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Effects of intrathecal 6-hydroxydopamine,α1 and α2.adrenergic receptor antagonists on antinociception of propofol in mice

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Acta Pharmacologica Sinica 2005年第2期26卷 186-191页

作者： Zhi-jun GE~(1,2.,Yin-ming ZENG~1,Yong-fei TAN~2.1jiangsu province Key Laboratory of {2.,xuzhou medical college,xuzhou 2.1002.china ~2.0} province People's Hospital of Yixing,Yixing 2.42.0,china jiangsu province Key Laboratory of {2. Xuzhou Medical College Xuzhou China jiangsu province People's Hospital of Yixing Yixing China

Aim:To investigate the relationship between spinal cord norepinephrine,α1andα2.drenergic receptors and antinociception of propofol in ***:Kunming mice were *** tests were investigated with the tail-immersion test and the acetic acid-induced writhing *** effects of subcuta-neous（sc）,intrathecal（ith）and intracerebroventricular（icv）injection propofolon pain threshold were *** influences of pretreatment with ith 6-hydroxydopamine,α1R antagonist prazosin,or α2. antagonist yohimbine on theantinociception of propofol were ***:Significant antinociceptionwas produced by propofol（2.,50 mg/kg,sc）and propofol（2.,40μg,ith）in tail-immersion test and acetic the acid-induced writhing test（P2.,and 40 μg）did not produce any effect on pain threshold in mice（P>0.05）.The 6-hydroxydopamine（5 and 10μg）,prazosin（5 and 10μg）,oryohimbine（5 and 10μg）ith alone did not affect basal tai-flick latency（TFL）inconscious mice,but significantly reduced the TFL as measured by tail-immersiontest in propofol（50mg/kg,sc）-treated mice,compared with basal TFL and ve-hicle groups（P2..

关键词： propofol spinal cord antinociception prazosin yohimbine norepinephrine

Isoflurane preconditioning protects against ischemia-reperfusion injury partly by attenuating cytochrome c release from subsarcolemmal mitochondria in isolated rat hearts

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Acta Pharmacologica Sinica 2005年第7期26卷 813-820页

作者： Li-ping QIAN Shan-shan ZHU~2.Jun-li CAO Yin-ming ZENG jiangsu Provincial Key Lab of anesthesiology,jiangsu Institute of anesthesiology,xuzhou medical college,xuzhou 2.1002.china jiangsu Provincial Key Lab of anesthesiology Jiangsu Institute of Anesthesiology Xuzhou Medical College Xuzhou China

Aim:To examine if isoflurane preconditioning can attenuate ischemia/reperfusioninjury by reducing cytochrome c release from inner mitochondrial ***:Isolated hearts of Sprague-Dawley rats were perfused on *** were randomly assigned to a non-treated group(CON group,n=12.or three isoflurane preconditioning groups(0.5% ISC group,1.0% ISC group,and 2.0% ISC group;n=12..In the latter three groups,isoflurane was given atconcentrations of 0.5%,1.0%,and 2.0% for 15 min with 15-rain washout before30-min *** mitochondria of the myocardium were isolatedafter 60-min *** of the each heart was recorded,infarctsize of the hearts and contents of cytosolic cytochrome or mitochondrial cyto-chrome c were measured at the end of *** of isolated mito-chondria in the four groups was evaluated,***:Compared withthe CON group,cytosolic cytochrome c in 0.5% ISC group,1.0% ISC group,and2.0% ISC group were significantly decreased along with a significant increase ofmitochondrial cytochrome *** size of the hearts in the four groups were56%±12.,41%±12.,32.±7% and 33 %±11%,*** values of thethree isoflurane preconditioning groups were significantly lower than that of theCON group(P2.0% isofluraneon reducing cytochrome c release were more remarkable than 0.5% and 1.0%concentrations of ***:Myocardioprotective effects ofisofluranc preconditioning were associated with attenuation of cytochrome c lossfrom the inner membrane of subsarcolemmal mitochondria.

