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Diversity and ecological distribution of endophytic fungi associated with medicinal plants

Science china(Life Sciences) 2008年第8期51卷 751-759页

作者： SUN JianQiu1,2,3, GUO LiangDong1, ZANG Wei2,3, PING WenXiang4 & CHI DeFu3 1 Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, china 2 college of Life Science and Engineering, Qiqihar university, Qiqihar 161006, china 3 college of forestry, northeast forestry university, harbin 150040, china 4 college of Life Science, Heilongjiang university, harbin 150080, china 1. Systematic Mycology and Lichenology Laboratory Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China 2. college of Life Science and Engineering Qiqihar University Qiqihar 161006 China 3. college of forestry Northeast Forestry University Harbin 150040 China 4. college of Life Science Heilongjiang University Harbin 150080 China

A total of 973 isolates of endophytic fungi were recovered from 1144 tissue fragments of the six me-dicinal plant species belonging to 4 families collected in the Beijing Botanical Garden. Of these isolates 778 sporulated and were identified into 21 taxa by morphological characteristics. Among the taxa 11 belonged to Coelomycetes, 6 to Ascomycetes, and 4 to Hyphomycetes. Various numbers of endophytic fungi (5―8 taxa) were obtained from each plant. Alternaria alternata was the dominant species in the 6 plants, and Microsphaeropsis conielloides was also dominant in Eucommia ulmoides. There were high colonization rates (47.9%―63.1%) and isolation rates (0.7―0.93) of endophytic fungi, and they were conspicuously higher in twigs than those in leaves in the 6 plants examined. The colonization and isolation rates of endophytic fungi increased with the twig age. The results based on the analyses of cluster and Sorenson's similarity coefficients indicated that some endophytic fungi showed a certain degree of host and tissue preference.

关键词： endophytic fungus medicinal plant diversity host and tissue preference

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Method of transforming resistance gene to carbendazim into Trichoderma sp.

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Chinese Science Bulletin 1999年第1期44卷 65-68页

作者： YANG Qian ZHAO Xiaoyancollege of forestry, northeast forestry university, harbin 150040, china harbin Veterinary Institute, harbin 150040, china college of forestry Northeast Forestry University Harbin China harbin Veterinary Institute Harbin China

The method of transforming resistance gene to carbendazim into Trichoderma sp. was studied by using genetic engineering technique. The results show that the time of fungicide selection on the transformed Trichoderma sp. may have very important effect on the resistance gene transformation. By this method, the resistance gene to carbendazim was transformed into Trichoderma harzianum, a strain of T. harzianum with resistance to carbendazim was achieved. The transformant could grow on the medium containing 150μg/mL carbendazim. The resistance is stable after 10 times transfer on non-selective medium.

关键词： gene of resistance to carbendazim gene transformation Trichoderma sp.

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Pictet-Spengler condensation reactions catalyzed by a recyclable H^+-montmorillonite as a heterogeneous BrΦnsted acid

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Science china Chemistry 2010年第3期53卷 561-567页

作者： WANG YuFeng,SONG ZhanBo,CHEN ChunXia & PENG JinSong Department of Chemistry and Chemical Engineering,college of Science,northeast forestry university,harbin 150040,china 1. Department of Chemistry and Chemical Engineering College of Science Northeast Forestry University Harbin 150040 China

Catalytic performance of different cation-exchanged montmorillonite clays has been investigated in the Pictet-Spengler C-C bond forming reaction.H+-Montmorillonite was found to be a very efficient and reusable catalyst for the endo cyclization of β-phenylethylamine derivatives with aldehydes under solvent-free *** addition,an aqueous condensation version for the synthesis of tetrahydro-β-carbolines using the H+-montmorillonite catalyst has been *** these environmentally friendly processes,the use of organic solvents was avoided and the catalyst was recycled with maintenance of high catalytic activity.

关键词： heterogeneous catalysis catalyst recycling mesoporous materials green synthesis

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AN ECOLOGICAL APPROACH TO FOREST HARVESTING

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Journal of northeast forestry university 1990年第1期1卷 99-103页

作者： Shi Jiyannortheast forestry university, harbin, 150040, china 1. northeast forestry university 150040 Harbin China

This paper deals with the relationships between forest harvesting and forest *** basing annual cuts and forest operation plans on ecological *** forest re-sources are decreasing and regeneration is *** combination of forest harvesting with ccologycan be viewed as a solution to the forest harvesting dilemma in *** paper also makes sometechnical recommendations for forest operations based on ecological principles.

关键词： Harvesting Forest ecology Forest operations Logging technique Post harvest regencration

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Comparative study on using carbon or nitrogen limited medium to culture white rot fungi for reactive brilliant red dye K-2BP decolotization under non-sterile conditions

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Science china Chemistry 2007年第5期50卷 718-724页

作者： GAO DaWen1,2, WEN XiangHua1 & QIAN Yi1 1 State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environment Science and Engi- neering, Tsinghua university, Beijing 100084, china 2 School of forestry, northeast forestry university, harbin 150040, china State Key Joint Laboratory of Environmental Simulation and PollutionControl Department of Environment Science and EngineeringTsinghua UniversityBeijing100084China School of forestry Northeast Forestry UniversityHarbin 150040China

In order to explore ways for the application of white rot fungus in dye effluent treatment under non-sterile conditions, experiment on decolorization of reactive brilliant red was carried out, employing nitrogen-limited and carbon-limited medium with C/N ratio of 56/2.2 and 28/44 (in mmol/L), respectively. The results showed that the decolorization rate reached 92% while culturing white rot fungus with ni- trogen-limited medium; however, the decolorization process ended in carbon-limited medium (n(C)/n(N) = 28/44) because of bacterial contamination. In addition, pH rose up to 9.31 after 4 d of decolorization, which was caused by bacterial contamination in the carbon-limited system. Therefore, it is concluded that nitrogen-limited medium can inhibit bacterial growth to some extent while carbon-limited medium is more easily contaminated by bacteria. Nitrogen-limited medium is more suitable in culture of white rot fungus for decolorization of reactive dye. Medium with the ability of inhibiting yeast growth should be developed by adjusting other components of nitrogen-limited medium.

