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Influence of mild hypothermia on vascular endothelial growth factor and infarct volume in brain tissues after cerebral ischemia in rats

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Neural Regeneration Research 2006年第9期1卷 847-849页

作者： Fei Ye1, Gangming Xi1, Biyong Qin1, Shifeng Wang1, Chengyan Li2 1department of neurology, People’s hospital of Yunyang Medical College, Shiyan 442000, hubei province, china 2department of neurology, renmin hospital of wuhan university, wuhan 430060, hubei province, china department of neurology People's Hospital Yunyang Medical College Shiyan 442000 Hubei Province China department of neurology Renmin Hospital Wuhan University Wuhan 430060 Hubei Province China

BACKGROUND: It has been demonstrated that mild hypothermia has obvious protective effect on both whole and local cerebral ischemia. However, the definite mechanism is still unclear for the brain protection of mild hypothermia on cerebral edema, inhibiting inflammatory reaction, stabilizing blood brain barrier, etc. OBJECTIVE: To investigate the effect of mild hypothermia on the expression of vascular endothelial growth factor and the infarct volume after cerebral ischemia in rats, and analyze the brain protective mechanism of mild hypothermia. DESIGN: A randomized grouping and controlled animal trial. SETTING: department of neurology, People’s hospital of Yunyang Medical College. MATERIALS: Twenty adult male SD rats of clean degree, weighing (250±30) g, were provided by the animal experimental center, School of Medicine, wuhan university. The kits for SP immunohistochemistry were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. METHODS: The experiments were carried out in the laboratory of department of neurology, Renmen hospital of wuhan university from May to July 2005. ① The 20 rats were divided randomly into normal temperature group (n =10) and mild hypothermia group (n =10). Models of permanent middle cerebral artery occlusion were established with modified nylon suture embolization. The rats were assessed with the Longa standards: 0 point for without nerve dysfunction; 1 for mild neurological deficit (fore claws could no extend completely); 2 for moderate neurological deficit (circling towards the affected side); 3 for severe neurological deficit (tilting towards the affected side); 4 for coma and unconscious; 1-3 points represented that models were successfully established. The rats of the normal temperature group were fed at room temperature, and those in the mild hypothermia group were induced by hypothermia from 2 hours postoperatively, and the rectal temperature was kept at 34-35 ℃ for 72 hours. ② Measurement of infarct volume: All t

关键词： infarct neurological purchased hemisphere protective minutes Biotechnology stained occlusion wuhan

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Embryo-chondrocyte expressed gene 1,downregulating hypoxiainducible factor 1α,is another marker of lung tumor hypoxia

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Acta Pharmacologica Sinica 2007年第4期28卷 549-558页

作者： Li ZHANG Qing-quan LI department of Respiratory Disease,renmin hospital,wuhan university,wuhan 430060,china department of Respiratory Disease Renmin Hospital Wuhan University Wuhan China

Aim:To explore the mechanism of differentiated embryo-chondrocyte expressedgene 1 (DEC 1) in the lung adenocarcinoma A549 cell line and its relationship withhypoxia-inducible factor 1α(HIF-1α).Methods:DEC 1 and HIF-1α protein levelswere detected in lung adenocarcinoma tissues by immunohistochemistry.A549cells were cultured at *** assay was used to determine the effect of *** apoptotic analysis was determined by flow ***-PCR andimmunocytochemistry were carried out to observe the DEC 1 and HIF-1α mRNAand protein ***-1α mRNA and protein levels were detected in thestably transfected pcDNA-DEC 1+/-A549 cells by RT-PCR and Western ***1 mRNA and protein levels were detected in the stably transfected pcDNA-DEC 1+/-A549 cells with or without H1F-1α antisense oligonucleotide treatmentby RT-PCR and Western ***:DEC1 was specifically located in 40(48.8%) lung adenocarcinoma *** results of the MTT test showed thegrowth of the stably transfected pcDNA-DEC+ A549 cells was higher than thoseof A549 cells and the stably transfected pcDNA-DEC-A549 *** alsoinduced the apoptosis of A549 cells and the stably transfected pcDNA-DEC+/-A549 *** increased the mRNA and protein levels of DEC1 and HIF-1α.Both HIF-1α mRNA and protein levels decreased in the stably transfected pcDNA-DEC+cells,but HIF-1α antisense oligonucleotide had no effect on DEC1 in thestably transfected pcDNA-DEC+/-A549 ***:DEC 1 is a marker oftumor ***1 downregulates the HIF-1α at both mRNA and protein levelsat hypoxia in lung adenocarcinoma A549 cells.

