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Journal of Integrative agriculture 2024年第3期23卷 863-875页

作者： hainan Liu Maosong Pei Charles Ampomah-Dwamena Yaxin Shang Yihe Yu Tonglu Wei Qiaofang Shi Dalong Guo college of Horticulture and Plant Protection Henan University of Science and TechnologyLuoyang 471000China Henan Engineering Technology Research Center of Quality Regulation and Control of Horticultural Plants Luoyang 471000China The New Zealand Institute for Plant&Food Research Limited(PFR) Auckland 1010New Zealand

The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated *** study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.

关键词： grape postharvest softening folic acid alternative splicing Pectinesterase 2 alternative 3'splice site(A3SS)

Identification,characterization,and verification of miR399 target gene in grape

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Horticultural Plant Journal 2024年第1期10卷 91-102页

作者： Maosong Pei hainan Liu Tonglu Wei Huiying Jin Yihe Yu Mengting Ma Xiaomeng Song Rundong Dai Dalong Guo Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural Plants College of Horticulture and Plant ProtectionHenan University of Science and TechnologyLuoyangHenan 471023China

The microRNA miR399 plays an important role in phosphorus signal transduction pathways in ***,miR399 was shown to be closely associated with berry ripening in grape(Vitis vinifera).The objective of the present study was to elucidate the evolutionary characteristics of the miR399 gene family in grape and to verify the cleavage effect on the target *** miR399s were identified by miRNA sequencing and retrieval from the miRBase *** mature sequences and precursor sequences were subjected to phylogenetic analysis to reconstruct evolutionary trees,as well as secondary structure analysis of the precursor sequence,and prediction of target *** cisacting elements in the miR399 promoter were predicted and the cleavage effect of grape miR399b on its target genes was *** grape miR399 family comprised nine precursor sequences and nine mature *** precursor sequences formed a typical and stable stem—loop *** minimum folding free energy ranged from-55.70 kcal·mol^(-1)to-37.40 kcal·mol^(-1).Multiple sequence alignment revealed that the miR399 family was highly *** grape miR399 family was phylogenetically closely related to peach,apple,and citrus *** miR399s were predicted to target inorganic phosphate transporter 1—3,phospholipase D delta-like,and *** cleavage effect of grape miR399b on the target genes was verified by means of a dual-luciferase assay and 5’*** GUS staining showed that the promoter activity of miR399b was promoted by GA3treatment.

关键词： Grape miR399 Phosphorus metabolism Early ripening

Application of CRISPR/Cas12i.3 for targeted mutagenesis in broomcorn millet(Panicum miliaceum L.)

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Journal of Integrative Plant Biology 2024年第8期66卷 1544-1547页

作者： Yuhe Bai Shengnan Liu Yan Bai Zhisong Xu hainan Zhao Haiming Zhao Jinsheng Lai Ya Liu Weibin Song State Key Laboratory of Maize Bio-breeding Key Laboratory of Genome Editing Research and ApplicationMinistry of Agriculture and Rural AffairsDepartment of Plant Genetics and BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China National Agricultural Technology Extension&Service Center Beijing 100125China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decre... 详细信息

关键词： arable continue raise

The frequencies of high frequency quasi-periodic oscillations in two different frames in black hole LMXBs

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Research in Astronomy and Astrophysics 2011年第11期11卷 1327-1335页

作者： Chang-Sheng Shi college of Material science and Chemical Engineering,hainan university,hainan 570228,china.Key Laboratory of Modern Astronomy and Astrophysics,Nanjing university,Nanjing 210093,china college of Material science and Chemical Engineering Hainan University Hainan 570228 China Key Laboratory of Modern Astronomy and Astrophysics Nanjing University Nanjing 210093 China

We discuss the modes of the Alfvén waves in the accretion disk with a toroidal magnetic field in black hole low mass X-ray binaries in a rotating frame. By solving the perturbed general relativistic magnetohydrodynamic equations in the rotating frame, we find two stable modes of the Alfvén wave which are the same as those in the fiducial observer frame. This gives a feasible way to transform between the two different frames, which validates the possible Alfvén wave modes in the accreting celestial bodies with a toroidal magnetic field.

关键词： accretion accretion disks-magnetohydrodynamics-relativity

A characterization for a graphic sequence to be potentially C_r-graphic

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science china.Mathematics 2010年第11期53卷 2893-2905页

作者： YIN JianHua Department of Mathematics, college of Information science and Technology, hainan university, Haikou 570228, china Department of Mathematics College of Information Science and Technology Hainan University Haikou China

Let r 3, n r and π = (d1, d2, . . . , dn) be a graphic sequence. If there exists a simple graph G on n vertices having degree sequence π such that G contains Cr (a cycle of length r) as a subgraph, then π is said to be potentially Cr-graphic. Li and Yin (2004) posed the following problem: characterize π = (d1, d2, . . . , dn) such that π is potentially Cr-graphic for r 3 and n r. Rao and Rao (1972) and Kundu (1973) answered this problem for the case of n = r. In this paper, this problem is solved completely.

