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Two-photon spectroscopic behaviors and photodynamic effect on the BEL-7402 cancer cells of the new chlorophyll photosensitizer

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Science china Chemistry 2008年第6期51卷 529-538页

作者： ZHAO PeiDe1,2,CHEN Ping1,TANG GuoQing1,LIU JinWei2,LIN Lie1,GUO Peng1,YU Qing2, YAO JianZhong3,MA DongMing4,ZHANG GuiLan1 & CHEN WenJu1 1 Key Laboratory of Optoelectronic Information Science and Technology,Ministry of Education,Institute of Modern Optics,Nankai university,Tianjin 300071,china 2 Department of Physics,Hebei university of Technology,Tianjin 300130,china 3 college of pharmacy,second military medical university,shanghai 200433,china 4 Tianjin college of the Traditional Chinese Medicine,Tianjin 300193,china Key Laboratory of Optoelectronic Information Science and Technology Ministry of Education Institute of Modern Optics Nankai University Tianjin China Department of Physics Hebei University of Technology Tianjin China college of pharmacy Second Military Medical University Shanghai China Tianjin college of the Traditional Chinese Medicine Tianjin China

The spectroscopic properties of a new chlorophyll derivate photosensitizer (CDP) are studied under the excitation wavelengths at 800 and 400 nm using femtosecond pulses from a Ti: sapphire laser. The damaging effect of CDP on the BEL-7402 cancer cells is also investigated upon two-photon illumination at 800 nm. The normalized fluorescence spectra of CDP in tetrahydrofuran (THF) show that two-photon and one-photon spectra have the same distributions and the same emission bands (675 nm). The lifetimes of two- and one-photon induced fluorescence of this molecule are of the order of 5.0 ns. By comparing the data it is shown that there is some difference between the two lifetimes, but the difference is less than one nanosecond. The two-photon absorption cross section of the molecule is also measured at 800 nm and estimated as about σ'_2 ≈ 31.5x10^(-50)cm^4·s·photon^(-1). The results of two-photon photodynamic therapy (TPPDT) tests show that CDP can kill all of the tested cancer cells according to the usual Eosine assessment. Our results indicate that the two-photon-induced photophysical, photochemical and photosensitizing processes of CDP may be basically similar to those of one-photon excitation. These behaviors of the sample suggest that one may find other possible methods to estimate some photosensitizers' effects in details such as their distribution in cells and the reactive targets of the sub-cellular parts of some tumor cells via two-photon excitation techniques.

关键词： photosensitizer chlorophyll derivate two-photon fluorescence two-photon absorption cross-section TPPDT BEL-7402 cell

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Protective effect of steroidal saponins from rhizome of Anemarrhena asphodeloides on ovariectomy-induced bone loss in rats

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Acta Pharmacologica Sinica 2006年第6期27卷 728-734页

作者： Hua NIAN~2 Lu-ping QIN~2 Wan-sheng CHEN~3 Qiao-yan ZHANG~(2,4)Han-chen ZHENG~2 Yin WANG~5 2 Department of Pharmacognosy,School of pharmacy,second military medical university,shanghai 200433.china 3 Department of pharmacy,Changzheng Hospital,second military medical university,shanghai 200003,china 5 № 455 Hospital of PLA,shanghai 200052,china Department of Pharmacognosy School of Pharmacy Second Military Medical University Shanghai China Department of pharmacy Changzheng Hospital Second Military Medical University Shanghai China No 455 Hospital of PLA Shanghai China

