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上海实验动物科学 2002年第3期22卷 133-137页

作者：金玫蕾郭宁李晨丁澄谢青莲邹洪王凌燕于军景乃禾中国科学院上海生命科学研究院生物工程研究中心上海200233 中国科学院上海生命科学研究院生物化学与细胞生物学研究所上海200031

作者利用反义核酸技术建立了用于神经系统新基因功能筛选研究用的小鼠行为学检测平台 ,用 BALB/ c小鼠对 38个小鼠的新基因功能进行了初步筛选 ,又从中选取了 10个与生理盐水 (saline)及随机序列核酸 (scramble)两个对照组都具有统计学... 详细信息

作者利用反义核酸技术建立了用于神经系统新基因功能筛选研究用的小鼠行为学检测平台 ,用 BALB/ c小鼠对 38个小鼠的新基因功能进行了初步筛选 ,又从中选取了 10个与生理盐水 (saline)及随机序列核酸 (scramble)两个对照组都具有统计学差异的基因 ,进一步使用 C5 7BL / 6和 ICR小鼠进行了复筛。实验结果发现 ,各品系小鼠的表现不尽相同。初筛结果显示 :10个基因中与记忆相关的有 6个 ,与痛觉相关的有 2. ,另 2.与运动相关。但复筛的实验结果为 :与记忆相关的只有 3个 (Am,L和 Sn) ,与痛觉相关的有 2. (Pe和Rp)。研究说明了用不同品系进行复筛的重要性 ,也说明了在基因功能筛选实验中 ,使用近交系实验小鼠优于远交群。研究不同品系在同一实验中的不同表现 ,同研究用不同品系来验证同一实验结果一样。

关键词：实验小鼠品系基因功能反义核酸行为学

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ATPID:an integrative platform for plant systems biology

ATPID:an integrative platform for plant systems biology

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第二届植物代谢国际会议

作者： Peng Li Weidong Zang Tieliu Shi center for Bioinformatics and Computational Biology and The Institute of Biomedical sciences School of Life Science East China Normal University 500 Dong chuan Road {2. 2.02.1 center for Bioinformatics and Computational Biology and The Institute of Biomedical Sciences Schoolof Life Science East China Normal University 500 Dong chuan Road Shanghai 200241 Center for Bioinformatics and Computational Biology and The Institute of Biomedical Sciences S

＜正＞Protein interactions are involved in important cellular functions and biological processes that are the fundamentals of all life activities. With improvements in experimental techniques and progress in research,

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GRK inhibits cell apoptosis and G1 arrest in osteosarcoma cells

GRK inhibits cell apoptosis and G1 arrest in osteosarcoma ce...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Xiao-Qing CHEN,Man YUAN,Hui-Lin ZHU,Lan MA~* Pharmacology research center,Fudan University,{2. 2.0032.China

G-protein-coupled receptor(GPCR) kinases(GRKs) have been demonstrated to phosphorylate and sequester a variety of *** of individual GRK subtype in mice causes dysfunction in cardiovascular,nervous,respiratory, optical and immune systems,or even embryonic *** addition to their well-established roles,emerging evidence has revealed novel cellular functions of *** are also reported to phosphorylate and regulate non-GPCR receptor including EGFR,PDGFR and Smoothened(Smo).Furthermore,an increasing number of non-receptor substrates of GRKs have been identified such as epithelial Na channels,synuclein,tubulin,ribosomal protein P2.and *** findings indicate additional and important cellular mechanisms of GRKs other than attenuating GPCR ***,the functional effects of many of these non-GPCR phosphorylation events still remain *** the present study,we revealed a novel function for GRK in tumorigenesis,and thus effect cell cycle and apoptosis in response to DNA damage.

