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Molecular dynamics simulations of interaction between protein-tyrosine phosphatase 1B and a bidentate inhibitor

Acta Pharmacologica Sinica 2006年第1期27卷 100-110页

作者： Gui-xia LIU~(2.3,4) Jin-zhi TAN~2.Chun-ying NIU~2.Jian-hua SHEN~(2.3) Xiao-min LUO~2.Xu SHEN~(2.3) Kai-xian CHEN~2.Hua-liang JIANG~(2.3,4)2.center for Drug Discovery and Design,State Key Laboratory of Drug research,{2. Institute of Materia Medica,shanghai institutes for biological sciences,chinese academy of sciences,{2. 2.12.3,China 3 School of Pharmacy,East China University of Science and Technology {2. 2.02.7,China centerforDrugDiscoveryandDesign StateKeyLaboratoryofDrugResearchShanghaiInstituteofMateriaMedicaShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai201203China

Aim:To investigate the dynamic properties of protein-tyrosine phosphatase (PTP)1B and reveal the structural factors responsible for the high inhibitory potencyand selectivity of the inhibitor SNA for ***:We performed moleculardynamics (MD) simulations using a long time-scale for both PTP1B and PTP1Bcomplexed with the inhibitor SNA,the most potent and selective PTP1B inhibitorreported to *** trajectories were analyzed by using principal ***:Trajectory analyses showed that upon binding the ligand,theflexibility of the entire PTP1B molecule *** most notable change is themovement of the *** simulation results also indicated that electro-static interactions contribute more to PTP 1B-SNA complex conformation than thevan der Waals interactions,and that Lys41,Arg47,and Asp48 play important rolesin determining the conformation of the inhibitor SNA and in the potency andselectivity of the *** these,Arg47 contributed *** results werein agreement with previous experimental ***:The informationpresented here suggests that potent and selective PTP1B inhibitors can be de-signed by targeting the surface residues,for example the region containing Lys41,Arg47,and Asp48,instead of the second phosphate binding site (besides theactive phosphate binding site).

关键词： molecular dynamics simulation principal component analysis protein-tyrosine phosphatase 1B type 2.diabetes

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Functional study of MeCBF1 transcription factor from cassava

Functional study of MeCBF1 transcription factor from cassava

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2.11全国植物生物学研讨会

作者： Dong An~1,Peng Zhang~(1,2.) 1 National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology, shanghai institutes for biological sciences,chinese academy of sciences,300 Fenglin Road, {2. 2.0032.China 2.{2. Chenshan Plant Science research center,chinese academy of sciences,{2. Chenshan Botanical Garden,3888 Chenhua Road,{2. 2.1602.China

DREB 1/C-repeat binding factor(CBF) is a plant-specific family of transcription factors that play a crucial role in freezing *** order to study the abiotic stress tolerance in cassava(Manihot esculenta Crantz),a DREB1A/CBF-like gene, designated as MeCBF1,was ***1 contains all the conserved domains known to already identified CBF proteins of other plant species,indicating that this protein could be classified as a typical member of the DREB ***

关键词： cassava CBF transcription factor cold stress tolerance

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Development of Transgenic Sweet Potato(Ipomoea batatas) Plants Tolerant to Salt Stress

Development of Transgenic Sweet Potato(Ipomoea batatas) Plan...

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植物分子生物学与现代农业——2.10全国植物生物学研讨会

作者： Weijuan Fan and Peng Zhang~* shanghai center for Cassava {2.,Institute of Plant Physiology & Ecology,shanghai institutes for biological sciences,chinese academy of sciences,300 Fenglin Road,shanghai 2.0032.China

Sweet potato is a herbaceous perennial root crop and provides major feedstock for food and biofuel industries in *** stresses,such as salinity,drought and low temperature,are critical delimiters for high productivity ... 详细信息

关键词： sweet potato over-expression Na+/H+ antiporter betaine aldehyde dehydrogenase

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Identification and classification of all potential hemolysin encoding genes and their products from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai

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Acta Pharmacologica Sinica 2005年第4期26卷 453-461页

