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^7Be content and its seasonal variation in the ground air around hangzhou area

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Nuclear Science and Techniques 1999年第4期10卷 230-234页

作者： JIANG Rangrong(zhejiang province Environmental Radiation Monitoring center, hangzhou 310012) zhejiang province Environmental Radiation Monitoring center Hangzhou 310012 China

Twice investigations around hangzhou area show that ^7Be content average in the ground air is 5.9mBq.m^-*** content of ^7Be is the highest in autumn-winter period reaching about 7.7mBq.m^-3,the next is in spring about 6.2mBq.m^-*** is in the early summer about 5.7mBq.m^-3,close to the annual average level;and the lowest in a year is in summer-autumm period with a value about 3.8mBq.m^-*** is a decreasing trend from autumn-winter period to summer-autumn of the next year,which is negatively correlated with the variation of the seasonal rainfall in hangzhou *** this trend is different from that reported by UNSCEAR:it is the highest in spring and the lowest in the late autumn,which is based only on ^7Be falling down from the ***,the present investigation shows that the seasonal rainfall is the main factor influencing the variation tend of ^7Be content in the air.

关键词： ^7Be hangzhou域浓度

Cloning of cytochrome P-450 2C9 cDNA from human liver and its expression in CHL cells

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World Journal of Gastroenterology 2002年第2期8卷 318-322页

作者： Ge-Jian Zhu Ying-Nian Yu,Department of Pathophysiology and Laboratory of Medical Molecular Biology,zhejiang University School of Medicine,hangzhou 310031,zhejiang province,china Xin Li,Department of pharmaceutical analysis & drug metabolism,College of Pharmacology Science,zhejiang University,hangzhou 310031,zhejiang province,china Yu-Li Qian, Present address:center of laboratory,Women’s hospital,School of Medicine,zhejiang University,hangzhou 310031,zhejiang province,china Department of Pathophysiology and Laboratory of Medical Molecular Biology Zhejiang University School of Medicine Hangzhou 310031 Zhejiang Province China.

AIM: Using bacterial, yeast, or mammalian cell expressing a human drug metabolism enzyme would seem good way to study drug metabolism-related problems. Human cytochrome P-450 2C9(CYP2C9) is a polymorphic enzyme responsible for the metabolism of a large number of clinically important drugs. It ranks among the most important drug metabolizing enzymes in humans. In order to provide a sufficient amount of the enzyme for drug metabolic research, the CYP2C9 cDNA was cloned and expressed stably in CHL cells. METHODS: After extraction of total RNA from human liver tissue, the human CYP2C9 cDNA was amplified with reverse transcription-polymerase chain reaction (RT-PCR), and cloned into cloning vector pGEM-T. The cDNA fragment was identified by DNA sequencing and subcloned into a mammalian expression vector pREP9. A transgenic cell line was established by transfecting the recombinant vector of pREP9-CYP2C9 into CHL cells. The enzyme activity of CYP2C9 catalyzing oxidation of tolbutamide to hydroxy tolbutamide in S9 fraction of the cell was determined by high performance liquid chromatography(HPLC). RESULTS: The amino acid sequence predicted from the cDNA segment was identical to that of CYP2C9*1, the wild type CYP2C9. However, there were two base differences, i.e. 21T>C, 1146C>T, but the encoding amino acid sequence was the same, L7, P382. The S9 fraction of the established cell line metabolizes tolbutamide to hydroxy tolbutamide; tolbutamide hydroxylase activity was found to be 0.465 +/- 0.109 ***(-1).g(-1) S9 protein or 8.62 +/- ***(-1).mol(-1) CYP, but was undetectable in parental CHL cell. CONCLUSION: The cDNA of human CYP2C9 was successfully cloned and a cell line of CHL- CYP2C9, efficiently expressing the protein of CYP2C9, was established.