关键词： G protein-coupled receptor kinase apoptosis cell cycle arrest phosphorylation

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HIF-1 can protect neprilysin from hypoxia impairment

HIF-1 can protect neprilysin from hypoxia impairment

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Miao SUN~1,Ling-Ling ZHU~2.Ming FAN~2.De-Hua CUI~(1,*) 1 Neuroscience research Institute and Department of Neurobiology,Key Laboratory for Neuroscience,Ministry of Education,Key Laboratory for Neuroscience,Ministry of Public Health,Peking University Health Science center, Beijing 100191,China 2.Department of Brain Protection and Plasticity,Institute of Basic Medical {2.,Beijing,100850,China

＜正＞Objective Pathogenesis of Alzheimer’s disease（AD）,which is characterized by accumulation of extracellular deposits of P-amyloid peptide（Abeta） in the brain,has recently been linked to vascular disorders such as hypoxia. Extracellular deposits of P-amyloid peptide are closely associated with the metabolic balance between Abeta production and clearance *** recent work reports that hypoxia will disturb this metabolic balance by increasing Abeta production,little is known about hypoxia and Abeta *** neprilysin（NEP） is a mainly Abeta degradation enzyme,we focused on the effect of hypoxia on *** The changes of neprilysin expression level under hypoxia condition are mainly conduct in SH-SY5Y cell line by real-time PCR,western blot and *** confirm relationship between HIF and neprilysin,HIF-1αplasmid and shRNAi are ***（1） Severe hypoxia can decrease neprilysin protein and enzyme activity immediately in SH-SY5Y cells（2. the endogenous HIF can transiently attenuate this effect.（3） Over-express HIF-1αcan increase neprilysin expression level under hypoxia condition.（4） HIF-1 can directly regulate neprilysin protein and activity *** HIF-1 may protect neprilysin from hypoxia impairment by directly regulation.

关键词： hypoxia neprilysin(NEP) Hypoxia Inducible Factor-1α(HIF-1α)

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A pilot clinical study:tracking neural stem cells after implantaion in brain trauma patients

A pilot clinical study:tracking neural stem cells after impl...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Jian-Hong ZHU,Liang-Fu ZHOU,Li PAN,Liang GAO,Wei-Ming BAO,Xing WU,Xue-Hai WU,Xiao-Mei WANG,LuPing CHEN,Yi-Wen SHEN,Qi-Shen TANG National Key Laboratory for Medical Neurobiology,institutes for Brain Science research,Fudan University {2. Medical College,Departments of Neurosurgery,Fudan University Huashan Hospital,{2. Neurosurgical Emergence center,{2. 2.0040,China

＜正＞Regeneration of damaged brain tissue with neural stem cells（NSCs） is a promising strategy for reversing neurological *** nanoparticles have been used to label and track stem cells in animal experiments. Here,we report the feasibility of labeling human NSCs with Superparamagnetic iron oxide nanoparticles（SPIOs） and tracking them with magnetic resonance imaging（MRI）.We isolated and cultured NSCs from exposed brain tissue of patients with open brain *** an emergency operation,exposed neural tissue from his brain was collected and cultured in a medium previously shown to select neural stem *** day before implantation,we incubated the cells with Feridex IV（a SPIO contrast agent approved by the US FDA5） and lipofection reagent in medium for 60 min to allow the Feridex IV to infuse into the NSCs and thereby label *** were then autologously implanted stereotactically around the region of brain *** was obtained by gradient reflection echo with TR/TE 2.0 ms/2. ms and 2.°flip-angle with an MR imager（Signa3.0T-GE） at 2. hours and every 7 days following transplantation for ten *** observed a susceptibility change with powerful signal damping on T2.weighted *** pronounced hypointense signals were found in the injection sites before *** injection sites were visible as circular dark tissue areas on the first day after *** hypointense signal at each injection point faded *** week after implantation,the change in signal was consistent with the cells accumulating and proliferating around the *** signal around the periphery of the lesion intensified during the second and third weeks,suggesting that the NSCs migrated from the primary sites of injection and to the border zone of the damaged *** did not observe hypointensive signal after seven weeks,which we attribute to a dilution of signal by cell *** explore the possibility that magnetic signal was generated

