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Construction of recombinant plasmid and prokaryotic expression in E. Coli and biological activity analysis of human placenta arresten gene

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Hepatobiliary & Pancreatic Diseases International 2006年第1期5卷 74-79页

作者： Jin-Ping Zheng, Hai-Ying Tang, Xian-Jiu Chen, Bao-Feng Yu, Jun Xie and Tang-Chun Wu department of Toxicology (and department of Biochemistry and Molecular Biology Shanxi Medical university, Taiyuan 030001, china: Institute of Occupational Medicine, Tongji Medical College, Huazhong university of Science and Technology, wuhan 430030, china department of Toxicology Shanxi Medical Universiy Taiyuan 030001 China. zhengzheng200101@***

BACKGROUND: The proliferation and metastasis of cancers depend on angiogenesis. This property provides the feasibility for the treatment of cancer by inhibition of angiogenesis, and many angiogenic inhibitors have been demonstrated to effectively inhibit angiogenesis and consequently the growth of solid cancer. As for the newly identified angiogenesis inhibitor, arresten, some studies have found its high activity on restrainting tumor vessel. This study was to assess the anti-angiogenic activity of arresten. METHODS: The arresten gene was obtained from a healthy puerpera's placenta tissue by the reverse transcriptase-polymerase chain reaction (RT-PCR) method, and molecular cloning to prokaryotic expression plasmid pBV220 by recombination strategy. The prokaryotic expression plasmid pBV220/arr was identified by restriction enzyme digestion and sequenced. The pBV220/arr was transformed into E. coli JM109, DH5α, BL21 and BL21 (DE3) by the CaCl_2 transformation method. The arresten expression level was detected by SDS-PAGE. The expressed product was purlfled, re-naturalized and detected for its biological activity of inhibiting the angiogenesis of chorioallantoic membrane (CAM). RESULTS: The arresten gene was cloned and pBV220/arr was constructed. The arresten expression level of protein was highly increased after pBV220/arr was transformed into E. coli BL21 (DE3). SDS-PAGE showed that the expressed arresten proteins were mainly inclusion bodies and had a molecular weight of 26 kDa. The expressed arresten protein showed evident biological activities. CONCLUSIONS: The successful construction of recombinant plasmid pBV220/arr and the effective expression in E. coil have laid a foundation for further study of its anti-angiogenic function and may pave the way for future antitumor application.

关键词： arresten prokaryotic expression vector gene cloning and expression biological activity

EXPERIMENTAL STUDY OF 3-PHOTON ANNIHILATION OF POSITRONS AND ELECTRONS

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Science china Mathematics 1989年第7期32卷 836-846页

作者：唐孝威 Institute of High Energy physics Academia Sinica Beijing department of Modern physics University of Science and Technology of China Hefei

The results of a series of experiments on 3-photon annihilation of positrons and electrons, including the energy-angular correlation, angular distribution, energy spectrum and linear polarization of the annihilation photons, the method of measuring the intensities of 3-photon annihilation, and the upper limit of axion production in the triplet positronium decay, are reported.

关键词： 3-photon annihilation positronium energy spectrum of photons linear polarization of photons axion

Technology for Hybrid Pepper Seed Production in china

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Hunan Agricultural Science ＆ Technology Newsletter 2002年第2期3卷 17-19页

作者： Zou Xuexiao Huazhong university of Science and Technology, wuhan 430074, china Huazhong university of Science and Technology Wuhan 430074 China

History of hybrid pepper seed production, the status of annually balanced production, and innovative techniques for the large-scale seed production in china are reviewed. Helped by the technological breakthroughs in t... 详细信息

关键词： pepper (Capsicum annuum L.) hybrid seed scale production.

Saturation Effects in Mode-competition Effects of a Two-mode Laser with Multiplicative Noise

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Chinese Journal of Lasers 1999年第2期 67-71页

作者： CHEN Limei 1,2 CAO Li 1,3 QIU Junlin 2 ( 1 department of physics, 2 National Laboratory of Laser Technology, Huazhong university of Science and Technology, wuhan 430074, china 3 CCAST (World Laboratory), Beijing 1

１ＩｎｔｒｏｄｕｃｔｉｏｎＴｗｏｍｏｄｅｌａｓｅｒｓ（ＴＭＬ）ｈａｖｅｂｅｎｔｈｅｓｕｂｊｅｃｔｏｆｅｘｔｅｎｓｉｖｅｉｎｔｅｒｅｓｔｉｎｒｅｃｅｎｔｙｅａｒｓ．Ｍｏｄｅｃｏｍｐｏｔｉｔｉｏｎｅｆｅｃｔｉｓｏｎｅｏ... 详细信息

１ＩｎｔｒｏｄｕｃｔｉｏｎＴｗｏｍｏｄｅｌａｓｅｒｓ（ＴＭＬ）ｈａｖｅｂｅｎｔｈｅｓｕｂｊｅｃｔｏｆｅｘｔｅｎｓｉｖｅｉｎｔｅｒｅｓｔｉｎｒｅｃｅｎｔｙｅａｒｓ．Ｍｏｄｅｃｏｍｐｏｔｉｔｉｏｎｅｆｅｃｔｉｓｏｎｅｏｆｔｈｅｉｍｐｏｒｔａｎｔｐｒｏ...

关键词： saturation effect mode competition effect multiplicative noise

Effect and molecular mechanism of indole-3-carbinol on liver fibrosis

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Effect and molecular mechanism of indole-3-carbinol on liver...

