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Urotensin Ⅱ accelerates cardiac fibrosis and hypertrophy of rats induced by isoproterenol

Acta Pharmacologica Sinica 2007年第1期28卷 36-43页

作者： Yong-gang ZHANG~(2,5) Yu-guang LI~2 Bao-guo LIU~3 Rui-hong WEI~2 Dong-ming WANG~2 Xue-rui TAN~2 Ding-fang BU~4 Yong-zheng PANG~4 Chao-shu TANG~42 Department of cardiovascular Diseases,first affiliated hospital,Shantou university medical College,Shantou 515041,china 3 the affiliated hospital,medical school of hebei university of engineering,hebei 056029,china,4 institute of cardiovascular research,peking university first hospital,beijing 100034,china Department of cardiovascular Diseases First Affiliated Hospital Shantou University Medical College Shantou China The affiliated hospital Medical School of Hebei University of Engineering Hebei China institute of cardiovascular research Peking University First Hospital Beijing China

Aim:To study whether urotensin II（UII）,a potent vasoconstrictive peptide,isinvolved in the development of cardiac hypertrophy and fibrogenesis of ratsinduced by isoproterenol（ISO）.Methods:Thirty male Wistar rats were randomlydivided into 3 *** 1 was the healthy control group,group 2 was the ISOgroup,and group 3 was the ISO+UII *** groups 2 and 3,ISO(5 mg-kg-1.d-1)wasgiven（sc）once daily for 7 *** 3 was also given UII in the first day [3 nmol/kg（5μg/kg）,iv],followed by sc（1.5 μg/kg）twice *** 1 received 0.9% *** receptor（UT）mRNA expression was determined by *** contents ofUII and angiotensin II（Ang II）were determined by *** vitro,the effects of UII on DNA/collagen synthesis of cardiac fibroblasts were deter-mined by [3H]thymidine/[3H]proline ***:The ratio of heartweight/body weight,plasma lactate dehydrogenase activity,myocardialmalondialdehyde and hydroxyproline concentration increased significantly in theISO group,as well as UT mRNA expression,plasma and cardiac UII and ventricu-lar Ang II,compared with the control group（P3H]thymidine/[3H]proline incorporation into cardiac fibroblasts in a dose-dependent manner（P<0.01）.Conclusion:These results suggest that UII was involved in the development ofcardiac fibrosis and hypertrophy by synergistic effects with ISO.

关键词： cardiac remodeling cardiac fibrosis GPR14 receptor urotensin II collagen

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Analysis of Micro Thermal Environment for Residential District Planning

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湖南大学学报（自然科学版） 2009年第S1期36卷 51-54页

作者： ZHANG Wei-jie1,QI Jing2,JIN Wen2(1.institute of Urban Construction,hebei university of engineering,Handan,hebei 056038,china 2.school of Architecture,hebei university of engineering,Handan,hebei 056038,china) institute of Urban Construction Hebei University of Engineering Handan 056038 China school of Architecture Hebei University of Engineering Handan 056038 China

This paper focused on residential district planning *** GIS and SketchUp software,a common digital relief map model of a residential district was created,and the air distribution(both velocity and temperature)within this residential district was established by CFD ***,the velocity and temperature of the air at any location within the residential district,as well as the worse flow area and overheat area can be clearly *** index of micro thermal environment for the air distribution evaluation was *** on a certain residential district planning model,from the air parameters of the micro environment at any location within the residential district,such as air velocity and air temperature,the index of micro thermal environment was obtained by certain principles and data processing *** this index,the residential district planning scheme was evaluated corresponding to the thermal characteristics of the residential *** this index is not good enough or unsatisfied,the residential district planning idea and process can be changed or improved accordingly in order to get a better thermal characteristics of the residential district judged by the established evaluation index system.

关键词： residential district planning micro thermal environment micro thermal environmental index CFD SketchUp

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Role of inositol 1,4,5-trisphosphate receptors in α1-adrenergic receptorinduced cardiomyocyte hypertrophy

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Acta Pharmacologica Sinica 2006年第7期27卷 895-900页

作者： Da-li LUO~(2,3,5) Jian GAO~2 Xiao-mei LAN~3 Gang WANG~4 Sheng WEI~4 Rui-ping XIAO~4 Qi-de HAN~32 Department of Pharmacology,school of Chemical Biology & Pharmaceutical Sciences,Capital university of medical Sciences,beijing 100069,china 3 institute of cardiovascular Science at Health Science Center,peking university beijing 100083,china 4 institute of Molecular Medicine and College of Life Science,peking university,beijing 100871,china Department of Pharmacology School of Chemical Biology & Pharmaceutical Sciences Capital University of Medical Sciences Beijing China institute of cardiovascular Science at Health Science Center Peking University Beijing China institute of Molecular Medicine and College of Life Science Peking University Beijing China

