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Sequence analysis of mRNA polyadenylation signals of rice genes

Chinese Science Bulletin 2006年第9期51卷 1069-1077页

作者： LU Ying1, 2, GAO Chenxi1,2,3 & HAN Bin1,3,41. National Center for Gene research, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3. institute of Plant Physiology and Ecology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China National Center for Gene research Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200233 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The formation of eukaryotic mRNAs involves the cleavage and polyadenylation of pre- mRNAs. To investigate the sequence requirement of putative polyadenylation signals (PASs), poly(A) sites and downstream elements (DUEs) in 3′-end-pro- cessing in rice, we compared expressed sequences tags (ESTs) with poly(A) extremity to full-length cDNA sequences and constructed a database of 12969 pre- mRNA sequences in ?40―+40 nt surrounding the poly(A) sites, which were from 9953 genes. The al- ternative poly(A) sites were revealed in approxi- mately 25% of mRNAs. Nearly 80% of pre-mRNAs showed stringent requirement of the YA (CA or UA) at poly (A) sites for polyadenylation. About 7.9% had the AAUAAA signals on ?40―?1 nt upstream of the poly(A) sites. Over 60% of mRNAs probably used the one- or two-base variants of AAUAAA hexamers as their PASs in 3′ fragments. The single-base variants of AAUGAA revealed the high frequency in 11.5% of 3′ fragments. The DUEs were detected in 90% of pre- mRNAs, especially more than half of the pre-mRNAs with multi-base variants of AAUAAA had the DUEs surrounding the poly(A) site. The location of DUE is also important for defining the cleavage site. Al- though most of the rice pre-mRNAs did not contain AAUAAA signal, the existence of downstream ele- ments ensured the efficiency of cleavage-polyade-

关键词：多聚腺苷酸序列分析 mRNA 水稻基因工程

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THE　ROLE　PLAYED　BY　WASH　LOAD　DEPOSITION　IN　THE　FORMATION　OF　DIFFERENT　RIVER　PATTERNS

引用

international Journal of Sediment research 1994年第3期 156-165页

作者： Zhaohui Wan Jinn Gao( institute of Water Conservancy & Hydroelectric power research, 100044P.O.box 366,Beijing,China.)

ＴＨＥＲＯＬＥＰＬＡＹＥＤＢＹＷＡＳＨＬＯＡＤＤＥＰＯＳＩＴＩＯＮＩＮＴＨＥＦＯＲＭＡＴＩＯＮＯＦＤＩＦＦＥＲＥＮＴＲＩＶＥＲＰＡＴＴＥＲＮＳＴｈｅｌｏｗｅｒｌａｙｅｒｏｆｔｈｅｂｅｄｍａｔｅｒｉａｌｃｏｎｓｉｓｔｓ... 详细信息

ＴＨＥＲＯＬＥＰＬＡＹＥＤＢＹＷＡＳＨＬＯＡＤＤＥＰＯＳＩＴＩＯＮＩＮＴＨＥＦＯＲＭＡＴＩＯＮＯＦＤＩＦＦＥＲＥＮＴＲＩＶＥＲＰＡＴＴＥＲＮＳＴｈｅｌｏｗｅｒｌａｙｅｒｏｆｔｈｅｂｅｄｍａｔｅｒｉａｌｃｏｎｓｉｓｔｓｏｆｔｉｎｅｓａｎｄａｎｄｔｈｅｕｐｐｅｒｌａｙｅｒｃｏｎｓｉｓｔｓｏｆａｍｉｘｔｕｒｅｏｆｃｌａｙ（１０％）ａｎｄｔｉｎｅｓａｎｄ（９０％）．ＮＡＴＵＲＡＬＲＩＶＥＲＳｌ．ＣｈａｎｇｅｏｆｃｈａｎｎｅｌｐａｔｔｅｒｎｓａｌｏｎｇａｒｉｖｅｒｃｏｕｒｓｅＩｔｉｓｃｏｍｍｏｎｔｈａｔｉｎｔｈｅｕｐｐｅｒＰａｒｔｏｆａｎａｌｌｕｖｉａｌｒｉｖｅｒｔｈｅｌｏｎｇｉｔｕｄｉｎａｌｓｌｏｐｅｉｓｓｔｅｅｐａｎｄｔｈｅｒｉｖｅｒｃａｒｒｉｅｓｈｅａｖｙｓｅｄｉｍｅｎｔｌｏａｄ．Ｄｕｅｔｏｔｈｅｓｔｅｅｐｓｌｏｐｅａｎｄａｓｓｏｃｉａｔｅｄｈｉｇｈｖｅｌｏｃｉｔｙｃｌａｙｓｅｔｔｌｅｓｌｉｔｔｌｅｅｖｅｎｏｎｔｌｏｏｄｐｌａｉｎ．Ｆｌｏｏｄｐｌａｉｎｏｒｒｉｖｅｒｈａｎｋｃｏｎｓｉｓｔｓｏｆｓｉｌｔａｎｄｔｉｎｅｓａｎｄ．Ｔｈｅｒｉｖｅｒｂｅｈａｖｅｓａｓａｗａｎｄｅｒｉｎｇｏｎｅ．Ｐａｓｓｉｎｇａｃｅｒｔ