Aim:To investigate the protective effect of steroidal saponins from Anemarrhenaasphodeloides（ATS）on ovariectomy（OVX）-induced bone ***:Sprague-Dawley rats were divided into sham and OVX *** OVX ratswere treated with vehicle,nylestriol or steroidal saponins extract for 12 *** calcium,phosphorus,estradiol（E2）,osteocalcin concentration and serumalkaline phosphatase activity were *** density was assayed by dual-energy X-ray *** undecalcified longitudinal proximal tibial meta-physical（PTM）sections were cut and stained for histomorphometric analysis ofthe ***:In OVX rats,alkaline phosphatase activities in serum weremarkedly increased and concentrations of osteocalcin were decreased by ATStreatment,which had no influence on the body ***,atrophy of theuterus and descent of bone mineral density（BMD）was suppressed by treatmentwith *** addition,ATS completely corrected the decreased the concentrationof calcium and E2in serum observed in OVX *** results showedATS prevented decreases in trabecular thickness and increases in trabecular sepa-ration of proximal tibia metaphysis（PTM）in OVX ***,it did not alterosteoclast number in OVX ***,ATS（300 mg/kg）had a remarkableeffect on promoting bone formation action in OVX *** treatmentdecreased the bone formation rate and mineral apposition ***:Anadequate supply of steroidal saponins of Anemarrhena asphodeloides preventedOVX-induced bone loss in rats through the promotion of bone formation but notthe inhibition of bone resorption.

关键词： Anemarrhena asphodeloides ovariectomy osteoporosis serum bone mineral density histochemistry

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Effects of esculentoside A on production of interleukin-1, 2, and prostaglandin E2

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Acta Pharmacologica Sinica 2004年第6期25卷 115-119页

作者： Zhen-yu XIAO, Qin-yue ZHENG, Yuan-ying JIANG, Bin ZHOU, Ming YIN2, Hong-bin WANG, Jun-ping ZHANG3 Department of Pharmacology,second military medical university, shanghai200433 2college of pharmacy,Jiao Tong university, shanghai 200030, china Department of Pharmacology Second Military Medical University 上海 200433 上海交通大学上海 200030

AIM: To investigate the influence of esculentoside A (EsA) on immunological function and its mechanism of anti- inflammation. METHODS: Interleukin-1 production was measured by thymocyte co-stimulating assay; the radio- activity of [3H]arachidonic acid (AA) was used to evaluate the release of AA; prostaglandin E2 production was measured with radioimmunoassay (RIA); IL-2 and IFN-γ were detected by ELISA method. RESULTS: EsA (3-12 μmol/L)could potently inhibit the production of IL-1 and PGE2 from both silent and LPS induced macrophages. EsA had no significant effect on the release of AA from murine macrophages. EsA could inhibit the production of IL-2 from murine lymphocytes induced by ConA, but not affect the production from silent lymphocytes. EsA showed no effect on the production of IFN-γ from both silent and ConA induced lymphocytes. CONCLUSION: EsA could affect the immunological function through inhibiting the production of IL-2 from activated splenocytes and the inhibition of production of IL-1 and PGE2 might be one of the anti-inflammation mechanisms of EsA.

关键词： esculentoside A interleukin-1 interleukin-2 prostaglandins E interferon-gamma

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A new phenolic compound from Crinum asiaticum L.

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Chinese Chemical Letters 2008年第4期19卷 447-449页

作者： Qian Sun~a Wei Dong Zhang~(a,b)Yun Heng Shen~(a,*)Chuan Zhang~(a,*)Hui Liang Li~a ~aDepartment of Phytochemistry,School of pharmacy,second military medical university,shanghai 200433,china ~bSchool of pharmacy,shanghai Jiao tong university,shanghai 200030,china Department of Phytochemistry School of Pharmacy Second Military Medical University Shanghai 200433 China School of pharmacy Shanghai Jiao tong University Shanghai 200030 China

A new phenolic compound was isolated from the ethanol extract of the bulbs of Crinum asiaticum *** *** structure was defined as 1-(2-hydroxy-4-hydroxymethyl)phenyl-6-O-caffeoyl-β-D-gluco-pyranoside on the basis of sp... 详细信息

关键词： Crinum asiaticum L.var.sinicum baker Amaryllideae Phenolic compound 1-(2-Hydroxy-4-hydroxymethyl)phenyl-6-O-caffeoyl-β-D-glucopyranoside

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Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract

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Acta Pharmacologica Sinica 2005年第7期26卷 835-839页

