检索结果-南通市图书馆

Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

在线全文

学校读者我要写书评

暂无评论

Science china chemistry 2010年第4期53卷 812-819页

作者： LI ZhiYong1, ZHANG QianYun2, ZHAO LiXia1, LI ZhenJia3, HU GuoMao3, LIN Jin-Ming2 & WANG Shan4 1State Key Laboratory of Environmental chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, beijing 100085, china 2department of chemistry, tsinghua university, beijing 100084, china 3beijing Chemclin Biotech Co., Ltd., beijing Academy of Science and Technology, beijing 100094, china 4School of chemistry and Chemical Engineering, Xianyang Normal College, Xianyang 712000, china 1. State Key Laboratory of Environmental chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China2. Department of Chemistry Tsinghua University Beijing 100084 China3. Beijing Chemclin Biotech Co. Ltd. Beijing Academy of Science and Technology Beijing 100094 China4. School of Chemistry and Chemical Engineering of Xianyang Normal College Xianyang 712000 China

A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

关键词： micro-plate magnetic chemiluminescence immunoassay carcinoembryonic antigen tumor marker

Determination of Estrogens and Bisphenol A in Bovine Milk by Automated on-line C30 Solid-phase Extraction Coupled with High-performance Liquid Chromatography-mass Spectrometry

在线全文

学校读者我要写书评

暂无评论

Determination of Estrogens and Bisphenol A in Bovine Milk by...

A MESSAGE-DRIVING FORMALISM FOR MODELING AND SIMULATION OF MULTI-AGENT SUPPLY CHAIN SYSTEMS

第五届全国环境化学大会

作者： Wei Yan~1 Ying Li~1 Lixia Zhao~1 Jinming Lin~(1,2,*) (1 State Key Laboratory of Environmental chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,P.O.Box 2871,beijing 100085, china 2department of chemistry,tsinghua university,beijing 100084,china

Using triacontyl bonded silica(C) as on-line solid-phase extraction(SPE) material and a specially designed online analytical system which allowed large sample volume injection,a high speed and robust online SPE-HPLC-MS method was established for the analysis of five estrogens and bisphenol A(BPA) in milk *** milk sample is pretreated with acetonitrile for protein precipitation and then treated with primary

关键词： Triacontyl bonded silica Estrogens Liquid chromatography-mass spectrometry Spiked milk samples

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Systems Science and Systems Engineering 2011年第4期20卷 385-399页

作者： Wenzhe TAN1 Yueting CHAI2 Yi LIU3 1department of Automation, tsinghua university, beijing, 100084, china 2department of Automation, tsinghua university, beijing, 100084, china 3department of Automation, tsinghua university, beijing, 100084, china department of Automation Tsinghua University Beijing China

To acquire a competitive advantage in the expanding market, manufacturing enterprises should be able to manage their supply chains as effectively as possible. It is now becoming popular to model supply chains as multi-agent systems and use discrete event simulation to learn more about their behaviors or investigate the implications of alternative configurations. In order to enhance the computational efficiency and keep the simulation credibility, this paper proposes a message-driving formalism for the simulation of multi-agent supply chain systems. Through the message-driving formalism, the problem of shared variables is addressed and the parallel operation of agents is implemented. Simulation experiments with a prototype implementation show that the message-driving formalism is able to provide credible results in significantly less simulation time.

关键词： Supply chain multi-agent system modeling and simulation parallel discrete event simulation message-driving formalism

Closed and endoreversible chemical oscillation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of chemistry 1994年第3期12卷 199-205页

作者： LI, Ru-Shengdepartment of chemistry, tsinghua university, beijing 100084, china department of chemistry Tsinghua University Beijing 100084 China

Based on a simple nonideal chemical reaction model, the closed system with reversible nonideal reactions and illumination is shown to be able to exhibit sustained oscillation. The essential points in the occurrence of such oscillations are the nonideality of reacting media, which gives rise to multiple chemical equilibria, and the energy flow through illumination and heat conduction, which gives rise to spontaneous transitions repeatedly between the multiple equilibrium states. During the oscillation, reacting media undergo only reversible chemical transformation and all irreversibilities are restricted to the coupling of the system to external world. This oscillation provides an example of the so-called endoreversible machine and a possible way to convert radiation energy of the light directly into mechanical energy.

关键词： Chemical oscillation closed system nonideality endoreversible machine illumination.

