Increased exchange rate of histone H1 on chromatin by exogenous myo-genin expression

作     者：MING GONG, Ju HUA NI, HONG TI JIADepartment of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China MING GONG, Ju HUA NI, HONG TI JIADepartment of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2002年第Z2期

页      面：395-400页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：myogenin chromatin remodeling histone acetylation histone HI mobility. 

摘      要：To explore the molecular mechanism of chromatin remodeling involved in the regulation of transcriptional activation of specific genes by a myogenic regulatory factor Myogenin, we used NIH3T3 fibroblasts with a stably integrated Hl.l-GFP fusion protein to monitor histone HI movement directly by fluorescence recovery after photobleaching （FRAP） in living cells. The observation from FRAP experiments with myogenin transfected fibroblasts showed that the exchange rate of histone HI in chromatin was obviously increased, indicating that forced expression of exogenous Myogenin can induce chromatin remodeling. The hyper-acetylation of histones H3 and H4 from myogenin transfected fibroblasts was detected by triton-acid-urea （TAU）/SDS （2-D） electrophoresis and Western blot with specific antibodies against acetylated N-termini of histones H3 and H4. RT-PCR analysis indicated that the nAChR β-subunit gene was expressed in the transfected fibroblasts. These results suggest that the expression of exogenous Myogenin ca