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Characterization of Function of Three Domains in Dishevelled-1: DEP Domain is Responsible for Membrane Translocation of Dishevelled-1

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Cell Research 2004年第4期14卷 324-330页

作者： Wei Jun PAN, Shu Zhao PANG, Tao HUANG, Hui Yun GUO, Dianqing WU, Lin LI Laboratory of Molecular Cell biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, 320 Yueyang Rd, Shanghai 200031 College of Life Science, Jilin University, Changchun 130061, China. Department of Genetics and Developmental biology, University of Connecticut Health center, Farmington, Connecticut 06030, USA. 1Laboratory of Molecular Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031. 2College of Life Science Jilin University Changchun 130061 China. 1Laboratory of Molecular Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031. 2College of Life Science Jilin University Changchun 130061 China. 3Department of Genetics and Developmental biology University of Connecticut Health Center Farmington Connecticut 06030 USA. 1Laboratory of Molecular Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031.

Wnt signaling plays an important role in embryogenesis and tumorgenesis. Although the mechanism about how Wnts transduce their signaling from receptor frizzled (Fz) to cytosol has not been understood, dishevelled (Dvl) protein was considered as the intersection of Wnt signal traffic. In this study, we characterized the function of three domains (DIX, PDZ and DEP) of Dvl-1 in canonical Wnt signal transduction and Dvl-1 membrane translocation. It was found both DIX and DEP domain were sufficient to block Wnt-3a-induced LEF-1 transcriptional activity and free cytosol β-catenin accumulation; whereas PDZ domain and a functional mutant form of DEP domain (DEP-KM) had no effect on canonical Wnt signaling. In addition, when cotransfected with Fz-7, DEP domain, but not DIX, PDZ or DEP-KM, translocated and co-localized with Fz-7 to the plasma membrane, which was similar to Dvl-1. Furthermore, it was DEP domain that could block Fz-7-induced membrane translocation of Dvl-1 via a possible competitive mechanism. Thes

关键词： Wnt frizzled-7 dishevelled DIX PDZ DEP membrane translocation signal transduction

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Genetic regulation of programmed cell death in Drosophila

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Cell Research 2000年第3期10卷 193-204页

作者： LEE CHENG-YU, ERIC H BAEHRECKE (l center for Agricultural Biotechnology, University of Maryland Biotechnology institute, College Park, Maryland 20742,USA Department of biology, University of Maryland, College Park, Maryland 20742, USA) centerforAgriculturalBiotechnology UniversityofMarylandBiotechnologyInstituteDepartmentofBiologyUniversityofMarylandCollegePark20742USA.

Programmed cell death plays an important role in maintaining homeostasis during animal development, and has been conserved in animals as different as nematodes and humans. Recent studies of Drosophila have provided valuable information toward our understanding of genetic regulation of death. Different signals trigger the novel death regulators rpr, hid, and grim, that utilize the evolutionarily conserved iap and ark genes to modulate caspase function. Subsequent removal of dying cells also appears to be accomplished by conserved mechanisms. The similarity between Drosophila and human in cell death signaling pathways illustrate the promise of fruit mes as a model system to elucidate the mechanisms underlying regulation of programmed cell death.

关键词： Apoptosis autophagy phagocytosis development.

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Cell surface activation of progelatinase A (proMMP-2) and cell migration

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Cell Research 1998年第3期8卷 179-186页

作者： NAGASE HIDEAKI（Department of Biochemistry and Molecular biology,University of Kansas Medical center, 3901 Rainbow Blvd,Kansas City, KS 66160. E-mail: hnagase@kumc.edu） Department of Biochemistry and Molecular biology University of Kansas Medical Center 3901 Rainbow Blvd Kansas City KS 66160

Gelatinase A (MMP-2) is considered to play a critical role in cell migration and invasion. The proteinase is secreted from the cell as an inactive zymogen. In vivo it is postulated that activation of progelationase A (proMMP-2) takes place on the cell surface mediated by membrane-type matrix metalloproteinases (MT-MMPs). Recent studies have demonstrated that proMMP-2 is recruited to the cell surface by interacting with tissue inhibitor of metalloproteinases-2 (TIMP-2) bound to MT1MMP by forming a ternary complex. bee MT1-MMP closely located to the ternary complex then activates proMMP-2 on the cell surface. MT1-MMP is found in cultured invasive cancer cells at the invadopodia. The MTMMP/TIMP-2/ MMP- 2 system t bus provides localized expression of proteolysis of the extracellular matrix required for cell migration.

