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FQ-PCR检测宫颈涂片剩余细胞内HPV16、18型感染的临床意义

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实用妇产科杂志 2004年第2期20卷 80-82页

作者：瞿文珍邹先进郑飞云詹丽飞胡燕彭英温州医学院附属第一医院浙江温州325000 湖北省荆门市第一人民医院湖北荆门448000 四川大学华西第二医院四川成都610041

目的 :探讨实时荧光定量聚合酶链反应 (FQ PCR)检测宫颈涂片剩余细胞内人乳头瘤病毒 (HPV) 16、18型感染 ,建立快速、灵敏的检测方法的临床意义。方法 :采用FQ PCR技术检测 12 4例异常宫颈细胞和组织标本HPV16、18型感染状况。结果 :细... 详细信息

目的 :探讨实时荧光定量聚合酶链反应 (FQ PCR)检测宫颈涂片剩余细胞内人乳头瘤病毒 (HPV) 16、18型感染 ,建立快速、灵敏的检测方法的临床意义。方法 :采用FQ PCR技术检测 12 4例异常宫颈细胞和组织标本HPV16、18型感染状况。结果 :细胞学诊断低度鳞状上皮内病变 (LSIL) 4 5例 ,其中FQ PCR检测宫颈细胞标本HPV16、18型DNA阳性14例 (阳性率 31.1% ) ;高度鳞状上皮内病变 (HSIL) 38例 ,HPV16、18型DNA阳性 2 6例 (6 8.4 % ) ;未明确诊断意义的不典型鳞状上皮细胞 (ASCUS) 30例 ,HPV16、18型DNA阳性 10例 (33.3% ) ;鳞状细胞癌 9例 ,HPV16、18型DNA阳性 8例 (88.9% )。组织学检查诊断CINⅠ级 6 7例 ,其中FQ PCR检测活检组织标本HPV16、18型DNA阳性 2 8例 (阳性率 4 1.8% ) ;CINⅡ 14例 ,HPV16、18型DNA阳性 9例 (6 4 .3% ) ;CINⅢ级 11例 ,HPV16、18型DNA阳性 8例 (72 .7% ) ;鳞状细胞癌 2 3例 ,HPV16、18型DNA阳性 17例 (73.9% )。细胞和活检组织两种标本检出的HPV16、18型DNA阳性率差异无显著性 (χ2=1.5 4 ,P >0 .0 5 )。结论 :FQ PCR方法检测宫颈细胞标本HPV16、18型感染 ,具有快速、简便、灵敏度高、特异性强等优点。

关键词： FQ-PCR 宫颈细胞涂片临床意义人乳头瘤病毒细胞学诊断宫颈癌

Effects of vasoactive intestinal peptide on phenotypic and functional maturation of dendritic cells

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Effects of vasoactive intestinal peptide on phenotypic and f...

第十七次全国中西医结合肝病学术会议

作者： Jie Lu~a Ming-Hua Zheng~a Jie Yan~b Yong-Ping Chen~(a,§), Jian-Ping Pan~(b,§) a department of Infectious and Liver Diseases, First affiliated hospital, wenzhou medical college, wenzhou 323000, P R. china b department of Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou 310058, P R. china

The present study was designed to investigate the effects of vasoactive intestinal peptide (VIP) on differentiation, maturation of dendritic cells (DCs) in vitro. DCs were derived from the murine bone marrow hemopoietic progenitor cells by culturing in RPMI 1640 complete medium supplemented with GM-CSF and IL-4 in the presence or absence of various concentrations of vasoactive intestinal peptide (VIP) and lipopolysaccharide (LPS) The phenotype of DCs was analyzed by flow cytometry. Mixed leukocyte reaction (MLR) was employed to measure the capacity of DC to stimulate the allogeneic T cells. IL-12p70 secretion by DC was examined by ELISA. In the absence of LPS, VIP, in a dose dependent manner, up-regulated the expression of CDS0, CD86, CD54 and CD40, but down-regulated the expression of MHC class Ⅱ molecule (Ia). In the presence of LPS, VIP also dose dependently up-regulated the expression of CD80, CD86, CD54 and CD40, and down-regulated the expression of Ia. The capacity to stimulate alloreactive T cells and the production of IL-12p70 by DC were significantly augmented by VIP when compared with VIP-untreated DCs. These data suggest that VIP could promote the phenotypic and functional maturation of DCs, hereby regulating the type and outcome of the conducting immune response.

