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Ectopic expression of a hyacinth AGL6 homolog caused earlier flowering and homeotic conversion in Arabidopsis

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Science china(Life Sciences) 2007年第5期50卷 676-689页

作者： FAN JinHui1, LI WenQing2, DONG XiuChun1, GUO Wei1 & SHU HuaiRui3 1 college of forestry, shandong agricultural university, taian 271018, china 2 college of Biological Science, china agricultural university, Beijing 100094, china 3 college of Horticulture Science and Engineering, shandong agricultural university, taian 271018, china college of forestry Shandong Agricultural University Taian 271018 China college of Biological Science China Agricultural University Beijing 100094 China college of Horticulture Science and Engineering Shandong Agricultural University Taian 271018 China

MADS-box genes are involved in floral organ *** we report thatan AGL6(Agamous-like 6)-like MADS-box gene,H0AGL6,was isolated from Hyacinthus *** pattern analysis demonstrated that H0AGL6 transcript was detected in inflorescencebuds,tepals,carpels and ovules,but not in stamina,leaves or *** Arabidopsis plantsectopically expressing H0AGL6 exhibited novel phenotypes of significantly reduced plantsize,extremely early flowering,and losing inflorescence *** addition,wide homeoticconversion of sepals,petals,and leaves into carpel-like or ovary structures,and disappearance ornumber reduction of stamens in 35S::HoAGL6 Arabidopsis plants were also ***-PCR analysisindicated that the expressions of flowering time gene SOC1 and flower meristem identity gene LFYwere significantly up-regulated in 35S::Ho4GL6transgenic Arabidopsis plants,and the expressionlevels of floral organ identity genes AG and SEP1 in leaves were also *** resultsindicated that H0AGL6 was involved in the regulation of flower transition and flower organformation.

关键词： AGL6 homologue, flower development, flowering time, MADS-box genes, Hyacinthus orientalis

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石榴疮痂病病原的初步研究

山东农业大学学报（自然科学版） 2001年第4期32卷 26-26页

作者：刘会香刘振宇杜明芸王先炜靳勇谢玉石义华山东农业大学林学院山东泰安271018 枣庄市薛城林业局山东枣庄277000 枣庄市薛城区南常乡林业站山东枣庄277000

石榴是世界上栽培较早的果树之一,主要分布在亚热带及温带地区.我国南至广东、广西、云南、贵州,北至山东、山西、河南、河北,东到江苏、浙江及安徽等省,西至四川、陕西、甘肃及新疆等地,均有分布[1].枣庄是我省的石榴主栽区,目前已发... 详细信息

石榴是世界上栽培较早的果树之一,主要分布在亚热带及温带地区.我国南至广东、广西、云南、贵州,北至山东、山西、河南、河北,东到江苏、浙江及安徽等省,西至四川、陕西、甘肃及新疆等地,均有分布[1].枣庄是我省的石榴主栽区,目前已发展近万亩,成为当地农民脱贫致富的一条好路子,占当地农民总收入的一半以上,为当地的支柱产业.但近年来病害严重发生,制约着石榴产业的发展,据报道,影响我国石榴发展的主要病害有:石榴疮痂病、石榴干腐病、石榴褐斑病.石榴疮痂病是石榴上一种严重的枝干和果实病害,目前,国内外对该病害研究较少,且病原不一,为澄清该病害的病原,了解其发生发展规律,为防治提供科学理论依据,我们自1996年起,对该病害的病原做了系统的研究,现报道如下:1材料与方法

关键词：石榴疮痂病病原菌分离致病性鉴定

Immobilization of an enzyme from a Fusarium fungus WZ-I for chlorpyrifos degradation

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Journal of Environmental Sciences 2010年第12期22卷 1930-1935页

作者： Hui Xie,Lusheng Zhu,Tingting Ma,Jun Wang,Jinhua Wang,Jun Su,Bo Shao college of Resources and Environment,shandong agricultural university,taian 271018,china college of Resources and Environment Shandong Agricultural University Taian 271018 China

The free enzyme extracted from WZ-I, which was identified as Fusarium LK. ex Fx, could effectively degrade chlorpyrifos, an organophosphate insecticide. The methods of immobilizing this free enzyme and determined its degradation-related characteristics were investigated. The properties of the immobilized enzyme were compared with those of the free enzyme. The optimal immobilization of the enzyme was achieved in a solution of 30 g/L sodium alginate at 4℃ for 4-12 hr. The immobilized enzyme showed the maximal activity at pH 8.0, 45℃. The maximum initial rate and the substrate concentration of the immobilized enzyme were less than that of the free enzyme. The immobilized enzyme, therefore, had a higher capacity to withstand a broader range of temperatures and pH conditions than the free enzyme. With varying pH and temperatures, the immobilized enzyme was more active than the free enzyme in the degradation reaction. In addition, the immobilized enzyme exhibited only a slight loss in its initial activity, even after three repeated uses. The results showed that the immobilized enzyme was more resistant to different environmental conditions, suggesting that it was viable for future practical use.

