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Prevention of pathological change and cognitive degeneration of Tg2576 mice by inoculating Aβ_(1-15) vaccine

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Science china(life sciences) 2008年第8期51卷 743-750页

作者： HU JinJia1, LI GuoYing2, WANG HuaQiao3, LIN Xian3 & YAO ZhiBin3 1 Department of Anatomy, Medical college of Shanghai Jiao Tong university, Shanghai 200025, china 2 Department of Anatomy, Preclinical college, Guangdong university of Pharmacy, guangzhou 51.006, china 3 Department of Anatomy, Zhongshan School of Medicine, sun yat-sen university, guangzhou 510080, china 1. Department of Anatomy Medical College of Shanghai Jiao Tong University Shanghai 200025 China 2. Department of Anatomy Preclinical College Guangdong University of Pharmacy Guangzhou 510006 China 3. Department of Anatomy Zhongshan School of Medicine Sun Yat-sen University Guangzhou 510080 China

This study aims to discuss the effect of preventing pathological changes and cognitive degeneration of Tg2576 mice by inoculating the subunit fragment of Aβ ***-two Tg2576 mice were randomly divided into four groups, each having eight mice:Group I, the control group, inoculated with adjuvants;Group II, the Aβ42 group, inoculated with Aβ42 vaccine;Group Ⅲ, the Aβ1―15 group, inoculated with Aβ1―15 vaccine;and Group IV, the Aβ36―42 group, inoculated with Aβ36―42 *** titer of the serum anti-body against Aβ42(Group II) was significantly higher than that of the control group(Group I), and a low level of antibodies could be detected in the brain homogenate in the three vaccine-inoculated *** water maze test showed that the Aβ42 group, Aβ1―15 group and Aβ36―42 group were obviously im-proved compared with the control *** cultured splenocytes sampled from each group were induced by Con A or their respective antigens, and the cell proliferation of the three vaccine-inoculated groups was significantly higher than that of the control *** the Aβ42 group, IL2 and IFN-γ were relatively low and IL4 and IL10 were relatively *** contrast, IL4 and IL10 were much higher in the Aβ1―15 group and IL2 and IFN-γ were much higher in the Aβ36―42 *** immunohistochemical test showed a large number of senile plaques in the brain cortex and hippocampus of the mice in the con-trol group, no senile plaque in the brain of the Aβ1―15 group and Aβ42 group mice, and a small number of senile plaques in the brain of the Aβ36―42 group *** results suggest that the subunit fragment of Aβ1―15 vaccine could prevent not only cognitive and behavioral degeneration but also Aβ deposition and formation of senile plaques in Tg2576 mice.

关键词： Alzheimer’s disease β-amyloid peptide vaccine Tg2576 mice senile plaque

The Study of Local Electrophysiology in Macular Diseases

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眼科学报 1998年第1期14卷 30-34页

作者： Guangwei Luo, Lezheng Wu, De-zheng WuZhongshan Ophthalmic Center, sun yat-sen university of Medical sciences , guangzhou 510060, china Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purposes: To observe the features and clinical utility values of local electroretinogram (LERG) and local visual evoked potentials(LVEP) in macular diseases. Methods : The spot stimuli were obtained by changing the shutters with different sizes of hole at the posterior of Ganzfeld ball. The recording electrode for LERG was made with the filament of platinum as blepharophthalmostat like. The LERG and LVEP were simultaneously recorded in macular ***: The exudative AMD, Stargardt's disease, central serous chorioretinopathy and idio-pathic macular hole had seriously decreased amplitudes of LERG and higher abnormal rates, comparing with atrophic AMD and idiopathic preretinal membranes in the region of 2.5° and 5° at macula. The abnormal rates in these diseases were higher for LERG than for LVEP. Conclusion: The LERG can objectively measure the variation of macular function in macu-lopathies. The sensitivities of LVEP are lower than those of LERG for macular diseases. Eye Science 1998; 14; 30 -

关键词：眼斑点病局部电生理学视觉感应电压

EXPERIMENTAL STUDY ON THE CORNEAL ENDOTHELIUM OF TRAUMATIC CATARACT

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眼科学报 1991年第3期7卷 156-160页

作者： Quanjin Xie Yunliang Wang Miaorong Shao Zhongshan Ophthalmic Center sun yat-sen university of Medical sciences guangzhou 510060, china Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China.