关键词： GelatinaseA MT-MMPs Cell surface activation TIMP-2 Extracellular matrix

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Treatment with Vector-expressed Small Hairpin RNAs against Ki67 RNA-induced Cell Growth Inhibition and Apoptosis in Human Renal Carcinoma Cells

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 254-261页

作者： Jun-Nian ZHENG~(1,3*) Ya-Feng SUN~(2#) Dong-Sheng PEI~2 Jun-Jie LIU~1 Teng-Xiang MA~3 Rui-Fa HAN~3 Wang LI~1 Dian-Bao ZHENG~1 Jia-Cun CHEN~1 Xiao-Qing SUN~1 1 Laboratory of Urology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,China 2 Research center for Biochemistry and Molecular biology,Xuzhou Medical College,Xuzhou 221002,China 3 Institute of Urology,Medical University of Tianjin,Tianjin 300211,China LaboratoryofUrology AffiliatedHospitalofXuzhouMedicalCollegeXuzhou221002China ResearchcenterforBiochemistryandMolecularbiology XuzhouMedicalCollegeXuzhou221002China InstituteofUrology MedicalUniversityofTianjin

Short hairpin RNAs (shRNAs) transcribed *** polymerase Ⅲ promoters can triggersequence-selective gene silencing in mammalian *** virtue of their excellent function in knocking downexpression of cancer-associated genes,shRNAs could be used as new therapeutic agents for *** of Ki67 in renal cancer has been correlated to a more aggressive tumor phenotype,inhibitionof Ki67 protein expression by means of shRNAs seems to be a promising approach for the therapy of *** this study,we constructed an expression plasmid encoding shRNAs against the Ki67 gene,namedpSilencerKi67,and transfected it into human renal carcinoma *** pSilencerKi67 was shown to signifi-cantly knock down the expression of the Ki67 gene in human renal carcinoma cells,resulting in inhibitingproliferation and inducing apoptotic cell death that can be maintained for at least *** findings offer thepromise of using vector-based shRNAs against Ki67 in renal cancer gene therapy.

关键词： Ki67 gene shRNAs renal cell carcinoma proliferation apoptosis

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Outsmarting cancer:an international brainstorm in Guangzhou

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Chinese Journal of Cancer 2011年第8期30卷 505-507页

作者： Chao-Nan Qian 1,2 and Wei Zhang 31 Laboratory of Cancer and Developmental Cell biology,Van Andel Research Institute,Grand Rapids,MI 49503,USA 2 State Key Laboratory of Oncology in South China,Sun Yat-sen University Cancer center,Guangzhou,Guangdong 510060,P.R.China 3 Department of Pathology,University of Texas MD Anderson Cancer center,Huston,TX 77030,USA. Laboratory of Cancer and Developmental Cell biology Van Andel Research Institute Grand Rapids MI 49503 USA. qianchn@***

Cancer is the most deadly disease in the United *** developing countries such as China, cancer is increasingly prevalent as a cause of *** “war against cancer” that was initially declared in the United States has become a global war that requires an alliance of world-wide cancer *** part of such an effort, the Second Guangzhou International Symposium on Oncology was held on May 20-22, 2011, in Guangzhou, China.

关键词：抗癌症广州国际发展中国家研究人员肿瘤学美国战争

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Inhibition of Proliferation and Induction of Apoptosis in Human Renal Carcinoma Cells by Anti-telomerase Small Interfering RNAs

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 500-506页

作者： Jun-Nian ZHENG~(1,3#*)Ya-Feng SUN~(2#)Dong-Sheng PEI~2 Jun-Jie LIU~1 Jia-Cun CHEN~1 Wang LI~1 Xiao-Qing SUN~1 Qi-Duo SHI~3 Rui-Fa HAN~3 Teng-Xiang MA~3 1 Laboratory of Urology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,China 2 Research center for Biochemistry and Molecular biology,Xuzhou Medical College,Xuzhou 221002,China 3 Institute of Urology,Tianjin Medical University,Tianjin 300070,China LaboratoryofUrology AffiliatedHospitalofXuzhouMedicalCollegeXuzhou221002China ResearchcenterforBiochemist'ryandMolecularbiology XuzhouMedicalCollegeXuzhou221002China

Telomerase is an attractive molecular target for cancer therapy because it is present in mostmalignant cells but is undetectable in most normal somatic *** telomerase consists of two subunits,an RNA component(hTR)and a human telomerase reverse transcriptase component(hTERT).Smallinterfering RNA(siRNA),one kind of RNA interferences,has been demonstrated to be an effective methodto inhibit target gene expression in human *** investigated the effects of siRNA targeting at both hTRand hTERT mRNA on the inhibition of telomerase activity in human renal carcinoma cells(HRCCs).Theproliferation and apoptosis of HRCCs were *** treatment of HRCCs using hTR and hTERTsiRNAs resulted in significant decrease of hTR mRNA,hTERT mRNA and hTERT *** siRNA canalso inhibit the telomerase activity and the proliferation of ***,they can induce apoptotic celldeath in a dose-dependent *** these findings,we propose that the inhibition of telomerase activityusing siRNA targeting hTR and hTERT might be a rational approach in renal cancer therapy.

