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Bio-regeneration of p-complexation desulfurization adsorbents

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Science china Chemistry 2005年第6期48卷 538-544页

作者： LI Wangliang1,2. XING Jianmin1, XIONG Xiaochao1,2. SHAN Guobin1,2.& LIU Huizhou1 1. Laboratory of Separation Science and Engineering, Institute of Process Engineering, chinese academy of sciences, beijing 100080, {2. 2. graduate School of the chinese academy of sciences,beijing 100039, {2. 1. Laboratory of Separation Science and Engineering Institute of Process Engineering Chinese Academy of Sciences 100080 Beijing China 2. graduate School of the chinese academy of sciences 100039 Beijing China

The coupling of adsorption desulfurization and biodesulfurization is a new approach to produce clean fuels. Sulfur compounds are firstly adsorbed on adsorbents, and then the ad-sorbents are regenerated by microbial conversion. p-Complexation adsorbent, Cu(I)-Y, was ob-tained by ion exchanging Y-type zeolite with Cu2. and then by auto-reduction in helium at 450℃ for 3 h. Dibenzothiophene (DBT) was used as a model compound. The effects of cell concentra-tion, volume of oil phase, the ratio of aqueous phase to adsorbent on DBT desorption by a bac-terium were studied. The amounts of DBT desorbed and 2.HBP produced can be apparently increased with addition of n-octane. BDS activity can be improved by increasing cell concentra-tion and the ratio of water-to-adsorbent. 89% of DBT desorbed from the adsorbents can be converted to 2.HBP within 6 h and almost 100% within 2. h, when the volume ratio of oil-to-water was 1/5 mL/mL, the cell concentration was 60 g·L?1, and the ratio of adsorbent-to-oil was 0.03 g·mL?1. The amount of 2.HBP produced was strongly dependent on the volume ratio of oil-to- water, cell concentration and amount of adsorbent. Adsorption capacity of the regenerated ad-sorbent is 95% that of the fresh one after being desorbed with Pseudomonas delafieldii R-8, washed with n-octane, dried at 100℃ for 2. h and auto-reduced in He.

关键词： adsorption desulfurization p-complexation adsorbents bio-regeneration Pseudomonas delafieldii desorption.

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Cloning,expression and characterization of a nucleoside diphosphate kinase (NDPK) gene from tobacco

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Progress in Natural Science:Materials International 2007年第08期17卷 906-912页

作者： Zhou Qiyun~(1,2.*) Xie Zongming~(1**) Zhang Zhigang~1 Cao Yangrong~1 Zhang jinsong~(1***) Chen Shouyi~(1***) (1.National Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,chinese academy of sciences,beijing100101,{2. 2.graduate University of chinese academy of sciences,beijing 100039,{2.) [a]National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China [b]graduate University of chinese academy of sciences Beijing China

Nucleoside diphosphate kinase (NDPK) is a housekeeping enzyme that maintains the intracellular levels of all (d)NTPs used in biosynthesis except *** we report that a full-length cDNA encoding nucleoside diphosphate kinase (NDPK) was cloned us- ing yeast two-hybrid approach.A tobacco NDPK gene was obtained and designated as ***1 is 704 bp in length and en- codes a putative 16.2.kD protein of 148 amino *** analysis showed that NtNDPK1 is highly homologous to other plant NDPK genes and identified as type I (NDPK1).RNA-gel blot analysis showed that there was no significant difference of NtNDPK1 ex- pression in roots,stems,leaves and *** expression of NtNDPK1 was induced by ABA and PEG and repressed by NaCl,but not significantly affected by Paraquat,wounding and low temperature (4℃) treatments,indicating that NtNDPK1 may play a certain role in response to abiotic *** vitro phosphorylation assay demonstrated that NtNDPK1 had autophosphoryLation activity.

