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Identification and culture of neural stem cells isolated from adult rat subventricular zone following fluid percussion brain injury

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Journal of Pharmaceutical analysis 2010年第3期22卷 175-178页

作者： Ping-Lin Yang1,Jian-Qiang Qu2.Xi-Jing He1,Hao-Peng Li1 1.the Second Department of Orthopaedics 2.Department of Neurosurgery,the Second Affiliated Hospital,Medical School of Xi’an Jiaotong university,Xi’an 710004,china Second Department of Orthopaedics Second Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710004 China Department of Neurosurgery Second Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710004 China

Objective To analyze proliferation and differentiation of glial fibrillary acid protein(GFAP)-and nestin-positive(GFAP+/nestin+)cells isolated from the subventricular zone following fluid percussion brain injury to determine whether GFAP+/nestin+ cells exhibit characteristics of neural stem *** Male Sprague-Dawley rats,aged 12.weeks and weighing 2.0-2.0 g,were randomly and evenly assigned to normal control group and model *** the model group,a rat model of fluid percussion brain injury was *** days later,subventricular zone tissue was resected from each group and made into single cell *** serum-free neural stem cell medium culture and subsequent serum-induced differentiation,cell type,proliferation and differentiation capacities were determined by immunofluorescence staining and flow *** At 3-7 days after fluid percussion brain injury,nestin+/GFAP+ cells in the single cell suspension from the model group significantly outnumbered those from the normal control group(Pcenter formed after primary culture,and were clearly visible with the increase of culture time and medium *** several passages,many clonal spheres were obtained,suggesting strong self-proliferatiing *** from the model group differentiated into astrocytes,neurons and *** GFAP+/nestin+ cells isolated from the adult rat subventricular zone after fluid percussion brain injury are thought to be neural stem cells because of their self-renewal and multi-differentiation capacities.

关键词： brain injury astrocyte neural stem cell subventricular zone

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Design,Synthesis of Chiral Subunit-containing Polyamides and Their Regulation to Overexpressional PD-1 on HIV-,HBV-and HCV-infected Cells

Design,Synthesis of Chiral Subunit-containing Polyamides and...

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中国化学会第2.届学术年会

作者： Yan-Ling Wu1,*,Dong-Long Xie2.Yan-Ping Ding1,Jian Gao1,Wen Zhang2.* 1Virus Inspection Dept,Zhejiang Provincial center for Disease Control and Prevention,310051,Hangzhou,china 2.ept of Biopharmaceutical Sciences,College of Pharmaceutical Sciences,Zhejiang {2. of Technology,310014,Hangzhou,china

＜正＞The programmed death-1 （PD-1） molecule is an immunoreceptor, which belongs to the CD2. family and is inducibly expressed on activated T cells, NKT cells, B cells, and myeloid cells. Recent studies have shown t... 详细信息

关键词： Polyamide Chirality Regulation PD-1 HIV/HBV/HCV-infected Cells

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基于单视角点云镜像的猪只体尺测量方法

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农业机械学报 2018年第3期49卷 187-195页

作者：王可郭浩马钦苏伟华明睿朱德海中国农业大学信息与电气工程学院北京100083 中国农业大学农业部农业信息获取技术重点实验室北京100083

为了克服人工家畜体尺测量耗时、应激大和工作强度大等问题,提出了一种基于单视角点云镜像的猪只体尺测量方法。首先使用单Xtion深度相机采集包含猪体的场景点云图像序列,并人工筛选出包含背部弯曲程度较小猪体的场景点云图像,然后基于... 详细信息

为了克服人工家畜体尺测量耗时、应激大和工作强度大等问题,提出了一种基于单视角点云镜像的猪只体尺测量方法。首先使用单Xtion深度相机采集包含猪体的场景点云图像序列,并人工筛选出包含背部弯曲程度较小猪体的场景点云图像,然后基于随机采样一致性算法和聚类分割算法自动化分割目标猪体并对其进行姿态归一化,检测单视角猪体点云对称面,并利用对称面镜像获取完整猪体,最后利用自主研发的体尺测量软件测量猪只体尺。试验结果表明,利用该方法测量体长的平均相对误差为5.00%,臀宽测量的平均相对误差为7.40%,臀高测量的平均相对误差为5.74%。该方法为猪只体尺测量提供了切实可行的新途径。