关键词： Dendritic cells Mixed leukocyte reaction IL-12 Costimulatory molecules Vasoactive intestinal peptide

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65例胎儿肾积水的超声诊断与预后观察

实用妇产科杂志 2011年第12期27卷 912-914页

作者：黄湖王亮寇红菊罗洪霞温州医学院附属第二医院浙江温州325027

目的:探讨胎儿肾积水的超声诊断和预后情况。方法:对65例孕龄33～40周的胎儿81个肾积水按肾盂前后径值(APD)法进行分级(Ⅰ级～Ⅳ级),出生后1月、3月、6月超声检查随访肾积水的情况,对疾病进展的患儿随访至10个月,经手术治疗的患儿术后1... 详细信息

目的:探讨胎儿肾积水的超声诊断和预后情况。方法:对65例孕龄33～40周的胎儿81个肾积水按肾盂前后径值(APD)法进行分级(Ⅰ级～Ⅳ级),出生后1月、3月、6月超声检查随访肾积水的情况,对疾病进展的患儿随访至10个月,经手术治疗的患儿术后1个月后随访1次。结果:81个肾积水中72个肾积水为生理性(88.9%)。Ⅰ级41个肾均于出生后6个月内好转,其中1个月内好转30个肾;Ⅱ级27个肾均于6个月内好转,其中3个月内好转16个肾;Ⅲ级6个肾中有2个进展;Ⅳ级7个肾中5个行手术治疗,手术治疗的肾积水占6.2%(5/81)。有进展需继续观察的Ⅲ级和Ⅳ级肾积水各2个,占4.9%(4/81),其他影像学检查均为较轻肾盂输尿管连接部狭窄,暂无手术指征。结论:胎儿肾积水大多数是生理性,仅少部分为泌尿系畸形需手术治疗,且多为Ⅳ级肾积水。超声检查是诊断胎儿肾积水和判断预后最佳手段。

关键词：胎儿肾积水超声检查预后

UroplakinⅢ在侵袭性膀胱癌中的表达及其临床意义

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实用肿瘤杂志 2010年第2期25卷 180-183页

作者：黄伟平翁志梁余志贤万丽吴秀玲温州医学院附属第一医院泌尿外科浙江温州325000 温州医学院附属第一医院病理科浙江温州325000

目的研究UroplakinⅢ(UPⅢ)在侵袭性膀胱癌(aggressive bladder cancer)中的表达情况及意义。方法选取74例侵袭性膀胱癌根治性膀胱切除术后的标本和10例正常膀胱组织,用免疫组织化学的方法检测UPⅢ的表达情况及膀胱癌组织中微血管密度(m... 详细信息

目的研究UroplakinⅢ(UPⅢ)在侵袭性膀胱癌(aggressive bladder cancer)中的表达情况及意义。方法选取74例侵袭性膀胱癌根治性膀胱切除术后的标本和10例正常膀胱组织,用免疫组织化学的方法检测UPⅢ的表达情况及膀胱癌组织中微血管密度(microvascular density,MVD),并分析其与侵袭性膀胱癌的临床病理学指标关系。结果UPⅢ在侵袭性膀胱癌中的表达与在正常膀胱组织中表达相比差异有统计学意义(P25例表达阳性,其表达缺失与膀胱癌高分级、肌层浸润、淋巴管浸润呈正相关(P<0.05),侵袭性膀胱癌组织中UPⅢ阴性表达组的MVD明显高于UPⅢ阳性表达组(P<0.01)。结论UPⅢ表达缺失与侵袭性膀胱癌的病理分级、分期及淋巴管浸润有相关性;UPⅢ可促进肿瘤血管形成从而进一步促进肿瘤细胞的生长。

关键词：膜糖蛋白类膀胱肿瘤/病理学免疫组织化学新生血管化,病理性癌,移行细胞/病理学

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多排螺旋CT低剂量扫描在小儿胸部的应用

实用放射学杂志 2007年第6期23卷 792-794页

作者：孔秋雁陈廷港许崇永陈宇吴爱琴温州医学院附属第二医院CT室温州医学院附属第二医院CT室浙江温州325027

目的评估低剂量扫描在小儿胸部的应用。方法水模测试:在水体模的同一层面做3mm层厚的150、80、50、35、15mAs扫描。在每幅图像的5个相同感兴趣区测CT值,做CT值的均匀性和噪声水平比较。病例扫描:随机选择肺部感染的患儿40例,先行常规剂... 详细信息

