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Diseases and Pests of Lentinula edodes Cultivated in zhejiang Province

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食用菌学报 2006年第2期 74-79页

作者： CHEN Qiao-biao~ 1 , WU Quan-cong~ 2 , WU Ying-miao~ 1 ~ 1 qingyuan science & research center of edible fungi, qingyuan, zhejiang 323800, china ~ 2 Lishui Academy of Agricultural sciences, Lishui, zhejiang 323000, china

Eighty-four different types of pathogenic and competitive microorganisms, and 92 types of pests, were identified during an investigation of diseases and pests associated with Lentinula edodes production in zhejiang Province of china that was conducted during the period 1999～2004. Microbial types belonged to the Eumycophyta, Bacteria, Myxomycota, Actinomycetes and Viruses, and the pests to Arthropoda and Mollusca. The major diseases of L. edodes were caused by Trichoderma, Mucor, Rhizopus, Aspergillus flavus Link, Rhodotorula and Monilia. Lycoriella pleuroti Yang et Zhang and a Scatopse species were the preponderant pest species, although Mycophila fungicola Felt was responsible for causing major levels of damage.

关键词： Lentinula edodes Mushroom diseases Mushroom pests

rDNA ITS Sequence Analysis and Molecular Phylogeny of Four Fungal Strains Assigned to the Family Russulaceae

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食用菌学报 2007年第2期 29-32页

作者： FU Lizhong1, 2, LI Haibo1, 2, WEI Hailong1, 2, WU Qingqi1, 2, WU Dafeng2, WU Xueqian1, 21 Biotechnology Institute, zhejiang Academy of Forestry science, Hangzhou, zhejiang 310023,china 2Lishui edible fungi research and Development center,zhejiang Essence fungi DevelopmentCompany Ltd,Lishui,zhejiang 323000,china

Internal transcribed spacer (ITS) sequences of four edible and medicinal fungi belonging to the Russulaceae, collected from the Lishui mountainous area of zhejiang Province, were cloned and sequenced. Comparisons with sequence data in GenBank were undertaken using BLAST, and the four ITS sequences, together with 15 reference sequences obtained from GenBank, were used to construct a phylogenetic tree. rDNA ITS sequences of the four strains ranged from 673 to 766 bp in length and between 47.4% and 49.48% in GC content. Phylogenetic data indicated strain R57 to be Russula cyanoxantha. Differences in the size and GC content of ITS sequences from strains R32 and R57 (R. cyanoxantha) and strain L37 (Lactarius sanguifluus) were significant, whereas the corresponding differences between strains R32 and R57 were relatively small. A close kinship, with only small detectable differences in their respective ITS sequences, existed between R. mustelina, R. virescens and R. parazurea. A similar situation applied to strains of L. sanguifluus and L. salmonicolor.

关键词： Russulaceae rDNA Internal Transcribed Spacer Sequence analysis Phylogeny Macrofungi

Optimization of a Sequence-Related Amplified Polymorphism (SRAP) Amplification System for Lentinula edodes

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食用菌学报 2006年第4期 16-20页

作者： FU Li-zhong 1,2, WEI Hai-long 1,2, LI Hai-bo 1,2, WU Qing-qi 1,2, WU Da-feng 2, WU Xue-qian 1,2* 1 Biotechnology research Institute, zhejiang Academy of Forestry science, Hangzhou 310023,china 2Lishui edible fungi research and Development center,zhejiang Essence fungi Development Co., Ltd,Lishui,zhejiang 323000,china

A stable Sequence-Related Amplified Polymorphism （SRAP） molecular marker system for Lentinula edodes has been developed and optimized using genomic DNA extracted from fungal mycelium with sodium dodecyl sulfate-cetyltrimethylammonium bromide （SDS-CTAB）. A large number of amplification products and good reproducibility was achieved using a 20 μL reaction system consisting of 2 μL 10×Reaction Buffer（containing 1.5 mM Mg 2+）, 25 ng genomic DNA, 200 μM dNTP mixture, 1.0 mM Mg 2+, 0.4 μM forward and reverse primer （Me1 and Em16）, 1.5 U Taq polymerase, and ddH_ 2O to volume.

关键词： Lentinula edodes Molecular marker Optimization of amplification protocol SRAP

Authentication of Fungal Mycelium Isolated from Boletus appendiculatus Fruit Body Tissue Using RAPD and ITS Markers

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食用菌学报 2007年第1期 6-9页

作者： LI Haibo1,WU Xueqian1,2,YING Guohua3,WEI Hailong1,2,FU Lizhong1,2,WU Qingqi1,2,XU Lin1,LIU Qing21Biotechnology Institute, zhejiang Academy of Forestry science, Hangzhou, zhejiang 310023, china 2Lishui edible fungi research and Development center, zhejiang Essence fungi Development Co., Ltd,Lishui, zhejiang 323000, china 3Lishui Academy of Forestry science, Lishui, zhejiang 323000, china

RAPD molecular markers and ITS sequence comparisons were used to establish the relationship between Boletus appendiculatus fruit bodies collected from qingyuan, zhejiang Province, and mycelium isolated from the fruit body tissue. RAPD analysis of extracted genomic DNA revealed that B. appendiculatus fruit bodies (fb) and the isolated fungal mycelium (mb) formed a single cluster with a Dice coefficient value of 0.92. Comparison of ITS sequences from these samples showed that base sequences and fragment lengths were identical, thereby confirming that the fungal mycelium isolated from B. appendiculatus fruit bodies was the vegetative form of the mushroom. Our data suggest that RAPD fingerprinting combined with ITS sequence comparisons constitute an effective and feasible method for identifying mycelium isolated from ectomycorrhizal fungi.

关键词： Boletus appendiculatus Identification Molecular marker Pure culture