Optimization of a Sequence-Related Amplified Polymorphism (SRAP) Amplification System for Lentinula edodes

作     者：FU Li-zhong 1,2, WEI Hai-long 1,2, LI Hai-bo 1,2, WU Qing-qi 1,2, WU Da-feng 2, WU Xue-qian 1,2* 1 Biotechnology Research Institute, Zhejiang Academy of Forestry Science, Hangzhou 310023,China 2Lishui Edible Fungi Research and Development Center,Zhejiang Essence Fungi Development Co., Ltd,Lishui,Zhejiang 323000,China 

出 版 物：《食用菌学报》 (Acta Edulis Fungi)

年 卷 期：2006年第4期

页      面：16-20页

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：Supported by Key Technologies R&D Programs of Zhejiang Province(No.2005C22057and2004C22032) andKey Programof Zhejiang Academy of Forestry Science(No.2006F11003) 

主　　题：Lentinula edodes Molecular marker Optimization of amplification protocol SRAP 

摘      要：A stable Sequence-Related Amplified Polymorphism （SRAP） molecular marker system for Lentinula edodes has been developed and optimized using genomic DNA extracted from fungal mycelium with sodium dodecyl sulfate-cetyltrimethylammonium bromide （SDS-CTAB）. A large number of amplification products and good reproducibility was achieved using a 20 μL reaction system consisting of 2 μL 10×Reaction Buffer（containing 1.5 mM Mg 2+）, 25 ng genomic DNA, 200 μM dNTP mixture, 1.0 mM Mg 2+, 0.4 μM forward and reverse primer （Me1 and Em16）, 1.5 U Taq polymerase, and ddH_ 2O to volume.