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Analysis of the Genetic Relationships in Chinese Ziziphus with SRAP Markers

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agricultural sciences in china 2010年第9期9卷 1278-1284页

作者： LI Li, PENG Jian-ying and BAI Rui-xia college of Horticulture, agricultural university of hebei, baoding 071001, P.R.china college of Horticulture Agricultural University of Hebei Baoding 071001 P.R. China

The phylogenic and genetic relationships in germplasm resources of Chinese Ziziphus were studied at molecular levels, for providing new molecular evidences of classification, protection, and utilization of germplasm resources of Ziziphus. The sequence-related amplified polymorphism （SRAP） was assessed to analyse the genetic relationships among 14 species of Ziziphus, 11 cultivars of Z. jujuba Mill., and one outgroup. A total of 580 DNA bands were amplified by 19 selective primers, 570 of which （98.28%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 30. The genetic similarities of 26 sample materials were between 0.22 and 0.99. UPGMA method cluster analysis showed that 26 sample materials were classified into six cluster groups with the genetic similarity of 0.28. The results showed that SRAP technique is efficient in studying genetic relationships among Ziziphus, Z. jujuba Mill. and Z. acidojujuba C. Y. Cheng et M. J. Liu which should be treated as one species, and further infraspecific classification of Z. jujuba Mill. should be classified into two subspecies; the scientific names of new taxa, Chinese jujube, and wild jujube were Ziziphus jujuba Mill. subsp, jujuba and Ziziphus jujuba Mill. subsp, spinosa （Bunge） J. Y. Peng, X. Y. Li et L. Li, respectively. Z. xiangchengensis Y. *** et P. K. Chou, Z. montana W.W. Smith and Z. mairei Dode might be originated from same ancestry. Z xiangchengensis Y. L. Chen et P. K. Chou and Z. montana W.W. Smith should be treated as one species. The infrasubspecific taxon of Z. jujuba Mill. was not suitable to set up varieties.

关键词： Ziziphus Mill. genetic relationships SRAP cluster analysis

Effects of spider Macrothele raven venom on cell proliferation and cytotoxicity in HeLa cells

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Acta Pharmacologica Sinica 2005年第3期26卷 369-376页

作者： Li GAO~(2,3,9) Bao-en SHAN~4 Jing CHEN~5 Jiang-hui LIU~6 Da-xiang SONG~2 Bao-cheng ZHU~(2,7,8)2 college of Life science,hebei university,baoding 071002,china 3 college of Life science,hebei Normal university,Shijiazhuang 050016,china 4 Research Centre,The Fourth Hospital of hebei Medical university,Shijiazhuang 050011,china 5 Clinical Laboratory,Bethune International Peace Hospital,Shijiazhuang 050082,china 6 hebei Provincial Tumor Institute,Shijiazhuang 050011,china 7 college of Life science,agricultural university of hebei,baoding 071001,china college of Life science Hebei University Baoding China college of Life science Hebei Normal University Shijiazhuang China Research Centre The Fourth Hospital of Hebei Medical University Shijiazhuang China Clinical Laboratory Bethune International Peace Hospital Shijiazhuang China hebei Provincial Tumor Institute Shijiazhuang China college of Life science Agricultural University of Hebei Baoding China

Aim:To examine the effect of venom from the spider Macrothele raven on cellproliferation and cytotoxicity in human cervical carcinoma,HeLa ***:Morphological and biochemical signs of apoptosis appeared using acridine or-ange-ethidium bromide （AO/EB） *** morphological changes in HeLacells alter treatment with spider venom were observed using scanning electronmicroscopy （SEM） and transmission electron microscopy （TEM）.Cell prolifera-tion and cytotoxicity were determined by [methyl-3H] thymidine assay （[3H]TdR）and lactate dehydrogenase （LDH） release,*** fragmentation andcell cycle distribution were monitored using flow *** addition,Westernblot analysis was used to evaluate the level of caspase-3 *** vivoexamination of the inhibition of the size of tumors in nude mice treated with spidervenom was ***:Marked morphological changes were observedusing AO/EB staining,SEM and TEM *** venom at concentrations of10-40 mg/L caused dose- and time-dependent inhibition of HeLa cell *** ratio of apoptosis and necrosis *** activity of caspase-3 wasupregulated after spider venom *** vivo study of tumor size revealedthat tumors significantly decreased in size from controls to tumors treated for 3weeks with spider venom （P<0.05）.Conclusion:The inhibition of HeLa cells bythe venom of the spider Macrothele raveni was carried out in three ways:induc-tion of apoptosis,necrosis of toxicity damage and direct *** venom is anovel anti-tumor material both in vitro and in vivo.