Aim:Intracellular Ca2+plays pivotal roles in diverse cellular functions,includinggene transcription that underlies cardiac remodeling during stress ***,the role of inositol 1,4,5-trisphosphate receptors（IP3Rs）in the media-tion of cardiac intracellular Ca2+and hypertrophic growth remains *** with neonatal rat ventricular myocytes suggests that activation of IP3Rsmay be linked to α1adrenergic receptor（α1AR）increased stereotyped Ca2+sparkoccurrence and global Ca2+***,we hypothesized that Ca2+releasethrough IP3Rs was necessary for α1AR-stimulated cardiac ***:We used myoinositol 1,4,5-trisphosphate hexakis（butyryloxymethyl）ester（IP3BM）,a membrane-permeant ester of IP3,to activate IP3Rs directly,and Fluo 4/AM tomeasure intracellular Ca2+***:IP3BM(10μmol·L-1)mimicked theeffects of phenylephrine,a selective agonist of α1AR,in increments in local Ca2+spark release（especially in the perinuclear area）and global Ca2+*** importantly,IP3R inhibitors,2-aminoethoxydiphenyl borateand Xestospongin C,abolished the IP3BM-induced Ca2+responses,and signifi-cantly suppressed α1AR-induced cardiomyocyte hypertrophy assayed by cellsize,[3H]leucine incorporation and atrial natriuretic factor gene expression,dur-ing sustained（48 h）phenylephrine ***:These results,therefore,provide cellular mechanisms that link IP3R signaling to α1AR-stimulatedgene expression and cardiomyocyte hypertrophy.

关键词： inositol 1,4,5-trisphosphate receptors Ca2+sparks α1 adrenergic stimulation cardiac hypertrophy

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AICAR inhibits proliferation and induced S-phase arrest,and promotes apoptosis in CaSki cells

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Acta Pharmacologica Sinica 2007年第12期28卷 1984-1990页

作者： Tong-ju GUAN~2,Feng-jin QIN~3,Jian-hai DU~4,Li Geng~3,You-yi ZHANG~(4,5),Min LI~(2,5)~2 institute of Pathology,The school of Basic Medicine,Lanzhou university,Lanzhou 730000,china ~3 Department of Gynaecology andObstetrics,peking university Third hospital,beijing 100083,china ~4 institute of Vascular Medicine,peking university Third hospital andKey Laboratory of Molecular cardiovascular Sciences Ministry of Education,beijing 100083,china institute of Pathology The School of Basic Medicine Lanzhou University Lanzhou China Department of Gynaecology and Obstetrics Peking University Third Hospital Beijing China institute of Vascular Medicine Peking University Third Hospital and Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing China

Aim:The aim of the present study was to determine the effect of 5-aminoimidazole-4-carboxamide-ribonucleoside （AICAR） on proliferation,cell cycle,and apoptosisin the human epithelial cervical cancer cell line CaSki ***:Cell countand 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay were usedto determine cell proliferation and *** 33258 staining was con-ducted to distinguish the apoptotic *** cycle and Annexin-V/propidiumiodide staining were analyzed by fluorescence-activated cell sorting （FACS）.AWestern blot assay was used to evaluate the expression of AKT （also known asprotein kinase B）,mammalian target of rapamycin （mTOR）,p53,and extracellularsignal-regulated kinase （ERK）.Results:AICAR （500 μmol/L） significantly inhibi-ted the proliferation of CaSki cells treated for 24,48,and 72 h as determined by *** cells at the G1and G2phases were dramatically decreased while cells atthe S phase were increased in response to AICAR treatment for 24,48,and 72 *** MTT assay showed less viable cells and Hoechst fluorescent staining showedmore apoptotic cells upon AICAR *** results of the Annexin-Vstaining demonstrated a time-dependent increase of apoptosis in cells treatedwith AICAR for 24,36,and 48 ***,AICAR activated caspase-3 in atime-dependent *** was also found that AICAR inhibited the phosphory-lation of AKT and mTOR,which are important kinases regulating cell growth *** stimulation obviously increased the expression of the tumorsuppressor p53 and the phosphorylation of ***:AICAR inhibitedproliferation and induced S phase arrest and promoted apoptosis in CaSki cells,which might be mediated by the downregulation of the AKT/mTOR pathway andthe upregulation of the p53/ERK pathway.