关键词： graph cycle degree sequence

Effect of lead on survival, locomotion and sperm morphology of Asian earthworm, Pheretima guillelmi

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Journal of Environmental sciences 2009年第5期21卷 691-695页

作者： ZHENG Rongquan, LI Canyang college of Chemistry and life science, Zhejiang Normal university, Jinhua 321004, china. college of Chemistry and life science Zhejiang Normal University Jinhua 321004 China

To provide basic toxicity data for formulating risk characterization benchmarks, the effects of lead on survival, locomotion, and sperm morphology were investigated in the Asian earthworm Pheretima guillelmi. The LC50 of P. guiUelmi for 7 and 14 d were 4285＋339 mg/kg and 3207＋248 mg/kg, which shows P. guillelmi can tolerate a higher concentration of lead nitrate. The average weight of the surviving earthworms decreased at concentration of 2800 mg Pb/kg soil, and the locomotor ability of earthworms exposed to a range of soil Pb concentrations showed a general decrease with increasing Pb concentrations. We also presented data depicting the sperm morphology of earthworms, which shows potential as a sensitive biomarker for measuring the effects of heavy metal on reproduction.

关键词： lead Pheretima guillelmi sperm morphology locomotion

Predicting neutron star spins from twin kHz QPOs

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science china.Physics,Mechanics & Astronomy) 2010年第S1期53卷 247-248页

作者： SHI ChangSheng Department of Astronomy,Nanjing university,Nanjing 210093,china.college of Material science and Chemical Engineering,hainan university,hainan 570228,china Department of Astronomy Nanjing University Nanjing China

We briefly review the proposed relations between the frequencies of twin kilohertz quasi-periodic oscillations(kHz QPOs) and the spin frequencies in neutron star low-mass X-ray binaries(NSLMXBs).To test the validity of the proposed models,we estimate the spin frequencies under these theoretical relations and compare them with the measured *** seems that magnetohydrodynamic(MHD) oscillations are more promising to account for the kHz QPOs.

关键词： win kHz QPOs spin neutron stars LMXBs

Cloning of a Homolog of Ripening-Reponsive Gene from Banana(Musa accuminata AAA) Fruit

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Cloning of a Homolog of Ripening-Reponsive Gene from Banana(...

Effects of chronic exposure to nonylphenol on locomotor activity and social behavior in zebrafish(Danio rerio)

2009整合植物生物学研讨会

作者： Jiashui Wang~(1,2),Fan Liu~1,Biyu Xu~1 and Zhiqiang Jin~(1,3*) 1 Key Laboratory of Tropical Crop Biotechnology,Ministry of agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural sciences, hainan 571101,china.2 college of life and agriculture science,hainan university,hainan 571101,china.3 Haikou Experimental Station,CATAS,571102,hainan,china.

To investigate the mechanism of postharvest banana ripening at molecular levels,a cDNA fragment was obtained from cDNA library of postharvest banana(Musa accuminata L. AAA group)*** cDNA showed up-regulated in early stage of banana *** determine the role of the cDNA in postharvest ripening banana,the full lengh of this cDNA

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Journal of Environmental sciences 2010年第9期22卷 1435-1440页

作者： Jigang Xia,Cuijuan Niu ,Xuejiao Pei Ministry of Education Key Laboratory of Biodiversity science and Ecological Engineering,college of life sciences,Beijing Normal university,100875 Beijing,china. Ministry of Education Key Laboratory of Biodiversity science and Ecological Engineering College of Life Sciences Beijing Normal University 100875 Beijing China

Nonylphenol （NP）, a xenoestrogen ubiquitously found in aquatic ecosystems, is of high environmental concern. The present work assessed the effects of exposure to NP on locomotor activity and social behavior in male and female zebrafish （Danio rerio）, to evaluate the possible hazard of NP to fish behavior and to pursue a potential biomarker of NP contamination. Fish were randomly divided into six groups. Five groups were receiving 0.1, 1, 10, 50 and 100 μg/L nominal concentrations of NP for 60 days, respectively. A sixth control group was given the same treatment as the other five groups, but no NP. Locomotor activity, aggressive behavior, group preference and leaving shoal were examined. NP exposure showed marked influence on locomotor activity of the male zebrafish, whereas that of the female was not significantly affected by NP. Aggressive behavior and group preference were significantly affected by NP exposure in both male and female groups. Locomotor activity and aggressive behavior of the male and group preference of both male and female zebrafish were clearly inhibited at 100 μg/L NP. No significant alteration in leaving shoal was observed under NP exposure. The results suggested that changes in locomotor activity and aggressive behavior of the male and group preference of both male and female may be used as an ecologically relevant integrative biomarker of NP contamination.

关键词： chronic toxicity nonylphenol biomarker zebrafish