关键词： isoflurane reperfusion injury cytochromec mitochondria

Intrathecal administration of Cav3.2.and Cav3.3 antisense oligonucleotide reverses tactile allodynia and thermal hyperalgesia in rats following chronic compression of dorsal root of ganglion

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Acta Pharmacologica Sinica 2006年第12期27卷 1547-1552页

作者： Xian-jie WEN~(1,2.Zhang-jun LI~2.Zhi-xin CHEN~2.Zhi-yuan FANG~3 Chen-xiang YANG~1 Heng LI~1 Yin-ming ZENG~(2.4)1 Department of anesthesiology,the First People's Hospital of FoShan & Affiliated FoShan Hospital of Sun-Yat Sen University,Foshan 52.000,china {2. province Institute of anesthesiology,xuzhou medical college,xuzhou 2.1002.china 3 Department of anesthesiology,the Municipal Hospital of Suzhou,Suzhou 2.5002.china Department of anesthesiology the First People's Hospital of FoShan & Affiliated FoShan Hospital of Sun-Yat Sen University Foshan China jiangsu province Institute of {2. Xuzhou Medical College Xuzhou China Department of anesthesiology the Municipal Hospital of Suzhou Suzhou China

Aim:The present study aimed to elucidate the role of T-subtype calcium channels（Cav3.1,Cav3.2.and Cav3.3）in the pathogenesis of neuropathic pain at spinal ***:The chronic compression of the dorsal root ganglion（CCD）rat modelwas *** antisense oligonucleotide of Cav3.1,Cav3.2.and Cav3.3 or nor-mal saline（NS）were intrathecally administered twice per day from the first day tothe fourth day after *** mechanical withdrawal threshold and pawthermal withdrawal latency were measured to evaluate the tactile allodynia andthermal hyperalgesia,***:CCD rats developed reliable tactileallodynia and thermal hyperalgesia after *** administration ofantisense oligonucleotide of Cav3.2.and Cav3.3 significantly relieved tactileallodynia and thermal hyperalgesia in CCD rats,but not ***:Cav3.2.nd Cav3.3 subtype calcium channels in the spinal cord may play an important rolein the pathogenesis of neuropathic pain,which may contribute to the manage-ment of the neuropathic pain.

关键词： T-type calcium channels neuropathic pain spinal cord antisense oligonucleotide

Activation of ERK/CREB pathway in spinal cord contributes to chronic constrictive injury-induced neuropathic pain in rats

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Acta Pharmacologica Sinica 2005年第7期26卷 789-798页

作者： Xue-song SONG~2.Jun-li CAO~(3,4,5) Yan-bing XU~3 Jian-hua HE~3 Li-cai ZHANG~3 Yin-ming ZENG~(3,4)2.Department of anesthesiology,First Clinical college of N.Bethune Centre of Health Sciences,Jilin University,Changchun 13002.,{2. 3 Department of anesthesiology,Affiliated Hospital of xuzhou medical college,xuzhou 2.1002.china 4 jiangsu Institute of anesthesiology,xuzhou 2.1002.china Department of anesthesiology First Clinical College of N. Bethune Centre of Health Sciences Jilin University Changchun China Department of anesthesiology Affiliated Hospital of Xuzhou Medical College Xuzhou China jiangsu Institute of anesthesiology Xuzhou China