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Electroacupuncture downregulates TLR2.4 and pro-inflammatory cytokine expression after surgical trauma stress without adrenal glands involvement

Electroacupuncture downregulates TLR2/4 and pro-inflammatory...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Jun WANG,Jing LI,Yan-Qing WANG,Gen-Cheng WU~* Department of Integrative Medicine and Neurobiology,Institute of Acupuncture research WHO Collaborating center for Traditional Medicine,{2. of Brain Science,State Key Laboratory of Medical Neurobiology,Fudan University, shanghai,2.0032.China

＜正＞Cumulative evidences suggest that electroacupuncture（EA） can modulate immune function,but the mechanism needs further *** the present study,the contribution of EA on toll-like receptor 2.and 4（TLR2.TLR4） and proinflammatory cytokine expression after surgical trauma stress were *** mRNA level of both TLR2.4 and proinflammatory cytokine was measured by quantitative real-time *** and western blot assay were chosen for TLR2. TLR4 protein expression and pro-inflammatory cytokine production *** results showed that surgical trauma stress increased TLR2.mRNA and TLR2.4 proteins in the spleen and augmented pro-inflammatory cytokines（***-1β） mRNA and protein expression in the spleen and *** effects could be deteriorated by adrenalectomy（ADX）.EA at "Zusanli" acupoint significantly inhibited surgical trauma induced TLR2.mRNA and TLR2.4 protein expression in spleen and pro-inflammatory cytokine expression in the spleen and ***,however,could not block the effect of *** results suggested that surgical trauma stress primes the innate immune system for enhanced TLR2.expression and pro-inflammatory cytokine *** inhibits TLR2.4 and pro-inflammatory cytokines to produce an anti-inflammatory effect in a surgical trauma stress model,without adrenal gland involvement.

关键词： electroacupuncture surgical trauma stress TLR pro-inflammatory cytokine adrenalectomy

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新疆维吾尔族男性三维人脸图像的年龄估计与年龄面貌重构

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法医学杂志 2018年第4期34卷 363-369页

作者：潘思宇陈诗婷唐鲲李彩霞刘京叶健赵雯婷中国人民公安大学公安部物证鉴定中心北京市现场物证检验工程技术研究中心现场物证溯源技术国家工程实验室中国科学院上海生命科学研究院

目的基于新疆维吾尔族男性三维脸部图像寻找与年龄相关的脸部特征,构建年龄估计模型,重塑个体衰老及年轻时的脸部图像。方法使用Artec Studio软件对采集的105例17~57岁新疆维吾尔族男性人脸三维图像预处理。用Face Analysis软件将人脸... 详细信息

目的基于新疆维吾尔族男性三维脸部图像寻找与年龄相关的脸部特征,构建年龄估计模型,重塑个体衰老及年轻时的脸部图像。方法使用Artec Studio软件对采集的105例17~57岁新疆维吾尔族男性人脸三维图像预处理。用Face Analysis软件将人脸图像转为高密度三维点阵数据,使每张图像可用32.2.1个点所表示。利用广义普鲁克分析法对人脸图像进行中心化校正并统一至同一坐标系。采用偏最小二乘回归法建立年龄估计模型。将年龄相关脸部形态特征的变化展现在平均脸的热图上,并基于该模型进行不同年龄的脸部图像重构。结果随着年龄的增长,平均脸会发生鼻唇沟加深、脸颊凹陷、颧骨突出、眼角下垂等变化。估计年龄和真实年龄之间的Pearson相关系数为0.71,估计年龄的平均绝对偏差(mean absolute deviation,MAD)为6.37岁,>30~40岁组的年龄估计结果最准确(MAD值为4.2.岁),40岁以后偏差随年龄上升。合成的脸部图像随年龄增长脸部形态变化和衰老效果明显。结论本研究在维吾尔族人群中揭示了年龄相关的脸部特征和衰老标记,建立了可靠的年龄估计模型。