作者： Yi-xuan ZHANG~(2.3,4,5) Yan GENG(2.3,4,5) Bo BI~(2.4) Jian-yong HE~4 Chun-fu WU~4 Xiao-kui GUO~3 Guo-ping ZHAO~(2.4,6,7) 2.research center of biotechnology,shanghai institutes for biological sciences,{2.,shanghai 2.02.3,China 3 Department of Microbiology and Parasitology,shanghai Second Medical University,shanghai 2.002.,China 4 The School of Pharmaceutical Engineering,Shenyang Pharmaceutical University,Shenyang 110016,China 6 chinese National Human Genome center at shanghai,ZhangJiang High Tech Park,shanghai 2.12.3,China researchcenterof{2. ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200233

Aim:To identify and classify all potential hemolysin candidates of Leptospirainterrogans serogroup Icterohaemorrhagiae serovar ***:All of thepotential hemolysin encoding genes were characterized in *** geneswere cloned and expressed in Escherichia *** hemolytic activities of theexpressed proteins were assayed observing the hemolysis on sheep blood *** activities of the hemolysin candidates were measuredby thin-layer chromatography(TLC)and HPLC for *** and secretion of the hemolysins in L interrogans were studied byreverse transcription polymerase chain reaction,Western blot,and enzyme-linkedimmunosorbent *** and Conclusion:The hemolytic activities ofhemolysin candidates(LA032.,LA0378,LA102.,LA102.,LAI650,LA3050,LA3937,LA4004)from L interrogans strain Lai were *** were furtherdivided into two groups,sphingomyelinase hemolysins and non-sphingomyelinasehemolysins,based on their ability to hydrolyze *** of thesehemolysins were actually expressed in living L interrogans and some of themwere secreted into the *** study establishes an essential findcomplete basis for further studying the contribution of hemolysins to the patho-genesis of L interrogans.

关键词： hemolysin Leptospira interrogans sphingo myelinase

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细菌对氨基糖苷类抗生素产生抗性的机理及其控制策略

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中国生物工程杂志 2004年第11期24卷 9-12页

作者：周勤华张毅中国科学院上海生命科学研究院生物工程研究中心上海200233

氨基糖苷类抗生素在治疗感染性疾病尤其是革兰氏阴性菌引起的严重感染方面起着重要作用 ,但是耐药菌株的出现较大地限制了此类抗生素的发展 ,因此 ,如何控制耐药性已经成为一项迫切需要解决的任务。细菌对氨基糖苷类抗生素产生抗性的机... 详细信息

氨基糖苷类抗生素在治疗感染性疾病尤其是革兰氏阴性菌引起的严重感染方面起着重要作用 ,但是耐药菌株的出现较大地限制了此类抗生素的发展 ,因此 ,如何控制耐药性已经成为一项迫切需要解决的任务。细菌对氨基糖苷类抗生素产生抗性的机制很多 ,目前普遍接受的主要有三种 :1 通过减少对氨基糖苷类抗生素的摄取或减少药物在体内的累积而产生抗性。 2. 通过改变核糖体结合位点而产生抗性。 3 通过表达氨基糖苷类抗生素修饰酶而产生抗性。目前细菌耐药性的控制主要集中在对原有氨基糖苷类抗生素进行改造或合成新的抗生素 ,开发氨基糖苷类抗生素修饰酶抑制剂。

关键词：氨基糖苷类抗生素治疗修饰酶细菌过表达严重感染体内抗性累积耐药菌株

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Large-scale genome sequencing based genotyping and genome-wide association studies in rice

Large-scale genome sequencing based genotyping and genome-wi...

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第十一届全国植物基因组学大会

作者： Bin Han National center for Gene research & Institute of Plant Physiology and Ecology,{2. institutes of biological sciences,chinese academy of sciences Beijing Institute of Genomics,CAS

Rice(Oryza sativa L.) is the staple food for more than half of the world *** thousands of years of rice cultivation,China has accumulated an enormously diverse collection of landraces. This is an invaluable genetic re...

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Genome-wide association study of complex traits in rice

Genome-wide association study of complex traits in rice

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第十三届全国植物基因组学大会

作者： Xuehui Huang~(1,2.,Bin Han~(1*) 1 National center for Gene research,Institute of Plant Physiology and Ecology,{2.,chinese academy of sciences,shanghai 2.02.3,China 2.CAS Key Laboratory of Genome sciences and Information,Beijing Institute of Genomics, chinese academy of sciences,Beijing 10002.,China

Rice(Oryza sativa L.) is the staple food for more than half of the world population. After thousands of years of cultivation,rice landraces have accumulated an enormous of important agronomic traits,which are invaluable genetic resources for breeding elite varieties for sustainable ***,rice has a high-quality reference genome sequence and the self-fertilization system that allows simplified haplotype identification and repeated phenotyping for *** direct

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Cloning and characterization of a vacuolar cation/proton antiporter in sweet potato（Ipomoea batatas）

Cloning and characterization of a vacuolar cation/proton ant...