关键词： Cloning, Molecular Animals Aryl Hydrocarbon Hydroxylases Cell Fractionation Cell Line china Gene Expression Humans Hypoglycemic Agents Liver Protein Isoforms Recombinant Proteins Research Support, Non-U.S. Gov't Tolbutamide

High frequencies of HGV and TTV infections in blood donors in hangzhou

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World Journal of Gastroenterology 2001年第5期7卷 637-641页

作者： Jie Yan~1 Li-Li Chen~2 Yi-Hui Luo~1 Ya-Fei Mao~1 Meng He~1 1 Department of Pathogenic Biology,Medical School of zhejiang University,hangzhou 310006,zhejiang province,china2 Department of Stomatology,The Second Affiliated Hospital,Medical School of zhejiang University,hangzhou 310009,zhejiang province,china Department of Pathogenic Biology Medical School of Zhejiang University 353 Yan An Road Hangzhou 310006 Zhejiang ProvinceChina. yanchen@***

AIM: To determine the frequencies of HGV and TTV infections in blood donors in hangzhou. METHODS: RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed. RESULTS: Thirty-two (15.8%) and 30 (14.8%) of the 203 serum samples were positive for HGV RNA and TTV DNA, respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA. Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3 serum samples compared with the reported HGV and TTV sequences was 89.36%, 87.94%, 88.65% and 63.51%, 65.77% and 67.12%, respectively. CONCLUSION: The infection rates of HGV and/or TTV in blood donors are relatively high, and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas.

关键词： Base Sequence blood Donors numerical data blood Transfusion china DNA, Viral GB virus C purification Hepatitis, Viral, Human Humans Molecular Sequence Data Polymerase Chain Reaction RNA, Viral Sequence Analysis, DNA Torque teno virus

Studies on Rapid Quantitative Analysis of Bt Toxin by Using Envirologix Kits in Transgenic Rice

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Agricultural Sciences in china 2002年第2期1卷 121-124页

作者： XIE Xiao-bo and SHU Qing-yao( Key Laboratory of Nuclear Agricultural Sciences , Ministry of Agriculture and zhejiang province ,Institute of Nuclear Agricultural Sciences , zhejiang University, hangzhou 310029 National Rice Improvement center ,china National Rice Research Institute, hangzhou 310006) Key Laboratory of Nuclear Agricultural Sciences Ministry of Agriculture and Zhejiang Province Institute of Nuclear Agricultural Sciences Zhejiang University Hangzhou 310029 National Rice Improvement center China National Rice Research Institute Hang Key Laboratory of Nuclear Agricultural Sciences Ministry of Agriculture and Zhejiang Province Institute of Nuclear Agricultural Sciences Zhejiang University Hangzhou 310029

Investigations were done on the usefulness of Envirologix CrylAb/CrylAc Plate kits for quantitative analysis of Bt toxin content in transgenic rice grains. Two transgenic rice lines: Kemingdao 1 (KMD1) and Kemingdao 2 (KMD2), transformed with a crylAb gene, and their parental variety, cv. Xiushui 11, were used as positive and negative samples. Results showed that the correlation coefficients as high as 0. 985 - 0. 998, significant at probability level of 0. 05 or 0.01, were obtained for linear regression equations by using the appended calibrators of the kits. No significant differences were detected for values of same rice sample obtained from different trials or by using different lots of the product (kit). The detectable Bt toxin content by this method could be as low as 0.5 ng/g. The Envirologix Kit could be useful for rapid quantitative detection of Bt toxin in rice grains because of its preciseness, simplicity and time saving.

关键词： Envirologix CrylAb/CrylAc plate kit Bt toxin Transgenic Rice

Complete hypersurfaces in a 4-dimensional hyperbolic space

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Applied Mathematics(A Journal of Chinese Universities) 2009年第3期24卷 370-378页

作者： XU Hong-wei1 ZHAO En-tao2 center of Mathematical Sciences,zhejiang University,hangzhou 310027,china center of Mathematical Sciences Zhejiang University Hangzhou China

This paper gives a classification of complete hypersurfaces with nonzero constant mean curvature and constant quasi-Gauss-Kronecker curvature in the hyperbolic space H4（-1）,whose scalar curvature is bounded from below.

关键词： complete hypersurface mean curvature quasi-Gauss-Kronecker curvature

Nonlinear properties of fractal growth of thin films at low temperature

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Communications in Nonlinear Science and Numerical Simulation 2001年第2期6卷 61-67页

作者： Fengmin WU, Jiefang ZHANG, Gen BIAN and Ziqin WU Research center of Science, zhejiang University of Technology, hangzhou 310014, china Institute of Nonlinear Physics, zhejiang Normal University, Jinhua 321004, china Shao Yifu Hospital of zhejiang Provin Research center of Science Zhejiang University of Technology Hangzhou 310014 China Institute of Nonlinear Physics Zhejiang Normal University Jinhua 321004 China Shao Yifu Hospital of zhejiang province Hangzhou 310005 China Department of Astronomy and Applied Physics University of Science and Technology of China Hefei 230026 China

Fractal growth of thin films at low temperature (75-200 K) is simulated by kinetic Monte Carlo method, using realistic growth model-Ag on Pt(111) surface and physical parameters. It is shown that the fractal growth of thin films is nonlinear, i. e., the island size S(t) grows with time t as tk ) where the growth exponent k is slightly less than 1 because of the growth competition of islands and the competition between nucleation and growth of islands. The further results indicate that the branch width of islands b increases with increasing island size S as b -Sa, where a is approximately equal to 1/3 because of the geometrical structure of the triangular substrate.

