Cloning and expression analysis of cold induced gene oscr from Chaling wild rice
会议名称：《中国作物学会50周年庆祝会暨2011年学术年会》
会议日期：2011年
摘 要：Objective The objective of this project is to characterize genes with important roles in cold stress tolerance and to understand how they function. Abstract Cold is one of common environmental stress factors that influences plant growth and development;and is one of the major limits on rice *** is important to determine and understand functions of critical genes involved in cold-tolerance in rice for trait improvement by molecular breeding or genetic *** this purpose we analyzed the global gene expression profiles of cold-tolerant Chaling wild rice and cold-sensitive indica rice p64s using Affymetrix array *** found a cold-induced gene,oscr,which is only expressed in cold-stressed cl wild rice,but not in *** promoter regions and cDNAs of the oscr gene alleles from both types of rice were *** sequences of the promoter regions are different between the cold-tolerant rice and cold-sensitive *** is a 450 bp insert in the promoter region of the cold-sensitive rice that is absent in cold-tolerant *** designed PCR primers that differentiate the two different types of rice among 182 *** expression pattern of oscr was analyzed in cold-tolerant wild rice,japonica rice,cold-sensitive indica rice and the F 1 and F2 progenies of Chaling wild rice x 9311. Conclusion A cold-induced gene,oscr,was cloned from cold-tolerant wild *** oscr gene is only expressed in cold-tolerant rice under cold *** analyses indicate that the 450 bp insert in the promoter region of the cold-sensitive rice leads to no expression under normal or cold *** have constructed an expression vector for the cold-tolerant allele of the oscr gene and are currently transforming it into the cold-sensitive rice to test its effect.