关键词： white rot fungus, Phanerochaete chrysosporium, non-sterile, decolorization, dye

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Recent advances in fermentative biohydrogen production

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Progress in Natural Science:Materials International 2008年第3期18卷 253-258页

作者： Xuemei Liu a,b, Nanqi Ren a, Funan Song b, Chuanping Yang b, Aijie Wang a a harbin Institute of Technology, harbin 150010, china b northeast forestry university, harbin 150040, china harbin Institute of Technology Harbin 150010 China northeast forestry university Harbin 150040 China

Hydrogen energy, as a kind of clean energy with great potential, has been a hotspot for study worldwide. Based on the recent research on biohydrogen production, this paper gives a brief review on the following aspects: fermentative hydrogen production process and the engineering control statagy, key factors affecting the effciency of hydrogen production, such as substrates, cysteine, metal ions, anaerobic fermentation terminal products, and formic acid and ammonia. Moreover, anaerobic fermentative hydrogen-producing strain and reg- ulation and control of enzyme gene in fermentative hydrogen production are also discussed. Finally, the prospect of anaerobic fermen- tative biohydrogen production is proposed in three study areas, namely developing new techniques for breeding hydrogen-producing bacteria, exploitations of more strains and gene resources, and intensifying the application of microbial molecular breeding in hydrogen production.

关键词： Fermentation Biohydrogen production Regulation and control Novel strains

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WATER TRANSPORT IN WOOD PARTICLE MATERIAL

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Journal of northeast forestry university 1990年第1期1卷 79-85页

作者： Shang Dckunortheast forestry university, harbin 150040, P.R.china 1. northeast forestry university 150040 Harbin P.R.China

An analogy between water flux and the field of temperature gradient in a column of wood fuel chips was discovered by these *** heat-mass transfer coeffieient was deter-mined by least squares *** equation relating water flux and tempetature gradient was devel-opcd and it was demonstrated that the tempeature gradient was the main driving force for *** meehanism was evaporation-condensation of water vaor through natural convcctionin air *** study gives a theoretical exlanation for analyzing the problem of water transport inwood particle material.

关键词： Wood fuel chips Water flux Temperature gradient Analogy

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OAKS(QUERCUS SPP.)IN northeast china

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Journal of northeast forestry university 1990年第1期1卷 1-10页

作者： Chen Dakenortheast forestry university, harbin 150040, P. R. china 1. northeast forestry university 150040 Harbin P. R. China

There arc eight species and four varieties of oak in northeast china. This paper reports and describes the distribution and phylocoenogenesis of the Korean pine-broadleaved mixed forest and the mongolian oak formation. The close relationship between the flora of Ussuli—Amur region and Japan is discussed. Analyses of heat and energy balance, and biomass of mongolian oak forests arc provided. Many measurements indicate that, in a nonclosed stand, the wood production of a stand is positively correlated with leaf area index (LAI).

关键词： Oak Phytococnogenesis Biomass Heat and energy balance

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Effect of Anti-freezing Admixtures on Alkali-silica Reaction in Mortars

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Journal of Wuhan university of Technology(Materials Science) 2005年第2期20卷 80-82页

作者：柳俊哲 School of Civil Engineering Northeast Forestry University Harbin 150040 China Assoc.Prof

The influence of anti-freezing admixture on the alkali aggregate reaction in mortar was analyzed with accelerated methods. It is confirmed that the addition of sodium salt ingredients of anti-freezing admixture accelerates the alkali silica reaction to some extent, whereas calcium salt ingredient of anti-freezing admixture reduces the expansion of alkali silica reaction caused by high alkali cement. It is found that the addition of the fly ash considerably suppresses the expansion of alkali silica reaction induced by the anti-freezing admixtures.

关键词： anti-freezing admixture alkali-silica reaction reactive aggregates fly ash

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AGE STRUCTURE AND SPATIAL PATTERN OF OLD-GROWTH KOREAN PINE FOREST IN XIAOXING'ANLING MOUNTAIN

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Journal of northeast forestry university 1991年第2期2卷 17-22页

作者： Ge Jianping Guo Haiyan Chen Dong northeast forestry university, harbin 1. northeast forestry university Harbin

Spatial pattern of trees, basal stem increment and height were examined in a 5.0 ha mixed deciduous/ Pinus koraiensis forest from October in 1984 to May in 1987. Conclusions arc as following: 1. Analysis of the age-structure of the korcan pine forest shows that it is different from general steady-state forest type. The lack of saplings and individuals in small-diameter class clearly indicates that regeneration is not continuous. Continuous regeneration depends on thinning of the canopy to form a gap. Similar-aged korcan pine seedings grow in these gaps. 2. The horizontal structure of the korcan pine is a mosaic of more or less even-aged groups of trees. The mosaic is a result of korcan pine by regeneration strategy. The aggregations of korcan pine of different ages overlaps to form a continuous population generation. As a results, the population is maintained in steady state.

关键词： Xiaoxing'anling Age structure Spatial pattern Population Old-growth korean pine forest

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