Aim:To investigate whether activation and translocation of extracellular signal-regulated kinase(ERK)is involved in the induction and maintenance of neuro-pathic pain,and effects of activation and translocation of ERK on expression ofpCREB and Fos in the chronic neuropathic ***:Lumbar intrathecalcatheters were chronically implanted in male Sprague-Dawley *** left sciaticnerve was loosely ligated proximal to the sciatica's trifurcation at approximately 1.0mm intervals with 4-0 silk *** mitogen-activated protein kinase kinase(MEK)inhibitor U012. or phosphorothioate-modified antisense oligonucleotides(ODN)were intrathecally administered every 12.h,1 d pre-chronic constrictioninjury(CCI)and 3 d *** and mechanical nociceptive thresholdswere assessed with the paw withdrawal latency(PWL)to radiant heat and vonFrey *** expression of pERK,pCREB,and Fos were assessed by bothWestern blotting and immunohistochemical ***:Intrathecal injec-tion of U012. or ERK antisense ODN significantly attenuated CCI-induced me-chanical allodynia and thermal *** significantly increased the ex-pression of p-ERK-IR neurons in the ipsilateral spinal dorsal horn to injury,not inthe contralateral spinal dorsal *** time courses of pERK expression showedthat the levels of both cytosol and nuclear pERK,but not total ERK,were in-creased at all points after CCI and reached a peak level on postoperative d *** significantly increased the expression of pCREB and ***-CREB-positive neurons were distributed in all laminae of the bilateral spinal cord and Foswas expressed in laminae I and II of the ipsilateral spinal dorsal *** of U012. or ERK antisense ODN markedly suppressed the increase ofCCI-induced pERK,pCREB and c-Fos expression in the spinal ***:The activation of ERK pathways contributes to neuropathic pain in CCI rats,andthe function of pERK may partly be accomplished via the cAMP

关键词： extracellular signal-regulated kinases cyclic AMP-response DNA-binding protein pain hyperalgesia spinal cord

Ginkgo biloba extract suppresses hypertrophy and extracellular matrix accumulation in rat mesangial cells

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Acta Pharmacologica Sinica 2006年第9期27卷 1222-1230页

作者： Jian-yun WANG~2.Xiao-xing YIN~(2.6)Yun-ming WU~2.Dao-quan TANG~2.Yuan-yuan GAO~2.Mei-rong WAN~4 Xiao-yu HOU~3 Bei ZHANG~5 2.Department of Pharmacy,3 Provincial Key Lab for Brain Diseases Bioinformation,xuzhou medical college,xuzhou 2.1002.china 4 Center-Laboratory,Affiliated Hospital of xuzhou medical college,xuzhou 2.1002.china 5 Department of Obstetrics and Gynecology,The Third People's Hospital of xuzhou,xuzhou 2.1002.china Department of Pharmacy Xuzhou Medical College Xuzhou China Center-Laboratory Affiliated Hospital of Xuzhou Medical College Xuzhou China Provincial Key Lab for Brain Diseases Bioinformation Xuzhou Medical College Xuzhou China Department of Obstetrics and Gynecology The Third People's Hospital of Xuzhou Xuzhou China

Aim:To observe the effects of Ginkgo biloba extract（EGb）on the hypertrophy ofmesangial cells and the accumulation of extracellular matrix（ECM）in ***:Cultured mesangial cells were allotted into 7 groups:normal group,solvent control group,high glucose group,low dose of EGb group,moderatedose of EGb group,high dose of EGb group,and captopril *** of cellantioxidases,S phase percentage and G0/G1phase percentage,collagen Ⅳ andlaminin,Smad2.3 and Smad7,TGF-β1mRNA were measured by different ***:For EGb-treated groups,when compared with high glucose group,thecell percentage of S phase was raised and the percentage of G0/G1was *** intensity of oxidative stress was *** expression of Smad2.3 wasgreatly decreased and Smad7 was *** Ⅳ,laminin and TGF-β1mRNA were also ***:EGb can suppress cell hypertrophy andthe accumulation of ECM in rat mesangial cells,which means it could play a vitalrole in the delay of glomerulosclerosis in diabetic nephropathy.

关键词： ginkgo biloba extract glomerulosclerosis oxidative stress TGF-β1 Smads

GABAA receptor partially mediated propofol-induced hyperalgesia at superspinal level and analgesia at spinal cord level in rats

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Acta Pharmacologica Sinica 2004年第12期25卷 53-59页

作者： Qin-yun WANG2.3, Jun-li CAO2. Yin-ming ZENG2. Ti-jun DAI2.4 2.0} Institute of anesthesiology, jiangsu province Key Laboratory of anesthesiology, xuzhou, 2.1002, china 3Department of anesthesiology, the1st Affiliated Hospitalof Soochow University, Suzhou 2.5006, china 江苏省麻醉学研究所江苏省麻醉学重点实验室 Xuzhou 221002

AIM: To observe effects of propofol on nociceptive response at superspinal and spinal level in rats. METHODS: Two hundreds and fifty-eight Sprague-Dawley male rats were randomized into thirty-two groups. Propofol and bicuculline were microinjected into lateral ventricle (icv), ventrolateral periaqueductal gray (vlPAG), intrathecal (ith), and intraperitoneal (ip). The noxious responses were evaluated by hot plate and formalin test. RESULTS: In hot-plate test, systemic and superspinal administration of propofol (40 mg·kg-1 ip, 100 μg in 10 μL, icv, and 4 μg in 0.4 μL vlPAG microinjection) produced hyperalgesia (P2.%, 98.8 % at 10, 2., and 30 min by microinjection of bicuculline (10 ng in 0.4 μL, vlPAG) (P2., 30 and 40 min by ith bicuculline (P<0.05). In formalin test, systemic and superspinal administration of propofol (40 mg·kg-1 ip, 4 μg in 0.4 μL, vlPAG) also produced hyperal- gesia (P<0.01). The increased formalin pain scores were antagonized about 57.1 % by bicuculline (10 ng, vlPAG) (P<0.05) at 60 min after formalin injection. The decreased formalin pain scores induced by ith propofol (100 μg in 10 μL) were antagonized about 66.7 % at 30 min by ith bicuculline (P<0.05) after formalin injection. Hyperalgesia produced by ip propofol in both hot plate and formalin test could not be antagonized by vlPAG administration of bicuculline. CONCLUSION: GABAA receptor partly mediated propofol-induced hyperalgesia at superspinal and analgesia at spinal cord in rats.

关键词： GABA receptors propofol pain ventrolateral periaqueductal gray spinal cord

Effects of Ginkgo biloba on prevention of development of experimental diabetic nephropathy in rats

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Acta Pharmacologica Sinica 2007年第6期28卷 818-828页

作者： Qian LU~2.Xiao-xing YIN~(2.3),Jian-yun WANG~2.Yuan-yuan GAO~2.Ying-mei PAN~2.2.Department of Pharmacy,xuzhou medical college,xuzhou 2.1002.china Department of Pharmacy Xuzhou Medical College Xuzhou China

Aim:To observe the preventive and therapeutic effects of Ginkgo biloba extract(GbE) on early experimental diabetic nephropathy (DN) in ***:After anearly DN model was induced by streptozotocin,rats were administered GbE at 3doses for 12.*** blood glucose,creatinine (Cr),blood urea nitrogen(BUN),urine protein,kidney index,anti-oxidase,advanced glycosylation end prod-ucts (AGE),collagen IV and laminin,matrix metalloproteinases-2.(MMP-2. and thetissue inhibitor of metalloproteinase-2.(TIMP-2.,connective tissue growth factor(CTGF),and transforming growth factor-β1 (TGF-β1) mRNA were measured bydifferent *** ultrastructural morphology and the thickness of glomeru-lar base membrane (GBM) were observed by a transmission electron ***:For the GbE-treated DN rats,when compared with the vehicle-treatedDN rats,the fasting blood glucose level,Cr,BUN,urine protein level,and theintensity of oxidative stress were significantly *** expression of MMP-2.greatly increased,and TIMP-2.***,AGE,either in serum or in renal,the collagen IV,laminin,CTGF levels,and TGF-β1 mRNA were ***,both relative grades of mesangium hyperplasia by microscopicalobservation and the thickness of GBM by electron microscope measurement de-creased ***:GbE has protective effects on several pharma-cological targets in the progress of DN and is a potential drug for the preventionof early DN.

关键词： Ginkgo biloba extract diabetic nephropathy transforming growth factor-131 matrix metalloproteinases-2 connective tissue growth factor

Inhibition of Proliferation and Induction of Apoptosis in Human Renal Carcinoma Cells by Anti-telomerase Small Interfering RNAs

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 500-506页

作者： Jun-Nian ZHENG~(1,3#*)Ya-Feng SUN~(2.)Dong-Sheng PEI~2.Jun-Jie LIU~1 Jia-Cun CHEN~1 Wang LI~1 Xiao-Qing SUN~1 Qi-Duo SHI~3 Rui-Fa HAN~3 Teng-Xiang MA~3 1 Laboratory of Urology,Affiliated Hospital of xuzhou medical college,xuzhou 2.1002.china 2.Research Center for Biochemistry and Molecular Biology,xuzhou medical college,xuzhou 2.1002.china 3 Institute of Urology,Tianjin medical University,Tianjin 300070,china Laboratory of Urology Affiliated Hospital of Xuzhou Medical CollegeXuzhou 221002 China Institute of Urology Tianjin Medical UniversityTianjin 300070 China Research Center for Biochemistry and Molecular Biology Xuzhou Medical CollegeXuzhou 221002 China

Telomerase is an attractive molecular target for cancer therapy because it is present in mostmalignant cells but is undetectable in most normal somatic *** telomerase consists of two subunits,an RNA component(hTR)and a human telomerase reverse transcriptase component(hTERT).Smallinterfering RNA(siRNA),one kind of RNA interferences,has been demonstrated to be an effective methodto inhibit target gene expression in human *** investigated the effects of siRNA targeting at both hTRand hTERT mRNA on the inhibition of telomerase activity in human renal carcinoma cells(HRCCs).Theproliferation and apoptosis of HRCCs were *** treatment of HRCCs using hTR and hTERTsiRNAs resulted in significant decrease of hTR mRNA,hTERT mRNA and hTERT *** siRNA canalso inhibit the telomerase activity and the proliferation of ***,they can induce apoptotic celldeath in a dose-dependent *** these findings,we propose that the inhibition of telomerase activityusing siRNA targeting hTR and hTERT might be a rational approach in renal cancer therapy.

关键词： hTERT hTR telomerase siRNA renal cell carcinoma proliferation apoptosis

Calculation of maximum ground movement and deformation caused by mining

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中国有色金属学会会刊：英文版 2011年第S3期21卷 562-569页

作者： LI Pei-xian1, 2. TAN Zhi-xiang1, 2. DENG Ka-zhong1, 2.1. Key Laboratory for Land Environment and Disaster Monitoring of State Bureau of Surveying and Mapping, china University of Mining and Technology, xuzhou 2.1116, china 2. jiangsu Key Laboratory of Resources and Environmental Information Engineering, china University of Mining and Technology, xuzhou 2.1116, china Key Laboratory for Land Environment and Disaster Monitoring of State Bureau of Surveying and Mapping China University of Mining and Technology Xuzhou 221116 China jiangsu Key Laboratory of Resources and Environmental Information Engineering China University of Mining and Technology Xuzhou 221116 China

In order to know the maximum mining ground deformation of arbitrary surface point, directions of the maximum surface tilt, curvature,horizontal displacement and horizontal deformation caused by multi-working faces were deduced based on probability integral method. The distribution forms of surface deformations in all direction φ∈[0, 2.] were obtained and also equations of maximum deformations were given based on calculation of mining ground deformation in direction φ. A mining subsidence analysis system was developed with VB6.0. The system implements the probability integral mining subsidence prediction with direct integration method, and it can avoid errors of arbitrary shaped working face subdivision of traditional method, and accuracy of mining subsidence prediction can be improved with the direct integral method. The system implements the contour chart and profile chart and also data analysis automation by manipulation of the SURFER kernel function and it complements the defect of existing subsidence prediction software. Calculation of maximum deformations caused by multi-working faces mining in a coal mine of xuzhou, china was shown as application example. Engineering application indicates that maximum deformation of mining ground surface can be calculated and analyzed by the system. The research provides a theoretical basis and calculation tool for mining subsidence prediction and analysis.

关键词： mining subsidence probability integral method movement and deformation system development

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