关键词： DECI lung adenocarcinoma hypoxia HIF-1

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Clinical and endoscopic analysis of gastric Dieulafoy’s lesion

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World Journal of Gastroenterology 2010年第5期16卷 631-635页

作者： Yi-Juan Ding,Liang Zhao,Jun Liu,He-Sheng Luo,department of Gastroenterology,renmin hospital of wuhan university, Jiefang Road 238,wuhan 430060,hubei province,china department of Gastroenterology Renmin Hospital of Wuhan University Jiefang Road 238 Wuhan 430060 Hubei Province China.

AIM:To investigate the incidence,location,clinical presentation,diagnosis and effectiveness of endoscopic treatment of gastric Dieulafoy's lesion(DL)in china. METHODS:All patients who received emergency upper gastrointestinal(GI)endoscopy due to gastric DL from February 2000 to August 2008 at GI endoscopy center of renmin hospital of wuhan university were included in this *** clinical presentation,medical history,location and characteristics of DL methods and effectiveness of therapy of patients with DL were retrospectively analysed by chart ***-term follow-up data were collected at outpatient clinics or telephone interviews. RESULTS:Fifteen patients were diagnosized with DL,which account for 1.04%of the source of bleed- ing in acute non-variceal upper GI *** comorbidities were found in one patient with hypertension and diabetic *** or combined therapy with hemoclip produced primary hemostasis in 92.8%(13/14) of patients. CONCLUSION:DL is uncommon but life-threatening in *** proved to be safe and effective in controlling bleeding from DL.

关键词： Gastric Dieulafoy’s lesion Clinical analysis Endoscopic analysis

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Detection of type Ⅳ collagenase activity in malignant ascites

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World Journal of Gastroenterology 2003年第11期9卷 2592-2595页

作者： Xiao-Min Sun Wei-Guo Dong Bao-Ping Yu He-Sheng Luo Jie-Ping Yu, department of Gastroenterology, renmin hospital, wuhan university, wuhan 430060, hubei province, china department of Gastroenterology Renmin Hospital Wuhan University Wuhan 430060 Hubei Province China.

AIM: Type IV collagenase participates in invasion and metastasis of cancer cells. Malignant asdtes is a manifestation of advanced malignant disease that is associated with invasion and metastasis of the peritoneal cavity. Thus, it is reasonable to hypothesize that type IV collagenase is linked to malignant ascites. The purpose of our study was to detect type IV collagenase activity in malignant ascites so as to provide the scientific basis for clinic diagnosis and treatment of malignant ascites. METHODS: Cirrhotic ascites (n=36), tuberculous ascites (n=8) and malignant ascites (n=-23) from patients with gastric cancer (n=6), colon cancer (n=5), ovarian cancer (n=8) and other cancers (n=4), including 2 hepatocellular cancers, 1 pancreatic cancer, 1 primary peritoneal carcinoma were collected by paracentesis. The ascites were made cellfree by centrifugation and stored frozen at -70℃ before determination. Type IV collagenase activity was determined by gelatin zymography. RESULTS: The activity of matrix metalloproteinases-2 and -9 could not be detected in ascites of hepatic cirrhosis and tuberculous peritonitis but could be detected in 20 and 18 out of 23 malignant ascites respectively. The positive rate of type IV collagenase (MMP-2, 87.0% and MMP-9, 78.3%) was higher than that by routine ascites tests (P2 was higher than that of MMP-9 (P=0.022<0.05). CONCLUSION: Type IV collagenase is positive in malignant ascites. Detection of type IV collagenase activity is useful in qualitative diagnosis of ascites. Type IV collagenase may play an important role in malignant ascites formation.

