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Science Bulletin 2012年第Z2期 3782-3789页

作者： DONG HuiNa 1,2,4,5,ZHAO XueMing 2,4,5,MA YuanYuan 1* & ZHANG MinHua 1,3 1 Tianjin University R&D center for Petrochemical Technology,Tianjin University,Tianjin 300072,China 2 Department of Biochemical Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China 3 State Key Laboratory of Engines,Tianjin University,Tianjin 300072,China 4 Key Laboratory of systems Bioengineering,Ministry of Education,Tianjin University,Tianjin 300072,China 5 Edinburgh-Tianjin Joint Research Centre for systems biology and Synthetic biology,Tianjin University,Tianjin 300072,China

A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman(PB) design and response surface methodology(RSM).The effects of 19 medium components were investigated by PB *** of these components were determined to have significant effects on ethanol *** statistical model was constructed via central composite design(CCD) using five selected variables,including xylose,trisodium citrate,choline chloride,pyridoxine,and *** validity of the developed model was *** ethanol concentration in the optimized medium was 6.7% higher than in the RM *** optimized medium was then simplified to give the final synthetic medium(S2),in which the ethanol concentration was 20.7% higher than in the RM *** and trisodium citrate were the key medium components influencing ethanol production,while calcium pantothenate was the only growth factor required by recombinant *** production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.

关键词： recombinant Zymomonas mobilis synthetic medium ethanol production response surface methodology trisodium citrate xylose

Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis

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Cell Research 2003年第3期13卷 171-177页

作者： SHENG WEI ZOU,JIAN CHAO ZHANG,XIAO DONG ZHANG,SHI YING MIAO,SHU DONG ZONG,QI SHENG,LIN FANG WANGNational Laboratory of Medical Molecular biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,Peking Union Medical College,5 Dong Dan 3-tiao,Beijing 100005,ChinaNational B,esearch Institute for Family Planing,WHO Collaboration center for B,esearch in Human Reproduction,12 Da Hui Si,Beijing 100081,China 1National Laboratory of Medical Molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China 2National Research Institute for Family Planing WHO Collaboration Center for Research in Human Reproduction 12 Da Hui Si Beijing 100081 China 1National Laboratory of Medical Molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China 1National Laboratory of Medical Molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China 2National Research Institute for Family Planing WHO Collaboration Center for Research in Human Reproduction 12 Da Hui Si Beijing 100081 China 1National Laboratory of Medical Molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China 1National Laboratory of Medical Molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China

Variable Charge X/Y(VCX/Y)is human testis-specific gene family that localized on X and Y *** this study,VCY protein was expressed in *** in the form of glutathione-S-transferase(GST)fusion *** the purified fusion protein as antigen,the anti-GST-VCY antibody was generated andthe localization of VCY protein in human testis was determined by *** the testisseminiferous epithelium,VCY proteins were highly expressed in nuclei of germ *** propidium io-dide staining and green fluorescent protein(GFP)tag technologies,VCY and VCX-8r proteins were mainlylocalized in the nucleoli of COS7 *** *** colocalization for VCY and VCX-8r in COS7 cellswas also *** VCY cDNA as bait,a cDNA fragment of acidic ribosomal protein PO was obtainedusing yeast two-hybrid *** the information above indicated that VCX/Y protein family might beinvolved in the regulation of ribosome assembly during spermatogenesis.

关键词： VCX/Y protein spermatogenesis nucleoli colocnlization.

Effects of disinhibition on spatiotemporal pattern of neuronal first recruitment in neuronal networks

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Progress in Natural Science:Materials International 2009年第5期19卷 615-621页

作者： Liangbin Pana,b,c,1, Xindong Songa,b,c,1, Guangxin Xiang a,b,c, Jing Zhua,b,c, Jing Cheng a,b,c,d,a Medical systems biology Research center, Tsinghua University School of Medicine, Beijing 100084, China b Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China c National Engineering Research center for Beijing Biochip Technology, 18 Life Science Parkway, Beijing 102206, China d State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China [a]Medical systems biology Research center Tsinghua University School of Medicine Beijing 100084 China [b]Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China [c]National Engineering Research Center for Beijing Biochip Technology 18 Life Science Parkway Beijing 102206 China [d]State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing 100084 China