关键词： fractal growth thin film Monte Carlo method

人类白细胞抗原基因分型方法分辨水平的专家共识

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中国输血杂志 2024年第7期37卷 721-727页

作者：中国血小板基因数据库协作组浙江省血液中心和艳敏毛伟李晓丰沈捷刘铮朱发明不详浙江省血液中心重庆市血液中心辽宁省血液中心江苏省人民医院河南省红十字血液中心

人类白细胞抗原(human leukocyte antigen,HLA)基因分型已广泛应用于造血干细胞捐献者资料库建立、供受者组织配型、血小板基因数据库建立以及疾病关联诊断、遗传学和其他科学研究。随着HLA等位基因数量的增加和分型技术发展,对于HLA基... 详细信息

人类白细胞抗原(human leukocyte antigen,HLA)基因分型已广泛应用于造血干细胞捐献者资料库建立、供受者组织配型、血小板基因数据库建立以及疾病关联诊断、遗传学和其他科学研究。随着HLA等位基因数量的增加和分型技术发展,对于HLA基因分型分辨水平界定、供受者HLA位点结果描述和配合判定等存在一定差异,为提高对HLA基因分型分辨水平的界定和解读的规范性,HLA基因检测实验室和临床移植领域的多名专家,依据国内外相关文献和临床实践制定此专家共识,对国内HLA基因分型分辨水平的定义、分型方法、供受者结果描述和相合判定等进行了概述并达成共识,以期进一步规范实验室HLA基因分型,保障检测结果的准确性。

关键词：人类白细胞抗原等位基因基因分型方法分辨水平描述方式

Effect of surface passivation on optical and electronic properties of ultrathin silicon nanosheets

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Science china(Information Sciences) 2012年第6期55卷 1469-1474页

作者： WANG Le 1,2,WU Ke 2,DONG QianMin 1,LI XiaoYan 1,XIONG SiYu 1,XU LeiTing 3 & LIANG Pei 1 1 College Of Electronic Science and Engineering,china Jiliang University,hangzhou 310018,china 2 Department of engineering,zhejiang University,hangzhou 310000,china 3 Hang Zhou Urban Infrastructure Construction and Development center,hangzhou 310000,china College Of Electronic Science and Engineering China Jiliang University Hangzhou China Department of engineering Zhejiang University Hangzhou China Hang Zhou Urban Infrastructure Construction and Development center Hangzhou China

The binding energy,electronic structure and optical properties of H-,H 3 C-,and HS-cluster-passivated ultrathin silicon (single-layer and double-layer) nanosheets were calculated using density functional theory based on the plane-wave ultra-soft ***,the most stable configuration was selected from passivated configurations according to the principle of lowest energy after calculating their total *** the density of state and the band structure of the different passivated systems were *** was found that different passivation clusters could affect the forbidden band,theremore the passivated cluster with sulfur could greatly decrease the width of the forbidden band through electron ***,the light absorption and reflection properties were also *** results were conducive to the development of silicon-based optoelectronic devices.

关键词： density functional theory surface passivation super-thin silicon nanosheets electronic structure

ON THE CONVERGENCE OF KING-WERNER ITERATIONMETHOD IN BANACH SPACEON THE CONVERGENCE OF KING-WERNER ITERATIONMETHOD IN BANACH SPACE

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Journal of Computational Mathematics 2000年第5期18卷 457-466页

作者： Zheng-da Huang (Department of Mathematics, XiXi Campus, zhejiang University, hangzhou 310028, china) Department of Mathematics XiXi Campus Zhejiang University Hangzhou 310028 China

In this paper. a Kantorovitch- Ostrowski type convergence theorem and an error estimate of using the information of higher derivatives at the center between initial points for King-Werner iteration method in Banach ... 详细信息

关键词： Information at the center between initial points King-Werner iteration method Convergence Error estimate.