关键词：恶性腹水 IV型胶原酶肿瘤转移恶性肿瘤发病机制

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Quantitative analysis of intestinal gas in patients with acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2012年第3期11卷 314-318页

作者： Ying Liu and He-Sheng Luo department of Gastroenterology, renmin hospital, wuhan university, wuhan 430060, china department of Gastroenterology Renmin Hospital Wuhan University Wuhan 430060 China

BACKGROUND: Disturbance of gastrointestinal function is a common complication in the early phase of acute pancreatitis (AP). Intestinal gas may reflect the function of the gut. Using plain abdominal radiographs, we investigated whether intestinal gas volume is related to AP. METHODS: Plain abdominal radiographs of 68 patients with AP within 24 hours after admission and 21 normal controls were digitized and transmitted to a computer. The region of intestinal gas was identified by an image manipulation software and the gas volume score (GVS) was calculated. The relationships between the GVS values and various clinical factors of AP were analyzed. RESULTS: The GVS in the AP group was 0.084±0.016, in the mild AP (MAP) group 0.070±0.005, and in the severe AP (SAP) group 0.094±0.013; all values were higher than that in the control group (P2, P<0.01) and the acute physiology and chronic health evaluation II (APACHE II) scores (r=0.801, P<0.01). In addition, the GVS in patients with secondary pancreatic and/or peripancreatic infection was 0.107±0.014, higher than that in patients without secondary infection (P<0.01). GVS was not related to gender, age, etiology or clinical outcome of AP. CONCLUSIONS: Intestinal gas volume is significantly elevated in patients with AP. It is closely related to Ranson’s and APACHE II score and secondary pancreatic and/or peripancreatic infection. GVS may be a new prognostic tool for assessing the severity of AP in the early course of the disease.

关键词： acute pancreatitis gas volume score intestinal gas gut motility bacterial overgrowth

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A Microenvironment, Rather than Chemical, Initiates the Cardiomyogenic Differentiation of Marrow Stromal Cells

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wuhan university Journal of Natural Sciences 2004年第4期9卷 513-521页

作者： LIU Wei-xin 1,2 , SONG Jian 1 , WAN Yu 1, PAN Guo-dong 1 , LIU Yu 1 , CHENG Bang-chang 2 ,CHEN Xi-chang 1 1. School of Medicine, wuhan university, wuhan 430071, hubei, china 2. renmin hospital, wuhan university, wuhan 430060, hubei, china Sch. of Medicine Wuhan Univ. Wuhan 430071 China renmin hospital Wuhan Univ. Wuhan 430060 China

To investigate the potential of cardiomyogenic differentiation of rat bone marrow stromal cells (MSCs), they were exposed to 5-azacytidine tretments (single/repeat) at varying concentrations (3, 5, 10 μmol/L) and the fates of the cells were analyzed by immunocytochemistry, Western blot and the reporter gene of enhanced cyan fluorescent protein (ECFP) under the control of ventricular myosin light chain 2 (MLC2v) promoter. MSCs were also cocultured with cardiomyocytes for periods up to 16 days, the expression of cardiac myosin heavy chain (MHC) and troponin I (Tn I) proteins was analyzed. After the induction with 5-azacytidine, neither spontaneously beating cells nor myotubes were found; MHC and Tn I proteins were also undetectable and no ECFP-positive MSCs were detected. But when cocultured with cardiomyocytes, spontaneously contracting MSCs were observed and cardiac specific proteins could be detected. The results proved that the novel effects of 5-azacytidine on the cardiomyogenic differentiation of MSCs should be questioned and a direct intercellular communication with cardiomyocytes is necessary for MSCs to differentiate into cardiomyocytes. Key words cardiomyocyte - cell differentiation - stem cell CLC number Q 813.5 - R 542.2+2 Foundation item: Supported by the Natural Science Foundation of hubei province (2002AB00150)Biography: LIU Wei-xin (1976-), male, Ph. D candidate, research direction: stem cell and cellular biology.