关键词： AICAR proliferation apoptosis CaSki cells

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Morita contexts on the Hopf algebroid

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Science china Mathematics 2005年第7期48卷 958-971页

作者： YIN Yanmin school of Mathematical Sciences, peking university, beijing 100871, china Department of Mathematics, Shandong institute of Architecture and engineering, Jinan 250101, china school of Mathematical Sciences Peking University Beijing 100871 China Department of Mathematics Shandong Institute of Architecture and Engineering Jinan 250101 China

We generalize the Morita theory to the Hopf algebroid and give the characterization of the surjectivity of one of the Morita context maps.

关键词： comodule algebra, generalized smash product, Morita context.

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Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo

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Acta Pharmacologica Sinica 2006年第12期27卷 1608-1615页

作者： Lin LIN~2 Xin FU~2 Xin ZHANG~2 Lian-xu CHEN~2 Ji-ying ZHANG~2 Chang-long YU~(2,4)Kang-tao MA~3 Chun-yan ZHOU~(3,4)2 institute of Sports Medicine,peking university Third hospital,beijing 100083,china 3 Department of Biochemistry and Molecular Biology,peking university school of Basic medical Science,beijing 100083,china institute of Sports Medicine Peking University Third Hospital Beijing China Department of Biochemistry and Molecular Biology Peking University School of Basic Medical Science Beijing China

Aim:Bone morphogenetic protein 4(BMP4)is one of the main local contributingfactors in callus formation in the early phase of fracture ***-derivedstromal cells(ADSC)are multipotent *** present study was conducted toinvestigate the osteogenic potential of ADSC when exposed to adenovirus con-taining BMP4 cDNA(Ad-BMP4).Methods:ADSC were harvested from Sprague-Dawley *** exposure to Ad-BMP4,ADSC were assessed by alkaline phos-phatase activity(ALP)assay,RT-PCR and yon Kossa ***4 expressionwas assessed by RT-PCR,immunofluorescence and Western blot analysis,ADSCtransduced with Ad-BMP4 were directly injected into the hind limb muscles ofathyrnic *** Ad-EGFP(enhanced green fluorescence protein)served *** animals were examined by X-ray film and histological ***:The expression of BMP4 was confirmed at both mRNA and protein *** expression of the osteoblastic gene,ALP activity and yon Kossa stainingconfirmed that ADSC transduced with Ad-BMP4 underwent rapid and markedosteoblast differentiation,whereas ADSC transduced with Ad-EGFP and cells leftalone displayed no osteogenic differentiation.X-ray and histological examinationconfirmed new bone formation in athymic mice transplanted with ADSC trans-duced with ***:Our data demonstrated successful osteo-genic differentiation of ADSC transduced with Ad-BMP4 in vitro and in *** may be an ideal source of mesenchyme lineage stem cells for gene therapyand tissue engineering.

关键词： adipose-derived stromal cells BMP4 gene therapy bone formation

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The precipitation and effect of nano nitrides in HSLC steel

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Science china(Technological Sciences) 2008年第7期51卷 989-998页

作者： FU Jie1, LIU YangChun1,2 & WU HuaJie1,3 1 school of Metallurgical and Ecological engineering, university of Science and Technology beijing, beijing 100083, china 2 Shougang Technology research institute, Shougang (Group) Corporation, beijing 100041, china 3 Metallurgical engineering research institute, university of Science and Technology beijing, beijing 100083, china school of Metallurgical and Ecological engineering University of Science and Technology Beijing Beijing China Shougang Technology research institute Shougang (Group) Corporation Beijing China Metallurgical engineering research institute University of Science and Technology Beijing Beijing China

The existing forms of N and Al in HSLC (high strength low carbon) steel produced by TSCR (thin slab casting and rolling process), the precipitation thermodynamics and kinetics of AlN, and its effects on structure and mechanical property are studied. The experimental results show that only a small quantity of nitrogen is com- bined into AlN in HSLC steel produced by TSCR and most of the nitrogen in steel is still free nitrogen. Aluminum-nitride is mainly precipitated during the period of slow air cooling after coiling, but not during rolling and water cooling. The acid-soluble aluminum has no obvious effect on the grain size and mechanical property of HSLC steel produced by TSCR whose acid-soluble aluminum content is 0.005%―0.043%. The precipitation of AlN is not the main cause of grain refinement of HSLC steel produced by TSCR, nor is AlN the dominating precipitate that has precipitation strengthening effect. The nano nitrides are not pure AlN, but have complex compositions.