作者： Ya-bin JIAO~2 Yao-cheng RUI~3 Tie-jun LI Peng-yuan YANG Yan QIU Department of Pharmacology,School of pharmacy,the second military medical university,shanghai 200433,china 2 Now in Department of Pharmacology,School of pharmacy,shanghai university of Traditional Chinese Medieine,shanghai 201203,china. Department of Pharmacology School of Pharmacy the Second Military Medical University Shanghai China

Aim:To study the protein and mRNA expressions of pro-inflammatory and anti-inflammatory cytokines in the brain of rats with atherosclerosis(AS)and theeffects of Ginkgo biloba extract(GbE)on expressions of ***:The expermental model of AS in rats were established by intraperitioneal injectionof vitamin D3 with high fat/cholesterol *** 100 mg/kg was administered torats by *** 8 weeks,the expressions of IL-1β,TNF-α,IL-10,and 1L-10R in thebrain tissues of AS rats were detected by enzyme-linked immunosorbant assay,immunohistochemistry,Western blotting,and reverse transcriptase polymerasechain ***:The protein and mRNA expressions of IL-1β,TNF-α,andIL-10 in the brains were markedly higher in AS groups than that in control groups(6.11±0.15,1.55±0.14,0.54±0.04 ng/g wet weight vs 0.80±0.14,0.33±0.09,and0.33±0.02 ng/g wet weight,respectively).The protein and mRNA expres-sions of IL-1β and TNF-α in the brains were markedly lower in GbE groups(3.82±0.54,0.95±0.08 ng/g wet weight)than that in AS groups,the protein andmRNA expressions of IL-10 and 1L-10R in the brains were markedly higher in GbEgroups(0.85±0.06 ng/g wet weight)than that in AS ***:GbEinhibited production of pro-inflammatory cytokines IL-1β and TNF-α,but up-regulated the production of anti-inflammatory cytokines,1L-10 and IL-10R in brain,which might be related with its anti-AS actions.

关键词： atherosclerosis cytokine interleukin-1 tumor necrosis factor interleukin-10 Ginkgo biloba rats

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Synergism of hydrochlorothiazide and nitrendipine on reduction of blood pressure and blood pressure variability in spontaneously hypertensive rats

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Acta Pharmacologica Sinica 2006年第12期27卷 1575-1579页

作者： Ping HAN Zheng-xu CHU Fu-ming SHEN He-hui XIE Ding-feng SU~2 Department of Pharmacology,School of pharmacy,second military medical university,shanghai 200433,china Department of Pharmacology School of Pharmacy Second Military Medical University Shanghai China

Aim:To investigate the possible synergism of hydrochlorothiazide andnitrendipine on reducing both blood pressure(BP)and blood pressure variabil-ity(BPV)in spontaneously hypertensive rats(SHR).Methods:Seventy ani-mals were randomly divided into seven *** doses were 5 and 10 mg/kgfor nitrendipine,10 and 20 mg/kg for hydrochlorothiazide and 10+5,20+10mg/kg,respectively,for the combination of these two drugs and 0.8% carboxym-ethylcellulose as *** drugs were given via a catheter of gastric *** was then continuously recorded for 5h from 1h before drug administrationto the end of 4th hour after drug administration,in conscious and freely ***:The effects on both BP and BPV reduction of the combination ofhydrochlorothiazide and nitrendipine were greater than the single drug in *** two drugs possessed an obvious synergism on both systolic blood pressure(q=1.79 with small dose and q=1.23 with large dose)and systolic blood pres-sure variability reduction(q=1.79 with small dose and q=1.39 with large dose)in ***:The present work clearly demonstrated that there was asynergistic effect between hydrochlorothiazide and nitrendipine in lowering andstabilizing BP in SHR.