关键词： cardiomyocyte cell differentiation stem cell

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Effect of Nimesulide on proliferation and apoptosis of human hepatoma SMMC-7721 cells

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World Journal of Gastroenterology 2002年第3期8卷 483-487页

作者： Geng Tian Jie-Ping Yu He-Sheng Luo Bao-Ping Yu Hui Yue Jian-Ying Li Oiao Mei,Gastroenterology department,renmin hospital of wuhan university,wuhan 430060,hubei province,china Gastroenterology department. renmin hospital of wuhan university 238 Jie-fang RoadWuhan 430060Hubei ProvinceChina.

AIM: Cyclooxygenase-2 (COX-2) has been suggested to be associated with carcinogenesis. We sought to investigate the effect of the selective COX-2 inhibitor, Nimesulide on proliferation and apoptosis of SMMC-7721 human hepatoma ***: This study was carried out on the culture of hepatic carcinoma SMMC-7721 cell line. Various concentrations of Nimesulide (0, 200 micromol/L, 300 micromol/L, 400 micromol/L) were added and incubated. Cell proliferation was detected with MTT colorimetric assay, cell apoptosis by electron microscopy, flow cytometry and ***: Nimesulide could significantly inhibit SMMC-7721 cells proliferation dose-dependent and in a dependent manner compared with that of the control group. The duration lowest inhibition rate produced by Nimesulide in SMMC-7721 cells was 19.06%, the highest inhibition rate was 58.49%. After incubation with Nimesulide for 72 h, the most highest apoptosis rate and apoptosis index of SMMC-7721 cells comparing with those of the control were 21.20%+/-1.62% vs 2.24%+/-0.26% and 21.23+/-1.78 vs 2.01+/-0.23 (P2 inhibitor, Nimesulide can inhibit the proliferation of SMMC-7721 cells and increase apoptosis rate and apoptosis index of SMMC-7721 cells. The apoptosis rate and the apoptosis index are dose-dependent. Under electron microscope SMMC-7721 cells incubated with 300 micromol and 400 micromol Nimesulide show apoptotic characteristics. With the clarification of the mechanism of selective COX-2 inhibitors, These COX-2 selective inhibitors can become the choice of prevention and treatment of cancers.

关键词： Apoptosis Carcinoma, Hepatocellular control Cell Division Cyclooxygenase 2 Cyclooxygenase 2 Inhibitors Cyclooxygenase Inhibitors Humans Isoenzymes inhibitors Liver Neoplasms Membrane Proteins Prostaglandin-Endoperoxide Synthases Sulfonamides Tumor Cells, Cultured

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Comprehensive Diagnostic Value of P53, p21WAF1 and Proliferating Cell Nuclear Antigen for Lung Cancer

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wuhan university Journal of Natural Sciences 2009年第5期14卷 452-456页

作者： WU Xiaojun,DING Xuhong,HU Suping department of Respiratory Diseases,renmin hospital of wuhan university,wuhan 430060,hubei,china department of Respiratory Diseases Renmin Hospital of Wuhan University Wuhan China

