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Effects of chitosan/collagen substrates on the behavior of rat neural stem cells

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Science china(Life Sciences) 2010年第2期53卷 215-222页

作者： YANG ZhaoYang1,MO LinHong2.DUAN HongMei2.& Li XiaoGuang1,2.1 School of Biological Science and medical Engineering,Beihang university,{2. 100191,china 2.beijing Institute for neuroscience,capital medical university,beijing 100069,china 1. School of Biological Science and medical Engineering Beihang University Beijing 100191 China2. Beijing Institute for Neuroscience Capital Medical University Beijing 100069 China

Spinal cord and brain injuries usually lead to cavity *** transplantation by combining stem cells and tissue engineering scaffolds has the potential to fill the cavities and replace the lost neural *** chitosan and collagen have their unique *** this study,the effects of chitosan and collagen on the behavior of rat neural stem cells (at the neurosphere level) were tested in vitro in terms of cytotoxicity and supporting ability for stem cell survival,proliferation and *** the serum-free condition,both chitosan membranes and collagen gels had low cytotoxicity to *** is,cells migrated from neurospheres,and processes extended out from these neurospheres and the differentiated *** with the above two materials,chitosan-collagen membranes were more suitable for the co-culture with rat neural stem cells,because,except for low cytotoxicity and supporting ability for the cell survival,in this group,a large number of cells were observed to migrate out from neurospheres,and the differentiating percentage from neurospheres into neurons was significantly *** modification of chitosan-collagen membranes may shed light on in vivo nerve regeneration by transplanting neural stem cells.

关键词： chitosan collagen neural stem cells biocompatibility differentiation

Application of synchrotron source based DEI method in guinea pig cochleae imaging

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Progress in Natural Science:Materials International 2007年第1期17卷 59-67页

作者： YIN Hongxia, LIU Bo, GAO Xin, GAO Xiulai and LUO Shuqian ( College of Biomedical Engineering, capital {2. of medical Sciences, beijing 100069, china Department of Anatomy, capital {2. of medical Sciences, beijing 100069, china) College of Biomedical Engineering Capital University of Medical Sciences Beijing 100069 China Department of Anatomy Capital University of Medical Sciences Beijing 100069 China

Hard X-ray diffraction enhanced imaging (DEI), which is based on a synchrotron source and monochromator-analyzer-crystal system, is an effective method for imaging X-ray phase shift. Utilizing an analyzer crystal with high angular sensitivity of micro-radian, DEI can measure the transmitted, refracted and scattered X-rays when projecting onto a sample. It dramatically improves the contrast and spatial resolution of the resultant images. At the topography station of beijing Synchrotron Radiation Facilities (BSRF), we implemented DEI method in guinea pig cochleae imaging and acquired a series of DEI images. Based on these images, the apparent absorption and refraction images were calculated. The DEI images revealed the holistic spiral structures and inner details of guinea pig cochleae clearly, even including the structures at the cellular level, such as the static cilia of hairy cells and the limbus of Hansen cell. Due to the advantages of high contrast, high spatial resolution and distinct edge-enhanced effect, DEI method promises extensive applications in biology, medicine and clinic in the near future.

关键词： diffraction enhanced imaging hard X-ray imaging phase-contrast imaging cochlea micro-structure synchrotron source.

Effects of the CNTF-collagen gel-controlled delivery system on rat neural stem/progenitor cells behavior

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Science china(Life Sciences) 2010年第4期53卷 504-510页

作者： YANG ZhaoYang1, QIAO Hui2.& LI XiaoGuang1,3 1School of Biological Science and medical Engineering, Beihang university, {2. 100191, china 2.ublic Heath School, Ningxia medical university, Yinchuan 750004, china 3{2. Institute for neuroscience, capital medical university, beijing 100069, china 1. School of Biological Science and medical Engineering Beihang University Beijing 100191 China2. Public Heath School Ningxia Medical University Yinchuan 750004 China3. Beijing Institute for Neuroscience Capital Medical University Beijing 100069 China