关键词： blood pressure blood pressure variability hydrochlorothiazide hypertension nitrendipine spontaneously hypertensive rat

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Effects of Changtai granules, a traditional compound Chinese medicine, on chronic trinitrobenzene sulfonic acid-induced colitis in rats

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World Journal of Gastroenterology 2005年第23期11卷 3539-3543页

作者： Yong-Bing Cao,Jun-Dong Zhang,Lan Yan,De-Jun Wang,Xin-Ming Jia,Ping-Hui Gao,Ming-He Cheng,Zheng Xu,Yan Wang,Yuan-Ying Jiang,Department of Pharmacology,School of pharmacy,second military medical university,shanghai 200433,china Ya-Ying Diao,Department of pharmacy,Changhai Hospital,second military medical university,shanghai 200433,china Department of Pharmacology School of Pharmacy Second Military Medical University Guohe Road 325 Shanghai 200433 China.

AIM: To study the effects of Changtai granules (CTG), a traditional compound Chinese medicine, on chronic trinitrobenzene sulfonic acid-induced colitis in rats. METHODS: Healthy adult Sprague-Dawley (SD) rats of both sexes, weighing 250-300 g, were employed in the present study. The rat colitis models were induced by 2, 4,6-trinitrobenzene sulfonic acid (TNBS) enemas at a concentration of 100 mg/kg in 50% ethanol. The experimental animals were randomly divided into dexamethasone (DX) treatment, CTG treatment, and model control groups, which were intracolicly treated daily with DX (0.2 mg/kg), CTG at doses of 2.9, 5.7 and 11.4 g crude drug/kg, and the equal amount of saline respectively from 6 h following induction of the colitis in rats inflicted with TNBS to the end of study. A normal control group of rats treated without TNBS but saline enema was also included in the study. After 3 wk of treatment, the animals were assessed for colonal inflammatory and ulcerative responses with respect to mortality, frequency of diarrhea, histology and myeloperoxidase activity (MPO).RESULTS: The therapeutic effect of CTG on ulcerative colitis (UC) was better than DX. CTG effectively inhibited the activity of granulocytes, macrophages and monocytes in a dosedependent manner. Also it reduced MPO and formation of inflammation in colonic mucosal tissue. Furthermore, administration of CTG significantly prevented body mass loss and death, and decreased frequency of diarrhea in UC rats, when compared with the model control group ***: CTG would prove to be an ideal drug for chronic UC, and is warranted to be studied further.

关键词： Ulcerative colitis Changtai granules 2,4,6-Trinitrobenzene sulfonic acid Myeloperoxidase

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Effects of combination therapy with atenolol and amlodipine on blood pressure control and stroke prevention in stroke-prone spontaneously hypertensive rats

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Acta Pharmacologica Sinica 2007年第11期28卷 1755-1760页

作者： Gang LING,Ai-jun LIU,Fu-ming SHEN,Guo-jun CAI,Jian-guo LIU,Ding-feng SU~2Department of Pharmacology,School of pharmacy second military medical university,shanghai 200433,china Department of Pharmacology School of Pharmacy Second Military Medical University Shanghai China

Aim:To test the effects of atenolol and amlodipine,either alone or in combination,on blood pressure,blood pressure variability (BPV),baroreflex sensitivity (BRS),and the prevalence of stroke in stroke-prone spontaneously hypertensive rats(SHR-SP).Methods:In the first set of the study,24 8-month-old,female SHR-SPrats were randomly divided into 3 *** pressure,heart period,and BRSwere determined before and after the intragastric administration of atenolol (10mg/kg) and amlodipine (1.0 mg/kg),either alone or in *** the secondset of the study,40 male and 40 female rats were randomly assigned to 1 of thefollowing 4 groups:control,atenolol (10 mg·kg-1·d-1),amlodipine (1.0 mg·kg-1·d-1),and both (10 male and 10 female in each group).The stroke incident and survivaltime were ***:Atenolol and amlodipine,either alone or incombination,significantly decreased blood pressure,with the exception of theamlodipine-induced effect on diastolic blood ***,only the com-bination treatment significantly decreased the BPV levels for the same *** q-values calculated by the probability sum analysis were 1.17 and 2.67 forsystolic and diastolic blood pressure,respectively,and were 2.48 and 2.10 forsystolic and diastolic BPV,respectively,following *** drugexhibited any significant effect on *** and amlodipine,either alone orin combination,significantly increased the lifespan of SHR-SP,with the best ef-fect elicited by the combination ***:A significant synergismexists between atenolol and amlodipine in lowering and stabilizing blood pressurein *** therapy may be an optimal strategy for the prevention ofstroke in hypertension.