The propagation of neuronal activities is a key feature to understanding information processing in networks. The analysis based on first-spikes of bursts in turn plays an important role in the research of neuronal activity propagation. Our focus here is to investigate how spatiotemporal patterns of neuronal first-spikes are affected by disinhibition. Multi-electrode arrays were used to record stimulation-evoked bursts of multiple neurons in randomly cultured neuronal networks. Both the precise timing of and the rank relationships between first-spikes were analyzed. Compared with evoked bursts in the network’s native state, the precise first-spike latencies in its dis-inhibited state are more consistent and the propagation of its bursting activities is much faster. Additional points of interest are that disinhibited neuronal networks can be evoked to generate stable and distinguishable neuronal first recruitment spatiotemporal patterns specific to the stimulation site, and that the disinhibition may cause the original spatiotemporal patterns to change in a heterogeneous manner with regards to different propagation pathways.

关键词： Neuronal recruitment Spatiotemporal pattern Disinhibition Network Multi-electrode array

Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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Science China(Life Sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China 2 Department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,China 3 College of Life Science,Capital Normal University,Beijing 100037,China 4 College of Life Science,Zhongkai University of Agriculture and Technology,Guangzhou 510225,China 5 College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,China 6 Department of biology,Henan Institute of Science and Technology,Xinxiang 453003,China 1. Beijing Agro-Biotechnology Research center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan 411104 China 3. College of Life Science Capital Normal University Beijing 100037 China 4. College of Life Science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. College of Grassland Science Gansu Agricultural University Lanzhou 730070 China 6. Department of biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

Predicting Polymerase Ⅱ Core Promoters by Cooperating Transcription Factor Binding Sites in Eukaryotic Genes

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 250-258页

作者： Xiao-Tu MA, Min-Ping QIAN*, and Hai-Xu TANG1 School of Mathematical Sciences, Peking University and center for Theoretical biology, Peking University, Beijing 100871, China 1Department of Computer Science and Engineering, University of California, San Diego, La Jolla CA 92093-0114, USA School of Mathematical Sciences Peking University and Center for Theoretical Biology Peking UniversityBeijing 100871 China Department of Computer Science and Engineering University of CaliforniaSan Diego La Jolla CA 92093-0114 USA

Several discriminate functions for predicting core promoters that based on the potential cooperation between transcription factor binding sites (TFBSs) are discussed. It is demonstrated that the promoter predicting accuracy is improved when the cooperation among TFBSs is taken into consideration. The core promoter region of a newly discovered gene CKLFSF1 is predicted to locate more than 1.5 kb far away from the 5' end of the transcript and in the last intron of its upstream gene, which is experimentally confirmed later. The core promoters of 3402 human RefSeq sequences, obtained by extending the mRNAs in human genome sequences, are predicted by our algorithm, and there are about 60% of the predicted core promoters locating within the ± 500 bp region relative to the annotated transcription start site.

关键词： promoter transcription factor binding site transcription start site gene finding

Expression of Monomeric Insulin Precursor in Pichia pastoris and Its Conversion into Monomeric B27 Lys Destripeptide Insulin by Tryptic Hydrolysis

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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 234-240页

作者： Jin-Guo DING~(1,3) Jian FEI~2 Da-Fu CUI~1 You-Shang ZHANG~1 ~1Institute of Biochemistry and Cell biology,and ~2Model Organism Research center,Shanghai Institutes for Biologicol Sciences, Chinese Academy of Sciences,Shanghai 200031,China ~3Graduate School of the Chinese Academy of Sciences,Shanghai 200031,China Institute of Biochemistry and Cell biology Graduate School of the Chinese Academy of Sciences Shanghai 200031 China

Monomeric B27 Lys destripeptide insulin (B27 Lys DTrI) was designed and produced from itsprecursor expressed in Pichia pastoris through tryptic hydrolysis instead of the less efficient *** monomeric B27 Lys DTrI precursor (MIP) was purified from a cultured medium *** by a combination of hydrophobic,size-exclusion,and ion-exchange *** purifiedMIP was converted,by tryptic hydrolysis,to B27 Lys DTrI,which was then purified by ion-exchangechromatography to homogeneity as assessed by native gel electrophoresis,HPLC,amino acid composition,and electrospray mass-spectrometric analysis.B27 Lys DTrI exhibited superior monomeric properties insize-exclusion *** yield of MIP was 200 mg per liter of culture,and the overall yield ofpurified B27 Lys DTrI from the crude MIP was 70%.The in vivo biological activity of B27 Lys DTrI asdetermined by the mouse convulsion assay was 21 U/mg,identical to that obtained by semisynthesis.

