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Studies on hybridization effects of different geographic populations of Chlamys farreri Ⅱ.The medium-term growth and development of Chlamys farreri populations from China and Russia and their reciprocal crosses

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Acta Oceanologica Sinica 2003年第2期22卷 255-264页

作者： LiuXiaolin ChangYaqing XiangJianhai LiFuhua LiuXianjie BrovkinaElenaPavlovna collegeofAnimalscienceandTechnology NorthwestSci-TechUniversityofAgricultureandForestryYangling712100China KeyLabofMarineEcologyinAquacultureofMinistryofagriculture DalianFisheriesUniversityDalian116023China ExperimentalMarineBiologyLaboratory InstituteofOceanologyChineseAcademyofSciencesQingdao266071China KeyLabofMarineEcologyinAquacultureofMinistryofagricultureofMinistryofagriculture DalianFisheriesUniversityDalian116023China Far-EastStateTechnicalFisheriesuniversity Vladivostok690000Russia

Based on the research of juvenile (2. 3, 4 months) growth and survival of three populations of two different geographic areas in Chlamys farreri from Russian and China and their F1 hybrids derived from Chinese cultural population (CC) (?) × Russian population (RW) (?) , Chinese wild population (CW) (?) × Russian population (RW) (?), Russian population (RW) (?) × Chinese wild population (CW) (?) , the study of the medium-term (6, 8, 10, 12.months) growth and development of Chlamys farreri was carried out. The four determined results indicated that there existed different extent heterosis (3% -52.) for the growth in three types of F1 hybrids, and the offspring derived from CC(?) ×R(?) had a stronger heterosis among the crosses at the medium-term; the uptrend among traits are wet weight >shell width>shell length> shell height, Chinese cultural population could be recognized as excellent parent, and seasonal variations influence very much on the daily increment and growth rate of each trait of Chlamys farreri and it is only able to survive and could barely grow in winter (6-8 months), but grows fast in temperate season (10-12.months).

关键词： Chlamys farreri population hybridization medium-term growth and development heterosis

Strain Mesorhizobium ***035:A Stress-Tolerant Isolate from Glycyrrhiza glabra Displaying a Wide Host Range of Nodulation

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Pedosphere 2008年第1期18卷 102-112页

作者： WEI Ge-Hong YANG Xue-Ying ZHANG Zhi-Xin YANG Ya-Zhen K. LINDSROM Shaanxi Key Laboratory of Molecular Biology for agriculture College of Life Science Northwest A＆F University Yangling Shaanxi 712100 （China） Department of Applied Chemistry and Microbiology Biocenter 1 Fin-0014 University of Helsinki （Finland）

Thirty-nine rhizobial isolates were isolated from the root nodules of Glycyrrhiza uralensis and Glyeyrrhiza glabra, growing in the arid and semiarid regions of northwestern China, to test their taxonomic position and stress tolerance and to select one promising putative inoeulant strain for further studies. On the basis of 113 physiological and biochemical characteristics, the isolates were clustered into three groups. One isolate CCNWGX035 was found to have high tolerance to NaC1, pH, and temperature. By sequencing the 16S rDNA, isolate CCNWGX035 was placed in genus Mesorhizobium. Nodulation tests demonstrated that the isolate not only formed nitrogen-fixing nodules on its original host plant Glyeyrrhiza glabra, but also on Sophora vieiifolia, Lotus cornieulatus, Trifolium repens, Melilotus suaveolens, and Sophora alopecuroides. On the basis of sequence analysis of the nodA gene, isolate CCNWGX035 was closely related to strains of the genus Mesorhizobium, exhibiting some novel characteristics of root nodule bacteria.

关键词： Glycyrrhiza host range Mesorhizobium nodA stress tolerance

Possible Effect of 30K Proteins in Embryonic Development of Silkworm Bombyx mori

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Acta Biochimica et Biophysica Sinica 2005年第5期37卷 355-361页

作者： Bo-Xiong ZHONG~1 Jian-Ke LI~(1.2. Jian-Rong LIN~3 Jian-She LIANG~4 Song-Kun SU~1 Hai-Sheng XU~1 Hai-Yan YAN~1 Ping-Bo ZHANG~5 Hiroshi FUJII~5 1 Laboratory of Molecular Biology of Silkworm and Honeybee, Department of Bioresource science, college of Animal science,Zhejiang university, Hangzhau 31002., China 2.Institute of Apicultural Research, Chinese Academy of Agricultural science, Beijing 100093, China 3 Department of Sericulture, South China Agricultural university.Guangzhou 510642. China 4 Biochemistry Co. Ltd. of Zhejiang university, Hangzhou 31002.. China 5 Faculty of agriculture,Kyushu university, Fukuoka 812.81. Japan Laboratory of Molecular Biology of Silkworm and Honeybee Department of Bioresource Science College of Animal Science Zhejiang UniversityHangzhou 310029 China Institute of Agicultural Research Chinese Academy of Agricultural ScienceBeijing 100093 China Department of Sericulture South China Agricultural UniversityGuangzhou 510642 China Biochemistry Co. Ltd. of Zhejiang universityHangzhou 31002. China Faculty of agriculture Kyushu UniversityFukuoka 812-81 Japan

The silkworm Bombyx mori possesses a 30K protein family of 3×10～4 Da,the biologicalfunctions of which have not been fully *** relationship between the 30K protein family and theembryonic development of temperature sensitive sex-linked mutant strain of silkworm was investigated bytwo dimensional polyacrylamide gel electrophoresis(2.-PAGE)and Matrix assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS).The results show that protein spots 1-5 of the 30Kprotein family,mainly existing in normal strain,are possibly related to embryonic *** earlyconsumption of a 30K protein named 6G1-30K-1 and the accumulation of 30K proteins named 6G1-30K-3and 6G1-30K-4 are likely caused by the destruction of physiological balance in normal embryonic development,which may lead to lower hatchability of the temperature sensitive *** results suggest that reasonablemetabolism of 30K proteins is a prerequisite for the embryo's normal development.

关键词： silkworm Bombyx mori 30K protein embryonic development 2.-PAGE MALDI-TOF MS

Bioinformatic analysis of expressed sequence tags from sporophyte of Porphyra yezoensis (Bagiaceae, Rhodophyta)

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Progress in Natural science:Materials International 2006年第1期16卷 39-49页

作者： XU Minjun1, MAO Yunxiang1,ZHANG Xuecheng1, ZHOU Xiaojun1, SUI Zhenghong1, ZHOU Hailin2.and LI Jinhong 2.1.Laboratory of Marine Genetics and Breeding, college of Marine life {2.s, Ocean university of China, Qingdao 2.6003, China 2.James D Watson Institute of Genome {2.s, Zhejtang university / Key Laboratory of Genomic Bioinformatics of Zhejiang Province, Hangzhou Genomics Institute, Hangzhou 310008, China) Laboratory of Marine Genetics and Breeding College of Marine Life Sciences Ocean University of China Qingdao 266003 China James D Watson Institute of Genome sciences Zhejiang University/Key Laboratory of Genomic Bioinformatics of Zhejiang Province Hangzhou Genomics Institute Hangzhou 310008 China

A total of 719 expressed sequence tags （EST） clustered into 32. non-redundant EST groups are obtained from the sporophyte cDNA library of red algae, Porphyra yezoensis. Gene Ontology （GO） analysis is employed in characterizing 60 strictest annotated unique genes out of the 32. EST groups and some domains such as COX1, SodFeC, GST-N, SHMT, and RNase-PH related to the enzymes and proteins functioning in cells have been identified by HMMPFAM search. As its leafy gametophyte, the similar codon usage with strong bias is found in P. yezoensis filamentous sporophyte, regardless of some differences found in given amino acids. The average GC content of the 32. unique genes is 53. 0 % . In contrast, the third nucleotide of codon exhibits a higher GC content （ 72.% ） than that of the first （58%） and the second （42.） nucleotides. Similarity search of the present study shows a novel EST ratio of 60.2., which is against the Porphyra ESTs database, suggesting further investigations towards elucidating the characteristics of Porphyra functional genome.

关键词： Porphyra yezoensis expressed sequence tags (EST) bionformatic analysis.

Expression of the Tribolium castaneum (Coleoptera: Tenebrionidae) hsp83 gene and its relation to oogenesis during ovarian maturation

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Journal of Genetics and Genomics 2010年第8期37卷 513-522页

作者： Jingjing Xu 1, Juan Shu 1, Qin Zhang Key Laboratory of Animal Genetics and Breeding of the Ministry of agriculture, college of Animal {2. and Technology, China Agricultural university, Beijing 100193, China Key Laboratory of Animal Genetics and Breeding of the Ministry of agriculture College of Animal Science and Technology China Agricultural University Beijing 100193 China

Heat shock proteins （HSP） can protect organisms and cells from thermal damage. In this study, we cloned the full length cDNA encoding the HSP83 protein （the homologue of HSP90） of Tribolium castaneum（red flour beetle）. The isolated cDNA contains the full coding sequence, a partial 5＇ untranslated region of 55 bp and the complete 3＇ untranslated region. We found the hsp83 gene is located on chromosome 5 of the T. castaneum genome. The predicted HSP83 protein sequence has a high similarity （on average 86.77%） with that of other insect species. The expression of the hsp83 gene in the whole body and in the ovary could be induced with heat stress （40℃ for 1 h） in newly hatched （within 3 h post emergence） and mature （10 days post emergence） beetles. Under normal conditions, the hsp83 expression in the ovary is about 3-fold higher than in the whole body at both stages. No significant difference in hsp83 expression was observed between the two ovarian developmental stages regardless if the beetles were treated with heat shock or not. The expression of the HSP83 protein in the whole body could also be induced with heat stress in newly hatched and mature beetles. However, in the ovary, HSP83 was only expressed in the follicle cells of mature beetles and not in newly hatched beetles, regardless if the beetles were treated with heat shock or not. Furthermore, the females were not able to produce mature oocytes after knock-down of the hsp83 expression by injecting dsRNA. These results suggest that the HSP83 protein is involved in protection against heat stress and could be involved in oogenesis during ovarian maturation of T. castaneum.

关键词： hsp83 cDNA cloning Tribolium castaneum expression heat shock

Chemical Constituents from Frass of Daphnis neriireared on Catharanthus roseus

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Chemical Constituents from Frass of Daphnis neriireared on C...

The effects of Opuntia dillenii *** on treating fatty liver of hyperlipidemia rats

海南省药学会2.11年学术年会

作者： YANG Tao~(1,2.3),WU Jiao~(1,2.,ZENG Yan-Bo~(1,2.,ZUO Wen-Jian~(1,2.,MEI Wen-Li~(1,2.),DAI Hao-Fu~(1,2.) 1 hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural sciences, haikou 571101,China 2.haikou Key Laboratory for Research and Development of Tropical Natural Products,haikou 571101, China 3 Horticultural and Garden college,hainan university,haikou 5702.8,China

AIM:To study the bioactive constituents from the frass of Daphnis nerii reared on Catharanthus roseus ***:Compounds were isolated and purified by various chromatographic *** structures were elucidated on the basis of physicochemical properties and spectroscopic ***:Seven compounds were isolated and identified as serpentine(1), 3,4-dihydroxybenzoic acid(2.,sweroside(3),ursolic acid(4),ursolic acid-3-O-β- D-glucopyranoside(5), 3-O-(β-D-glucopyranosyl)ursolic acid-2.-O-(β-D-glucopyranosyl) ester(6),andβ-sitosterol(7). CONCLUSION:Compounds 1-7 were reported from the frass of *** reared on the *** leaves for the first *** 2.and 4 showed antibacterial activity,and compound 4 showed cytotoxic activity against K562.and SGC-7901.

关键词： Daphnis nerii Frass Chemical constituent Biological activity

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The effects of Opuntia dillenii *** on treating fatty liver ...

广西元宝山自然保护区蝴蝶种类组成及垂直分布

广东省植物学会第十七期学术研讨会

作者： HUANG Wei~(1,2. ZENG Fu-hua~(*2.1) RAO Li-qun~1 (1、college of Bioscience and Biotechnology,Hunan {2. university,Changsha,Hunan 41012.,China 2.School of life science and Technology,Zhanjiang Normal college,Zhanjiang,Guangdong 52.048,China)

The effects of Opuntia dillenii ***(OPS),which is one of the principal activation property of the Opuntia dillenii Haw.,on lipotropic were observed on hyperlipidemia rats in this *** hyperlipidemic fatty liver rats were induced through gavage with high fat emulsion for 10 *** was given to hyperlipidemie fatty liver rats through gavage for 2. ***,MDA content,the activity of SOD and HMG-CoA reductase in liver were exemined,and pathomorphology of liver tissue was *** a result,OPS could signifycantly got lower Liver index and liver lipid,OPS could significantly decrease MDA content and increase SOD activity in ***-CoA reductase activity was suppressed remarkably too. Moreover,OPS could improve antioxidative activity of hyperlipidemic fatty liver rats.

关键词： Opuntia dillenii Haw.Polysaccharide Hyperlipemia Fatty Liver

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四川动物 2003年第3期22卷 162-165页

作者：刘建文蒋国芳广西大学农学院南宁530004 南京师范大学生命科学学院

2. 0 1年 8月调查表明 ,目前广西元宝山自然保护区已知有蝴蝶 12.2. ,隶属于 11科 ,2.3亚科 ,85属 ;有 4种珍稀保护种类 ,发现了中国新纪录种———何华灰蝶。该保护区的蝴蝶呈现明显的垂直分布规律 ,从下往上划分为3个垂直分布带 :人... 详细信息

2. 0 1年 8月调查表明 ,目前广西元宝山自然保护区已知有蝴蝶 12.2. ,隶属于 11科 ,2.3亚科 ,85属 ;有 4种珍稀保护种类 ,发现了中国新纪录种———何华灰蝶。该保护区的蝴蝶呈现明显的垂直分布规律 ,从下往上划分为3个垂直分布带 :人工林与常绿阔叶林带、亚热带山地常绿阔叶林带。

关键词：广西元宝山自然保护区蝴蝶种类垂直分布

Regulation of ABA level and water-stress tolerance of Arabidopsis by ectopic expression of a stress-inducible gene for 9-cis-epoxycarotenoid dioxygenase in peanut plants

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Regulation of ABA level and water-stress tolerance of Arabid...