关键词： HeLa cells spider venom cell proliferation cytotoxicity cell cycle caspase-3 expression

Research progress in the study of atmospheric pressure glow barrier discharge

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Progress in Natural science:Materials International 2007年第3期17卷 247-253页

作者： LI Xuechen, DONG Lifang and JIA Pengying (college of Physics science and Technology, hebei university, baoding 071002, china) college of Physics science and Technology Hebei University Baoding 071002 China

Atmospheric pressure glow barrier discharge (APGBD) can operate at high pressure, and so vacuum device is not necessary. Furthermore, the produced plasma by APGBD has moderate electron temperature and density besides good uniformity. Therefore, APGBD has extensive potential applications in industry and has been becoming a hot issue in the research of low temperature plasma. In this paper, the main problems in the study of atmospheric pressure glow discharge generated by dielectric barrier discharge, including the experimental setup, judging criterion, discharging conditions, physical mechanisms, and parameter diagnoses, are discussed, and further research prospects of APGBD are proposed.

关键词： atmospheric pressure glow discharge dielectric barrier discharge low temperature plasma.

Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

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Progress in Natural science:Materials International 2007年第12期17卷 1419-1424页

作者： Zhang Lifan1, Liu Chong2, Lian Linsheng3, Zhao Chunjiang1, Bao Haigang1 and Wu Changxin1(1. college of animal science and Technology, china agricultural university, Beijing 100094, china 2. college of Biological science, china agricultural university, Beijing 100094, china 3. college of animal science and Technology, Yunnan agricultural university, Kunming 650201, china) college of animal science and Technology China Agricultural University Beijing 100094 China college of Biological science China Agricultural University Beijing 100094 China college of animal science and Technology Yunnan Agricultural University Kunming 650201 China

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2224(E4)-Asn and Ser-2279(E4)-Gly were identified in exon 4 of the gene. The Lys-2224(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2224(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.

关键词： Tibet chicken neuroglobin hypoxia adaptation Ngb gene mutation.

Mixed boundary conditions for piezoelectric plates

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science china(Physics,Mechanics & Astronomy) 2009年第5期52卷 755-761页

作者： GAO Yang 1 ,XU SiPeng 2 &ZHAO BaoSheng 3 1college of science,china agricultural university,Beijing 100083,china 2college of Engineering,Ocean university of china,Qingdao 266071,china 3 School of Mechanical Engineering,university of science and Technology of Liaoning,Anshan 114044,china college of science China Agricultural University Beijing China college of Engineering Ocean University of China Qingdao China School of Mechanical Engineering University of Science and Technology of Liaoning Anshan China

For plate bending and stretching problems in piezoelectric materials,the reciprocal theorem and the general solution of piezoelasticity are applied in a novel way to obtain the appropriate mixed boundary conditions accurate to all order.A decay analysis technique is used to establish necessary conditions that the prescribed data on the edge of the plate must satisfy in order that it should generate a decaying state within the *** the case of axisymmetric bending and stretching of a circular plate,these decaying state conditions are obtained explicitly for the first time when the mixed conditions are imposed on the plate *** are then used for the correct formulation of boundary conditions for the interior solution.

关键词： boundary conditions piezoelectric plates mixed data bending problems stretching problems

Statistical theory for hydrogen bonding fluid system of A_aD_d type (I): The geometrical phase transition

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science china Chemistry 2006年第6期49卷 499-506页

作者： WANG Haijun1,2, HONG Xiaozhong3, GU Fang1 & BA Xinwu1 1. college of Chemistry and Environment science, hebei university, baoding 071002, china 2. International Center for Materials Physics, Chinese Academy of sciences, Shenyang 110016, china 3. college of Physics science and Technology, hebei university, baoding 071002, china college of Chemistry and Environment science Hebei University Baoding China International Center for Materials Physics Chinese Academy of Sciences Shenyang China college of Physics science and Technology Hebei University Baoding China

The influence of hydrogen bonds on the physical and chemical properties of hydrogen bonding fluid system of AaDd type is investigated from two viewpoints by the principle of statistical mechanics. In detail, we proposed two new ways that can be used to obtain the equilibrium size distribution of the hydrogen bonding clusters, and derived the analytical expression of a relationship between the hydrogen bonding free energy and hydrogen bonding degree. For the nonlinear hydrogen bonding systems, it is shown that the sol-gel phase transition can take place under proper conditions, which is further proven to be a kind of geometrical phase transition rather than a thermodynamic one. Moreover, several problems associated with the geometrical phase transition and liquid-solid phase transition in nonlinear hydrogen bonding systems are discussed.