关键词：猪只体尺测量 Xtion深度相机点云镜像

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The susceptibility gene for familial nasopharyngeal carcinoma is mapped on chromosome 4p11-p14 by haplotype analyses

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Chinese Science Bulletin 2003年第21期48卷 2327-2330页

作者： CHEN Hankui, FENG Bingjian, LIANG Hui, ZHANG Ruhua & ZENG Yixin Cancer center, Sun Yet-sun university, Guangzhou 510060,{2. Correspondence should be addressed to Zeng Yixin (e-mail: yxzeng@ ***) Cancer center Sun Yet-sun University China

In our previous study, one candidate suscepti-bility locus for familial nasopharyngeal carcinoma (NPC) has been defined to a 14.2.-cM region on 4p15.1-q12. whereas the distal minimum boundary of this region remained to be further determined in respect that the two markers D4S2.96 and D4S42. were uninformative. In the present study, we carried out a haplotype analysis to identify the exact bound-ary by using the combination of a set of microsatellite mark-ers and single nucleotide polymorphism (SNP) markers in two major NPC families. We defined the exact distal bound-ary between D4S1577 and D4S3347, and consequently shortened the susceptibility locus to an 8.2.-cM segment on 4p11-p14.

关键词：染色体易受性遗传基因鼻咽癌单模标本分析

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Some characteristics of the atmosphere during an adiabatic process

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Progress in Natural Science:Materials International 2006年第6期16卷 644-648页

作者： GAO Li, LI Jianping and REN Hongli (LASG, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 10002., china Graduate School of Chinese Academy of Sciences, Beijing 100049, china National Climate {2., Laboratory of Climate Studies, china Meteorological Administration, Beijing 100081, china) [a]LASG Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China [b]Graduate School of Chinese Academy of Sciences Beijing China [c]National Climate center Laboratory of Climatic Studies China Meterological Administration Beijing China

Some important characteristics of the atmosphere during an adiabatic process are investigated, which include the invariability of atmospheric entropy range and local surface potential temperature, the conservation of the atmospheric mass intervened between any isentropic surface and the ground, and the isentropic surface intersecting with the ground. The analysis shows that the atmospheric reference state (ARS) for investigation on available potential energy (APE) should be defined objectively as the state which could be approached from the existing atmosphere by adiabatic adjustment, and be related to initial atmospheric state before adjustment. For the initial atmosphere state at any time, its corresponding ARS is different from the one at another time. Based on the above-mentioned conclusions, the reference state proposed by Lorenz cannot be obtained physically, so a new conception, the conditional minimum total potential energy, is put forward in order to objectively investigate atmospheric APE.

关键词： available potential energy adiabatic isentropic surface atmospheric reference state conditional minimum total potential energy.

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Recombinant Neural Protein PrP Can Bind with Both Recombinant and Native Apolipoprotein E In Vitro

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 593-601页

作者： Chen GAO~1 Yan-Jun LEI~(1,2. Jun HAN~1 Qi SHI~1 Lan CHEN~(1,3) Yan GUO~(1,4) Yong-Jun GAO~1 Jian-Ming CHEN~1 Hui-Ying JIANG~1 Wei ZHOU~1 Xiao-Ping DONG~1* 1 State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese center for Disease Control and Prevention,Beijing 100052.china 2.School of Medicine,Xi'an Jiaotong {2.,Xi'an 710061,china 3 National Laboratory of Medical Molecular Biology,Institute of Basic Medical Science,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100005,china 4 College of Science and Veterinary Medicine,Northwest Agriculture and Forest {2.,Yangling 712.00,china StateKeyLaboratoryforInfectiousDiseasePreventionandControl NationalInstituteforViralDiseaseControlandPreventionChineseCenterforDiseaseControlandPreventionBeijing100052China SchoolofMedicine Xi'anJiaotongUniversity