The cell morphology of corneal endothelium in 84 mice with experimental traumatic cataract was investigated with stained corneal buttons. In the experimental group, the boundaries between adjacent corneal endothelial cells were significantly distorted, some cell boundaries manifested degenerative changes that led to coalescence of the cells. The mean density and mean area of endothelial cells of the controls showed significant difference from those of the experimental group during the 12 weeks of observ...

关键词： EXPERIMENTAL STUDY ON THE CORNEAL ENDOTHELIUM OF TRAUMATIC CATARACT than

Inactivation gating determines drug potency:a common mechanism for drug blockade of HERG channels

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Acta Pharmacologica Sinica 2004年第5期25卷 12-18页

作者： Bao-feng YANG1, Dong-hui XU2, Chao-qian XU, Zhe LI, Zhi-min DU, Hui-zhen WANG, De-li DONGDepartment of Pharmacology, Harbin Medical university, Harbin 150086, china 2Department of Pharmacy, sun yat-sen university, guangzhou 510080, china Department of Pharmacology Harbin Medical University Department of Pharmacy Sun Yat-sen University

AIM: To determine the mechanisms of interactions between different drugs and HERG channels. METHODS:Various antiarrhythmic (dofetilide, quinidine, azimilide, RP58866) and non-antiarrhythmic (terfenadine, nicotine)agents were used on HERG channels expressed in Xenopus oocyte. Whole-cell voltage-clamp techniques wereused. RESULTS: All drugs produced concentration-dependent block of HERG current. The inhibition was mark-edly facilitated with voltage protocols favoring channel inactivation (eg, less negative holding potentials). Maneu-vers that weakened channel inactivation (eg, elevation of external K+), relieved HERG blockade by all drugs. Moreover,the inhibitory potency was reduced by at least 20-300 fold with varying compounds when rapid C-type inactivationwas removed by a mutation located between the transmembrane domains 5 and 6 (S631A). CONCLUSION: Theinactivation gating of HERG channels determines the blocking potency of drugs. This mechanism might be com-mon to drugs of various classes.

关键词： antiarrhythmic agents potassium channels mutation channel inactivation patch-clamp techniques oocytes Xenopus

A CONSANGUINEOUS MATING COUPLE AND THEIR CONCOMITANT ESOTROPIA TWINS

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眼科学报 1991年第3期7卷 153-155页

作者： Danyang Li Youzhao Chen Eye Institute, Zhongshan Ophthalmic Center sun yat-sen university of Medical sciences guangzhou 510060, china Eye Institute Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China.

A first cousin marriage couple and their 8-year-old identical twin daughters with concomitant strabismus are *** family would indicate an autosomal recessive inheritance in concomitant strabismus.

关键词： De A CONSANGUINEOUS MATING COUPLE AND THEIR CONCOMITANT ESOTROPIA TWINS PVEP RFLPs

Polycyclic Aromatic Hydrocarbons in Agricultural Soils of the Southern Subtropics,china

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Pedosphere 2007年第5期17卷 673-680页

作者： HAO Rong WAN Hong-Fu SONG Yan-Tun JIANG Hong PENG Shao-Lin South china Botanical Garden Chinese Academy of Sciences Guangzhou 510650 （China） Guangdong Key laboratory of Agricultural Environment Pollution Integrated Control Guangdong Institute of Eco- Environment and Soil Sciences Guangzhou 510650 （China） School of life Science Sun Yat-Sen University Guangzhou 510275 （China） Conservation biology Institute 260 SW Madison Ave. Suite 106 Corvallis OR 97333 （USA）

The contributions of persistent organic pollutants （POPs） from the subtropical regions of china to pollution of the global environment have been paid great attention; however, little is known about the state of POPs in agricultural ecosystems within these regions of china. This study primarily revealed the state of the contamination and distribution of polycyclic aromatic hydrocarbons （PAH） in agricultural soils in the subtropical regions. 115 surface soils （0-20 cm） were sampled in the breadbaskets of these regions. The concentrations and types of PAH were determined using gas chromatography linked to mass spectrometry （GC-MS）. The total PAH concentrations ranged from 22.1 to 1 256.9 ng g^-1 with a mean of 318.2 ± 148.2 ng g^-1. In general terms, the current PAH concentrations were lower than most PAH levels reported in a number of investigations from different countries and regions. PAH isomer ratios indicated that pyrolytic origins, such as fossil fuel combustion related to vehicle tail gas and industrial emissions, were the dominant sources of PAH in the southern subtropical areas of china. Although PAH concentrations decreased with decreasing pollution, population, and traffic density, to a great extent PAH compositions were similar throughout subtropical soils, with naphthalene, phenanthrene, fluoranthene, and benzo（b）fluoranthene being dominant.