The expression of P53, p21WAF1 and proliferating cell nuclear antigen （PCNA） was detected in 114 samples of lung cancer patients （with 89 cases benign lung tissue as control） and the diagnostic value of these markers was evaluated. The results show the following：（1） The positive expression rates of P53, p21WAF1 and PCNA in samples of lung cancer were 47.37%, 75.44% and 80.70%, respectively, which were significantly higher than that in the samples of benign lung diseases （ p 〈 0. 001 ）. The odds ratios were 39.15, 5.75, and 6.76, respectively. This indicates that the expression of P53, p21WAF1 and PCNA was helpful for the diagnosis of lung cancer. （2） For the diagnosis of lung cancer, the positive likelihood ratio of P53 was 21.08, which were significantly higher than that of p21WAF 1 （2.16）, PCNA （2.11） and of all the combined tests. This shows that P53 expression was the most valuable for diagnosis of lung cancer. （3） For the diagnosis of lung cancer, the negative likelihood ratio of P53/p21WAF 1/PCNA parallel test was 0.057 1, which was lower than that of other single and combined tests. This indicates that P53/p21WAF 1/PCNA parallel test has high diagnostic value for exclusion of lung cancer.

关键词： lung neoplasm diagnostic testing P53 protein p21WAF 1 proliferating cell nuclear antigen

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Biological Effect of Magnetic Field in Mice

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生物医学工程学杂志 2005年第S1期22卷 171-172页

作者： Zhao-Wei ZENG(renmin hospital of wuhan university, wuhan 430060,china) renmin hospital of wuhan university Wuhan 430060 China

Objective:To study the biological effect of magnetic field in mice ***: With a piece of permanent magnet embeded in mice bodies beside the femoral artery and vein to measure the electrophoretic velocity(um/s).Result: The magnetic field in mice bodies on the experiment group that the electrophoretic velocity is faster more than control and free group. Conclusion:The magnetic field in animal’s body can raise the neg ative electric charges on the surface of erythrocyte to improve the microcirculation, this is the biological effect of magnetic field.

关键词： Magnetic field Electrophorisis Erythrocyte Mice

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Effect of angiotensin II receptor 1 antisense oligodoexynucleotides on physiological and pathophysiological growth of cardiomyocytes

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Acta Pharmacologica Sinica 2004年第5期25卷 19-27页

作者： Ying WANG2, Jin-ming WANG2, Shu-xun Yan3, Ming-jiang LI2,4, Jian-jun LI22department of Cardiology, renmin hospital, wuhan university, wuhan 430060, china 3hubei College of Traditional Chinese Medicine, wuhan 430061,china department of Cardiology Renmin Hospital Wuhan University Wuhan 430060 hubei College of Traditional Chinese Medicine Wuhan 430061

AIM: To evaluate the role of angiotensin II receptor 1 antisense oligodexynucleotides (AT1R-AS-ODNs) on physi-ological and pathophysiological growth of cardiomyocytes from normotensive rats. METHODS: Cardiomyocyteswere transfected with AT1R-AS-ODNs (200 nmol/L) followed by treatment with or without angiotensin II (1 mol/L).In situ hybridization and Western blot were used for AT1R mRNA and protein detection, respectively. c-Jun N-terminal protein kinase (JNK) activity was characterized by immune complex kinase assay. c-Jun protein expres-sion was examined by immunocytochemistry. DNA content was detected by flow cytometric assay. Atrial natri-uretic factor (ANF) expression was identified by radioimmunoassay. RESULTS: Treatment with AT1R-AS-ODNsfor 24 h resulted in 51.2 % decrease in AT1R mRNA and 60.7 % in protein (P2 h, c-Jun protein expression was increased. By 24 h, angiotensin II caused a markedincrease both in G0/G1 and G2/M DNA content, and increased ANF expression by 1.8-fold. All these were inhibitedby AT1R-AS-ODNs pretreatment. In contrast, sense sequence was ineffective. CONCLUSION: Decrease ofAT1R expression by AS-ODNs did not interfere with normal growth, but protected cardiomyocytes from angio-tensin II-dependent pathophysiological growth.

关键词： antisense oligodeoxynucleotides angiotensin receptors angiotensin II myocardium hypertrophy mitogen-activated protein kinases

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