Induction of chromosomal inversion by integration of T-DNA in the rice genome

作     者：Chuanfeng Zhu,Jiahe Wu,Chaozu He State Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China 

作者机构：[a] State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2010年第37卷第3期

页      面：189-196页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National High Technology Development Program of China (No.2008AA10Z104) China Postdoctoral Science Foundation (No.20060600519) 

主　　题：Agrobacterium tumefaciens-mediated transformation (ATMT) T-DNA chromosomal inversion 

摘      要：Transfer DNA （T-DNA） of Agrobacterium tumefaciens integration in the plant genome may lead to rearrangements of host plant chromosomal fragments, including inversions. However, there is very little information concerning the inversion. The present study re- ports a transgenic rice line selected from a T-DNA tagged population, which displays a semi-dwarf phenotype. Molecular analysis of this mutant indicated an insertion of two tandem copies of T-DNA into a locus on the rice genome in a head to tail mode. This insertion of T-DNA resulted in the inversion of a 4.9 Mb chromosomal segment. Results of sequence analysis suggest that the chromosomal inversion resulted from the insertion of T-DNA with the help of sequence microhomology between insertion region of T-DNA and target sequence of the host plant.