关键词： abiotic stress autophosphorylation activity NtNDPK1 tobacco yeast two-hybrid

Discovery of immune related factors in Fenneropenaeus chinensis by annotation of ESTs

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Progress in Natural Science:Materials International 2004年第1期14卷 48-55页

作者： SHEN Yaoqing 1,2. , XIANG Jianhai 1*, WANG Bing 1,2. , LI Fuhua 1 and TONG Wei 3(1. Experimental Marine Biology Laboratory, Institute of Oceanology, chinese academy of sciences, Qingdao 2.6071,china 2. {2. School of the chinese academy of sciences, beijing 100039, china 3. Genomics & Bioinformatics Institute, chinese academy of sciences, beijing 101300, china) chinese Acad Sci Inst Oceanol Expt Marine Biol Lab Qingdao 266071 Peoples R China chinese Acad Sci Grad Sch Beijing 100039 Peoples R China chinese Acad Sci Geonom & Bioinformat Inst Beijing 101300 Peoples R China

A total of 10446 expressed sequence tags (ESTs) are obtained by a large scale sequencing of a cDNA library from cephalothorax of adult Fenneropenaeus chinensis . An EST analysis platform was built up based on local computers and bioinformatic techniques were used to annotate these ESTs in order to promptly find possible functional genes, especially for immune related factors. About 4% of the ESTs show similarity to the coding sequences of such factors, including lectin, serine protease, serpin, lysozyme, etc. These ESTs provide a partial profile of the immune system in F. chinensis and useful information for further study on these genes.

关键词： Fenneropenaeus chinensis bioinformatics ESTs immune factors

Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, chinese academy of sciences, Wuhan 430072. china 2. {2. School of chinese academy of sciences, beijing 100039, china) [a]State key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China China [b]graduate School of chinese academy of sciences Beijing China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4 amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4 bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

Parentage determination of an isolated Yangtze finless porpoise population Neophocaena phocaenoides asiaeorientalis in the Yangtze Tian-e-Zhou Baiji National Natural Reserve based on molecular data

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Progress in Natural Science:Materials International 2005年第2期15卷 54-61页

作者： XIA Junhong 1,2. ZHENG Jinsong 1,2. XU Limei 1,2.and WANG Ding1*(1. Institute of Hydrobiology, chinese academy of sciences, Wuhan 430072. china 2. {2. School of the chinese academy of sciences, beijing 100039, china) Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China. graduate School of the chinese academy of sciences Beijing 100039 China

Reproductive behaviors are poorly known for the Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. In this study, the parentage of an isolated Yangtze finless porpoise population inhabiting the Yangtze Tian-e-Zhou Baiji National Natural Reserve is determined by analysis of microsatellite loci and mitochondrial DNA (mtDNA) control region sequences, and the porpoise's reproductive behaviors are studied. Overall 4 full parentage assignments and additional 3 single parentage assignments were determined for the population of 2. individuals. The analysis shows that their estimated reproductive cycle is shorter than that reported previously and there probably exists an overlapping between gestation and lactation period. The study also shows that the female does not show fidelity to a particular male for breeding and vice versa, the oldest males did not monopolize mating and the dominance rank could not be so strict for the porpoise society. Moreover, the porpoise's mating pattern and relatedness among candidate parents are discussed here. These results provide important information for making guidelines of management and conservation for this protected population.

关键词： Yangtze finless porpoise isolated population reproductive behavior parentage

The deep thermal characteristic of continental margin of the northern South china Sea

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chinese Science Bulletin 2000年第18期45卷 1717-1722页

作者： ZHANG Jian & WANG Jiyang1. graduate School, University of Science and Technology of china, {2., beijing 100039, china 2. Institute of Geology, {2., beijing 10002., china graduate School University of Science and Technology of China Chinese Academy of Sciences Beijing China Institute of Geology Chinese Academy of Sciences Beijing China

Heat flow plays an important role in the study ot thermal structure and thermal evolutionof continental margin of the northern South china Sea. The analysis of heat flow value shows that margin heat flow in the northern South china Sea is relatively high setting, but the percentage of crustal heat flow is lower than 35% in terrestrial heat flow. The terrestrial heat flow exhibited a current of rise from the Northern Continental Margin to the Southern Central Basin. However, the proportion of crustal heat flow in terrestrial heat flow slowly dropped down in the same direction. It is suggested that the main factor causing high heat flow setting is the moving up of hot material from asthenosphere.

关键词： margin of northern South china Sea terrestrial heat flow thermal structures.