SOME PROPERTIES OF LSQR FOR LARGE SPARSE LINEAR LEAST SQUARES PROBLEMS

在线全文

学校读者我要写书评

暂无评论

Journal of Systems Science & Complexity 2010年第4期23卷 815-821页

作者： Zhongxiao JIA department of Mathematical Sciences,tsinghua university,beijing 100084,china. department of Mathematical Sciences Tsinghua University

It is well-known that many Krylov solvers for linear systems,eigenvalue problems,andsingular value decomposition problems have very simple and elegant formulas for residual *** not only allow us to further understand the methods theoretically but also can be usedas cheap stopping criteria without forming approximate solutions and residuals at each step beforeconvergence takes *** for large sparse linear least squares problems is based on the Lanczosbidiagonalization process and is a Krylov ***,there has not yet been an analogouslyelegant formula for residual *** paper derives such kind of *** addition,the authorgets some other properties of LSQR and its mathematically equivalent CGLS.

关键词： CGLS krylov subspace lanczos bidiagonalization least squares lsqr normal equations.

THE EXISTENCE OF J-HOLOMORPHIC CURVES AND APPLICATIONS TO THE WEINSTEIN CONJECTURE

在线全文

学校读者我要写书评

暂无评论

Chinese Annals of Mathematics,Series B 1999年第4期20卷 425-434页

作者： MA RENYI(department of Applied Mathmatics, tsinghua university, beijing 100084, china) tsinghua Univ Dept Appl Math Beijing 100084 Peoples R China

The author first proves the existences of J-holomorphic curves in the symplectizations of Legendre fibrations and then as an application confirms the Weinstein conjectures on contact manifolds of Legendre fibrations. As a corollary a new proof on the theorem due to Hofer,Viterbo, Gluck, Ziller, Weinstein and Ljusternik-Fet Theorem is provided, which is quite different from their original proofs.

关键词： Symplectic geometry J-holomorphic curves Periodic orbit

SYNTHESIS OF NOVEL SOLUBLE AROMATIC POLYPYROMELLITIMIDE CONTAINING ORDERED 1,2,4-TRIAZINE IN THE MAIN CHAIN

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2002年第3期20卷 219-224页

作者： Xiao-bing Yi Guo-shi Wu Feng-cai Lu department of chemistry tsinghua university, beijing 100084 china Institute of chemistry Chinese Academy of Sciences, beijing 100080, china Tsing Hua Univ Dept Chem Beijing 100084 Peoples R China Chinese Acad Sci Inst Chem Beijing 100080 Peoples R China

A new heat-resistant, soluble polymer, poly(phenyl-1,2,4-triazine imide), has been prepared via a two-step method of polycondensation of N,N'-bis(4-benzil)pyromellitimide and terephthalamidrazone in cresol, followed by curing at higher temperature. The rigid 1,2,4-triazine group increases the solubility of the polyimide so that the polymer is soluble in cresol, N-methyl-2-pyrrolidone (NMP), trifluoroacetic acid and concentrated sulfuric acid, etc. Thermogravimetric analysis (TGA) indicates that this novel polyimide has good thermal stability with a high decomposition temperature of 447degreesC in air and of 423 degreesC in N-2, It has a tensile strength of 107.4 MI'a with an elongation at break of around 7.6%.

关键词： polyimides N,N '-bis(4-benzil)pyromellitimide rigid-rod group 1,2,4-triazine

TEXTURE DETERMINATION OF THIN FILM ZrO_2 BY ELECTRON DIFFRACTION

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 1993年第12期6卷 472-473页

作者： WANG Yinghua tsinghua university,beijing,china department of Materials Science and Engineering,tsinghua university,beijing 100084,china tsinghua Univ Beijing China

A method for semi-quantitative determination of thin film texture using electron diffrac- tion is *** texture state of a zirconia thin film with a cubic structure has been determined in this way and is presented as an... 详细信息

关键词： texture thin film electron diffraction ZrO_2

DIMENSIONS OF SELF-AFFINE SETS WITH OVERLAPS

在线全文

学校读者我要写书评

暂无评论

Chinese Annals of Mathematics,Series B 2003年第3期24卷 275-284页

作者： HUA Su department of Mathematics, tsinghua university, beijing 100084, china. Tsing Hua Univ Dept Math Beijing 100084 Peoples R China

The authora develop an algorithm to show that a class of self-affine sets with overlaps can be viewed as sofic affine-invariant sets without overlaps,thus by using the results of [11] and [10],the hausdorff and minkow... 详细信息

关键词： self-affine set overlap hausdorff dimension minkowski dimension