The injury of central nervous system (CNS) usually causes the cavity formation. Although transplantation of neural stem/precursor cells (NSPCs) into the lesioned area of CNS has been shown to be implicated in the functional restoration, the therapeutic result is limited by the poor survival of NSPCs as well as their insufficient proliferation and differentiation abilities. Type-1 collagen is considered as a candidate scaffold or drug delivery system to overcome the aforementioned obstacle. This study observed the effects of the CNTF (ciliary neurotrophic factor)-collagen gel-controlled delivery system and daily addition of soluble-form CNTF on the NSPC survival, migration, proliferation and differentiation. The results showed that, within 12.h of the initial co-culture, CNTF was released in a burst pattern, then the CNTF-collagen gel-controlled delivery system stably released CNTF for up to 12.d. The cell viability test, together with immunohistochemistry, RT-PCR and Western blotting, showed that the CNTF-collagen gel-controlled delivery system supported the NSPCs seeded on the surface of collagen gel survival and facilitated their migration and proliferation. The daily addition of soluble-form CNTF to the medium had similar effects to the CNTF-collagen gel-controlled delivery system, but large quantities of soluble-form CNTF were consumed during the entire process. Taken together, the CNTF-collagen gel-controlled delivery system not only provides a physical scaffold for the transplanted NSPCs to adhere and migrate, but also facilitates the NSPC survival, growth and proliferation, simultaneously reducing the consumption of the expensive growth factors. This system may be used to enhance the microenvironment in the lesioned area of CNS.

关键词： spinal cord neural stem/progenitor cells control release type-1 collagen CNTF

Cu(Ⅱ) Potentiation of Alzheimer Aβ1-40 Cytotoxicity and Transition on its Secondary Structure

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 765-772页

作者： Xue-Ling DAI~1 Ya-Xuan SUN~2.Zhao-Feng JIANG~2. 1 College of Life Science,capital Normal university,{2. 100037,china 2.College of Applied Sciences and Humanities,{2. Union university,{2. 100083,china College of Life Science Capital Normal UniversityBeijing 100037 China College of Applied Sciences and Humanities Beijing Union UniversityBeijing 100083 China

Mounting evidence has shown that dyshomeostasis of the redox-active biometals such as Cuand Fe can lead to oxidative stress,which plays a key role in the neuropathology of Alzheimer's disease（AD）.Here we demonstrate that with the formation of Cu（Ⅱ）·Aβ1-40 complexes,copper markedly potentiatesthe neurotoxicity exhibited by β-amyloid peptide （Aβ）.A greater amount of hydrogen peroxide was releasedwhen Cu（Ⅱ）·Aβ1-40 complexes was added to the xanthine oxidase/xanthine system detected by potassiumiodide *** bound to Aβ1-40 was observed by electron paramagnetic resonance（EPR） *** dichroism （CD） studies indicated that copper chelation could cause a structuraltransition of Aβ.The addition of copper to Aβ introduced an increase on β-sheet as well as α-helix,whichmay be responsible for the aggregation of Aβ.We hypothesized that Aβ aggregation induced by copper maybe responsible for local injury in *** interaction between Cu2.and Aβ also provides a possible mechanismfor the enrichment of metal ions in amyloid plaques in the AD brain.

关键词： Alzheimer’s disease Cu(Ⅱ)·Aβ1-40 complex neuron injury Aβ secondary structure

Protective effect of liver ischemic preconditioning on rat hepatocytes

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Science china(Life Sciences) 2009年第9期52卷 836-840页

作者： FU JianZhu1,2.WANG Yu1,ZHANG LiJun2.& YU ZeLi2.1 Department of General Surgery,beijing Friendship Hospital,capital medical university,beijing 100050,china 2.Department of General Surgery,beijing Tongren Hospital,capital medical university,beijing 100730,china 1. Department of General Surgery Beijing Friendship Hospital Capital Medical University Beijing 100050 China 2. Department of General Surgery Beijing Tongren Hospital Capital Medical University Beijing 100730 China

Ischemic preconditioning (IPC) protects liver graft function following ischemia in liver transplantation and liver resection. The aim of this study was to assess the advantages and any potential disadvantages of liver IPC prior to isolation for rat hepatocytes during isolation and cryopreservation. After isolating and thawing the cryopreserved hepatocytes after 14 and 2. d,cell viability,efficiency,and lactate dehydrogenase (LDH) levels in preserve solution were examined for every group. Groups treated with IPC had better cell viability determination,assessment of plating efficiency and lactate dehydrogenase (LDH) assay than Group without IPC,suggesting that IPC prior to isolation may have a significant protective effect on hepatocytes subjected to isolation and short period cryopreservation.