关键词： hTERT hTR telomerase siRNA renal cell carcinoma proliferation apoptosis

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Spatio-temporate Expression of P53, Bcl-2 and Fas in the Retina of RD, Rds and C3H Mice

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眼科学报 2000年第3期16卷 158-162页

作者： Ying Chen, Qingjiong ZhangOcular Genetic & Molecular biology, Zhongshan Ophthalmic center, Sun Yat-sen University of Medical Sciences Guangzhou 510060, China Ocular Genetic & Molecular Biology Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

PURPOSE: To investigate the expression status of p53, Fas and bcl-2 in the development of retinal degeneration in C3H, rd and rds mice. METHODS: Expression of p53, Fas and bcl-2 in the retina of rd, rds and C3H mice as well as in normal C3B mice in different periods was examined by immunohistochemical technique. RESULTS: The expression of Fas and p53 was not detected in normal C3B mice. Fas and p53 were expressed in the ganglionic layer in the early stage and then in the inner nuclear layer, while the scale and intense increased in the ganglionic layer. There is no difference of bcl-2 expression between the normal mice and mice with retinal degeneration. CONCLUSION: Fas and p53 may be involved in the retinal cell death in rd, rds, and C3B, but bcl-2 may not. It is unknown why p53 and Fas appeared first in ganglion layer but not in the outer nuclear layer where retinal cells death was noticed in early stage.

关键词：视膜 P53 Bcl Fas 表达视网膜光

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Increased exchange rate of histone H1 on chromatin by exogenous myo-genin expression

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Cell Research 2002年第Z2期 395-400页

作者： MING GONG, Ju HUA NI, HONG TI JIADepartment of Biochemistry and Molecular biology, Peking University Health Science center, Beijing 100083, China

To explore the molecular mechanism of chromatin remodeling involved in the regulation of transcriptional activation of specific genes by a myogenic regulatory factor Myogenin, we used NIH3T3 fibroblasts with a stably integrated Hl.l-GFP fusion protein to monitor histone HI movement directly by fluorescence recovery after photobleaching （FRAP） in living cells. The observation from FRAP experiments with myogenin transfected fibroblasts showed that the exchange rate of histone HI in chromatin was obviously increased, indicating that forced expression of exogenous Myogenin can induce chromatin remodeling. The hyper-acetylation of histones H3 and H4 from myogenin transfected fibroblasts was detected by triton-acid-urea （TAU）/SDS （2-D） electrophoresis and Western blot with specific antibodies against acetylated N-termini of histones H3 and H4. RT-PCR analysis indicated that the nAChR β-subunit gene was expressed in the transfected fibroblasts. These results suggest that the expression of exogenous Myogenin ca

关键词： myogenin chromatin remodeling histone acetylation histone HI mobility.

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Screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp

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Cell Research 2002年第2期12卷 133-142页

作者： YAO HUA SHI, JUN LIU, JIAN HONG XIA, JIAN FANG GUIState Key laboratory of Freshwater Ecology and Biotechnology, Wuhan center for Developmental biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 1State Key laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 1State Key laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 1State Key laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China 1State Key laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

A systemic study was initiated to identify stage-specific expression genes in fish embryogenesis by using suppression subtractive hybridization (SSH) technique. In this study, we presented a preliminary result on screen for stage-specific expression genes between tail bud stage (TBS) and heartbeat beginning stage (HBS) in gynogenetic silver crucian carp (Carassius auratus gibelio). Two SSH plasmid libraries specific for TBS embryos and HBS embryos were constructed, and stage-specific expression genes were screened between the two stages. 1963 TBS positive clones and 2466 HBS positive clones were sampled to PCR amplification, and 1373 TBS and 1809 HBS PCR positive clones were selected to carry out dot blots. 169 TBS dot blot positive clones and 272 HBS dot blot positive clones were sequenced. Searching GenBank by using these nucleotide sequences indicated that most of the TBS dot blot positive clones could not be found homologous sequences in the database, while known genes were mainly detected from HBS d

关键词： SSH embryonic development differential expression genes gynogenesis silver crucian carp.

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MicroRNA miR-24 Inhibits Erythropoiesis through Targeting Activin Type Ⅰ Receptor ALK4

MicroRNA miR-24 Inhibits Erythropoiesis through Targeting Ac...

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中国细胞生物学学会第九次会员代表大会暨青年学术大会

作者： Qiang Wang~1 Zheng Huang~2 Xue Hui-Ling~2 Xiu-Li Ju~3 Jing-Dong J.Han~2 Ye-Guang Chen~1 1 State Key Laboratory of Biomembrane and Membrane Biotechnology,Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China 2 CAS Key Laboratory of Molecular Developmental biology and center for Molecular systems biology,Institute of Genetics and Developmental biology,the Chinese Academy of Sciences,Beijing 100101,China 3 Department of Pediatrics,Qilu Hospital of Shandong University,Jinan 250012,China

MicroRNAs have been suggested to modulate a variety of cellular *** we report that miR-24 regulates erythroid differentiation by influencing the expression of human activin type I receptor ALK4 (hALK4).Ectopic express... 详细信息

关键词： microRNA miR-24 activin ALK4 erythopoiesis

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