关键词：法医人类学成像,三维面部年龄测定年龄面貌合成新疆维吾尔族

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The effects of 2.z electroacupuncture on regional cerebral biodistribution of 11C-carfentanil in rats and the pilot study of small animal PET using this tracer

The effects of 2Hz electroacupuncture on regional cerebral b...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Xiao-Hui XIANG~1 Jin-Ming ZHANG~2.Jia-He TIAN~2.Ji-Sheng HAN~1 Cai-Lian CUI~(1 *) 1 Neuroscience research Institute and Department of Neurobiology Peking University Health Science center Key Laboratory of Neuroscience the Ministry of Education and Ministry of Public Health Beijing 100191 China 2.Department of Nuclear Medicine the {2. PLA General Hospital Beijing 100853 China

＜正＞Objective A deal of evidence shows that 2.Hz electroacupuncture（EA） produces a significant increase in the content of enkephalin and endorphin of CNS in mediating *** emission tomography（PET） is an only real time method to studyμ-opioid receptors in vivo at *** purpose of this study is to investigate the effects of 2.Hz EA on regional cerebral biodistribution of 11C-carfentanil in rats and to study whether 11C-carfentanil can be used as a tracer in small animal PET *** Biodistribution studies performed after intravenous 11C-carfentanil injection by weighing and radioactivity counting the samples of eight brain regions（cerebellum,hypothalamus,thalamus, hippocampus,striatum,cortex,midbrain,pons） in *** biodistribution results of control groups at the time point of 0 min,10 min,2. min,30 min and 60min and EA group at 30 min were presented as regional cerebral tissue/cerebellum radioactivity *** were scanned with small animal PET in list mode and the images of corresponding time points were reconstructed in 3D OSEM *** PET results were analyzed using region of interesting（ROI）/ cerebellum radioactivity *** Regional cerebral biodistribution in the rats showed a significantly higher uptake of 11C-carfentanil by the thalamus,hypothalamus and striatum than by the cerebellum,with hippocampus,cortex and midbrain showing middle *** regional cerebral tissue/cerebellum radioactivity ratio reached the peak at the time point of 30 *** with naloxone significantly reduced the uptake of 11C-carfentanil in the regions aforementioned.2.Hz EA significantly decreased tissue/cerebellum radioactivity ratio of hypothalamic nucleus（P = 0.031）.The findings from small animal PET were consistent with the *** These results indicate that 2.Hz EA for 30 min can increase the content of enkephalin and endorphin in hypothalamus and suggest that 11C-carfentanil may be used as a tracer in res

关键词： μ-opioid receptor 11C-carfentanil regional cerebral biodistribution small animal PET electroacupuncture

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Orexins excite cerebellar interpositus nuclear neurons in rats via orexin 2.receptors in vitro

Orexins excite cerebellar interpositus nuclear neurons in ra...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Lei YU,Xiao-Yang ZHANG,Jun ZHANG,Jing-Ning ZHU*,Jian-Jun WANG~* Department of biological Science and Technology and State Key Laboratory of Pharmaceutical biotechnology,School of Life {2.,Nanjing University,Nanjing 2.0093,China

