Comparative study on using carbon or nitrogen limited medium to culture white rot fungi for reactive brilliant red dye K-2BP decolotization under non-sterile conditions

作     者：GAO DaWen1,2, WEN XiangHua1 & QIAN Yi1 1 State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environment Science and Engi- neering, Tsinghua University, Beijing 100084, China 2 School of Forestry, Northeast Forestry University, Harbin 150040, China 

作者机构：State Key Joint Laboratory of Environmental Simulation and Pollution Control Department of Environment Science and Engineering Tsinghua University Beijing China School of Forestry Northeast Forestry University Harbin China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2007年第50卷第5期

页      面：718-724页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 50478010) China Postdoctoral Research Foundation (Grant No. 20040350022) 

主　　题：white rot fungus, Phanerochaete chrysosporium, non-sterile, decolorization, dye 

摘      要：In order to explore ways for the application of white rot fungus in dye effluent treatment under non-sterile conditions, experiment on decolorization of reactive brilliant red was carried out, employing nitrogen-limited and carbon-limited medium with C/N ratio of 56/2.2 and 28/44 (in mmol/L), respectively. The results showed that the decolorization rate reached 92% while culturing white rot fungus with ni- trogen-limited medium; however, the decolorization process ended in carbon-limited medium (n(C)/n(N) = 28/44) because of bacterial contamination. In addition, pH rose up to 9.31 after 4 d of decolorization, which was caused by bacterial contamination in the carbon-limited system. Therefore, it is concluded that nitrogen-limited medium can inhibit bacterial growth to some extent while carbon-limited medium is more easily contaminated by bacteria. Nitrogen-limited medium is more suitable in culture of white rot fungus for decolorization of reactive dye. Medium with the ability of inhibiting yeast growth should be developed by adjusting other components of nitrogen-limited medium.