关键词： thin slab casting and rolling high strength low carbon steel AlN nano nitride precipitation strengthening

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Identification and removal of block ambiguities in sea surface wind field with scatterometers

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Science china Earth Sciences 2009年第2期52卷 242-249页

作者： CHEN KeHai1,2, XIE XueTong3 & FANG Yu4 1 South china Sea institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, china 2 Graduate university of Chinese Academy of Sciences, beijing 100049, china 3 school of Electronic Science and engineering, National university of Defense Technology, Changsha 410073, china 4 The institute of Remote Sensing and GIS, peking university, beijing 100871, china South china Sea institute of Oceanology Chinese Academy of Sciences Guangzhou China Graduate university of Chinese Academy of Sciences Beijing China school of Electronic Science and engineering National University of Defense Technology Changsha China The institute of Remote Sensing and GIS Peking University Beijing China

The solution of the current wind retrieval algorithm for scatterometers has several wind vector ambi-guities, due to the bi-harmonic relationship between normalized backscattering cross section and the relative wind direction and the existence of the measurement error. In order to remove the ambiguities for a unique wind field, a circular median filter approach (CMF) is usually adopted. But under the con-dition for clustering distribution of the false ambiguities in some local areas, the CMF fails and thus engenders block ambiguities, which degrade the precision of the retrieved wind field. For such a situation, a technique of identification and removal of the block ambiguities is presented to further optimize the retrieved wind field after CMF. It is demonstrated in experiment that this technique can identify and remove most of the block ambiguities.

关键词： scatterometer block ambiguity identification and removal

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PDMS prism-glass optical coupling for surface plasmon resonance sensors based on MEMS technology

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Science china(Information Sciences) 2010年第10期53卷 2144-2158页

作者： GENG ZhaoXin 1,2 , LI Qing 3 , WANG Wei 1 & LI ZhiHong 1 1 institute of Microelectronics, peking university, beijing 100871, china 2 school of Information engineering, Minzu university of china, beijing 100081, china 3 school of Sciences, Changchun university of Science and Technology, Changchun 130000, china institute of Microelectronics Peking University Beijing China school of Information engineering Minzu University of China Beijing China school of Sciences Changchun University of Science and Technology Changchun China

A miniaturized surface plasmon resonance (SPR) chip has been developed for biomedical and chem-ical analysis with low cost and high performance. The techniques of bulk silicon micromachining and polymer replication were used to fabricate the Kretschmann SPR sensor composed of a Polydimethylsiloxane (PDMS) prism, a coupling glass and microchannels. The plasmon properties of thin metal films were investigated theoret-ically based on Fresnel analysis, with optical boundary conditions pertaining to the surface plasmon resonance at the gold/water and gold/air interfaces. The theoretical results show that difference in the refractive index (RI) between the PDMS prism and the coupling glass layer affect the precision of the SPR angle and the SPR curve. Meanwhile, the period of the interference fringe attaching on the SPR curve increases with an increase in wavelength and a decrease in the refractive index of the coupling glass layer. The gold thickness of 50 nm is required while employing a fixed incident wavelength of 650 nm, to achieve optimum SPR excitation conditions and the sensor sensitivity. The characteristics of this SPR sensor were evaluated in the angular interrogation mode of employing the incident wavelength of 650 nm in air and water media, respectively. The obtained SPR angles were approximately consistent with the theoretical ones.

关键词： surface plasmon resolance polymer microfluidic PDMS

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Design of an MRI quadrature-data acquisition card

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Progress in Natural Science:Materials International 2006年第3期16卷 255-259页

作者： JIANG Zhongde, ZU Donglin and GU Xiaofang (institute of Heavy Ion Physics, beijing Key Laboratory of medical Physics & engineering, school of Physics, peking university,beijing 100871, china) institute of Heavy Ion Physics Beijing Key Laboratory of Medical Physics and Engineering School of Physics Peking University Beijing 100871 China

A design of a quadrature-data acquisition card based on peripheral component interconnect (PCI) bus for mini-type magnetic resonance imaging (MRI) system is reported. It uses two high speed analog-to-digital converters (ADCs) to sample the MRI signals and two static random access memories (SRAMs) to store the data which will be read to the computer by PCI bus after sampling. All the logic control signals on the card are generated by the field programmable gate array (FPGA). The software Foundation3.1 is used to design the FPGA and achieve useful result after simulating and implementing. The card has some merits that normal commercial cards do not have. For example, the sampling parameters can be varied according to different pulse sequences.

关键词： MRI data acquisition FPGA PCI bus.

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