关键词： agricultural soils polycyclic aromatic hydrocarbons subtropical region

COMPARISON OF THE EFFECTIVENESS BETWEEN ANTERIOR CAPSULOTOMY WITH Nd:YAG LASER AND WITH BENT NEEDLE IN PATIENTS WITH CONGENITAL CATARACT

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眼科学报 1991年第3期7卷 125-127,124页

作者： Zhende Lin Shaozhen Li Eye Hospital, Zhongshan Ophthalmic Center sun yat-sen university of Medical sciences guangzhou 510060,china Eye Hospital Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China.

The effectiveness of capsulotomy with Nd:YAG laser and with bent needle were compared in 50 patients(66 eyes) with congenital cataract. There were 24 patients(32 eyes) in the laser group and 26 patients(34 eyes) in the needle group. There were not significant differences in sex, age, pre-operative visual acuity and intraocular pressures, and classification of cataract between the two groups. The results show that patients in the laser group have better post-operative visual acuity. The reason is YAG las...

关键词： YAG COMPARISON OF THE EFFECTIVENESS BETWEEN ANTERIOR CAPSULOTOMY WITH Nd Nd

Elevated Vascular Endothelial Growth Factor Levels in the Vitreous of Patients With Proliferative Diabetic Retinopathy

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眼科学报 1999年第1期15卷 17-21页

作者： Juan Deng, De-Zheng Wu, Rulong GaoZhongshan Ophthalmic Center, sun yat-sen university of Medical sciences, guangzhou 510060 ,china Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Objective: To detect the levels of vascular endothelial growth factor (VEGF) in the vitreous of patients with proliferative diabetic retinopathy (PDR) and to investigate the possible role of VEGF in the development of neovascularization in PDR. Methods ; Undiluted vitreous samples and fasting venous blood samples were obtained from 27 patients with PDR and 14 subjects with idiopathic macular hole who underwent pars plana vitrectomy. The concentration of VEGF was determined by quantitative enzyme - linked immunosorbent assay (ELISA).Results: The level of vitreous VEGF in patients with PDR (median 0. 41ng/ml, range 0. 09- 11. 56ng/ml) was significantly elevated when compared with that in control subjects (median 0.017ng/ml, range 0.008-0.04ng/ml)(P<0. 001). The median of PDR patients' serum VEGF concentration was 0.19ng/ml (0. 090. 46ng/ ml) which was far lower than vitreous VEGF concentration (P<0. 05). Vitreous VEGF concentration was higher in PDR patients with retinal detachment than that in patient

关键词：血管内皮生长因子玻璃体眼糖尿病性视网膜病 ELISA

Macrophages in Human Epiretinal and Vitreal Membranes in Patients with Proliferative Intraocular Disorders

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Eye Science 1996年第1期16卷 28-32页

作者： Shibo Tang, Rulong Gao, De-zheng WuZhongshan Ophthalmic Center, sun yat-sen university of Medical sciences, guangzhou 510060, china Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China.

Purpose: Macrophages are versatile cells and have been known as a cellular component of epiretinal membranes of proliferative intraocular disorders (PID). However, the origin and functions of these cells in the membranes are not yet clear. In the present study we investigated the characterization of macrophages/monocytes in various types of human epiretinal membranes from patients with proliferative vitreortinopathy (PVR) , proliferative diabetic retinopathy (PDR) and ocular perforating injury by means of immunohistochemical techniques. Methods: A total of 49 epiretinal membranes of PID in which 24 were PDR specimens, 17 were PVR and 8 were proliferations after perforating eye injury were studied. Monoclonal antibodies against human macrophages, monocytes, HLA-DR antigen and interleukin-1 (IL-1) were used. The alkaline phosphatase anti-alkaline phosphatase (APAAP) technique was ***: The results showed that 19 out of 24 PDR specimens (79%), 15 out of 17 PVR specimens (88%) and 7 out of 8

关键词： vitreoretinal diseases macrophages cytokine