Orexin A and orexin B(also called hypocretin-1 and -2.,both derived from the common precursor prepro-orexin, are synthesized exclusively within the central nervous *** the cell bodies of neurons producing orexins are narrowly concentrated in the lateral hypothalamus/perifornical region,the orexinergic fibers widely project to various regions in the brain including the cerebellum,an important subcortical center for motor ***,orexins were considered to be neurotransmitters that centrally stimulate food intake,but it soon became evident that orexins are involved in many basic physiological processes including energy homeostasis,sleep-awake cycle,nociception,food and drug addiction,as well as ***,little is known about roles of orexins in central motor ***,the present study was designed to investigate the effect of orexins on neuronal activity of the cerebellar interpositus nucleus (IN),one of the final outputs of the *** this study,western blot and immunofluorescence histochemical results both showed that orexin 1 receptors(OXR) and orexin 2.receptors(OXR) were present in the rat cerebellar IN. Using brain slice preparations and extracellular recordings,186 IN neurons with tonic discharges were recorded from 12. cerebellar *** slices with orexin A(100 nM-1μM) or orexin B(100 nM-1μM) both produced 96 recorded cerebellar IN neurons(96/186,51.6%) a concentration-dependent excitatory *** orexins-induced excitation was not blocked by the low-Ca/high-Mg medium(n = 8),supporting a direct postsynaptic action of the *** OXR antagonist SB-334867(10-30μM) did not block the excitatory response of cerebellar IN neurons to orexins(n = 2.), but[Ala,D-Leu]orexin B(300 nM-1μM),a highly selective OXR agonist,mimicked the excitatory effect of orexins on the cerebellar IN neurons(n = 18).These results demonstrate that orexins excite the cerebellar EN neurons through OXR, although both

关键词： cerebellar interpositus nucleus orexin A orexin B orexinl receptors orexin 2.receptors motor control

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Analysis of SCA2.and SCA3/MJD repeats in Parkinson's disease in China's mainland,genetic,clinical,and PET findings

Analysis of SCA2 and SCA3/MJD repeats in Parkinson's disease...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Jun-Ling WANG~(1,2.,Bin XIAO~(1,2.,Xiang-Xiang CUI~(1,2.,Ji-Feng GUO~(1,2.,Li-Fang LEI~(1,2.,Xing-Wang SONG~(1,2.,Lu SHEN~(1,2., Hong JIANG~(1,2.,Xin-Xiang YAN~(1,2.,Qian PAN~3,Zhi-Gao LONG~3,Kun XIA~3,Bei-Sha TANG~(1,2.3,*) 1 Department of Neurology,Xiangya Hospital 2.Neurodegenerative Disorders research center,Central South University 3 National Lab of Medical Genetics of China,Changsha,410008,China

Background Recent reports suggest that CAG triplet expansions of spinocerebellar ataxia type 2.and 3(SCA2.and SCA3/MJD) genes(ATXN2.and MJD1) are the cause of typical levodopa-responsive Parkinson’s disease(PD), several of which were found in patients of chinese *** is unclear whether ethnicity(or founder effects within certain geographical region) alone can explain such an *** To investigate the prevalence and clinical feature(s) of SCA2.and SCA3/MJD in patients with Parkinsonism in a Mainland chinese population and to find the difference in the SCA2.and SCA3/MJD mutations between ataxic and parkinsonian *** and Methods CAG triplet repeat expansions of ATXN2.and MJD1 genes were analyzed in a cohort of 452.Mainland chinese patients affected by typical Parkinsonism,including 386 sporadic and 66 familial *** and direct sequencing of the expanded allele was performed in patients positive for the ATXN2.and MJD1 *** dopamine transporter (DAT) was evaluated in one SCA2.and one SCA3/MJD-positive family members using carbon(C)[C]-radiolabeled- CFT positron emission tomography(PET).Results We found two patients in one familial PD(FPD) family(1.5%) and two sporadic PD patients(0.5%) with expanded CAG repeats in the ATXN2.locus,The expansions ranged from 36 to 37 repeats and were interrupted by CAA as(CAG)n(CAA)(CAG)8 in all the ATXN2.expansion-positive ***,we found four patients in two FPD families(3%) and another three sporadic PD patients(0.8%) in the MJD1 *** expansions ranged from 58 to 73 repeats and were not interrupted by CAA or other *** patients had levodoparesponsive Parkinsonism without obvious cerebellar signs.[C]-CFT PET in affected members in SCA2.and SCA3/MJD families showed decrements of C-CFT uptake in all patients listed *** A mutation in SCA2.or SCA3/ MJD is one of the genetic causes of PD,specifically FPD,in China’s ***’s disease

关键词： spinocerebellar ataxia 2 Machado-Joseph disease/spinocerebellar ataxia type 3 Parkinson’s disease PET

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