关键词： River pattern wash load deposition main channel flood plain

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SHOCK　COMPRESSION　BEHAVIOR　OF　BROMOFORM

引用

原子与分子物理学报 1996年第4期 5-11页

作者： a. Lab for Shock Wave and Detonation Physics research, Southwest institute of Fluid Physics, P.O.box 523-61, Chengdu, Sichuan 610003, P.R.China b. institute of Applied Physics, Sichuan Union University, Chengdu, Sichuan 610065, P.R.China c. Current

ＳＨＯＣＫＣＯＭＰＲＥＳＳＩＯＮＢＥＨＡＶＩＯＲＯＦＢＲＯＭＯＦＯＲＭＰｅｎｇＳｈａｎｇｑｉａｎｇａ，ｂ，ｃＪｉｎｇＦｕｑｉａｎａ，ｂＨｕＪｉｎｂｉａｏａ，ｂＧｏｕＱｉｎｇｑｕａｎｂａ．ＬａｂｆｏｒＳｈｏｃｋＷａｖ... 详细信息

ＳＨＯＣＫＣＯＭＰＲＥＳＳＩＯＮＢＥＨＡＶＩＯＲＯＦＢＲＯＭＯＦＯＲＭＰｅｎｇＳｈａｎｇｑｉａｎｇａ，ｂ，ｃＪｉｎｇＦｕｑｉａｎａ，ｂＨｕＪｉｎｂｉａｏａ，ｂＧｏｕＱｉｎｇｑｕａｎｂａ．ＬａｂｆｏｒＳｈｏｃｋＷａｖｅａｎｄＤｅｔｏｎａｔｉｏｎＰｈｙ...

关键词： Bromoform Shock compression EOS Two stage light gas gun

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库车前陆褶级冲断带的互嵌构造样式

引用

石油地球物理勘探 1999年第3期34卷 302-308页

作者：胡学平石油地球物理勘探局第二地质调查处

胡学平．库车前陆褶皱冲断带的互嵌构造样式．石油地球物理勘探，1999，34（3）：302～308在对经过露头资料和钻井资料标定的地震剖面解释之后，发现在库车前陆诏皱冲断带库姆格列木背斜下面存在一种特殊的断层结构，致使新老地层互相... 详细信息

胡学平．库车前陆褶皱冲断带的互嵌构造样式．石油地球物理勘探，1999，34（3）：302～308在对经过露头资料和钻井资料标定的地震剖面解释之后，发现在库车前陆诏皱冲断带库姆格列木背斜下面存在一种特殊的断层结构，致使新老地层互相嵌入，形成互嵌构造。本文依据橹皱冲断带的构造理论及盐岩的流变机制，并运用平衡剖面的观点，对互嵌构造的形成机制进行了探讨，同时给出了与互嵌构造有关的断层平面组合方法。

关键词：前陆盆地褶皱冲断带互嵌构造断层油气勘探

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Preparation of Silicon Carbide with High Properties

引用

Journal of Materials Science & Technology 2001年第1期17卷 53-54页

作者： Guangiiang YUAN Yang ZHOU and Daming CHEN National Key Laboratory of Advanced Composites, Beijing institute of Aeronautical Materials, P.O. box 81-3, Beijing 100095, China Bangsheng LI School of Materials Science and Engineering, Harbin institute of Tech Beijing Inst Aeronaut Mat Natl Key Lab Adv Composites Beijing 100095 Peoples R China Harbin Inst Technol Sch Mat Sci & Engn Harbin 150001 Peoples R China

In order to prepare silicon carbide with high properties, three kinds of SiC powders A, B, and C with different composition and two kinds of additives, which were Y2O3-A12O3 system and Y2O3-La2O3 system, were used in this experiment. The properties of hot-pressed SiC ceramics were measured. With the same additives, different SiC powder resulted in different properties. On the other hand, with the same SiC powder, increasing the amount of the additive Y2O3Al2O3 improved properties of SiC ceramics at room temperature, and increasing the amount of the additive Y2O3-La2O3 improved property SiC ceramics at elevated temperature. In addition. the microstructure of SiC ceramics was studied by scanning electron microscopy.