关键词： monomeric B27 Lys destripeptide insulin tryptic hydrolysis Pichia pastoris

An improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data

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Protein & Cell 2015年第12期6卷 919-923页

作者： Jia Wang (1) (2) (3) Weiguang Wang (1) (2) Wen Song (2) (3) (4) Zhifu Han (2) (3) (4) Heqiao Zhang (2) (3) (4) Jijie Chai (2) (3) (4) Hongwei Wang (2) (3) (4) Jiawei Wang (1) (2) 1.State Key Laboratory of Membrane biology School of Life Sciences Tsinghua University Beijing 100084 China 2.Center for Structural Biology School of Life Sciences Tsinghua University Beijing 100084 China 3.Tsinghua-Peking Center for Life Sciences School of Life Sciences Tsinghua University Beijing 100084 China 4.Ministry of Education Key Laboratory of Protein Science School of Life Sciences Tsinghua University Beijing 100084 China

Cryo-electron microscopy （cryo-EM） and macromolecular crystallography （MX） both allow the creation of three-dimensional images of biological macromolecules. EM data usually provide access to three-dimensional reconstructions of large biological complexes at low resolution, whereas MX usually delivers atomic or quasi-atomic resolution structures （1-3 A）.

关键词： Phase Error Molecular Replacement Histogram Match TLR13 Molecule Solvent Flatten

Canon-π Interactions. Synthesis and Crystal Structure of Palladium Complex:[K(DB18C6)]_2[Pd(SCN)_4]

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Chinese Chemical Letters 2000年第5期11卷 463-466页

作者： Jian Min DOU Ying LIU Lan Ying ZHU De Zhi SUN Pei Ju ZHENGZ Chen Xia DU Yu ZHU (Department of Chemistry and biology, Liaochcng Teacher’s University, Liaocheng 252000 Research center of Analysis and Measurement Fudan University, Shanghai 200433 ~3Key La Department of Chemistry and biology Liaocheng Teacher's University Liaocheng 252000 China Res. Ctr. of Anal. and Measurement Fudan University Shanghai 200433 China Key Laboratory of Applied Chemistry Zhengzhou University Zhengzhou 450052 China

A novel Pd(Ⅱ)-dibenzo-18-crown-6(DB18C6) complex [K(DB18C6)]_2, [Pd(SCN)_4] has been isolated and characterized by X-ray diffraction analysis. In the solid state, it displays a quasi- one-dimensional infinite chain of two [K(DB18C6)]+ complex canons and a [Pd(sCN)_4]^(2-) anion bridged by K+-π interactions between adjacent [K(DB18C6)] + units.

关键词： Canon-π interacions crown ether bestal Structure palladium complex.

CONSTRUCTION OF Sesbania rostrata COSMID GENOMIC LIBRARY AND MOLECULAR CLONING OF LEGHEMOGLOBIN GENE

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Science China Chemistry 1989年第12期32卷 1457-1464页

作者：刘阳范云六 Laboratory of Molecular biology. Biotechnology Research center Chinese Academy of Agricultural Sciences Beijing 100081 PRC

Using plant mini-Ti cosmid pEND4K, the cosmid genomic library of Sesbania rostratawas constructed. Sizes of plant DNA inserts in clones were 25- 33 kb. The restrictionmapping showed that different recombinant involved various types of plant DNA insert. 4clones containing leghemoglobin gene sequence of S. rostrata were obtained by in situ hy-bridization of colonies. The cloning of leghemoglobin gene sequence has been confirmedby plasmid DNA dot hybridization and Southern blot hybridization.

关键词： Sesbania rostrata plant cosmid genomic library leghemoglobin gene molecular cloning

psRobot:a web-based meta-analysis toolbox for plant small RNA

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psRobot:a web-based meta-analysis toolbox for plant small RN...