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Dynamics of morphological changes for mitochondrial fission and fusion

Science china(Physics,Mechanics & Astronomy) 2010年第4期53卷 680-689页

作者： WANG ShiQi 1,2,4 , FU ChangLiang 1,2 , ZHANG Yan 1,2 , CHEN Quan 3 & LONG Mian 1,2 1 key laboratory of Microgravity (National Microgravity laboratory), beijing 100190, china 2 Center for Biomechanics and Bioengineering, institute of Mechanics, chinese academy of sciences, beijing 100190, china 3 state key laboratory of biomembrane and membrane biotechnology, institute of zoology, chinese academy of sciences, beijing 100101, china 4 institute of Vertebrate Paleontology and Paleoanthropology, chinese academy of sciences, beijing 100044, china key laboratory of Microgravity (National Microgravity laboratory) Beijing China Center for Biomechanics and Bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing China institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Beijing China state key laboratory of biomembrane and membrane biotechnology Institute of Zoology Chinese Academy of Sciences Beijing China

Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.

关键词： mitochondria morphology fission fusion dynamics modeling

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Mitochondrial membrane fusion and ATP synthesis both compete for interfacial △pH of PMF,but not △Ψ:An Inter-play between membrane fusion and energy coupling of mitochondria

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生物物理学报 2009年第S1期25卷 217-218页

作者： Shusen Liu state key laboratory of biomembrane and membrane biotechnology, Instutute of zoology, chinese academy of sciences, beijing, 100101, china

Although some fusogenic proteins have been established to tether two opposite outer or inner mitochondrial membranes into contact, a prerequisite for whole mitochondrial fusion events,

关键词： Mitochondrial membrane fusion Protein-tethering Lipid-bilayer-mixing and fusion Interfacial △pH of PMF.Inter-play between membrane fusion and ATP synthesis.

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Molecular basis of cryptorchidism-induced infertility

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Science china(Life sciences) 2010年第11期53卷 1274-1283页

作者： LIU YiXun & LI XiXia state key laboratory of Reproductive Biology, institute of zoology, chinese academy of sciences, beijing 100101, china state key laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing China

Artificial cryptorchidism or local testicular heat treatment can induce reversible oligospermia or azoospermia in monkeys and rats via germ cell apoptosis. Local warming of monkey testes in water at 43°C for 2 consecutive days (30 min per day) decreased the number of sperm in the semen by up to 80% on d 28, and the effect was completely reversed on d 144. Germ cells rely heavily on Sertoli cells for structural and nutritional support. Specialized junctions that play a pivotal role in spermatogenesis occur at sites of Sertoli-Sertoli and Sertoli-germ cell contact in the seminiferous epithelium. We demonstrated that expression of tight junction (TJ)-associated molecules, such as occludin and zonula occludens-1 (ZO-1), were greatly reduced 24–48 h after heat treatment, while the permeability of the blood-testis barrier (BTB) was simultaneously increased, but recovered 10 d later. These results indicate a reversible disruption of the BTB associated with transient inductions of transforming growth factor (TGF) β2 and β3 expression, p38 mitogen-activated protein kinase and extracellular signal-regulated kinase activation, and concomitant loss of occludin and ZO-1. This suggests that expression of TJ-associated molecules and the BTB was reversibly perturbed by mild testicular hyperthermia, and that the heat-induced induction of TGF-β might be involved in downregulating TJ-associated proteins, leading to cell junction reduction. This review discusses the changes in total gene expression patterns after experimental cryptorchidism in adult mouse testes, and the cloning of several novel, physiologically significant spermatogenesis-specific genes.

关键词： cryptorchidism spermatogenesis apoptosis heat stress Sertoli cells blood-testis barrier tight junction

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Role of ubiquitination in meiotic recombination repair

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Science china(Life sciences) 2010年第4期53卷 447-454页

作者： CUI Li & LI Wei state key laboratory of Reproductive Biology, institute of zoology, chinese academy of sciences, beijing 100101, china state key laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing China

Programmed and unprogrammed double-strand breaks (DSBs) often arise from such physiological requirements as meiotic recombination, and exogenous insults, such as ionizing radiation (IR). Due to deleterious impacts on genome stability, DSBs must be appropriately processed and repaired in a regulatory manner. Recent investigations have indicated that ubiquitination is a critical factor in DNA damage response and meiotic recombination repair. This review summarizes the effects of proteins and complexes associated with ubiquitination with regard to homologous recombination (HR)-dependent DSB repair.

关键词： ubiquitin meiosis homologous recombination DNA double-strand breaks

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Negative regulation of TGF-β signaling in development

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Cell Research 2004年第6期14卷 441-449页

作者： Ye Guang CHEN, An Ming MENG The state key laboratory of biomembrane and membrane biotechnology, Department of Biological sciences and biotechnology, Tsinghua University, beijing 100084, china Department of Biological sciences and biotechnology The State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China

The TGF-β superfamily members have important roles in controlling patterning and tissue formation in both invertebrates and vertebrates. Two types of signal transducers, receptors and Smads, mediate the signaling to regulate expression of their target genes. Despite of the relatively simple signal transduction pathway, many modulators have been found to contribute to a tight regulation of this pathway in a variety of mechanisms. This article reviews the negative regulation of TGF-β signaling with focus on its roles in vertebrate development.

关键词： TGF-β BMP Nodal Activin antagonist development negative regulation.