关键词： SiC Preparation of Silicon Carbide with High Properties

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Genetic Identification of a New Small Grain Dwarf Gene in rice

引用

rice Science 2003年第Z1期 11-15页

作者： WU Cheng~1, LI Xiu-lan~2, DENG Xiao-jian~(1,2), WANG Ping-rong~2, LI Ren-duan~2, YANG Zhi-rong~1 (~1 College of Life Science, Sichuan University, Chengdu 610064, China ~2 rice research institute, Sichuan Agricultural University, Chengdu 611130, China)

The plant material used in the study was rice line 162d, a new small grain dwarf mutant. Polymorphic analysis of 221 SSR loci demonstrated that 162d derived from a semidwarf variety Shuhui 162 through mutation, and 162d and Shuhui 162 were just a pair of near isogenic lines. Genetic analysis of F1and F2populations suggested that dwarfism in 162d was controlled by a single recessive gene. Phenotypic characteristics of the mutant gene were that plant height was about a quarter of normal height, grain size about a quarter of normal size, leaf was short and broad, and seed setting rate was very low, compared with the near isogenic line Shuhui 162. The mutant gene was sensitive to gibberellin （GA3） treatment and did not located on the region near the centromere of rice chromosome 5, where dl gene located. Therefore, it was concluded that the mutant gene of 162d was a new small grain dwarf gene in rice.

关键词： rice dwarf gene genetic identification genetic marker gene location near isogenic line

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Corn　Growth　as　Affected　by　Plastic　Cover　Under　Drip　Irrigation　Condition　in　the　Desert

引用

Pedosphere 1996年第3期 243-249页

作者： WANG XUE-FENG, XU FU-AN and U. SHANI(institute of Soil Science. Academia Sinica. P.O.box 821. Nanjing 210008 China)(Arava research Station. Eilot 88820 Israel)

ＣｏｒｎＧｒｏｗｔｈａｓＡｆｆｅｃｔｅｄｂｙＰｌａｓｔｉｃＣｏｖｅｒＵｎｄｅｒＤｒｉｐＩｒｒｉｇａｔｉｏｎＣｏｎｄｉｔｉｏｎｉｎｔｈｅＤｅｓｅｒｔ￥ＷＡＮＧＸＵＥ－ＦＥＮＧ，ＸＵＦＵ－ＡＮａｎｄＵ．ＳＨＡＮＩ（Ｉｎｓ... 详细信息

ＣｏｒｎＧｒｏｗｔｈａｓＡｆｆｅｃｔｅｄｂｙＰｌａｓｔｉｃＣｏｖｅｒＵｎｄｅｒＤｒｉｐＩｒｒｉｇａｔｉｏｎＣｏｎｄｉｔｉｏｎｉｎｔｈｅＤｅｓｅｒｔ￥ＷＡＮＧＸＵＥ－ＦＥＮＧ，ＸＵＦＵ－ＡＮａｎｄＵ．ＳＨＡＮＩ（ＩｎｓｔｉｔｕｔｅｏｆＳｏｉｌＳｃｉｅｎ...

关键词： corn growth. drip irrigation plastic cover water use efficiency

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己烷低耗喷雾热解法大规模制备多壁碳纳米管(英文)

引用

新型炭材料 2009年第1期24卷 33-38页

作者： Z. Sadeghian Del)artment of Gas Research Institute of Petroleum Industry ( RIPI) P. O. Box 14857-3311 West Blvd. Azadi sport complex Tehran Iran

以己烷为碳源,二茂铁为催化剂前躯体,应用喷雾热解法,制备了多壁碳纳米管(MWCNTs)。采用酸沥滤和空气氧化对MWCNTs进行纯化。利用SEM、TEM、XRD、EDS、TGA及Raman光谱分析等方法分别对原生和纯化MWCNTs进行表征。为制得优质、高产MWCNTs... 详细信息