关键词： stroke hypertension stroke-prone spontaneouslyhypertensive rats atenolol amlodipine blood pressure blood pressure variability

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Effects of CpG-ODN on gene expression in formation of foam cells

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Acta Pharmacologica Sinica 2005年第11期26卷 1359-1364页

作者： Kai LI, Bin WAN, Zhen-lin HU, Ying HE, Xiao-wen HE, Lei JIANG, Shu-han SUN~2 Department of medical Genetics, college of Basic medical Sciences, the second military medical university, shanghai 200433, china Department of medical Genetics College of Basic Medical Sciences the Second Military Medical University Shanghai China

Aim: To investigate the effects of CpG-oligodeoxynucleotide (CpG-ODN) on the formation of macrophage foam cells and related gene expression. Methods: A gene expression profile was examined by microarray techniques, and mRNA expression was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The cholesterol and cholesteryl ester contents of cells were determined by high performance liquid chromatography. Results: CD36, LPL, and Fcγ2b, which were related to lipid metabolism and the formation of macrophage foam cells, were upregulated after CpG-ODN stimulation. The mRNA expression related to the formation of foam cells was confirmed by semiquantitative RT-PCR. Moreover, histochemical analysis confirmed that lipid deposits inside cells increased after CpG-ODN treatment. However, using flow cytometry, we found that CpG-ODN had no effect on the expression of membrane receptors. Conclusion: CpG-ODN up-regulated the expression of genes in macrophage foam cell formation.

关键词： CpG islands oligodeoxyribonucleotides foam cells macrophages LDL lipoprotein gene expression cholesterol CD 36 antigens lipoprotein lipase Fc gamma receptor Ⅱb

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Inhibition of ATP-induced calcium influx in HT4 cells by glucocorticoids:involvement of protein kinase A

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Acta Pharmacologica Sinica 2005年第2期26卷 199-204页

作者： Jian-zhong HAN,Wen LIN~2,Yi-zhang CHEN~3Neuroscience Research Institute and Department of Neurobiology,~2Department of Pharmacology,college of pharmacy,second military medicaluniversity,shanghai 200433,china Neuroscience Research Institute and Department of Neurobiology College of Pharmacy Second Military Medical University Shanghai China Department of Pharmacology College of Pharmacy Second Military Medical University Shanghai China

Aim:In our previous observations,adenosine triphosphate(ATP)was found toevoke immediate elevations in intracellular free calcium concentration([Ca2+]i)in HT4 neuroblastoma cells of *** tried to see if a brief pretreatment ofglucocorticoids could inhibit the Ca2+response and reveal the underlying signal-ing ***:Measurement of[Ca2+]iwas carried out using thedual-wavelength fluorescence method with Fura-2 as the ***:Pre-incubation of HT4 cells for 5 min with corticosterone(B)or bovine serum albu-min conjugated corticosterone(B-BSA)inhibited the peak[Ca2+]iincrements in aconcentration-dependent *** and dexamethasone had a similar action,while deoxycorticosterone and cholesterol were *** extracellularCa2+influx and intemal Ca2+release contributed to ATP-induced[Ca2+]*** brief treatment with only B attenuated Ca2+***,the[Ca2+]ielevation induced by the P2X receptor agonist adenosine 5’-(β,γ-methylene)triph-osphate(β,γ-meATP)was also *** rapid inhibitory effect of B canbe reproduced by forskolin 1 mmol/L and blocked by H89 20 mmol/*** glucocorticoid receptor antagonist mifepristone nor protein kinase C in-hibitors influenced the rapid action of ***:Our results suggest thatglucocorticoids modulate P2X receptor-medicated Ca2+influx through a mem-brane-initiated,non-genomic and PKA-dependent pathway in HT4 cells.

关键词： calcium glucocorticoids adenosine triphosphate protein kinase C protein kinase A

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