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Structure and regulatory function of plant transcription factors

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chinese Science Bulletin 2001年第4期46卷 271-278页

作者： LIU Qiang, ZHANG Guiyou & CHEN ShouyiDepartment of Biological Science and biotechnology, Tsinghua Univorsity, beijing 100084, china Department of Biological Science and biotechnology, state laboratory of biomembrane and membrane biotechnology, Tsinghua University, beijing 100084, china institute of Genetics, chinese academy of sciences, beijing 100101, china Department of Biological Science and biotechnology Tsinghua University Beijing China Department of Biological Science and biotechnology State Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China institute of Genetics Chinese Academy of Sciences Beijing China

The expression of inducible genes in plants is regulated by specific transcription factors at the transcrip-tional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulation domain, a dimerization site and a nuclear localization domain. These functional domains define the characteristic, localization and regulatory role of a transcription factor. Transcription factors recognize and bind to specific cis-acting elements or interact with other proteins, and then activate or repress the transcription of target genes by their functional domains. In recent years, elucidation on the structure and function of transcription factors has become an important subject in plant molecular biology.

关键词： transcription factor DNA-binding domain cis-acting element.

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NF-κB,a hot topic in biochemical and medical studies in china

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Science china(Life sciences) 2010年第12期53卷 1485-1487页

作者： WEI Yan state key laboratory of Brain and Cognitive sciences,institute of Biophysics,chinese academy of sciences,beijing 100101,china state key laboratory of Brain and Cognitive sciences Institute of Biophysics Chinese Academy of Sciences Beijing China

In 1986, nuclear factor (NF)-κB was first discovered in the laboratory of Nobel Prize laureate David Baltimore via its interaction with a sequence (5′-GGGACTTTCC-3′) in the immunoglobulin light-chain enhancer in B

关键词： NF-κB transcription factor signaling pathway upregulation downregulation

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Thymic epithelial progenitor cells and thymus regeneration: an update

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Cell Research 2007年第1期17卷 50-55页

作者： L ianjun Zhang~(1,2) Liguang Sun~(1,3) Yong Zhao~1 ~1Transplantation Biology Research Division,state key laboratory of biomembrane and membrane biotechnology,institute of zoology,~2Graduate University of the chinese academy of sciences,chinese academy of sciences,beijing 100080,china,~3School of Public Health,Jilin University,Changchun 130021,china Transplantation Biology Research Division State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Sciences Beijing China Graduate University of the chinese academy of sciences Chinese Academy of Sciences Beijing China School of Public Health Jilin University Changchun China

The thymus provides the essential microenvironment for T-cell development and *** epithelial cells(TECs),which are composed ofthymic cortical epithelial cells (cTECs) and thymic medullary epithelial cells (mTECs),have been well documented to be critical for these tightly regulated *** has long been controversial whether thecommon progenitor cells of TECs could give rise to both cTECs and *** progress has been made to charac-terize the common TEC progenitor cells in recent *** herein discuss the sole origin paradigm with regard to TECdifferentiation as well as these progenitor cells in thymus regeneration.

关键词： thymic epithelial progenitor cells thymus organogenesis thymus regeneration

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GM-CSF-induced Ca_(2+) -activated non-voltage-dependent inwardly rectifying K_+ channel in murine peritoneal exudate macrophages

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chinese Science Bulletin 1997年第11期42卷 947-952页

作者： ZHANG Xiaofeng, FENG Meifu, WU Caihong and ZHOU Pei’aistate key laboratory of biomembrane and membrane biotechnology, chinese academy of sciences, beijing 100080, china 1 . In-stitute of zoology, chinese academy of sciences, beijing 100080, china 2 College of Life sciences, Peking University, beijing 100871, china state key laboratory of biomembrane and membrane biotechnology Chinese Academy of Sciences Beijing China institute of zoology Chinese Academy of Sciences Beijing China College of Life sciences Peking University Beijing China

GRANULOCYTE-MACROPHAGE colony-stimulating factor(GM-CSF),a member in the family ofglycoprotein cytokines,can not only act to stimulate proliferation of immature progenitors,butalso enhance differentiated functions of mature effector *** has two kinds of biologicaleffects on mature effector cells,*** effect and indirect *** latter is also

关键词： K+ channel patch clamp GM-CSF macrophage.

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Induction of chromosomal inversion by integration of T-DNA in the rice genome

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Journal of Genetics and Genomics 2010年第3期37卷 189-196页

作者： Chuanfeng Zhu,Jiahe Wu,Chaozu He state key laboratory of Plant Genomics,institute of Microbiology,chinese academy of sciences,beijing 100101,china state key laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China

Transfer DNA （T-DNA） of Agrobacterium tumefaciens integration in the plant genome may lead to rearrangements of host plant chromosomal fragments, including inversions. However, there is very little information concerning the inversion. The present study re- ports a transgenic rice line selected from a T-DNA tagged population, which displays a semi-dwarf phenotype. Molecular analysis of this mutant indicated an insertion of two tandem copies of T-DNA into a locus on the rice genome in a head to tail mode. This insertion of T-DNA resulted in the inversion of a 4.9 Mb chromosomal segment. Results of sequence analysis suggest that the chromosomal inversion resulted from the insertion of T-DNA with the help of sequence microhomology between insertion region of T-DNA and target sequence of the host plant.

关键词： Agrobacterium tumefaciens-mediated transformation (ATMT) T-DNA chromosomal inversion

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