关键词： hydrogen bonding fluid, equilibrium free energy, geometrical phase transition.

Study on Tibetan Chicken embryonic adaptability to chronic hypoxia by revealing differential gene expression in heart tissue

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science china(Life sciences) 2009年第3期52卷 284-295页

作者： LI Mei & ZHAO ChunJiang college of animal science and Technology,china agricultural university,Beijing 100193,china college of animal science and Technology China Agricultural University Beijing China

Oxygen concentration is essential for appropriate *** can exert a significant impact on physiological alteration of the cell and *** Chicken(Gallus gallus) is a Chinese indigenous breed inhabiting in Tibetan areas,which is also a chicken breed living at high altitude for the longest time in the *** has developed an adaptive mechanism to hypoxia,which is demonstrated by that Tibetan Chicken has much higher hatchability than low-land chicken breeds in high-altitude areas of *** the present study,Tibetan Chicken fertilized full sib eggs were incubated up to Hamburger-Hamilton stage 43 under 13% and 21% oxygen concentration,*** Chicken and Dwarf Recessive White Chicken were used as control *** hearts in all of the 3 chicken breeds under hypoxic and normoxic conditions were isolated and hybridized to Genechip Chicken Genome Array to study molecular mechanisms underlying the adaptation to high altitude of Tibetan *** a result,50 transcripts highly expressed in hypoxia are screened *** up-regulated genes,some are involved in the gene ontology(GO) such as cell growth,cell difference,muscle contraction and signal ***,the expression levels of 21 transcripts are lower in hypoxia than those in *** down-regulated genes take part in cell communication,ion transport,protein amino acid phosphorylation and signal ***,gene enrichment analyses of these differential gene expressions are mainly associated with immune system response and ion channel activity in response to ***,the transcriptional expression profiles analyzed by hierarchical clustering and CPP-SOM software in all of the 3 different chicken breeds revealed that Tibetan Chicken is much closely related to Shouguang Chicken rather than Dwarf Recessive White *** addition,12 transcripts of Tibetan Chicken breed-specific expressed genes were identified,which seem to result in a more

关键词： adaptation chronic hypoxia gene chip chicken genome array Tibetan Chicken(Gallus gallus) differential gene expression

The Effect of Improving soil Fertility on Status of Organo－mineral Complexing in Chestnut soils

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河北农业大学学报 1995年第S1期18卷 41-46页

作者： Liu Shuqing Huo Xiliang(agricultural university of hebei,baoding 071001)

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关键词： Chestnut Complexing

yellow^0,a marker for low body weight in Drosophila melanogaster

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science china(Life sciences) 2009年第7期52卷 672-682页

作者： Li XinHai & Deng XueMei college of animal science and Technology,china agricultural university,Beijing 100193,china college of animal science and Technology China Agricultural University Beijing China

Marker-assisted selection(MAS) is an important modern breeding technique,but it has been found that the effect of the markers for quantitative trait loci(QTL) is inconsistent,leading in some cases to MAS failure and raising doubts about its *** the model organism Drosophila melanogaster was employed to study whether an effective marker could be found and applied to *** crossed the stock carrying the y0 marker(a recessive mutation allele of the yellow gene on the X chromosome) with three other stocks carrying corresponding wild-type markers in an F2 design,and found that the y0 marker was in significant association with low body weight(P20 generations of marker-assisted introgression(MAI),and constructed the introgression stock y0(OR)20 in which body weight decreased by 13% and 7%,in female and male,respectively,compared to the wild stock(P20,indicating that the QTL for low body weight is located within this less-than-1.5 cM *** characteristics of the y0 marker by PCR amplification and Southern blotting revealed that yellow gene was deficient in the y0 stock,leading to disappearance of melanin from the cuticle and probably influencing the developmental *** above results confirmed the existence of effective QTL markers applicable to MAS breeding schemes,and their potential application in breeding new stocks.

关键词： Drosophila low body weight yellow quantitative trait loci marker-assisted selection introgression