Identification and culture of neural stem cells isolated from adult rat subventricular zone following fluid percussion brain injury

作     者：Ping-Lin Yang1,Jian-Qiang Qu2,Xi-Jing He1,Hao-Peng Li1 1.the Second Department of Orthopaedics 2.Department of Neurosurgery,the Second Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710004,China 

作者机构：Second Department of Orthopaedics Second Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710004 China Department of Neurosurgery Second Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710004 China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2010年第22卷第3期

页      面：175-178页

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.30371442) New Teachers Foundation of Ministry of Education(No.20070698073) 

主　　题：brain injury astrocyte neural stem cell subventricular zone 

摘      要：Objective To analyze proliferation and differentiation of glial fibrillary acid protein(GFAP)-and nestin-positive(GFAP+/nestin+)cells isolated from the subventricular zone following fluid percussion brain injury to determine whether GFAP+/nestin+ cells exhibit characteristics of neural stem *** Male Sprague-Dawley rats,aged 12 weeks and weighing 200-250 g,were randomly and evenly assigned to normal control group and model *** the model group,a rat model of fluid percussion brain injury was *** days later,subventricular zone tissue was resected from each group and made into single cell *** serum-free neural stem cell medium culture and subsequent serum-induced differentiation,cell type,proliferation and differentiation capacities were determined by immunofluorescence staining and flow *** At 3-7 days after fluid percussion brain injury,nestin+/GFAP+ cells in the single cell suspension from the model group significantly outnumbered those from the normal control group(P0.01).In the model group,an increased number of small neurospheres with smooth cell edge and bulged center formed after primary culture,and were clearly visible with the increase of culture time and medium *** several passages,many clonal spheres were obtained,suggesting strong self-proliferatiing *** from the model group differentiated into astrocytes,neurons and *** GFAP+/nestin+ cells isolated from the adult rat subventricular zone after fluid percussion brain injury are thought to be neural stem cells because of their self-renewal and multi-differentiation capacities.