Non-isothermal decomposition kinetics and lifetime of Tb2(0-MBA)6(PHEN)2

中国药理学会第十次全国学术会议

作者： PING Jie,GAO Ai-mei,WANG Hui~* (department of Pharmacology,Basic Medical School of wuhan university,wuhan 430071,china)

Aim:Indole-3-carbinol(I3C) is a compound found at higher concentration in cruciferous *** aim of the study is to explore the possible protective effect and molecular mechanism of I3C on liver *** Two animal models of ...

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Rare Metals 2003年第3期22卷 155-159页

作者： ZHANGJianjun BAIJihai WANGRuifen WANGShuping LIJibiao ExperimentalCenter NormalUniversityShijiazhuang050016China departmentofComputer HebeiNormalCollegeofScienceandTechnologyChangli066600China departmentofChemistry HebeiNormalUniversityShijiazhuang050091China

The thermal decomposition of Tb_2(O-MBA)_6(PHEN)_2 (O-MBA: o-methylbenzoate;PHEN: 1,10-phenanthroline) and its kinetics were studied under the non-isothermal condition bythermogravimetry-derivative thermogravimetry (TG-DTG) techniques. Kinetic parameters were obtainedfrom analysis of TG-DTG curves by the Achar method and the Madhusudanan-Krishnan-Ninan (MKN) *** most probable mechanism function was suggested by comparing the kinetic parameters. The kineticequation for the first stage can be expressed as dα/dt = Aexp(-E/RT)·3(1 - α)^(2/3). Thelifetime equation at mass loss of 10% was deduced as lnτ= -28.7429 + 19797.795/T by isothermalthermogravimetric analysis.

关键词： thermochimica non-isothermal kinetics TG-DTG terbium complex O-methylbenzoate 1,10-phenanthroline

Strong pulses from pulsar PSR J0034-0721

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Research in Astronomy and Astrophysics 2011年第8期11卷 974-980页

作者： Adili Tuoheti Ali Esamdin Hui-Dong Hu Guo-Liang Lv Na Wang Mamat Abliz department of physics Xinjiang University Urumqi 830046 China Xinjiang Astronomical Observatory Chinese Academy of Sciences Urumqi 830011 China Xinjiang university-National Astronomical Observatories Joint Center For Astrophysics Urumqi 830046 China

We present an analysis of strong single pulses from PSR J0034-0721. Our observations were made using the Urumqi 25-m radio telescope at a radio frequency of 1.54 GHz. A total of 353 strong pulses were detected during eight hours of observing, The signal-to-noise ratios of the detected pulses range from 5 to 11.5. The peak fluxes of those pulses are 17 to 39 times that of the average pulse peak. The cumulative distribution of the signal-to-noise ratios of these strong pulses has a rough power-law distribution with a slope of 4.4 q- 0.5. Ten of the strong pulses arrived approximately 23 to 40 ms earlier than the average profile peak. This suggests the possibility that there are two strong pulse-emitting regions.

关键词： pulsars: PSR J0034-0721 individual pulses: strong pulses

Expression of p53 and p21 Protein in Transitional Mucosa Adjacent to Rectal Carcinoma and Its Clinical Implication

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Journal of Huazhong university of Science and Technology(Medical Sciences) 2000年第3期20卷 220-221页

作者：冯茂辉裴锋杨国梁毛哲玉王善福 department of Oncology The Second Affiliated Hospital Hubei Medical University Wuhan department of Surgery Hubei Province Tumor Hospital Wuhan department of Surgery The First Hospital of Huangshi Huangshi

To study the biopathological characteristics of the transitional mucosa adjacent to rectal carcinoma, 34 cases were subjected to mucin histochemical and immunohistochemical study to observe the expression of p53 and p21 protein in distal mucosa adjacent to rectal carcinoma and its relationship to the mucin change. The expression of p53 protein was found in 29. 4 % (10/34) of distal transitional mucosa in the cytoplasm of goblet cells, and its positive staining was within 4 cm from carcinoma margin. A11 p53 positive mucosa was transitional mucosa. Overexpression of p21 protein was found in 26.5 % (9/34) of distal transitional mucosa in cytoplasm of crypt cells, and its positive staining was within 2 cm from carcinoma margin. There was no relationship between the expression of p53 and p21 protein in carcinoma and that in transitional mucosa ( P >0.05). These findings indicated that there was aberrant alteration of p53 and p21 genes in transitional mucosa adjacent to colorectal carcinoma, which provided further evidence that transitional mucosa was an unstable pre cancerous change. The aberrant mucin change and genetic alteration in distal mucosa of rectal cancer is within 4 cm.

关键词： rectal neoplasms intestinal mucosa p53 p21 immunohistochemistry

THE NEAR-CRITICAL AND SUPER-CRITICAL ASYMPTOTIC BEHAVIOR IN THE THERMODYNAMIC LIMIT OF REVERSIBLE RANDOM POLYMERIZATION PROCESSES

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Acta Mathematica Scientia 2000年第3期20卷 380-389页

作者：韩东 Univ Xinjiang Dept Math Urumqi 830046 Peoples R China

In this paper, the near-critical and super-critical asymptotic behavior of a reversible Markov process as a chemical model for polymerization was studied. The results of the present paper, together with an analysis of the sub-critical stage, establish the existence of three distinct stages (sub-critical, near-critical and super-critical stages) of polymerization (in the thermodynamic limit as N --> +infinity,),depending on the value of strength of the fragmentation reaction. These three stages correspond to the size of the largest length of polymers of size N to be itself of order log N, Nm/m+1 (m greater than or equal to 2, m not equal 4n, n greater than or equal to 1) and N, respectively.

关键词： polymerization Markov process limit behavior stationary distribution