关键词： organophosphate pesticide immobilized enzyme degradation fungus

Sustainable Intensification and Large-scale Operation of Cultivated Land Use at the Farmers’ Scale:A Case Study of shandong Province,china

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Chinese Geographical Science 2024年第1期34卷 149-167页

作者： LI Li LYU Xiao ZHANG Anlu NIU shandong PENG Wenlong School of Public Administration Huazhong Agricultural UniversityWuhan430070China Department of Geography University of ZurichZurich8057Switzerland School of Humanities and Law Northeastern UniversityShenyang110169China School of Geography and Tourism Qufu Normal UniversityRizhao276800China

Sustainable intensification of cultivated land use(SICLU) and large-scale operations(LSO) are widely acknowledged strategies for enhancing agricultural ***,the existing literature has faced challenges in precisely defining SICLU and constructing comprehensive indicators,which has hindered the exploration of factors influencing LSO within the SICLU *** address this gap,we integrated self-efficacy theory into the design of an index framework for evaluating *** subsequently employed econometric models to analyze the significant factors that impact *** findings reveal that SICLU can be divided into four key dimensions:intensive management,efficient output,resource conservation,and ecological environment ***,it is crucial to incorporate belief-based cognitive factors into the index system,as farmers’ understanding of fertilizer and pesticide application significantly influences their willingness to engage in ***,we identify grain market turnover as the most influential factor in promoting LSO,with single-factor contribution rates reaching 70.9% for cultivated land transfer willingness and 62.5% for the total planting ***,unlike irrigation and agricultural machinery inputs,increased labor inputs correspond to larger planting areas for *** trend may be attributed to reduced labor availability because of rural labor migration,whereas the reduction in irrigation and agricultural input is contingent on innovations in production practices and the transfer of cultivated land management ***,SICLU dynamically influences LSO,with each index related to SICLU having an optimal range that fosters *** insights offer valuable guidance for policymakers,emphasizing farmers as their central focus,with the adjustment of input and output factors as a means to achieve LSO as the ultimate *** conclusion,we propose research avenues for further enriching the SICLU framework to ensur

关键词： sustainable intensification of cultivated land use(SICLU) self-efficacy status quo bias input and output Boosted Regression Tree willingness to transfer cultivated land cultivated land planting areas shandong,china

Ultrastructural Alteration of Maize Plants Infected with the Maize Rough Dwarf Virus

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agricultural Sciences in china 2002年第5期1卷 531-534页

作者： LI Zhao-hui, GUO Xing-qi, YE Bao-hua and GUO Yan-kui( college of Plant Protection , shandong agricultural university, taian 271018 , P.R.china college of Life Sciences , shandong agricultural university , taian 271018 , P.R.china) college of Plant Protection Shandong Agricultural University Taian 271018 P.R. China college of Life Sciences Shandong Agricultural University Taian 271018 P. R. China

The ultrastructural alteration of maize plants infected with the maize rough dwarf virus (MRDV) was studied with transmission electron microscopy. The results revealed that aggregates of virus particles , with a diameter of 60nm, were found in the root cell, and always distributed near the vacuole membrane. However, no such particles were checked in leaf cells. Moreover, no virus was observed in choroplasts, mitochondria nuclei, plasmodesmata or intercellular canal of all kinds of infected cells of maize, either. Structures of various organelles changed in the infected leaf and root cells of maize. An inward collapse and localized splitting of the tonoplast were observed, the chloropoast structure was destroyed by MRDV, and the number of destroyed or dysplasia chloroplast in leaf cells with serious symptoms was more than that in leaves without symptoms. The matrix of mitochondria in cells infected by MRDV decreased and some of them expanded and destructed. Nuclei was abnormal and the nuclear membrane was broken. In addition, the infected cells were characterized by a voluminous cytoplasm containing hypertrophied endoplasmic reticulum, with rich ribosome content and lots of starch grain.