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第六届中国植物逆境生理学与分子生物学学术研讨会

作者： Weijuan Fan and Peng Zhang SIBS-ETH shanghai center for Cassava {2.,Institute of Plant Physiology & Ecology,shanghai institutes for biological sciences,chinese academy of sciences,300 Fenglin Road,shanghai 2.0032.China

＜正＞Sweet potato is a herbaceous perennial root crop and has been considered as an important source of food products and biofuels in China because of its stable yield and high tolerance to drought,salinity and infertile *** increase our understanding of the mechanism of salinity resistance in sweet potato,a vacuole Na+/H+antiporter gene,named IbNHX2.was identified by differential screening of a sweet potato cDNA *** IbNHX2.DNA is2.58b

关键词： sweet potato Na+/H+ antiporter gene salt resistance functional analysis

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Regulated expression of TATA-binding protein-related factor 3(TRF3)during early embryogenesis

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Cell research 2006年第07期16卷 8+611-621页

作者： Ye Yang~1 Jian Cao~1 Lu Huang~1 Hai Yan Fang~2.Hui Zhen Sheng~(1,2. ~1Laboratory of Stem Cell Biology,Institute of Biochemistry and Cell Biology,shanghai institutes.for {2. sciences,chinese academy of sciences,shanghai 2.0031 China ~2.6} for Developmental Biology,Xinhua Hospital,School of Medicine,shanghai Jiao Tong University,1665 Kong Jiang Road,shanghai 2.0092.China LaboratoryofStemCellBiology InstituteofBiochemistryandCellBiologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China centerforDevelopmentalBiology XinhuaHospitalSchoolofMedicine

RNA polymerase（Pol）Ⅱ transcription persists in TATA-box-binding protein（TBP）-/-mutant mouse embryos,indicat-ing TBP-independent mechanisms for Pol Ⅱ transcription in early ***-related factor3（TRF3）has beenproposed to substitute for TBP in TBP-/-mouse *** examined the expression of TRF3 in maturing oocytes andearly embryos and found that TRF3 was co-expressed with TBP in the meiotic oocytes and early embryos from the lateone-cell stage *** amounts of TBP and TRF3 changed dynamically and correlated well with *** immunoprecipitation（CHIP）assay revealed that different gene promoters in mouse embryonic stem（ES）cells recruited TRF3 and TBP *** analyses of TRF3 and TBP during cell cycle showed thatboth factors proceeded through cell cycle in a similar pace,except that TRF3 was slightly delayed than TBP in enteringthe nucleus when cells were exiting the *** from expression and biochemical analyses therefore support thehypothesis that TRF3 plays a role in early mouse *** addition,results from co-localization study suggestthat TRF3 may be also involved in Pol I transcription.

关键词： TBP TRF3 oocyte embryo ES cell NIH3T3 cell

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Enhanced Anthocyanin Production by Systemic Up-Regulation of Anthocyanin Biosynthetic genes in Sweet Potato(Ipomoea batatas)

Enhanced Anthocyanin Production by Systemic Up-Regulation of...

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植物分子生物学与现代农业——2.10全国植物生物学研讨会

作者： Min Zhang~1,Ling Yuan~2.Peng Zhang~(1,3*) 1 shanghai center for Cassava {2.,Institute of Plant Physiology & Ecology,shanghai institutes for biological sciences,chinese academy of sciences,300 Fenglin Road,shanghai 2.0032.China 2.Department of Plant and Soil sciences,University of Kentucky,Lexington,KY 40546,USA 3 Chenshan Plant Science research center,chinese academy of sciences,Chenshan Botanical Garden,3888 Chenhua Road,shanghai 2.1602.China

Anthocyanins are catalyzed by a series of enzymes in *** regulatory genes (*** factors) could regulate the biosynthetic pathway of anthocyanins in *** increase anthocyanin accumulation in sweet potato,over-expression of the maize(Zea mays L.) leaf colour(Lc) transcription factor in transgenic sweet potato was *** transgenic plants were regenerated from transformed calli of cultivar

关键词： Sweet potato anthocyanin biosynthesis transcription factor Lc systemic regulation

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