目的评估低剂量扫描在小儿胸部的应用。方法水模测试:在水体模的同一层面做3mm层厚的150、80、50、35、15mAs扫描。在每幅图像的5个相同感兴趣区测CT值,做CT值的均匀性和噪声水平比较。病例扫描:随机选择肺部感染的患儿40例,先行常规剂量(150mAs)扫描以确定感染部位,再在感染局部做低剂量扫描(50,35mAs及15mAs)。其他参数为:120kV,床进28.8mm/圈,0.5s/圈,16×1.5mm准直,重建层厚及间隔均为3mm。比较不同剂量的CT权重剂量指数(CTDIw)及剂量长度乘积(DLP);由2位高年资医师按优、良、合格及不合格的等级盲法评价不同剂量的图像质量,结果进行统计学处理。结果CT图像的均匀性随着扫描剂量的减少而变差,图像噪声水平随扫描剂量的减少而增高。小儿胸部150、50、35、15mAs的CTDIw分别为:10.0、3.3、2.3、1.0mGy,其DLP分别为:117.8、39.3、27.5和11.8;150、50、35mAs的符合临床诊断图像(包括优,良,合格3个等级)所占比例,肺窗和纵隔窗均不小于90%,而15mAs的不合格图像所占比例为20%。结论在保证图像质量的前提下,多排螺旋CT小儿胸部检查采用35mAs的扫描条件较为适宜。

关键词：小儿胸部体层摄影术,X线摄影术

Treatment of invasive giant prolactinomas involving the cavemous sinus with Bromocriptine:Results of long-term follow-up

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Treatment of invasive giant prolactinomas involving the cave...

中国医师协会神经外科医师分会首届全国代表大会

作者： Zhe Bao Wu Chun Jiang Yu Zhi Peng Su Qi Chuan Zhuge Jin Sen Wu Wei Ming Zheng department of Neurosurgery, First affiliated hospital of wenzhou medical college, wenzhou 325000, People'' s Republic of china (Wu ZB, Su ZP, Zhuge QC, Wu JS, Zheng WM) and department of Neurosurgery, Fu Xing hospital, Capital University of medical Science, Bei Jing 100038, People'' s Republic of china (Yu CJ)

＜正＞Objective To observe the long- term clinical outcomes in a group of patients with invasive giant prolactinomas （IGPs） involving the cavernous sinus treated with bromocriptine. Methods Data from 20 patients with IGPs treated in our institutions during a period from July, 1997 to June, 2004 were retrospectively reviewed. The criteria utilized included: （1） tumor diameter ＞ 4 cm, invasion of the cavernous sinus by tumor, corresponding to grade III - IV according to the classification of Knosp;（2） serum prolactin level ＞ 200 ng/ml;（3） clinical signs of hyperprolactinemia and mass effect. Among the 20 patients who met the criteria: 6 patients had undergone unsuccessful transcranial or transsphenoidal microsurgery prior to bromocriptine treatment and 14 patients initially received bromocriptine as primary treatment. Eleven of the 20 had received adjuvant radiotherapy. Results After an average follow- up period of 37. 3 months, the clinical symptoms of all patients had improved by different degrees. Tumor volume on MRI was decreased on average by 93. 3 %;in 11 patients, the tumors had almost completely disappeared, and in the other 9 patients, residual tumor involved the cavernous sinus. Visual symptoms improved in 13 of the patients who presented with visual loss. Eight patients had normal PRL level. The postoperative prolactin level was more than 200ng/ml in 7 patients. During the course of drug administration, CSF leakage occurred in one patient, who subsequently underwent transsphenoidal surgery. No cases of apoplexy occurred during bromocriptine treatment. Conclusion Dopamine agonist medications are effective as first- line therapy for IGPs, because they can significantly shrink tumor volume and control the PRL level. Tumor mass vanishes in some patients after bromocriptine treatment, and for other patients, with localized residual tumor, stereotaxic radiosurgery is an option so that unnecessary surgery can be avoided. Radiotherapy does not reliably result in tumor

关键词： Giant prolactinoma Invasiveness Bromocriptine Cavernous sinus

白藜芦醇在大鼠离体心脏低温保存中的保护作用及其机制

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中国应用生理学杂志 2014年第4期30卷 348-351页

作者：孙玮明郑鸣之应磊虞晓明吴斯文陈莹莹沈岳良汪洋温州医科大学病理生理学教研室浙江温州325035 温州医科大学附属第一医院超声影像科浙江温州325035 浙江医学高等专科学校药理教研室杭州310053 温州医科大学附属第一医院呼吸科浙江温州325035 浙江大学医学院病理生理学教研室杭州310058

目的:探讨白藜芦醇(RES)对长时程低温保存的大鼠供心是否具有保护作用及其机制是否通过调节Sirt-1的表达。方法:SD大鼠随机分为以下7组,包括空白对照组(control),单纯9 h低温保存组(9 h),RES组(3、10、30μmol/L RES组),尼克酰胺组(NAM... 详细信息