关键词： Maize rough dwarf virus Cy topathology Electron microscopy

Apoptosis in Granulosa cells during follicular atresia: relationship with steroids and insulin-like growth factors

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Cell Research 2004年第4期14卷 341-346页

作者： Yuan Song YU, Hong Shu SUI, Zheng Bin HAN, Wei LI, Ming Jiu LUO, Jing He TAN Laboratory of Animal Reproduction and Embryology college of Animal Sciences and Veterinary Medicine, shandong agricultural university, taian, shandong 271018, china Present address: college of life science. university of Beijing, Beijing, china. Laboratory of Animal Reproduction and Embryology college of Animal Sciences and Veterinary Medicine Shandong Agricultural University Taian China college of life science University of Beijing Beijing China

It is well known that during mammalian ovarian follicular development, the majority of follicles undergo atresia at various stages of their development. However, the mechanisms controlling this selection process remain unknown. In this study, we investigated apoptosis in granulosa cells during goat follicular atresia by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The changes in the levels of steroids, insulin-like growth factors (IGFs) and IGF receptors were studied by radioimmunoassay (RIA) and semi-quantitative reverse transcrip-tion-PCR. We found that the percentage of apoptotic granulosa cells in the atretic (A) follicles was significantly higher than that in the slightly atretic (SA) and healthy (H) follicles. The level of estradiol and the ratio of estradiol to progesterone in H follicles were significantly higher than those in A follicles. On the other hand, the level of progesterone was not significantly different among these follicle types. We also found that t

关键词： apoptosis ovarian follicle steroids IGFs atresia.

Effect of Different Fertilizing Methods on Zinc Nutrition in Zn-Deficiency Apple Trees

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agricultural Sciences in china 2002年第12期1卷 1365-1369页

作者： WANG Yan-an, FAN Wei-guo, ZHANG Fang-ai, LI Ling and LI De-quan(college of Science and Technology, shandong agricultural university, taian 271018 ,P. R.china college of Life Science , shandong agricultural university , taian 271018 , P. R. china) college of Science and Technology Shandong Agricultural University Taian 271018P.R.China college of Life Science Shandong Agricultural University Taian 271018 P.R.China

Red Fuji apple trees of Zn-deficiency were used as material in the experiment. The effect of different Zn application methods on the regularity of Zn transportation and nutrition was studied. It was found that the storage of Zn in the branches was greatly increased by foliar application of the solution of 15% ZnSO4·7H2O before abscission. It had advantage to keep suitable Zn content in leaves, branches and roots, as well as between Zn and P and K, which increased Zn transportation and reusability. Zn content in roots increased when Zn was used in soil in autumn. However, the absorbed Zn did not transport to the top of the plant. It was used by the plant next year. Foliar application of ZnSO4·7H2O at early stage of the plant growth enhanced Zn content of the branch. However, the effect was kept for a short time. It had little effect on Zn transportation when Zn was sprayed three weeks after blossoming.

关键词： Apple trees Zinc Nutrition balance Foliar application

Effects of Soil Aeration on Sweet Potato Yield and Its Physiological Mechanism

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agricultural Sciences in china 2002年第2期1卷 189-194页

作者： SHI Chun-yu,WANG Zhen-lin and YU Song-lie(college of Agronomy, shandong agricultural university, taian 271018) college of Agronomy Shandong Agricultural University Taian 271018

The effects of soil aeration on physiological characters and root tuber yield of Ipomoea batatas (L.) Lam. CV Lushu7 and Xushu18 were studied. The results showed that soil aeration improvement could increase ATP content and ATPase activity in functional leaves and root tubers and ABA content in root tubers. It also accelerated the transportation of 14C-photosynthate from leaves to root tubers and enhanced dry matter distribution in root tubers and thus root tuber yield was significantly raised. The role of ATP, ATPase and ABA in accelerating the transportation of 14C-photosynthate was discussed based on the changes of soluble carbonhydrate content in sweet potato plant.

关键词： Sweet potato Soil aeration Root tuber yield Physiological peculiarity

Study on the Pathogenicity of Chinese Strains of Subgroup J Avian Leukosis Viruses

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agricultural Sciences in china 2002年第5期1卷 585-588页

作者： DU Yan and GUI Zhi-zhong(college of Animal Science and Veterinary Medicine, shandong agricultural university, taian 271018 , P.R. china) college of Animal Science and Veterinary Medicine Shandong Agricultural University Taian 271018 P. R. China

The pathogenicity of 4 Chinese strains of subgroup J avian leukosis viruses (ALV-J), SD9901, SD9902, YZ9901 and YZ9902,was studied. The results showed that only SD9902 among the 4 strains induced mortality from myeloid leukosis (ML). In the 12 meat-type chickens inoculated with SD9902 at 1-day-old, 9 died between 22 days and 38 days after inoculation. No death or ML was found in chickens inoculated with the other 3 strains during the period of 6 months. These results suggested the SD9902 strain of ALV-J was an acute transforming virus, but SD9901, YZ9901 and YZ9902 were non-transforming viruses. All 4 Chinese strains did not induce any tumors in egg-type SPF chickens during 7 months after hatching when viruses were injected into 11-day-old embryos.

关键词： ALV-J Pathogenicity Myeloid leukosis