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Structures and physical properties of rigid polyurethane foams with water as the sole blowing agent

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Science china Chemistry 2006年第4期49卷 363-370页

作者： LI Xiaobin1,2. CAO Hongbin1 & ZHANG Yi1 1. Institute of Process Engineering, chinese academy of sciences, beijing 100080, {2. 2. graduate School of the chinese academy of sciences, beijing 100039, {2. 1. Institute of Process Engineering Chinese Academy of Sciences Beijing 100080 China 2. graduate School of the chinese academy of sciences Beijing 100039 China

Rigid polyurethane foams blown by varying water level were prepared in this study. The structures and physical properties of rigid polyurethane foams blown by water were measured with FT-IR, universal testing machine (Instron3365), scanning electron microscope (SEM) and differential scanning calorimeter (DSC). The results show that polyurea and polybiuret were the typical charac- teristics, and the cream time and gel time were shorter for the fully water blown rigid foams than that for the fully cyclopentane blown foams. The density of foam samples decreased from 45.0 kg/m3 to 2..4 kg/m3 with the increase of water level from 3 pph to 7 pph. Compressive strength exhibited the similar behavior with density. The average cell size of foam samples ranged from 2.1 μm to 356 μm with the increase of water level from 3 pph to 7 pph, respectively. At the same time, poorer dimen- sional stability was encountered with the increase of average cell size due to fast diffusion rate of CO2.out of the foam. The results of thermal analysis show that the glass transition temperature (Tg) shifted to a higher temperature with the increase of isocyanate level when more water was used as chemical blowing agent.

关键词： polyether polyol, polyurethane rigid foam, compressive strength, dimensional stability, cell size, glass transition temperature.

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A novel operation mode for spaceborne polarimetric SAR

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Science china(Information sciences) 2011年第4期54卷 884-897页

作者： QI WeiKong 1,2.& YU WeiDong 1 1 Institute of Electronics,chinese academy of sciences,beijing 100190,{2. 2.graduate School of chinese academy of sciences,beijing 100039,{2. 1. Institute of Electronics Chinese Academy of Sciences Beijing 100190 China2. Graduate School of Chinese Academy of Sciences Beijing 100039 China

A novel operation mode for spaceborne polarimetric SAR,named polarization space-division adding polarization code-division (PSD-PCD) operation mode,is proposed in this paper through combining polarimetric SAR with elevation digital beam-forming (DBF) on *** a pulse repetition interval the up-chirp LFM sub-pulse is transmitted with horizontal polarization antenna,and with some delay the down-chip LFM subpulse is transmitted with vertical polarization antenna to realize code *** every sub-aperture receives returns from ground,SAR system uses range DBF to separate echoes relative to different sub-pulses in order to realize space *** system PRF is equal to that of single-polarization SAR,and elevation DBF help reduce range ambiguity ***,the main system parameters,including PRF,delay between two sub-pulses,radar equation and ambiguity are analyzed and compared with those of single-polarization *** results demonstrate the feasibility and validity of PSD-PCD operation mode.

关键词： spaceborne synthetic aperture radar (SAR) polarimetry digital beamforming (DBF) operation mode ambiguity ratio polarization space-division polarization code-division

Wind vector retrieval using dual polarization imagery of ASAR

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Progress in Natural Science:Materials International 2006年第11期16卷 1183-1187页

作者： SONG Guiting~ 1,2., HOU Yijun~1** and QI Peng~1(1. Institute of Oceanology, chinese academy of sciences, Qingdao 2.6071, china 2. {2. School, chinese academy of sciences, beijing 100039, china) [a]Institute of Oceanology Chinese Academy of Science Qingdao China [b]graduate School Chinese Academy of Sciences Beijing China

A new algorithm for retrieving wind vector using dual polarization imagery of advanced synthetic aperture radar (ASAR) is developed and tested. Based on the combination of co-polarization and cross-polarization, this new algorithm effectively eliminates the 180° ambiguity which occurs when using single imagery of the ASAR to retrieve wind vector. This algorithm also solves the problem that the retrieval results will break down on very small spatial scales. The results retrieved from dual polarization imagery of ASAR show that the wind speed and direction are in agreement with the data from Quikscat and buoy measurements. The root mean square errors of wind direction and speed between the retrieved results and data from Quikscat are 2.2.° and 0.53 m/s, respectively. Comparisons between the new method and the traditional method show that the new methods fit the buoy data and Quikscat data a little better than the traditional method in this image.

关键词： wind vector retrieval ASAR dual polarization.