目的:探讨白藜芦醇(RES)对长时程低温保存的大鼠供心是否具有保护作用及其机制是否通过调节Sirt-1的表达。方法:SD大鼠随机分为以下7组,包括空白对照组(control),单纯9 h低温保存组(9 h),RES组(3、10、30μmol/L RES组),尼克酰胺组(NAM:保存液中加入Sirt-1抑制剂尼克酰胺NAM 40μmol/L),白藜芦醇+尼克酰胺组(RES+NAM:保存液中加入30μmol/L RES和40μmol/L NAM)。采用大鼠离体心脏Langendorff灌流模型,HE染色观察心肌细胞在各组中的形态学变化;用Real-TimePCR和Western blot法分别检测Sirt-1基因和蛋白在各组中的表达。结果:1与空白对照组相比,9 h组心肌细胞损伤明显加重,Sirt-1基因和蛋白表达均明显下调(P2与9 h组相比,3、10、30μmol/L RES组心肌细胞损伤逐渐减轻,Sirt-1表达水平呈剂量依赖性升高(P<0.05);3RES的心肌细胞保护作用可被Sirt-1抑制剂NAM所取消。结论:RES能够改善长时程低温保存引起的心肌细胞损伤,这种保护作用可能通过上调Sirt-1的表达来实现。

关键词：心脏低温保存白藜芦醇 Sirt-1 大鼠

Impact of invasive fungal infection on the outcomes of severe sepsis:A multicenter study in critically I11 surgical patients

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Impact of invasive fungal infection on the outcomes of sever...

浙江省免疫学会第六次学术研讨会

作者： GuoHao Xie~* XiangMing Fang~* Qiang Fang~* XinMin Wu~+ YuHong Jin~(++) JunLu Wang~§ QuLian Guo~(?) MiaoNing Gu~(?) QiuPing Xu~(**) DongXin Wang~+ ShangLong Yao~(++) ShiYing Yuan~(++) ZhaoHui Du~(++++) YunBo Sun~(§§) HaiHong Wang~(**) ShuiJing Wu~* BaoLi Cheng~* QiXing Chen~* * department of Anesthesiology and Intensive Care Unit,the first affiliated hospital,School of Medicine,Zhejiang University,Hangzhou,china * department of Anesthesiology and Intensive Care Unit,the first affiliated hospital,School of Medicine,Peking University,Beijing,china ++ Intensive Care Unit,Lihuili hospital,School of Medicine,Ningbo University,Ningbo,china § department of Anesthesiology,the First affiliated hospital,wenzhou medical college,wenzhou,china (?) department of Anesthesiology and Intensive Care Unit,Xiangya hospital,Xiangya medical college,Central South University,Changsha,china department of Anesthesiology and Intensive Care Unit,South hospital,South medical University,Guangzhou,china ** department of Anesthesiology and Intensive Care Unit,Sir Run Run Shaw hospital,School of Medicine,Zhejiang University,Hangzhou,china ** department of Anesthesiology and Intensive Care Unit,Union hospital,Tongji Medicine college,Huazhong University of Science and Technology,Wuhan,china ++++ Intensive Care Unit,Zhongnan hospital,School of Medicine,Wuhan University,Wuhan,china: §§ department of Anesthesiology and Intensive Care Unit,Qingdao university hospital,School of Medicine,Qingdao University,Qingdao,china

Objective:To investigate the impact of invasive fungal infection (IFI) on the outcomes of critically ill surgical patients with severe sepsis in multiple medical centers within *** Background Data:Fungal infection has been more common in severe sepsis and be one of the risk factors affecting survival in Intensive Care ***:The records

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Rifaximin Versus Non-absorbable Disaccharides in the Management of Hepatic Encephalopathy

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Rifaximin Versus Non-absorbable Disaccharides in the Managem...

2009香港-北京-杭州内科论坛暨2009年浙江省内科学学术年会

作者： department of Infection and Liver Diseases,The First affiliated hospital of wenzhou medical college,wenzhou,china Ming-Hua Zheng Dian-Na Gu Lei Zhang Yong-Ping Chen Base for Drug Clinical Trial / GCP Center,West china hospital, Sichuan University,Chengdu,china Mao-Bin meng Yong-Ping Chen department of Clinical Pharmacy,West china School of Pharmacy, Sichuan University,Chengdu,china Qian Jiang Yong-Ping Chen

Objective:To compare rifaximin with non-absorbable disaccharides in treating patients with hepatic encephalopathy(HE). Methods:We performed a meta-analysis using Cochrane Collaboration guidelines of comparative random... 详细信息

关键词： rifaximin non-absorbable disaccharides hepatic encephalopathy meta-analysis