarose gel electrophoresis flow cytometry apoptosis protein kinase C(PKC)

Synthesis and Structural Characterization of One-dimensional Polynuclear Complex [La(bipyN_2O_2)(TFA)_3]_n

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Chinese Chemical Letters 2000年第10期11卷 935-936页

作者： Wen Xiang ZHU, Yun HE (Department of Chemistry, Beijing Normal university, Beijing 100875) (Department of pharmacy, Beijing Staff medical college, Beijing 100036) Department of Chemistry Beijing Normal University Beijing 100875 China Department of pharmacy Beijing Staff Medical College Beijing 100036 China

A novel polymeric complex [La(bipyN2O2)(TFA)3]n(TFA= trifluoroacetylacetone) was prepared. The X-ray structure analysis revealed that the complex has an infinite one-dimensional supramolecule structure with the 4,4'-bipyridyl-N,N'-dioxide as a bridge. La(III) is coordinated to eight oxygen atoms, six from three trifluoroacetylacetonate anions, two from two 4,4'-bipyridyl-N,N'-dioxide molecules.

关键词： Lanthanum polynuclear complex trifluoroacetylacetone 4,4'-bipyridyl-N,N'-dioxide.

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Effect of matrine on the expression of substance P receptor and inflammatory cytokines production in human skin keratinocytes and fibroblasts

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Effect of matrine on the expression of substance P receptor ...

中华中医药学会中药实验药理分会第七届学术会议

作者： Ji-Yong LIU Jin-Hong HU~* Peng-Cheng Quan-Gang ZHU Feng-Qian LI, Department of pharmacy,Changhai hospital,second military medical university, (shanghai 200433 china)

objective:The present study was designed to investigate the effect of matrine on the expression of neurokinin 1 receptor(NK-1R) and cytokines production induced by substance P (SP) in HaCaT cells and dermal ***:HaCaT ...

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Methyl jasmonate-induced lithospermic acid B and rosmarinic acid based on related gene transcription and metabolites accumulation profiling in Salvia miltiorrhiza hairy root cultures

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Methyl jasmonate-induced lithospermic acid B and rosmarinic ...

第二届上海市植物生理学青年学术研讨会

作者： Ying Xiao, Wansheng Chen *Department of pharmacy, Changzheng Hospital, second military medical university, shanghai 200003

＜正＞This work is to examine the lithospermic acid B （LAB） and rosmarinic acid （RA） accumulation, as well as related gene transcript and metabolite profiling of Salvia miltiorrhiza

关键词： Salvia miltiorrhiza lithospermic acid B (LAB) rosmarinic acid (RA) methyl jasmonate (MeJA) transcript and metabolite profiling antioxidant activities.

A New Phthalide,Graveolide from Apium graveolens Seeds

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A New Phthalide,Graveolide from Apium graveolens Seeds

中国医药教育协会成人教育委员会三届五次理事大会暨医药教育创新研究和慢病防治学术研讨会

作者： Li Ming QIAO~1,Hai Sheng CHEN~(2*),Run Hui LIU~3,Ting Zhao LI~4,Shuang LIANG~5 (Depatment of Natural Medicinal Chemistry,college of pharmacy, second military medical university,shanghai 200433 )

Aim To investigate the chemical constituents from the seeds of Apium *** The EtOAc fraction from the ethanol extraction of the seeds of Apium Graveolens were isolated by column chromatography with silica gel and Sephadex *** structures of the chemical constituents were determined by means of spectroscopic *** Six phthalide compounds were isolated,and their structures were elucidated as 3-n-buty 1-4,5-dihydrophthalide,ligustilide,neocnidilide,3 -n-Butyl Hexahydrophthalide,3- n-Buthl phthalide,and 3-butyl-4,5-dihydro-6β,7β-dihydroxyphthalid named Graveolide by chemical and spectroscopic *** Compound 1,3-butyl-4,5-dihydro - 6β,7β- dihydroxyphthalid was a new phthalide compound named *** 2-6 were the known phthalide compounds.

关键词： Apium graveolens Umbelliferae phthalides 3-butyl-4,5- dihydro - 6β,7β-dihydroxyphthalid Graveolide

Correlative study on the cognitive dysfunction of patients with myasthenia gravis

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Neural Regeneration Research 2006年第8期1卷 744-746页

作者： Wenli Chen1, Wenmin Wang2, Yi Wang3 1Department of Neurology, Yunnan First People’s Hospital, Kunming 650032, Yunnan Province, china 2Department of Neurology, First Affiliated Hospital of Kunming medical college, Kunming 650032, Yunnan Province, china 3Department of Neurology, Huashan Hospital Affiliated to shanghai Fudan university, shanghai 200040, china Department of Neurology Yunnan First People's Hospital Kunming 650032 Yunnan Province China Department of Neurology Hospital of Kunming Medical College Kunming 650032 Yunnan Province China Department of Neurology Huashan Hospital Shanghai Fudan University Shanghai 200040 China

BACKGROUND: Myasthenia gravis (MG) is considered as a peripheral neuromuscular disorder. Some investigations demonstrate that MG affects central nervous system (CNS), and there are disorders in cognitive function, affection, sleep, mental status and others; however, there are also some different standpoints. Some questions are still disputed, such as whether or not MG exists in the CNS and cognitive function is impaired. DATA SOURCES: Using the terms of 'myasthenia gravis, neuropsychological test, memory, central, brain, cognitive dysfunction and so on', we searched the Medline database for the articles about CNS involved in MG, nerve and neuropsychology as well as cognitive function, which were published in the English language between January 1985 and December 2004. Meanwhile, we also searched CNKI database by inputting 'myasthenia gravis, neuropsychology and cognitive function' in Chinese for articles about involved neuropsychology and cognitive function which were published between October 1995 and June 2006 in the Chinese language. STUDY SELECTION: Data were checked in the first trial, and articles about the influences of MG on CNS, neuropsychology, cognitive function and central cholinergic function were selected, then those were obviously unrelated with above criteria were excluded. Articles which expound the influences of MG on neuropsychology and cognitive were included, but those with repeated contents were also excluded. DATA EXTRACTION: A total of 89 related English articles and 34 Chinese articles were collected, including 43 studies on involved CNS and 32 studies on involved central cholinergic function and cholinergic receptor. Sixty-three literatures were about neuropsychology and cognitive function, which were closely related with this article. Seventy-two articles met inclusive criteria and 66 were excluded. Thirty of seventy-two were chosen for analysis. DATA SYNTHESIS: MG is an autoimmune disease of muscular diseased fatigue induced by transmissi

关键词： cognitive function central nervous system cholinergic receptor autoimmune disease English language Chinese language used in

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