以己烷为碳源,二茂铁为催化剂前躯体,应用喷雾热解法,制备了多壁碳纳米管(MWCNTs)。采用酸沥滤和空气氧化对MWCNTs进行纯化。利用SEM、TEM、XRD、EDS、TGA及Raman光谱分析等方法分别对原生和纯化MWCNTs进行表征。为制得优质、高产MWCNTs,对制备工艺参数作了优选,分别研讨了最佳制备参数,包括:二茂铁升华温度、己烷中二茂铁浓度、热解温度和时间,己烷与H2的流量比。MWCNTs具有典型的腊肠状(Sawsage-like)构型,长度大于数十微米,内、外管径分别为15nm～45nm及25nm～70nm,MWCNTs的纯度和产率的质量分数分别高于95%和70%。

关键词：己烷喷雾热解大规模制备多壁碳纳米管

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Cloning and expression analysis of cold induced gene oscr from Chaling wild rice

Cloning and expression analysis of cold induced gene oscr fr...

引用

中国作物学会50周年庆祝会暨2011年学术年会

作者： Rongjun Chen rice research institute of Sichuan Agricultural University,Chengdu,Sichuan 611134,China

Objective The objective of this project is to characterize genes with important roles in cold stress tolerance and to understand how they function. Abstract Cold is one of common environmental stress factors that influences plant growth and development;and is one of the major limits on rice *** is important to determine and understand functions of critical genes involved in cold-tolerance in rice for trait improvement by molecular breeding or genetic *** this purpose we analyzed the global gene expression profiles of cold-tolerant Chaling wild rice and cold-sensitive indica rice p64s using Affymetrix array *** found a cold-induced gene,oscr,which is only expressed in cold-stressed cl wild rice,but not in *** promoter regions and cDNAs of the oscr gene alleles from both types of rice were *** sequences of the promoter regions are different between the cold-tolerant rice and cold-sensitive *** is a 450 bp insert in the promoter region of the cold-sensitive rice that is absent in cold-tolerant *** designed PCR primers that differentiate the two different types of rice among 182 *** expression pattern of oscr was analyzed in cold-tolerant wild rice,japonica rice,cold-sensitive indica rice and the F 1 and F2 progenies of Chaling wild rice x 9311. Conclusion A cold-induced gene,oscr,was cloned from cold-tolerant wild *** oscr gene is only expressed in cold-tolerant rice under cold *** analyses indicate that the 450 bp insert in the promoter region of the cold-sensitive rice leads to no expression under normal or cold *** have constructed an expression vector for the cold-tolerant allele of the oscr gene and are currently transforming it into the cold-sensitive rice to test its effect.

关键词：

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Expression analysis of gdcsP promoter from C_3-C_4 intermediate plant Flaveria anomala in transgenic rice

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Chinese Science Bulletin 2001年第19期46卷 1635-1638,1675页

作者： CHEN Shuai, QU Nan, CAO Shouyun, Hermann Bauwe. CHEN Shouyi, TIAN Wenzhong & CHU Chengcai1. institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China 2. institute of Plant Genetics and Crop Plant research (IPK), 06466 Gatersleben, Germany 3. Rostock University, 18055 Rostock, Germany institute of Genetics Chinese Academy of Sciences Beijing China institute of Plant Genetics and Crop Plant research (IPK) Gatersleben Germany Rostock University Rostock Germany

The gdcsP promoter isolated from C3-C4 intermediate plant Flaveria anomala was fused to the β-glu-curonidase (GUS) gene. The chimeric gene was inserted into the binary vector pBin19 and introduced into the rice (Oryza saliva L.) cv. 8706 by Agrobacterium-mediated gene transfer. GUS activity can be detected in leaf, leaf sheath, stem and root tissues via fluorometric GUS assay. However, no GUS activity was found in mature endosperm. Histochemical localization revealed that GUS expression was exclusively restricted to vascular tissues in transgenic plants. This promoter also showed spatial-temporal expression patterns that GUS expression declined significantly with the maturity of plants. These expression patterns make the gdcsP promoter extremely valuable in the applied biotechnology that needs target gene expression restricted to vascular tissues.

关键词： rice (Oryza sativa L.) glycine decarboxyla.se P-protein subunit vascular tissue.

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