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A proteomic approach to analyzing responses of Arabidopsis thaliana root apex cells to different gravitational conditions using an agravitropic mutant,pin2 and its wild type

A proteomic approach to analyzing responses of Arabidopsis t...

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“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Chao Tan,Hui Qiong Zheng institute of plant physiology and ecology.shanghai institutes for biological sciences.chinese academy of sciences.300 Fenglin Road, shanghai 2.0032.china

We have comparatively analyzed the proteomes of Arabidopsis thaliana wild type(***) and pin2 roots subjected to different gravitational conditions,including horizontal(H) and vertical(V) clinorotation, hypergravity(G) and the stationary control(S) by two-dimensional(2-D) gel electrophoresis and microbe high performance liquid chromatography-ion trap-mass spectrometry(LC-IT-MS) to obtain the tandem mass(MS/MS) *** analysis of silver-stained 2-D gels revealed that 28 protein spots showed significant expression changes in altered gravity(H or G) treated samples compared to the control(V and S samples).Most of them,including eight H responsive proteins and fifteen G responsive proteins, exhibited similar expression pattern in wild type and pin2 *** contrast,a substantial fraction displayed different patterns of response between wild type and pin2 roots were also detected,including eleven protein spots in the H samples and two protein spots in the G samples exhibited altered expression exclusively in wild type but not in pin2 roots,while expression of four proteins in the H sample and two in the G sample had elevated in pin2 root tip and apparently unchanged in wild type ***2 is important for the basipetal transport of auxin in roots,which is important to signal the root elongation zones of gravity signals perceived in the root ***,observation of different protein behaviors between wild type and pin2 mutant root tips in this study has implications as to where such changes might be likely to occur in the *** idea is strengthen by observation of differential increase of ANN2-GFP signals in the columella cells of WT and pin2 plants subject to the H or G *** data present in this study have potentially important implications for our understanding of gravity sensing and signal transduction in Arabidopsis root tips.

关键词： Proteomic PIN2 Altered gravity Arabidopsis thaliana

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浑球红细菌 (Rhodobacter sphaeroides)中氢化酶正调节基因hupR的克隆及功能分析(英文)

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生物化学与生物物理学报 2001年第6期33卷 607-614页

作者：徐冬青吴永强中国科学院上海生命科学研究院植物生理生态研究所中国科学院上海生命科学研究院植物生理生态研究所上海2.0032

从光合细菌Rhodobactersphaeroides基因文库中分离出含有氢化酶基因簇 (hup)的粘粒cosmid 1后 ,亚克隆了R .sphaeroides的氢化酶调节基因hupR ,测定了hupR的核苷酸序列 ,并完成了氢化酶基因簇的部分物理图谱。实验结果表明 ,hupR基因全... 详细信息

从光合细菌Rhodobactersphaeroides基因文库中分离出含有氢化酶基因簇 (hup)的粘粒cosmid 1后 ,亚克隆了R .sphaeroides的氢化酶调节基因hupR ,测定了hupR的核苷酸序列 ,并完成了氢化酶基因簇的部分物理图谱。实验结果表明 ,hupR基因全长 1476bp ,编码的HupR蛋白分子量约为 5 4.0 31kD (EMBL接受号 :AJ2 43734 )。与R .capsulatus中HupR相比 ,同源性高达 73%。同源性比较结果表明 ,它属于双组分调节系统中受体蛋白。hupR基因在E .coli中进行了体外表达 ,纯化后测定得到的HupR蛋白的分子量大小与hupR基因推测的分子量大小一致。通过双交换 ,将卡那霉素抗性基因插入hupR基因 ,获得丧失氢化酶活性的hupR-的突变株 ,KR5和KR7。hupS∷lacZ融合基因在野生型中的转录表达量是在该突变株中的 7～ 9倍。将hupR基因置于弱启动子 pfru下游 ,构建了质粒pNRC3,并将其导入hupR-的突变株 ,可使突变株重新获得氢化酶活性。以上结果说明 ,HupR蛋白对氢化酶的转录表达起着正调节作用。在HupR蛋白的磷酸化区域进行定点和缺失突变 ,不影响HupR激活氢化酶基因的表达 ,推测HupR蛋白是在非磷酸化的状态下起调节作用的。

关键词： Rhodobacter sphaeroides hup基因簇 hupR基因转录氢化酶

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Two Types of Cellulose Synthase Complexes are Involved in the Cell Wall for Mation of the Differentiating Xylem in Populu

Two Types of Cellulose Synthase Complexes are Involved in th...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Dongliang Song, Junhui Shen, Laigeng Li* institute of plant physiology and ecology. The shanghai institutes for biological sciences. chinese academy of Science, shanghai, 2.0032. china

＜正＞It is generally believed that the synthesis of cellulose in higher plants is mediated by cellulose synthase complexes （CSCs） localized on the plasma membrane. There is one type of CSC reported in the cellulose

关键词： Cellulose synthesis Cell walls Cellulose synthase complex.

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Coordinated Replication of Two Internal Origins on Streptomyces Linear Plasmid pFRL1

Coordinated Replication of Two Internal Origins on Streptomy...