The most essential and crucial step during the pathogenesis of transmissible spongiformencephalopathy is the conformational change of cellular prion protein （PrPC） to pathologic isoform (PrPSc).Alot of data revealed that caveolae-like domains （CLDs） in the cell surface were the probable place where theconversion of PrP proteins *** E （ApoE） is an apolipoprotein which is considered toplay an important role in the development of Alzheimer's disease and other neurodegenerative diseases byforming protein complex through binding to the receptor located in the clathrin-coated pits of the cell *** this study,a 914-bp cDNA sequence encoding human ApoE3 was amplified from neuroblastoma cell *** human ApoE isomers were expressed and purified from Escherichia ***-specificantiserum was prepared by immunizing rabbits with the purified ***/His pull-down assay,immunoprecipitation and ELISA revealed that three full-length ApoE isomers interact with the recombinantfull-length PrP protein in *** regions corresponding to protein binding were mapped in the N-terminalsegment of ApoE （amino acid 1-194） and the N-terminal of PrP （amino acid 2.-90）.Moreover,the recombinantPrP showed the ability to form a complex with the native ApoE from liver *** data provided directevidence of molecular interaction between ApoE and *** also supplied scientific clues for assessing thesignificance of CLDs on the surface of cellular membrane in the process of conformational conversion fromPrPCto PrPScand probing into the pathogenesis of transmissible spongiform encephalopathy.

关键词： prion disease PrP apolipoprotein E protein interaction caveolae-like domain

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Triplex operation for portal hypertension with esophageal variceal bleeding:report of 140 cases

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 534-537页

作者： Liu-Shun Feng, Ke Li, Qi-Ping Peng, Xiu-Xian Ma, Yong-Fu Zhao, Pei-Qin Xu and Xiao-Ping Chen Zhengzhou, china Department of General Surgery, First Affiliated Hospi- tal , Zhengzhou university, Zhengzhou 450052., china Hepatic Surgery center, Tongji Hospital, Tongji Medical College, Huazhong university of Science and Technology, Wuhan 430030, china Department of General Surgery First Affiliated Hospital Zhengzhou University Zhengzhou 450052 China.

BACKGROUND:Portal hypertension is a common dis ease. The surgical therapy of this disease focuses on the re sultant upper digestive tract bleeding, which can imperi patients' life directly. This study was to evaluate the effect of triplex operation ( mesocaval C shunt with artificia graft, ligation of the coronary vein and splenic artery) on portal hypertension and its associated upper digestive tract bleeding. METHODS: A retrospective study was made on clinical da- ta of 140 patients undergoing triplex operation, who had suffered from portal hypertension and upper digestive tract bleeding. RESULTS: Postoperative portal pressure was 2.-43 cmH2.O ( preoperative portal pressure 2.-45 cmH2.O ) with the average reduction of 10 cmH2.. One patient (0.7%) died of cerebrovascular disease. Five patients (3.5%) suffered from mild hepatic encephalopathy, which was ameliorated through conservative treatment. Lymphatic fistula occurred in 3 patients (2.1% ) who recovered without treatment 5, 10 days and 3 months after operation respectively. One hundred patients were followed up for 1 month to 6 years without recurrent hemorrhage or hepatic encephalopathy. Hypersplenism and ascites disappeared in 70 patients (70% ) and 80 patients (80% ) respectively. A significant reduction of ascites was seen in 12.patients(12. ). The arti- ficial vessels remained unblocking detected by B type ultra- sonography and Doppler sonography in 95 patients (95% ). CONCLUSION: Triplex operation is suitable for patients with the following portal hypertensions; portal hyperten- sion caused by simple occlusion of the hepatic vein (a patho- logical type of Budd-Chiari syndrome); thrombosis of the portal vein or prehepatic portal hypertension because of cavernous transformation; intrahepatic portal hypertension with rebleeding after splenectomy or non-operation, and those patients with liver function in grade A or B according to the Child-Pugh classification.

