High frequencies of HGV and TTV infections in blood donors in Hangzhou

作     者：Jie Yan~1 Li-Li Chen~2 Yi-Hui Luo~1 Ya-Fei Mao~1 Meng He~1 1 Department of Pathogenic Biology,Medical School of Zhejiang University,Hangzhou 310006,Zhejiang Province,China2 Department of Stomatology,The Second Affiliated Hospital,Medical School of Zhejiang University,Hangzhou 310009,Zhejiang Province,China 

作者机构：Department of Pathogenic Biology Medical School of Zhejiang University 353 Yan An Road Hangzhou 310006 Zhejiang ProvinceChina. yanchen@*** 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2001年第7卷第5期

页      面：637-641页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

主　　题：Base Sequence Blood Donors numerical data Blood Transfusion China DNA, Viral GB virus C purification Hepatitis, Viral, Human Humans Molecular Sequence Data Polymerase Chain Reaction RNA, Viral Sequence Analysis, DNA Torque teno virus 

摘      要：AIM: To determine the frequencies of HGV and TTV infections in blood donors in Hangzhou. METHODS: RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed. RESULTS: Thirty-two (15.8%) and 30 (14.8%) of the 203 serum samples were positive for HGV RNA and TTV DNA, respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA. Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3 serum samples compared with the reported HGV and TTV sequences was 89.36%, 87.94%, 88.65% and 63.51%, 65.77% and 67.12%, respectively. CONCLUSION: The infection rates of HGV and/or TTV in blood donors are relatively high, and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas.