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science china(Life sciences) 2005年第2期48卷 187-194页

作者： WANG Genyu1,YAN Bingxue1,DENG Xuemei2,LI Changlü2,HU Xiaoxiang1 & Li Ning1 1.State Key Laboratory for Agrobiotechnology,china agricultural university,beijing 100094,china 2.department of animal genetics and breeding,college of animal science and technology,china agricultural university,beijing 100094,china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China department of animal genetics and breeding College of Animal Science and Technology China Agricultural University Beijing 100094 China

We have identified DNA polymorphisms in the gene of insulin-like growth factor 2 by PCR-SSCP in a resource population, which was generated by Silky reciprocally crossing to Broilers. A C→G mutation was detected in the exon 2 (at position 71) by sequencing. This single nucleotide polymorphism (SNP) was found to be associated with production traits. Chicken with BB genotype showed more chest angle width but less 3 week body weight and glandular stom- ach weight than chicken with AA genotype (P2 locus was imprinting. Progeny from heterozygote × homozygote reciprocal cross was assayed for ex- pression after the genotype was determined. The transcription of IGF2 was detected by RT-PCR-SSCP. IGF2 gene was expressed bialleleically in 1-day-old neonatal liver and 90-day-old liver, kidney, heart, and muscle of both heterozygote AB and BA chickens. Therefore, IGF2 was not an imprinting gene in chicken. The different genetic effects between the het- erozygote AB and BA remain to be elucidated.

关键词： chicken, IGF2, single nucleotide polymorphism, genome imprinting.

Simulation study on the efficiencies of MOET nucleus breeding schemes applying marker assisted selection in dairy cattle

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science china(Life sciences) 2009年第3期52卷 296-306页

作者： LUO WeiZhen,WANG YaChun & ZHANG Yuan department of animal genetics & breeding,college of animal science and technology,china agricultural university,beijing 100094,china department of animal genetics & breeding College of Animal Science and Technology China Agricultural University Beijing China

Advantages of breeding schemes using genetic marker information and/or multiple ovulation and embryo transfer(MOET) technology over the traditional approach were extensively evaluated through *** yield was the trait of interest and QTL was the genetic marker *** dairy cattle breeding scenarios were considered,i.e.,traditional progeny testing breeding scheme(denoted as STANPT),GASPT scheme including a pre-selection of young bulls entering progeny testing based on their own QTL information,MOETPT scheme using MOET technology to generate young bulls and a selection of young bulls limited within the full-sib family,GAMOPT scheme adopting both QTL pre-selection and MOET technology,COMBPT scheme using a mixed linear model which considered QTL genotype instead of the BLUP model in GAMOPT,and three non-progeny testing schemes,*** MOET,GAMO and COMB schemes,corresponding to MOETPT,GAMOPT and COMBPT with progeny testing being part of the *** were selected based on their breeding value which was estimated under an animal model *** selection over 17 years was performed in the simulations and 30 replicates were designed for each *** influences of using QTL information and MOET technology on favorable QTL allele frequency,true breeding values,polygenetic breeding values and the accumulated genetic superiority were extensively evaluated,for five different populations including active sires,lactating cows,bull dams,bull sires,and young *** results showed that the combined schemes significantly outperformed other approaches wherein accumulated true breeding value *** difference between schemes exclusively using QTL information or MOET technology was not *** STANPT scheme was the least efficient among the 8 *** schemes using MOET technology had a higher polygenetic response than others in the 17th *** increases of frequency of the favorable QTL allele varied more greatly across t

关键词： dairy cattle marker-assisted selection two-stage selection MOET QTL

Expression of the Tribolium castaneum (Coleoptera: Tenebrionidae) hsp83 gene and its relation to oogenesis during ovarian maturation

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Journal of genetics and Genomics 2010年第8期37卷 513-522页

作者： Jingjing Xu 1, Juan Shu 1, Qin Zhang Key Laboratory of animal genetics and breeding of the Ministry of Agriculture, college of animal science and technology, china agricultural university, beijing 100193, china Key Laboratory of animal genetics and breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing 100193 China

Heat shock proteins （HSP） can protect organisms and cells from thermal damage. In this study, we cloned the full length cDNA encoding the HSP83 protein （the homologue of HSP90） of Tribolium castaneum（red flour beetle）. The isolated cDNA contains the full coding sequence, a partial 5＇ untranslated region of 55 bp and the complete 3＇ untranslated region. We found the hsp83 gene is located on chromosome 5 of the T. castaneum genome. The predicted HSP83 protein sequence has a high similarity （on average 86.77%） with that of other insect species. The expression of the hsp83 gene in the whole body and in the ovary could be induced with heat stress （40℃ for 1 h） in newly hatched （within 3 h post emergence） and mature （10 days post emergence） beetles. Under normal conditions, the hsp83 expression in the ovary is about 3-fold higher than in the whole body at both stages. No significant difference in hsp83 expression was observed between the two ovarian developmental stages regardless if the beetles were treated with heat shock or not. The expression of the HSP83 protein in the whole body could also be induced with heat stress in newly hatched and mature beetles. However, in the ovary, HSP83 was only expressed in the follicle cells of mature beetles and not in newly hatched beetles, regardless if the beetles were treated with heat shock or not. Furthermore, the females were not able to produce mature oocytes after knock-down of the hsp83 expression by injecting dsRNA. These results suggest that the HSP83 protein is involved in protection against heat stress and could be involved in oogenesis during ovarian maturation of T. castaneum.

关键词： hsp83 cDNA cloning Tribolium castaneum expression heat shock

Fine mapping quantitative trait loci affecting milk production traits on bovine chromosome 6 in a Chinese Holstein population

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Journal of genetics and Genomics 2009年第11期36卷 653-660页

作者： Gui Mei 1,Cengceng Yin 1,Xiangdong Ding,Qin Zhang State Key Laboratory for Agrobiotechnology,Key Laboratory of animal genetics and breeding of the Ministry of Agriculture,college of animal science and technology,china agricultural university,beijing 100193,china college of animal science and technology China Agricultural University State Key Laboratory for Agrobiotechnology Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture Beijing 100193

To fine map the previously detected quantitative trait loci （QTLs） affecting milk production traits on bovine chromosome 6 （BTA6）, 15 microsatellite markers situated within an interval of 14.3 cM spanning from BMS690 to BM4528 were selected and 918 daughters of 8 sires were genotyped. Two mapping approaches, haplotype sharing based LD mapping and single marker regression mapping, were used to analyze the data. Both approaches revealed a quantitative trait locus （QTL） with significant effects on milk yield, fat yield and protein yield located in the segment flanked by markers BMS483 and MNB209, which spans a genetic distance of 0.6 cM and a physical distance of 1.5 Mb. In addition, the single marker regression mapping also revealed a QTL affecting fat percentage and protein percentage at marker DIK2291. Our fine mapping work will facilitate the cloning of candidate genes underlying the QTLs for milk production traits.

关键词： fine mapping QTL Bos Taurus autosome 6 milk production traits

Reduction of drosopterin content caused by a 45-nt insertion in Henna pre-mRNA of Drosophila melanogaster

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science china(Life sciences) 2008年第8期51卷 702-710页

作者： WANG Qin, ZHAO ChunJiang, BAI LiHua, DENG XueMei & WU ChangXin State Key Laboratory of Agrobiotechnology & Key laboratory of animal genetics and breeding of the Ministry of Agriculture, college of animal science and technology, china agricultural university, beijing 100193, china State Key Laboratory of Agrobiotechnology & Key laboratory of animal genetics and breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing China

Phenylalanine hydroxylase is assumed to be a key enzyme in drosopterin metabolism, but direct in vivo evidence to support this hypothesis is still *** the present study, we found a new natural reces-sive purple eye mutant of Drosophila melanogaster, Hnbp, which was a 45-nt insertion mutant in the second exon of *** insertion resulted in a predicted protein with 15 additional amino acids as compared to the wild-type *** analysis of protein structure showed that the predicted mutant protein probably had two more β-sheets, which may cause instability of two α-helices near the catalytic centre of the enzyme in the Biopterin-Hydroxyl binding *** mutant showed eye color defect with decrease of mRNA level, as well as drosopterin content *** drosopterin defect could be fully rescued by expression of wild type Henna in the Hnbp background by GMR-GAL4 UAS-Henna/UAS-Henna:Hnbp/Hnbp transgenic *** taken together, it can be concluded that the mu-tation in Henna is responsible for drosopterin reduction in mutant Hnbp, which provides key in vivo evidence to support the hypothesis that Henna is involved in drosopterin synthesis.

关键词： Drosophila melanogaster Henna gene drosopterin phenylalanine hydroxylase Hnbp

Genetic polymorphisms of milk epithelial mucin of yaks

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Progress in Natural science:Materials International 2006年第11期16卷 1222-1226页

作者： ZHENG Yucai~1, ZHAO Xingbo~2**, JIN Suyu~1, PENG Xianwen~1 and BAI Wenlin~1 (1. college of Life science and technology, Southwest university for Nationalities, Chengdu 610041, china 2. State Key Laboratory for Agrobiotechnology and Ministry of Agriculture Key Laboratory of animal genetics, breeding and Reproduction, china agricultural university, beijing 100094, china) college of Life science and technology Southwest University for Nationalities Chengdu 610041 China State Key Laboratory for Agrobiotechnology Ministry of Agriculture Key Laboratory of Animal Genetics Breeding and Reproduction China Agricultural University Beijing 100094 China

Milk epithelial mucin (MUC1) of yaks was separated by SDS-polyacrylamide gel electrophoresis. A total of nine types of MUC1 were revealed in 427 yaks from five yak breeds including Maiwa yak, Jiulong yak, Tianzhu white yak, Qinghai yak and Tibetan yak. The molecular weights of MUC1 are from 163 kD to 208 kD, most of which are larger than those of bovine. Population genetic analysis shows that the gene frequency and genotype frequency of yak MUC1 differ among breeds, with relatively high gene heterozygosity. The five yak breeds studied fall into two groups according to their milk MUC1 gene frequency, suggesting that milk MUC1 exhibits specificities for local yak groups.

关键词： yak milk genetic polymorphism MUC1.

Aberrant DNA methylation patterns in cultured mouse embryos

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Progress in Natural science:Materials International 2005年第12期15卷 1079-1083页

作者： HOU Jian,CUI Xiuhong,LEI Tinghua,LIU Lei,AN Xiaorong and CHEN Yongfu State Key Laboratory for Agrobiotechnology and department of animal Physiology,college of Biological science,china agricultural university,beijing 100094,china State Key Laboratory for Agrobiotechnology and department of animal Physiology College of Biological Science China Agricultural University Beijing 100094 China

Mouse early embryos undergo genome-wide demethylation and remethylation events during pre-implantation *** methylation reprogramming is thought to be associated with development *** immunofluorescence staining with an antibody against 5-methylcytosine (MeC),we examined the genome methylation patterns of mouse embryos cultured in *** results did not show the difference in staining patterns between development-blocked two-cell embryos that cultured in vitro and the two-cell embryos that were freshly collected from the donor *** in vitro-arrested morulae displayed a strong positive staining when compared to the morulae freshly collected from the donor *** the blastocyst stage,although most embryos showed the expected methylation patterns,with highly stained inner cell mass (ICM) and weekly stained trophectoderm (TE),a proportion of embryos were dimly stained in both ICM and *** results indicated that the methylation profile of the embryos could be changed by culturing in vitro when the embryos were in the transition from morulae to blastocyst.

关键词： methylation mouse embryo 5-methylcytosine antibody

Identification of yak lactate dehydrogenase B gene variants by gene cloning

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science china(Life sciences) 2008年第5期51卷 430-434页

作者： ZHENG YuCai1, ZHAO XingBo2, ZHOU Jing1, PIAO Ying1, JIN SuYu1, HE QingHua1, HONG Jian1, LI Ning2 & WU ChangXin2 1 college of Life science and technology, Southwest university for Nationalities, Chengdu 610041, china 2 State Key Laboratory for Agrobiotechnology and Ministry of Agriculture Key Laboratory of animal genetics and breeding, college of animal science and technology, china agricultural university, beijing 100094, china college of Life science and technology Southwest University for Nationalities Chengdu China State Key Laboratory for Agrobiotechnology and Ministry of Agriculture Key Laboratory of animal genetics and breeding College of Animal Science and Technology China Agricultural University Beijing China

Native polyacrylamide gel electrophoresis showed that two types of lactate dehydrogenase (LDH) existed in yaks. Based on the electrophoresis characteristics of LDH isoenzymes, yak LDH variants were speculated to be the gene mutation on H subunit encoded by B gene. According to the mobility in electrophoresis, the fast-band LDH type was named LDH-Hf and the slow-band LDH type LDH-Hs. In order to reveal the gene alteration in yak LDH variants, total RNA was extracted from heart tissues of yaks with different LDH variants, and cDNAs of the two variants were reverse transcripted. Two variants of B genes were cloned by RT-PCR. Sequence analysis revealed that four nucleotides differed between LDH-Bf and LDH-Bs, which resulted in two amino acids alteration. By Deepview software analysis of the conformation of yak LDH1 variants and H subunit, these four nucleotides altered two amino acids that generated new hydrogen bonds to change the hydrogen bonds network, and further caused subtle conformational changes between the two LDH variants.

关键词： yak lactate dehydrogenase genetic variants gene cloning molecular modeling

Origin and domestication history of Peking ducks deltermined through microsatellite and mitochondrial marker analysis

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science china(Life sciences) 2009年第11期52卷 1030-1035页

作者： QU LuJiang, LIU Wei, YANG FangXi, HOU ZhuoCheng, ZHENG JiangXia, XU GuiYun & YANG Ning State Laboratory for Agrobiotechnology and department of animal science and technology, china agricultural university, beijing 100193, china State Laboratory for Agrobiotechnology and department of animal science and technology China Agricultural University Beijing China

In order to elucidate the domestication history of Peking ducks, 190 blood samples from six Chinese indigenous duck breeds were collected with186 individualsgenotyped by 15 microsatellite markers. Both the FST and Nei’s standard genetic distances (Ds) from the microsatellite data indicated high genetic differentiation between Peking duck and other Chinese indigenous breeds. The haplotype network with mtDNA data showed that most of the Peking duck haplotypes were distinctly different from those of other domestic breeds. Although the H01 haplotype was shared by all domesticated duck breeds, Peking ducks displayed 12 specific domestic duck haplotypes, including four similar haplotypes H02, H04, H08 and H22, that formed a single haplogroup (A). Both H02 and H22 haplotypes were also shared by mallard and Peking ducks, indicating that Peking ducks originated from wild mallard ducks.

关键词： Peking duck Chinese indigenous duck breeds microsatellite mitochondrial DNA genetic relationship

Effect of leptin on oocyte maturation and subsequent pregnancy rate of cloned embryos reconstructed by somatic cell nuclear transfer in pigs

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Progress in Natural science:Materials International 2008年第12期18卷 1583-1587页

作者： Hengxi Wei~a Qiuyan Li~(a,b) Jun Li~a Yan Li~b Yunping Dai~(a,b) Yufang Ma~a Kai Xue~a Ning Li~a a State Key Laboratory for Agrobiotechnology and department of animal Physiology,china agricultural university,beijing 100094,china b Ji Pulin Biotech Ltd.,beijing 100094,china State Key Laboratory for Agrobiotechnology and department of animal Physiology China Agricultural University Beijing 100094 China Ji Pulin Biotech Ltd. Beijing 100094 China

Cloning pigs by somatic cell nuclear transfer (SCNT) has wide applications in basic research,human medicine and agricultural *** improve cloning efficiency,the effect of two basic maturation media,NCSU-23 and TCM199,was compared,and TCM199 was selected for the following experiments with *** systematically studied the effects of leptin supplementation on oocytes in vitro maturation (IVM),in vitro development of parthenogenetically activated (PA) and SCNT embryos and in vivo develop- ment of SCNT embryos after embryo transfer (ET).The results showed that supplementation of 100 or 200 ng/ml leptin into the mat- uration medium did not greatly affect nuclear maturation of oocytes,or cleavage rates of PA and SCNT (P>0.05).Blastocyst rates of PA and SCNT embryos were significantly improved when 100 or 200 ng/ml leptin was added to maturation medium,and the number of cells in PA blastocysts was also improved (P200 ng/ml leptin to the IVM medium may improve pregnancy rate and the delivery rate in pig cloning.

关键词： Leptin Oocytes IVM SCNT Pig