关键词： liver ischemic preconditioning hepatocytes cryopreservation

Interferon-α induced severe thrombocytopenia:A case report and review of the literature

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World Journal of Gastroenterology 2010年第11期16卷 1414-1417页

作者： Li Li,Da-Kang Han,Jun Lu,Tumor Biotherapy Ward of beijing Youan Hospital,capital medical university,{2.,china Tumor Biotherapy Ward of beijing Youan Hospital Capital Medical University Beijing 100069 China. lujun98@***

We report a case of severe thrombocytopenia following pegylated interferon-α 2.(Peg-IFN-α 2.)treatment of hepatitis C virus infection and summarize the clinical characteristics of 16 cases of IFN-α induced severe thrombocytopenia and its immune-mediated *** of IFN-α and early administration of immunosuppressants are the effective therapy for IFN-αinduced severe thrombocytopenia.

关键词： Interferon-α Severe thrombocytopenia Chronic hepatitis C

The numerical method for three-dimensional impact with friction of multi-rigid-body system

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Science china(Physics,Mechanics & Astronomy) 2006年第1期49卷 102-118页

作者： ZHAO Zhen1,2. LIU Caishan2.& CHEN Bin2.1. beijing Institute of Graphic Communication, beijing 102.00, {2. 2. Department of Mechanics & Engineering Science, Peking university, beijing 100871, {2. beijing Institute of Graphic Communication Beijing 102600 China Department of Mechanics & Engineering Science Peking University Beijing 100871 China

The differential equations for planar impacts reduce to an algebraic form, and can be easily solved. For three dimensional impacts with friction, there is no closed-form solution, and numerical integration is required due to the swerve behavior of tangential impulse during collisions. The dynamic governing equations in the impact process are built up in impulse space based on the Lagrangian equation in this paper. The coefficient of restitution defined by Poisson is used as the condition of impact termination. A valid nu- merical method for solving three-dimensional frictional impact of multi-rigid body system is established. The singular cases of tangential movement in sticking point are especially noticed and analyzed. Several examples are present to reveal the different kinds of tan- gential movement modes varied with the normal impulse during collision.

关键词： multi-rigid body, impact, friction.

Flotation Separating Rutile from Calcium and Silicon Gangue Minerals

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Rare Metals 2001年第4期20卷 205-208页

作者： Hao Ding1, HaiLin 2.1) beijing Academy of Building Materials, beijing 100041, {2. 2. university of Science and Technology beijing, beijing 100083, {2. beijing Acad Bldg Mat Beijing 100041 Peoples R China Univ Sci & Technol beijing Beijing 100083 Peoples R China

Rutile separation from calcite, apatite and quartz by flotation was investigated. The results show that the rutile separation from calcium and silicon gangue minerals can be realized with alkyl-imino-bismethylene phos... 详细信息

关键词： rutile calcium and silicon gangue minerals flotation separate alkyl-imino-bismethylene phosphoric acid sodium hexametaphosphate

Density-functional formula for strongly correlated systems

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Progress in Natural Science:Materials International 2005年第5期15卷 395-401页

作者： WANG Huaiyu1*, HAN Rushan2.and CHEN Nanxian1(1. Department of Physics, Tsinghua university, beijing 100084, {2. 2. Department of Physics, Peking university, beijing 100871, {2.) Department of Physics Tsinghua University Beijing 100084 China Department of Physics Peking University Beijing 100871 China

Density functional method is applied for strongly correlated systems. Based on the assumption that the systems are composed of electrons in singly-occupied orbitals and those in doubly-occupied orbitals, a set of self-consistent equations are obtained by standard variation procedure. The equations consist of two parts. One part is to solve the wave functions of the electrons in singly-occupied orbitals and the other is to solve the wave functions of the electrons in doubly-occupied orbitals. The physical meanings of the terms appearing in the equations are discussed.

关键词： density-functional theory strongly correlated systems doubly-occupied orbital two-particle wave function self-consistent equations.