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2010年第四届全国微生物遗传学学术研讨会

作者： Ran Zhang and Zhongjun Qin~* Key Laboratory of Synthetic Biology,shanghai institute of plant physiology and ecology. shanghai institutes for biological sciences.the chinese academy of sciences.shanghai,china

Previous reports showed that Streptomyces linear plasmids usually contain one internal replication ***,we identified two new replication loci on pFRL1,one (rep1A/ncs1) next to a telomere and another(rep2A/ncs2)～10 kb from *** rep1A/ncs1 locus was able to direct replication independently in both linear and circular modes,whereas rep2A/ncs2 required an additional locus,rlrA/rorA,in order

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Development of a gene cloning system in fast-growing and moderately-thermophilic Streptomyces and heterologous expression of Streptomyces antibiotics biosynthetic gene clusters

Development of a gene cloning system in fast-growing and mod...

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2010年第四届全国微生物遗传学学术研讨会

作者： Weihua Chen and Zhongjun Qin~* Key Laboratory of Synthetic Biology,shanghai institute of plant physiology and ecology. shanghai institutes for biological sciences.the chinese academy of sciences.shanghai,china

Slow-growing,mesophilic Streptomyces species are a major natural source of antibiotics,whereas fast-growing,thermophilic Streptomyces strains have hardly been *** report here the isolation of 39 fast-growing,moderately thermophilic Streptomyces strains,detection of one linear and three circular plasmids in them,and

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A“Minus and Plus”Method for Cloning Large Genomic Segments in the Linear Plasmid SCP1 of Streptomyces coelicolor A3(2)

A“Minus and Plus”Method for Cloning Large Genomic Segments...

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2010年第四届全国微生物遗传学学术研讨会

The model organism Streptomyces coelicolor A3(2) harbors a 356-kb linear plasmid,*** report here development of a"minus and sequential plus"method for deleting large segment from one telomere of SCP1 followed by seque...

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Inactivation of AtSIG6 Suppresses the Leaf Variegation of Thf1 in Arabidopsis

Inactivation of AtSIG6 Suppresses the Leaf Variegation of Th...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Fenhong Hu, Yungang Shen, Jirong Huang* 1institute of plant physiology and ecology. shanghai institutes for biological sciences. chinese academy sciences. shanghai 2.0032. china.

＜正＞Leaf variegation is defined as a leaf displaying green sectors and non-green sectors. It can be caused by mutations in nuclear, chloroplast and mitochondrial genes. The Arabidopsis thylakoid formation 1 （Thf1）... 详细信息

关键词： THF1 Leaf variegation AtSIG6 Suppressor

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Cloning of the Gene Responsible for the Yellow Cotyledon and Reduced Accumulation of Anthocyanins in Arabidopsis

Cloning of the Gene Responsible for the Yellow Cotyledon and...

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纪念殷宏章先生百年诞辰暨全国光合作用学术研讨会

作者： Yi Sun, Jirong Huang shanghai institute of plant physiology and ecology. shanghai institutes for biological sciences. chinese academy of sciences. 300 Feng Lin Road, shanghai 2.0032

＜正＞Flavonoids play important roles in recruitment of pollinators and seed dispersers, protecting plants against various stresses, being signals between plants and microbes and regulating auxin transport. They also ... 详细信息

关键词： Gene Clone Yellow cotyledon Reduced Accumulation Anthocyanins Arabidopsis

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Cytoplasmic auxin signaling

Cytoplasmic auxin signaling

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从植物科学到农业发展——2012全国植物生物学大会

作者： Zhenbiao Yang Department of Botany and plant sciences.and Center for plant Cell Biology,University of California at Riverside,CA 92521,USA shanghai institute of plant physiology and ecology.shanghai institutes of biological sciences.chinese academy of sciences.shanghai,china.

Auxin regulates the formation of various developmental and morphogenetic patterns in plants,but the molecular and cellular mechanisms for the auxin actions are not well *** investigate these mechanisms using Arabidopsis leaf epidermal pavement cells(PCs) as a model system,which form the jigsaw puzzle cell pattern with interdigitated lobes and *** have shown that pavement cell interdigitation is controlled by localized ROP GTPase signaling,which impinges on the organization of cortical actin microfilaments and microtubules to generate interdigitating lobes and *** recent studies indicate that auxin is a signal that activates the generation of multi-polarity to form lobes in pavement cells and coordinates lobe formation with indentation formation by activating ROP GTPase signaling through an Auxin-Binding Protein 1(ABP1) dependent auxin perception *** cytoplasmic auxin signaling system,which is distinct from the well-established TIR1-dependent nuclear auxin signaling system that regulates gene transcription,modulates local coordination of lobe and indentation formation within a PC and between adjacent PCs through its modulation of PIN protein *** ROP-based cytoplasmic auxin signaling also regulates the subcellular distribution of PINs in root cells,supporting a widespread role for this new auxin signaling system in plants 4. Recent findings of additional components in this new auxin signaling system will be discussed.

关键词： AUXIN SIGNALING ABP1 PIN1 PIN2

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Defect in Degradation of PSII Complex Causes a Stay-green Phenotype during Dark-Induced Senescence in Arabidopsis thf1 Mutant

Defect in Degradation of PSII Complex Causes a Stay-green Ph...

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纪念殷宏章先生百年诞辰暨全国光合作用学术研讨会

作者： Fenhong Hu, Jirong Huang, Yungang Shen shanghai institute of plant physiology and ecology. shanghai institutes for biological sciences. chinese academy of sciences. 300 Feng Lin Road, shanghai 2.0032

＜正＞THF1 （Thylakoid Formation 1） is conserved in all oxygenic organisms. The null mutant thf1 exhibits yellow cotyledons, variegated leaves and slower growth rate. The previous studies have shown that THF1 is invo... 详细信息

关键词： Arabidopsis THF1 (Thylakoid Formation 1) PSII complex Stay-green phenotype

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