关键词： hypertension portal vein esophageal and gastric varices hemorrhage gastrointestinal

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Vacuum Kinematical Sealing for Vacuum Electro-slag Furnace

Vacuum Kinematical Sealing for Vacuum Electro-slag Furnace

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真空与表面工程——第十届国际真空冶金与表面工程学术会议、2.11年真空工程学术会议、2.11年真空咨询工作会议

作者： HAN Jin~1 ZHANG Shi-wei~(1,*) XU Cheng-hai~1 LIU Xihai~2.(1 School of Mechanical Engineering and Automation,Northeastern university,Shenyang 110004,china 2.Large Scale Electro-slag Furnace R&D {2.,NEU Science & Technology Group,Shenyang 110004,china)

Vacuum electro-slag furnace has its unique advantages and ***,one of the key technologies to be resolved in designing of vacuum electro-slag furnace is the vacuum kinematical sealing between the false electrode(stinger) and the vacuum ***,in this paper,characteristics of vacuum electro-slag furnace and four structure types of vacuum kinematical sealing with their advantages and disadvantages are introduced.

关键词： vacuum electro-slag furnace vacuum kinematical sealing

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The　Influence　of　Changes　in　Vegetation　Type　on　the　Surface　Energy　Budget

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Advances in Atmospheric Sciences 1994年第2期11卷 139-161页

作者： Runhua Yang (1) J. Shukla (1) P. J. Sellers (2. 1. center for Ocean-Land-Atmosphere Studies 4041 Powder Mill Road 20705 Calverton MD USA 2. NASA / GSFC 92. 20771 Greenbelt MD USA

The influence of changes in vegetation type on the surface energy budget was studied using the Simple Biosphere Model (SiB) of Sellers et al. (1986). The modeled energy budget response to the conversion of forest to short vegetation or bare soil (deforestation) was investigated with SiB forced by three time--series of atmospheric boundary conditions collected at three different climatic sites: an Amazonian tropical forest, a U.S. Great Plains grassland, and a central Wales spruce forest. The results show that SiB can simulate realistic surface energy budgets and surface temperatures, and that deforestation may have a significant influellce on the local surface energy budget and surface weather. The influence is especially prominent at the Amazonian and U.S. Great Plains sites, and greater in summer than in other *** was found that atmospheric boundary conditions play a dominant role in determining the degree of changes in the surface fluxes and temperature induced by deforestation; the largest change in latent heat flux appeared at the Amazon site, the largest change in sensible heat flux appeared at the Spruce forest site, and the largest change in surface temperature appeared at the Great Plains site. The Bowen ratios of the SiB sensitivity integrations for each site are comparable with observations. The values of the Bowen ratio and the ratio of latent heat flux to net radiation vary distinctly from site to site, implying that local atmospheric conditions limit the range of changes caused by the vegetation change.

关键词： Biosphere model Vegetation effect Sensitivity test Land -atmosphere interaction

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Precise recognition of warhead and decoy based on components of micro-Doppler frequency curves

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Science china(Information Sciences) 2012年第4期55卷 850-856页

作者： GUO KunYi & SHENG XinQing center for Electromagnetic Simulation,School of Information and Electronics,Beijing Institute of Technology,Beijing 100081,china 1. center for Electromagnetic Simulation School of Information and Electronics Beijing Institute of Technology Beijing 100081 China

From the view of electromagnetic scattering,it is indicated that the micro-Doppler (m-D) character-istics of an extended target undergoing micro-motions are actually induced by the change of incident directions of radar *** micro-motions may lead to similar change of incident directions,consequently inducing similar m-D *** tackle this problem,rather than distinguish warhead and decoy directly from m-D characteristics,the frequency components of m-D frequency curves are used as a new characteristic for recognition in this *** get high precision of frequency components estimation,model-based parameter estimation (MBPE) is utilized to extract the m-D frequency curves from *** obtain high accurate simu-lation results,the backscattered signal simulation is conducted by full-wave numerical *** simulation results validate the theoretical analysis and the high performance of the proposed method.

关键词： micro-Doppler(m-D) target recognition time-frequency representation (TFR) model-based pa-rameter estimation (MBPE)

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